Table S1. Germination rates of sunflower broomrape seeds conditioned with water or GA3 in response to GR24 at concentrations of 0.1 mg L-1 and 1 mg L-1 over 21 days of conditioning.

Conditioning period(days) / 0.1 mg/L GR24 / 1 mg/L GR24
100 μM GA3 / 10 μM GA3 / 1 μM GA3 / distilled water / 100 μM GA3 / 10 μM GA3 / 1 μM GA3 / distilled water
0 / 14.0±1.4 / 14.0±1.4 / 14.0±1.4 / 14.0±1.4 / 33.0±4.5 / 33.0±4.5 / 33.0±4.5 / 33.0±4.5
1 / 57.1±7.8 / 29.5±9.2 / 10.3±9.2 / 32.8±3.6 / 63.7±5.3 / 43.8±6.2 / 64.3±6.2 / 38.3±4.0
2 / 62.7±5.6 / 42.8±7.4 / 21.5±7.4 / 52.7±6.1 / 65.9±2.4 / 51.7±3.7 / 65.7±3.7 / 67.1±3.7
3 / 68.0±8.7 / 41.0±5.6 / 44.1±5.6 / 62.4±1.4 / 71.7±3.1 / 56.2±3.5 / 71.2±3.5 / 67.5±2.8
4 / 76.8±1.9 / 68.5±7.0 / 55.0±7.0 / 58.0±7.3 / 85.5±7.7 / 62.0±3.6 / 77.7±3.6 / 57.7±4.5
5 / 84.3±8.5 / 76.6±2.4 / 64.4±2.4 / 54.6±7.6 / 62.1±2.6 / 77.1±11.0 / 78.6±11.0 / 59.2±4.2
6 / 78.5±3.7 / 81.7±3.4 / 57.5±3.4 / 61.7±2.4 / 56.3±2.6 / 72.4±4.3 / 83.8±4.3 / 65.4±3.6
7 / 89.0±3.0 / 77.8±7.3 / 40.3±7.3 / 66.4±7.2 / 71.4±3.3 / 73.0±2.9 / 77.0±2.9 / 66.4±4.0
8 / 84.1±1.2 / 77.4±4.8 / 33.8±4.8 / 58.7±1.1 / 86.9±4.1 / 86.7±2.6 / 69.1±2.6 / 68.9±5.4
9 / 80.7±2.0 / 76.9±4.4 / 37.1±4.4 / 57.4±2.4 / 80.2±4.1 / 91.1±3.3 / 69.6±3.3 / 58.3±5.6
10 / 80.6±2.5 / 67.4±3.9 / 39.1±3.9 / 46.2±3.8 / 75.7±3.8 / 71.2±8.5 / 73.2±8.5 / 47.0±5.8
11 / 87.6±5.0 / 57.0±6.0 / 45.7±6.0 / 51.9±3.3 / 77.1±4.9 / 74.8±4.2 / 80.1±4.2 / 47.9±0.6
12 / 87.1±7.6 / 47.3±7.9 / 21.9±7.9 / 36.3±4.5 / 74.4±1.5 / 84.8±8.0 / 69.7±8.0 / 34.2±2.3
13 / 77.8±7.5 / 44.2±4.2 / 32.1±4.2 / 36.6±2.1 / 69.3±1.5 / 90.4±5.4 / 70.4±5.4 / 45.0±4.9
14 / 82.3±4.6 / 32.4±6.0 / 25.9±6.0 / 36.0±3.0 / 77.5±3.8 / 88.5±6.0 / 69.4±6.0 / 55.4±2.9
15 / 80.7±4.8 / 28.7±4.2 / 13.8±4.2 / 35.0±3.7 / 78.4±2.9 / 62.5±8.5 / 68.4±8.5 / 53.1±4.1
16 / 83.0±3.7 / 35.3±8.3 / 18.9±8.3 / 38.4±3.0 / 64.4±0.8 / 70.2±4.1 / 66.4±4.1 / 43.5±4.2
17 / 83.6±9.0 / 27.7±4.2 / 10.3±4.2 / 44.2±5.4 / 72.3±3.5 / 84.4±6.1 / 68.9±6.1 / 49.3±3.5
18 / 84.6±7.0 / 44.2±6.2 / 19.7±6.2 / 46.2±4.7 / 82.4±8.8 / 79.5±4.8 / 68.3±4.8 / 67.5±5.6
19 / 77.2±5.1 / 36.5±2.8 / 29.2±2.8 / 31.1±5.1 / 79.7±1.7 / 76.9±8.9 / 70.1±8.9 / 37.7±4.3
20 / 69.7±4.2 / 28.3±7.3 / 28.3±7.3 / 32.2±3.6 / 79.2±7.4 / 70.4±2.8 / 71.8±2.8 / 27.7±1.7
21 / 73.5±3.6 / 25.6±5.6 / 38.9±5.6 / 27.9±4.0 / 68.9±4.4 / 64.8±3.3 / 62.3±3.3 / 26.6±3.9

Data represent the mean ± SE (n = 10)

TableS2. Germination rates of sunflower broomrape seeds conditioned with water or GA3 in response to strigol at concentrations of 0.1 mg L-1 and 1 mg L-1 over 21 days of conditioning.

