Description of fluorescence parameters measured using CR10X algorithm and PAM-2000 user programs.
Key to PAM Activity Labels
ML= Measuring Light
FR= Far Red light
AL= Actinic Light
SP= Saturation Pulse
Description of Parameters Measured on FLR Traces
1D,L=5 second Fluorescence average with measure light on , actinic light off.
2D,L= 5 second fluorescence average with measure light on, Far Red on, Actinic off.
3D= 10 point 20 msec Fluorescence average immediately prior to saturation pulse, measure light on, Far Red off, Actinic light off.
3L= 10 point 20 msec Fluorescence average immediately prior to saturation pulse, measure light on, Far Red off, Actinic light on.
4D,L=10 point 20 msec Fluorescence average surrounding peak fluorescence of saturation pulse, measure light on, Far Red off.
Fo= 1D= parameter 1 of dark adapted state
Fo’= 2L= parameter 2 of light adapted state
Fm=4D= parameter 4 of dark adapted state
Fm’=4L= parameter 4 of light adapted state
F=3L=parameter 3 of light adapted state, basal fluorescence in light
Translation of CR10X output variables to van Kooten and Snell 1990 & Maxwell and Johnson 2000 Fluorescence variables
Fmin: ‘F’
Fmax: Fm
Fo0: Fo
Foprim:Fo’
Yield: maximum quantum yield
Fv/Fmax: quantum yield of PSII
Useful Fluorescence Quenching Equations
PAM-2000 User Runs for use with CR10X Fluorometer Control Program
These commands should be input into the PAM-2000 as user modified RUN files. The PAM-2000 manual provides instructions how to do this. These commands are carefully timed to correspond with the data logging activities of the CR10X program in Appendix B; and any modification of these user runs will necessitate changes in the CR10X program within subroutine 1. With an understanding of how subroutine 1 operates,it is easy to changethe timing of sampling Fo and Fo’ and the delay prior to initiating a saturation pulse.
Run 2: This user run should be used only with samples in the dark and with the measuring light off at the start of the run. At the end of the run the sample will remain in the dark and the measuring light will be off. PAM settings at start of run (measuring light off, actinic light off).
#2
1,N
1,L
9,F
12,Z
17,L
17,F
22,Y
25,L
25^R
Run3: This user run should be used for samples in the dark and with the measuring light on at the start of the run. At the end of the run the sample will remain in the dark and the measuring light will be on. PAM settings at start of run (measuring light on, actinic light off).
#3
1,N
9,F
12,Z
17,L
17,F
22,Y
25^R
Run 4: This user run should be used for samples when the actinic light is on and the measuring light is off at the start of the run. At the end of the run the sample will remain illuminated by actinic light and the measuring light will be off. PAM settings at start of run (measuring light off, actinic light on).
#4
1,N
1,A
1,L
9,F
12,Z
17,L
17,L
17,A
22,Y
25,L
25^R
Run 5: This user run should be used for samples when the actinic light is on and the measuring light is on at the start of the run. At the end of the run the sample will remain illuminated by actinic light and the measuring light will be on. PAM settings at start of run (measuring light on, actinic light on).
#5
1,N
1,A
9,F
12,Z
17,L
17,F
17,A
22,Y
25,^R