Conditioning period(days) / 0.1 mg/L strigol / 1 mg/L strigol
100 μM GA3 / 10 μM GA3 / 1 μM GA3 / distilled water / 100 μM GA3 / 10 μM GA3 / 1 μM GA3 / distilled water
0 / 5.0±1.5 / 5.0±1.5 / 5.0±1.5 / 5.0±1.5 / 26.9±5.9 / 26.9±5.9 / 26.9±5.9 / 26.9±5.9
1 / 77.9±9.5 / 32.0±5.6 / 25.2±5.6 / 13.3±1.4 / 84.4±1.5 / 66.1±6.3 / 46.4±6.3 / 36.3±2.3
2 / 94.9±1.0 / 33.7±4.4 / 32.0±4.4 / 21.8±5.9 / 83.6±8.7 / 84.2±3.1 / 45.5±3.1 / 57.8±7.9
3 / 91.5±1.8 / 71.2±4.4 / 35.3±4.4 / 50.5±3.3 / 80.8±8.3 / 88.3±9.5 / 66.4±9.5 / 59.1±7.4
4 / 92.0±0.7 / 79.9±4.0 / 53.7±4.0 / 60.6±2.6 / 82.2±6.1 / 92.0±1.7 / 70.0±1.7 / 57.0±1.5
5 / 91.9±4.3 / 88.5±4.8 / 59.9±4.8 / 76.5±4.7 / 78.9±8.3 / 85.0±5.1 / 72.3±5.1 / 55.8±6.4
6 / 85.3±2.9 / 81.0±8.1 / 56.0±8.1 / 88.2±4.6 / 76.1±3.0 / 87.1±4.9 / 68.5±4.9 / 62.7±1.6
7 / 95.9±1.5 / 84.4±3.8 / 42.3±3.8 / 75.8±4.5 / 93.1±1.5 / 82.9±8.2 / 70.5±8.2 / 65.9±5.8
8 / 95.0±1.9 / 62.5±7.2 / 36.9±7.2 / 44.8±1.8 / 82.7±7.5 / 78.2±7.5 / 50.9±7.5 / 56.0±1.1
9 / 96.5±0.6 / 70.1±5.0 / 57.7±5.0 / 48.9±9.2 / 92.9±2.4 / 90.5±3.2 / 61.5±3.2 / 66.4±5.0
10 / 96.3±1.5 / 69.4±5.5 / 50.4±5.5 / 53.0±7.9 / 83.6±3.0 / 85.3±5.3 / 68.4±5.3 / 74.0±8.3
11 / 96.8±0.4 / 72.3±7.4 / 44.1±7.4 / 61.5±4.2 / 85.3±2.2 / 83.7±9.3 / 75.5±9.3 / 67.9±5.1
12 / 76.8±2.7 / 63.6±1.9 / 51.4±1.9 / 62.1±3.0 / 90.6±5.7 / 91.1±1.3 / 64.2±1.3 / 58.0±14.8
13 / 77.8±6.0 / 46.1±3.6 / 34.6±3.6 / 63.3±4.0 / 95.1±1.1 / 86.8±5.2 / 52.9±5.2 / 66.8±4.4
14 / 90.5±0.5 / 41.2±3.6 / 17.9±3.6 / 55.1±9.1 / 93.0±2.7 / 90.7±4.0 / 57.0±4.0 / 65.2±3.8
15 / 95.6±0.8 / 36.2±4.0 / 31.4±4.0 / 53.4±5.7 / 83.0±7.2 / 86.4±7.2 / 40.5±7.2 / 60.0±5.2
16 / 90.7±0.8 / 35.5±2.5 / 20.4±2.5 / 48.2±5.9 / 94.2±0.5 / 81.9±5.4 / 57.2±5.4 / 60.8±4.4
17 / 74.0±3.4 / 28.9±6.1 / 11.7±6.1 / 42.3±5.2 / 85.1±6.6 / 89.0±4.3 / 57.6±4.3 / 52.7±2.7
18 / 76.3±6.2 / 37.6±9.8 / 29.8±9.8 / 39.6±2.3 / 91.2±0.3 / 96.5±8.7 / 54.5±8.7 / 44.0±7.7
19 / 95.2±0.8 / 43.0±3.6 / 34.8±3.6 / 32.3±2.9 / 89.3±4.5 / 92.1±0.4 / 57.4±0.4 / 41.3±3.6
20 / 96.8±0.4 / 48.5±5.0 / 33.3±5.0 / 34.0±3.4 / 85.7±2.9 / 78.4±5.4 / 48.3±5.4 / 33.4±8.5
21 / 95.0±0.8 / 41.0±6.8 / 21.6±6.8 / 33.4±6.4 / 93.4±2.0 / 80.5±5.3 / 59.8±5.3 / 35.0±6.4

Data represent the mean ± SE (n = 10)

TableS3. Germination rates of sunflower broomrapeseeds conditioned with water or GA3 in response to DCL at concentrations of 0.1 mg L-1 and 1 mg L-1 over 21 days of conditioning.

Conditioning period(days) / 0.1 mg/L DCL / 1 mg/L DCL
100 μM GA3 / 10 μM GA3 / 1 μM GA3 / distilled water / 100 μM GA3 / 10 μM GA3 / 1 μM GA3 / distilled water
0 / 0.0±0.0 / 0.0±0.0 / 0.0±0.0 / 0.0±0.0 / 26.8±5.0 / 26.8±5.0 / 26.8±5.0 / 26.8±5.0
1 / 34.3±6.2 / 18.9±5.9 / 14.9±0.7 / 9.6±0.7 / 52.8±3.2 / 39.5±3.5 / 34.2±2.3 / 31.1±2.3
2 / 19.4±2.7 / 28.2±7.5 / 15.0±9.1 / 18.7±9.1 / 55.1±3.4 / 37.6±6.7 / 62.5±6.6 / 51.0±6.6
3 / 24.1±6.3 / 32.8±1.0 / 24.8±5.5 / 14.4±5.5 / 70.1±11.1 / 67.9±3.3 / 62.1±6.4 / 57.8±6.4
4 / 47.6±4.5 / 44.4±4.5 / 44.0±6.2 / 21.6±6.2 / 60.5±4.6 / 59.5±7.2 / 59.6±5.8 / 52.0±5.8
5 / 51.3±3.3 / 49.4±7.5 / 36.1±4.4 / 31.6±4.4 / 55.6±3.0 / 63.7±2.8 / 71.7±6.5 / 61.7±6.5
6 / 69.0±4.5 / 45.3±4.1 / 33.2±2.9 / 34.7±2.9 / 74.4±6.7 / 71.5±4.5 / 87.2±5.5 / 61.4±5.5
7 / 55.9±3.2 / 42.3±5.6 / 25.5±7.2 / 31.7±7.2 / 65.6±1.7 / 71.0±6.1 / 80.3±7.0 / 63.6±7.0
8 / 50.3±2.2 / 49.7±8.0 / 15.9±2.9 / 19.2±2.9 / 58.6±2.4 / 71.7±2.9 / 73.4±4.1 / 61.2±4.1
9 / 49.9±6.4 / 52.0±4.2 / 18.3±6.0 / 32.1±6.0 / 55.4±7.8 / 72.3±2.5 / 87.7±3.4 / 54.5±3.4
10 / 49.6±1.1 / 24.1±4.9 / 16.3±2.1 / 26.8±2.1 / 55.0±2.4 / 60.4±7.3 / 81.3±8.5 / 55.4±8.5
11 / 31.1±7.7 / 23.4±6.0 / 28.6±2.1 / 26.0±2.1 / 58.8±3.0 / 68.4±2.8 / 69.8±3.3 / 61.0±3.3
12 / 46.2±0.7 / 17.1±6.9 / 15.1±0.7 / 9.9±0.7 / 72.2±2.4 / 55.0±2.6 / 47.1±5.4 / 60.8±5.4
13 / 64.0±2.0 / 17.9±6.6 / 11.1±2.6 / 10.8±2.6 / 70.7±3.4 / 53.5±1.1 / 37.5±2.6 / 61.3±2.6
14 / 68.0±1.7 / 18.5±7.5 / 13.9±3.0 / 6.9±3.0 / 46.4±3.4 / 63.0±2.5 / 45.3±4.2 / 65.3±4.2
15 / 70.6±4.9 / 26.3±3.0 / 19.1±9.7 / 12.5±9.7 / 45.9±3.7 / 77.2±7.2 / 49.5±8.3 / 67.3±8.3
16 / 61.3±0.4 / 21.2±4.9 / 5.5±4.1 / 3.0±4.1 / 45.4±4.8 / 61.0±5.7 / 33.3±3.0 / 43.2±3.0
17 / 42.0±4.0 / 22.2±6.4 / 6.4±7.2 / 4.0±7.2 / 67.6±3.1 / 58.2±3.6 / 23.6±1.0 / 41.7±1.0
18 / 40.4±1.6 / 12.7±3.5 / 9.4±2.7 / 13.1±2.7 / 51.9±7.9 / 53.1±3.1 / 33.1±2.1 / 36.9±2.1
19 / 38.9±3.3 / 13.3±3.7 / 7.2±4.2 / 11.1±4.2 / 69.5±5.8 / 64.6±3.8 / 44.0±3.7 / 35.6±3.7
20 / 45.1±0.9 / 24.1±6.0 / 8.4±7.9 / 5.0±7.9 / 70.6±3.2 / 64.8±8.4 / 23.0±0.8 / 28.9±0.8
21 / 60.5±1.8 / 9.4±4.4 / 18.8±1.4 / 11.4±1.4 / 85.8±5.2 / 66.9±5.2 / 51.7±6.4 / 23.1±6.4

Data represent the mean ± SE (n = 10)

TableS4. Germination rates of sunflower broomrapeseeds conditioned with water or GA3 in response to maize root exudates at concentrations of 1 mg L-1 and 10 mg L-1 over 21 days of conditioning.

Conditioning period(days) / 1 mg/Lmaize root exudates / 10 mg/L maize root exudates
100 μM GA3 / 10 μM GA3 / 1 μM GA3 / distilled water / 100 μM GA3 / 10 μM GA3 / 1 μM GA3 / distilled water
0 / 0.0±0.0 / 0.0±0.0 / 0.0±0.0 / 0.0±0.0 / 10.2±1.0 / 10.2±1.0 / 10.2±1.0 / 10.2±1.0
1 / 61.0±4.2 / 50.2±4.4 / 28.4±2.0 / 5.2±0.2 / 77.8±4.6 / 77.8±2.7 / 62.6±2.3 / 36.2±2.8
2 / 67.4±3.3 / 77.8±4.0 / 62.4±3.2 / 32.4±3.4 / 70.4±5.1 / 75.6±2.4 / 79.8±3.5 / 59.2±4.3
3 / 90.4±1.6 / 70.2±2.7 / 60.2±4.2 / 35.6±2.4 / 88.2±2.7 / 75.6±2.9 / 83.4±2.8 / 69.2±3.4
4 / 87.6±1.9 / 69.8±2.0 / 64.2±2.9 / 38.0±2.7 / 88.2±2.0 / 77.4±2.2 / 72.8±1.0 / 72.4±4.5
5 / 65.0±4.3 / 78.8±2.8 / 67.2±4.0 / 42.6±4.9 / 77.8±3.0 / 84.0±3.7 / 84.4±3.7 / 66.6±3.0
6 / 82.4±3.7 / 86.2±2.7 / 71.6±4.0 / 36.0±3.4 / 86.8±2.5 / 86.2±3.6 / 79.8±8.8 / 67.2±1.7
7 / 72.8±3.5 / 58.6±5.0 / 53.4±2.3 / 44.4±2.9 / 82.2±2.2 / 65.0±3.2 / 78.0±3.0 / 55.2±1.3
8 / 71.2±2.6 / 51.0±4.2 / 51.0±4.2 / 51.0±4.2 / 84.2±1.8 / 79.4±2.4 / 79.4±2.4 / 79.4±2.4
9 / 70.0±6.2 / 70.2±6.3 / 67.8±1.5 / 24.4±2.1 / 86.6±1.8 / 88.6±1.0 / 75.8±2.2 / 73.0±1.2
10 / 85.0±1.6 / 78.6±2.4 / 55.6±2.5 / 12.6±2.8 / 89.2±2.9 / 86.6±4.2 / 76.4±2.7 / 59.2±4.0
11 / 85.4±2.1 / 79.4±2.4 / 79.6±3.0 / 8.8±1.2 / 81.8±6.5 / 86.8±3.2 / 84.0±2.6 / 62.0±4.9
12 / 69.0±3.8 / 80.4±3.1 / 70.6±2.4 / 6.4±0.2 / 75.0±3.6 / 84.0±2.9 / 79.8±2.2 / 37.4±4.0
13 / 73.2±5.8 / 61.0±2.2 / 34.0±4.2 / 9.6±0.7 / 70.8±1.7 / 74.4±3.7 / 68.4±3.0 / 46.6±3.9
14 / 69.6±5.0 / 67.8±3.2 / 39.2±3.7 / 3.2±0.7 / 81.2±3.5 / 84.0±2.9 / 82.2±3.8 / 21.4±3.4
15 / 75.2±4.5 / 64.2±7.0 / 29.8±7.9 / 3.2±0.4 / 79.6±1.6 / 78.6±3.1 / 78.2±2.0 / 33.8±3.5
16 / 84.2±3.5 / 60.4±7.1 / 25.4±1.4 / 4.0±1.2 / 89.4±2.6 / 81.8±3.4 / 82.4±1.7 / 35.4±3.0
17 / 62.6±3.6 / 58.8±6.4 / 24.8±2.6 / 4.4±0.2 / 79.2±4.6 / 81.8±3.4 / 74.8±3.6 / 25.0±2.0
18 / 61.4±5.4 / 50.6±5.1 / 17.8±3.0 / 3.8±0.7 / 83.4±0.8 / 79.4±4.6 / 65.2±10.2 / 17.4±4.2
19 / 82.6±2.4 / 80.2±2.0 / 15.4±2.5 / 2.8±0.9 / 89.8±2.4 / 89.2±1.9 / 64.2±3.8 / 22.8±3.7
20 / 68.6±4.2 / 52.0±1.0 / 11.2±0.7 / 6.8±1.7 / 70.6±5.7 / 75.2±3.3 / 66.6±6.1 / 20.0±4.5
21 / 55.6±8.4 / 38.8±2.5 / 18.2±2.1 / 0.0±0.0 / 86.8±2.9 / 77.0±6.3 / 67.2±2.9 / 16.0±3.7

Data represent the mean ± SE (n = 10)

Table S5. Germination rates of sunflower broomrape seeds conditioned with water or GA3 in response to tobacco root exudates at concentrations of 1 mg L-1 and 10 mg L-1 over 21 days of conditioning.

Conditioning period(days) / 1 mg/L tobacco root exudates / 10 mg/L tobacco root exudates
100 μM GA3 / 10 μM GA3 / 1 μM GA3 / distilled water / 100 μM GA3 / 10 μM GA3 / 1 μM GA3 / distilled water
0 / 0.0±0.0 / 0.0±0.0 / 0.0±0.0 / 0.0±0.0 / 0.0±0.0 / 0.0±0.0 / 0.0±0.0 / 0.0±0.0
1 / 32.8±3.4 / 43.4±3.3 / 25.2±3.1 / 3.0±0.3 / 41.0±2.0 / 44.8±1.5 / 8.8±1.2 / 8.0±2.3
2 / 81.4±5.8 / 70.0±4.7 / 62.2±2.1 / 12.4±3.8 / 86.0±2.6 / 83.8±1.5 / 69.0±1.4 / 29.4±2.4
3 / 75.0±5.6 / 69.8±2.6 / 70.2±4.1 / 12.2±3.6 / 75.0±2.6 / 75.2±4.3 / 62.2±2.9 / 37.8±0.4
4 / 73.6±3.5 / 70.4±2.8 / 56.4±4.8 / 23.2±2.8 / 82.6±2.6 / 80.0±2.0 / 63.4±2.4 / 28.6±3.5
5 / 79.8±4.5 / 81.4±1.7 / 56.2±4.3 / 18.0±2.2 / 71.8±2.9 / 77.0±6.9 / 53.8±0.8 / 27.0±1.9
6 / 84.4±4.4 / 76.8±3.6 / 58.8±3.9 / 9.4±4.3 / 91.2±0.9 / 89.6±1.1 / 57.4±2.5 / 16.8±1.3
7 / 74.4±3.7 / 63.8±1.9 / 30.8±7.1 / 6.0±4.5 / 83.4±2.9 / 75.8±2.9 / 43.0±5.0 / 17.0±1.0
8 / 82.4±2.5 / 74.2±4.3 / 43.4±1.2 / 12.6±1.2 / 70.4±12.4 / 85.0±1.8 / 41.2±2.7 / 13.4±2.9
9 / 80.2±2.7 / 85.2±1.9 / 48.0±6.6 / 5.0±3.0 / 87.8±3.1 / 93.2±2.5 / 40.2±1.2 / 17.8±1.1
10 / 79.4±5.0 / 82.0±1.5 / 49.6±3.6 / 7.2±2.3 / 90.6±0.9 / 84.2±2.6 / 57.4±2.3 / 12.2±0.7
11 / 81.6±6.2 / 79.2±2.8 / 59.2±4.3 / 2.8±2.3 / 89.0±1.9 / 84.6±2.2 / 51.8±5.5 / 8.6±0.4
12 / 72.2±3.9 / 43.2±4.5 / 23.6±5.0 / 6.4±4.5 / 77.8±3.2 / 80.8±3.6 / 58.2±9.1 / 13.6±0.9
13 / 74.2±4.5 / 27.6±3.5 / 16.6±4.7 / 2.0±0.0 / 86.4±1.9 / 68.6±4.0 / 40.2±2.7 / 4.0±0.0
14 / 66.2±5.3 / 27.4±2.3 / 7.8±1.9 / 4.0±0.0 / 82.4±0.9 / 73.4±3.3 / 34.2±3.4 / 2.8±1.1
15 / 68.0±2.8 / 21.0±4.2 / 6.0±1.6 / 2.0±0.0 / 85.8±2.6 / 63.6±3.2 / 9.6±2.7 / 9.2±2.1
16 / 53.6±4.5 / 25.0±2.2 / 7.2±1.2 / 2.0±0.0 / 90.2±1.1 / 71.0±6.6 / 10.4±2.0 / 3.2±1.1
17 / 59.0±0.9 / 11.8±1.5 / 6.8±1.2 / 3.0±0.5 / 85.8±2.5 / 67.2±2.6 / 17.2±1.2 / 4.2±0.8
18 / 37.6±2.3 / 7.8±2.5 / 6.4±1.2 / 0.0±0.0 / 76.4±4.1 / 60.2±7.0 / 8.6±1.1 / 3.0±0.3
19 / 37.6±3.4 / 6.4±1.6 / 7.0±1.4 / 0.0±0.0 / 87.0±2.6 / 45.0±4.6 / 11.6±1.7 / 5.0±0.0
20 / 41.4±5.6 / 10.6±2.0 / 4.2±0.8 / 0.0±0.0 / 81.8±2.3 / 27.8±1.7 / 8.0±2.8 / 4.0±0.3
21 / 33.4±3.9 / 5.8±1.1 / 3.2±1.5 / 0.0±0.0 / 80.2±5.0 / 13.0±1.4 / 4.6±0.8 / 4.0±0.0

Data represent the mean ± SE (n = 10)

Table S6. Germination rates of Egyptian broomrape seeds conditioned with water or GA3 in response to GR24 at concentrations of 0.1 mg L-1 and 1 mg L-1 over 21 days of conditioning.

Conditioning period (days) / 0.1 mg/L GR24 / 1 mg/L GR24
100 μM GA3 / 10 μM GA3 / 1 μM GA3 / distilled water / 100 μM GA3 / 10 μM GA3 / 1 μM GA3 / distilled water
0 / 58.2±4.5 / 58.2±4.5 / 58.2±4.5 / 58.2±4.5 / 51.9±3.8 / 51.9±3.8 / 51.9±3.8 / 51.9±3.8
1 / 81.5±8.7 / 67.4±6.6 / 78.5±1.5 / 69.7±1.5 / 75.3±4.7 / 85.5±4.9 / 72.7±5.3 / 63.1±8.6
2 / 73.4±7.3 / 90.8±4.1 / 73.9±6.4 / 81.1±9.0 / 70.2±4.7 / 95.2±4.8 / 75.2±5.1 / 84.5±5.7
3 / 70.6±4.3 / 93.9±1.5 / 79.9±3.6 / 79.9±5.9 / 74.7±8.0 / 87.5±2.3 / 67.6±3.2 / 83.3±6.6
4 / 80.2±3.1 / 92.8±0.8 / 91.3±4.2 / 91.5±2.4 / 76.5±5.5 / 79.4±3.6 / 68.7±4.1 / 94.0±2.0
5 / 82.5±4.1 / 88.8±6.5 / 88.3±3.5 / 85.7±4.9 / 78.2±6.3 / 88.8±3.6 / 85.6±2.4 / 86.2±0.6
6 / 80.6±5.9 / 96.1±0.8 / 88.8±0.8 / 85.2±4.3 / 69.5±4.0 / 87.8±1.2 / 79.9±0.9 / 80.4±3.7
7 / 78.6±5.1 / 91.5±1.7 / 88.6±5.4 / 76.1±2.5 / 83.7±6.1 / 76.6±4.5 / 78.2±3.7 / 76.0±6.3
8 / 72.7±7.1 / 85.7±4.2 / 82.3±4.1 / 73.1±10.0 / 85.1±4.0 / 77.3±4.9 / 80.5±6.2 / 77.9±5.2
9 / 75.8±6.6 / 79.9±6.0 / 92.2±2.7 / 81.5±3.2 / 85.9±7.9 / 77.9±4.5 / 80.1±4.4 / 75.4±13.5
10 / 68.5±7.0 / 71.1±5.0 / 60.8±9.8 / 88.3±3.9 / 83.0±3.6 / 79.7±4.2 / 67.6±4.3 / 72.8±4.0
11 / 64.5±6.1 / 79.0±9.0 / 78.5±3.3 / 92.4±7.6 / 76.8±2.8 / 86.7±3.5 / 82.7±5.5 / 78.6±4.5
12 / 69.2±5.1 / 67.4±6.0 / 73.9±4.4 / 78.5±6.9 / 75.8±3.5 / 75.3±8.7 / 73.1±4.2 / 69.3±8.9
13 / 67.0±6.1 / 63.7±8.7 / 66.1±6.7 / 84.1±8.1 / 73.1±7.9 / 82.5±3.5 / 75.0±5.8 / 90.6±3.7
14 / 66.3±8.2 / 59.5±7.1 / 80.9±0.1 / 93.7±4.7 / 82.9±5.0 / 91.4±5.3 / 79.4±0.9 / 99.0±1.0
15 / 68.5±5.9 / 73.8±1.5 / 69.7±4.4 / 83.2±6.6 / 81.2±5.8 / 80.6±9.8 / 95.7±2.8 / 88.7±8.2
16 / 70.8±7.5 / 82.8±6.5 / 74.5±1.7 / 92.5±3.8 / 94.4±7.0 / 87.2±4.8 / 90.3±5.0 / 89.0±1.8
17 / 73.1±1.9 / 94.7±0.9 / 77.5±4.5 / 82.6±9.4 / 77.0±2.3 / 82.8±1.5 / 90.4±1.4 / 75.6±1.4
18 / 56.4±3.2 / 89.2±2.1 / 71.6±7.3 / 79.8±4.7 / 65.5±5.5 / 85.4±5.2 / 91.0±3.8 / 86.3±5.4
19 / 63.5±3.0 / 50.0±5.5 / 70.0±5.8 / 70.2±3.4 / 70.0±1.9 / 63.5±7.1 / 80.8±0.8 / 70.0±2.5
20 / 70.6±5.1 / 37.2±8.9 / 64.2±5.8 / 77.4±6.0 / 73.1±6.5 / 45.8±8.7 / 69.0±2.2 / 76.2±6.3
21 / 66.6±0.8 / 73.5±4.7 / 53.3±5.2 / 70.9±3.4 / 70.3±8.8 / 64.7±6.1 / 65.1±9.8 / 50.0±5.0

Data represent the mean ± SE (n = 10)

TableS7. Germination rates of Egyptian broomrapeseeds conditioned with water or GA3 in response to strigol at concentrations of 0.1 mg L-1 and 1 mg L-1 over 21 days of conditioning.

Conditioning period (days) / 0.1 mg/L strigol / 1 mg/L strigol
100 μM GA3 / 10 μM GA3 / 1 μM GA3 / distilled water / 100 μM GA3 / 10 μM GA3 / 1 μM GA3 / distilled water
0 / 52.0±2.2 / 52.0±2.2 / 52.0±2.2 / 52.0±2.2 / 45.6±0.6 / 45.6±0.6 / 45.6±0.6 / 45.6±0.6
1 / 86.3±1.9 / 67.5±3.1 / 59.4±4.2 / 83.5±6.0 / 82.0±5.1 / 60.5±5.9 / 76.5±5.3 / 53.8±4.3
2 / 90.0±6.1 / 76.4±8.0 / 73.9±5.2 / 86.9±6.8 / 73.6±8.4 / 77.9±1.7 / 86.6±3.8 / 72.4±6.2
3 / 86.0±4.4 / 80.3±3.2 / 78.3±9.6 / 90.4±4.8 / 70.5±4.1 / 87.1±2.9 / 81.9±6.9 / 77.3±1.7
4 / 91.7±3.7 / 81.0±2.3 / 87.3±3.0 / 94.4±1.9 / 61.1±5.8 / 78.6±3.6 / 85.1±2.9 / 84.3±4.0
5 / 93.7±2.9 / 88.2±4.4 / 83.3±6.6 / 95.3±1.1 / 76.7±7.2 / 91.4±2.1 / 80.4±4.5 / 69.5±6.9
6 / 83.5±6.5 / 89.3±2.6 / 79.7±4.7 / 92.7±3.7 / 74.3±2.5 / 87.0±5.8 / 77.8±2.5 / 72.9±7.2
7 / 91.4±3.8 / 92.1±1.6 / 74.1±3.1 / 90.1±5.7 / 87.4±4.9 / 93.1±0.7 / 71.1±6.2 / 69.0±7.6
8 / 81.9±9.6 / 84.1±4.3 / 80.4±3.9 / 92.2±7.0 / 88.1±6.6 / 91.1±7.0 / 74.7±2.5 / 69.3±1.9
9 / 74.0±8.5 / 80.0±6.7 / 83.0±2.2 / 93.8±1.6 / 91.3±3.2 / 87.3±4.0 / 78.5±4.7 / 62.0±7.2
10 / 91.8±0.3 / 73.3±0.0 / 84.0±1.9 / 89.6±3.1 / 84.0±7.4 / 77.7±5.4 / 67.3±4.1 / 65.5±2.4
11 / 78.1±1.0 / 78.5±3.6 / 87.0±1.3 / 84.2±2.7 / 71.7±4.4 / 91.5±0.4 / 69.9±1.8 / 74.6±8.3
12 / 74.9±8.3 / 74.2±2.2 / 77.3±8.7 / 82.6±6.1 / 69.9±6.4 / 66.0±5.1 / 50.6±4.4 / 73.8±4.8
13 / 86.0±4.0 / 76.8±3.3 / 70.1±5.2 / 85.5±5.3 / 75.2±6.5 / 54.0±7.9 / 57.3±3.7 / 66.4±3.5
14 / 82.1±2.4 / 73.8±9.3 / 65.6±2.5 / 90.5±6.4 / 70.8±3.4 / 55.2±9.4 / 76.0±3.4 / 80.5±3.1
15 / 80.0±3.6 / 81.3±6.1 / 80.5±4.3 / 89.0±3.3 / 67.5±7.3 / 70.5±6.2 / 82.0±3.3 / 82.2±3.0
16 / 73.0±4.1 / 66.9±3.9 / 89.1±3.9 / 96.6±2.3 / 64.6±6.7 / 78.6±6.5 / 67.3±8.0 / 75.3±3.7
17 / 85.1±3.2 / 82.7±3.2 / 80.1±6.7 / 91.0±3.1 / 66.5±1.2 / 79.8±3.2 / 67.7±3.9 / 61.6±1.9
18 / 92.2±3.1 / 82.1±2.7 / 82.3±2.9 / 95.0±0.8 / 69.3±6.1 / 79.4±7.3 / 80.3±4.8 / 76.4±3.3
19 / 93.2±1.1 / 65.5±7.7 / 77.4±10.0 / 83.4±4.3 / 60.1±5.1 / 69.8±1.7 / 68.9±3.1 / 61.5±6.2
20 / 84.5±9.4 / 63.5±7.0 / 62.0±1.1 / 69.9±4.8 / 69.3±4.1 / 67.2±2.8 / 66.0±2.1 / 59.4±7.8
21 / 88.4±7.3 / 74.7±7.1 / 51.5±5.1 / 68.6±5.0 / 62.5±7.8 / 69.5±5.2 / 43.6±5.3 / 57.4±4.0

Data represent the mean ± SE (n = 10)

TableS8. Germination rates of Egyptian broomrapeseeds conditioned with water or GA3 in response to DCL at concentrations of 0.1 mg L-1 and 1 mg L-1 over 21 days of conditioning.

Conditioning period (days) / 0.1 mg/L DCL / 1 mg/L DCL
100 μM GA3 / 10 μM GA3 / 1 μM GA3 / distilled water / 100 μM GA3 / 10 μM GA3 / 1 μM GA3 / distilled water
0 / 16.1±2.0 / 16.1±2.0 / 16.1±2.0 / 16.1±2.0 / 17.6±5.6 / 17.6±5.6 / 17.6±5.6 / 17.6±5.6
1 / 56.2±3.7 / 41.0±3.0 / 11.1±2.4 / 34.4±3.1 / 61.7±6.4 / 50.9±2.2 / 17.8±1.8 / 37.4±3.4
2 / 58.8±5.9 / 50.8±5.5 / 8.9±1.5 / 39.3±8.3 / 65.1±2.7 / 66.7±7.9 / 22.2±2.2 / 51.3±4.3
3 / 48.5±1.7 / 51.1±5.1 / 13.2±1.1 / 45.7±11.3 / 74.4±3.9 / 73.5±3.3 / 27.1±6.0 / 54.5±8.9
4 / 56.5±0.1 / 53.0±7.1 / 33.7±7.9 / 41.7±9.9 / 84.0±2.3 / 75.5±5.9 / 39.9±7.2 / 57.0±7.1
5 / 72.7±7.6 / 54.9±7.6 / 38.7±1.0 / 52.7±18.9 / 88.0±1.8 / 82.7±1.3 / 48.6±5.9 / 61.6±5.7
6 / 61.5±4.1 / 50.7±4.7 / 19.0±3.0 / 55.1±12.5 / 89.6±9.8 / 66.9±5.3 / 28.6±8.8 / 56.9±9.5
7 / 51.5±2.7 / 39.6±3.1 / 21.4±2.7 / 47.8±6.2 / 81.1±7.4 / 68.5±8.3 / 31.2±2.9 / 75.1±9.4
8 / 56.0±2.3 / 24.1±6.9 / 13.4±0.5 / 24.5±2.5 / 60.6±7.5 / 49.1±8.8 / 28.3±4.0 / 30.6±2.2
9 / 59.2±10.0 / 21.6±6.9 / 18.9±5.1 / 19.4±9.1 / 76.9±4.4 / 41.2±5.0 / 33.0±6.4 / 40.7±4.9
10 / 59.7±5.3 / 11.1±1.1 / 12.4±3.5 / 12.7±2.4 / 63.1±4.0 / 58.1±4.8 / 27.6±4.4 / 34.3±4.7
11 / 46.5±5.7 / 25.5±4.0 / 15.6±5.6 / 11.6±8.3 / 59.9±7.4 / 57.7±4.9 / 27.2±7.7 / 27.5±5.3
12 / 45.9±2.1 / 41.7±5.4 / 17.0±3.6 / 8.3±1.0 / 68.6±6.6 / 59.0±7.3 / 28.7±2.6 / 17.9±7.9
13 / 52.5±1.3 / 32.3±5.5 / 13.1±5.2 / 25.4±8.6 / 70.2±1.9 / 50.5±7.4 / 32.3±9.5 / 33.9±2.9
14 / 35.1±5.3 / 42.3±7.9 / 10.9±1.4 / 33.6±3.3 / 67.0±9.7 / 56.3±6.1 / 19.2±3.6 / 48.0±4.8
15 / 44.6±2.7 / 35.8±6.1 / 6.4±4.5 / 29.8±3.4 / 66.0±7.0 / 69.2±7.5 / 22.5±6.9 / 47.0±6.4
16 / 38.7±3.6 / 37.9±8.9 / 2.2±2.2 / 20.9±4.1 / 68.7±4.3 / 76.0±6.1 / 20.4±7.7 / 46.0±5.8
17 / 53.4±4.6 / 46.0±8.4 / 17.5±4.1 / 8.8±1.0 / 70.4±2.9 / 61.6±9.1 / 13.6±2.1 / 29.2±7.1
18 / 67.5±6.7 / 40.8±7.5 / 12.1±7.5 / 16.5±3.4 / 81.3±4.8 / 48.6±3.0 / 5.3±2.3 / 30.4±4.9
19 / 50.5±4.3 / 23.9±8.9 / 10.7±0.8 / 6.1±3.0 / 61.3±5.9 / 27.7±6.4 / 3.9±2.0 / 15.4±8.1
20 / 35.8±3.6 / 10.3±5.2 / 3.9±2.0 / 9.9±3.8 / 53.2±1.2 / 31.7±3.9 / 0.0±0.0 / 26.8±7.8
21 / 38.8±5.8 / 26.8±5.7 / 10.8±2.1 / 17.1±3.6 / 51.3±7.5 / 47.3±7.1 / 16.0±3.4 / 37.7±6.5

Data represent the mean ± SE (n = 10)

TableS9. Germination rates of Egyptian broomrapeseeds conditioned with water or GA3 in response to maize root exudates at concentrations of 1 mg L-1 and 10 mg L-1 over 21 days of conditioning.

Conditioning period (days) / 1 mg/Lmaize root exudates / 10 mg/L maize root exudates
100 μM GA3 / 10 μM GA3 / 1 μM GA3 / distilled water / 100 μM GA3 / 10 μM GA3 / 1 μM GA3 / distilled water
0 / 0.0±0.0 / 0.0±0.0 / 0.0±0.0 / 0.0±0.0 / 0.0±0.0 / 0.0±0.0 / 0.0±0.0 / 0.0±0.0
1 / 18.6±5.1 / 8.2±2.5 / 1.4±1.4 / 5.8±1.3 / 19.0±2.3 / 11.6±0.9 / 5.0±1.2 / 7.2±1.2
2 / 38.0±4.3 / 14.2±2.1 / 14.0±1.4 / 6.8±1.0 / 38.2±7.5 / 21.6±3.8 / 13.2±1.4 / 11.8±1.8
3 / 42.8±3.2 / 15.6±1.3 / 10.2±2.8 / 8.8±1.5 / 49.0±11.6 / 36.0±2.9 / 16.4±2.2 / 14.8±2.2
4 / 35.8±3.1 / 17.2±2.7 / 9.6±1.8 / 9.6±1.0 / 50.4±3.4 / 37.2±4.8 / 21.8±1.6 / 17.6±1.0
5 / 31.0±3.0 / 33.8±2.6 / 29.0±3.3 / 22.6±2.0 / 36.2±5.3 / 38.2±1.8 / 26.6±2.5 / 25.8±2.0
6 / 34.6±3.2 / 24.2±2.6 / 16.4±1.5 / 14.4±1.4 / 40.4±1.6 / 43.8±3.1 / 29.0±4.0 / 26.2±1.7
7 / 28.0±3.8 / 23.0±1.3 / 16.8±3.3 / 12.6±0.8 / 33.8±1.6 / 49.0±4.2 / 40.2±4.0 / 19.0±3.9
8 / 24.8±1.9 / 17.8±4.7 / 15.0±1.1 / 8.2±1.5 / 34.0±1.6 / 45.4±2.5 / 39.2±3.7 / 13.8±1.7
9 / 22.8±2.0 / 16.0±2.7 / 14.0±3.2 / 9.2±1.0 / 31.2±1.9 / 42.2±2.9 / 33.4±2.2 / 8.8±1.5
10 / 26.2±3.8 / 13.0±1.2 / 9.0±1.4 / 7.4±1.2 / 33.2±2.2 / 34.8±3.5 / 22.0±2.3 / 11.0±1.6
11 / 19.8±2.8 / 11.0±2.3 / 7.2±1.1 / 6.6±1.7 / 26.0±0.9 / 30.6±3.4 / 27.4±3.2 / 6.0±1.1
12 / 21.4±1.8 / 10.2±1.8 / 11.0±2.1 / 7.0±1.3 / 21.4±1.3 / 24.0±2.3 / 20.4±2.3 / 9.0±1.1
13 / 19.8±2.2 / 13.4±2.2 / 11.2±1.8 / 6.6±0.9 / 25.6±2.9 / 42.8±6.4 / 33.6±2.6 / 9.0±1.8
14 / 17.8±5.6 / 10.4±1.4 / 10.0±1.9 / 8.2±0.4 / 28.2±4.9 / 34.2±3.4 / 20.2±1.6 / 8.2±1.4
15 / 18.8±1.7 / 11.4±2.6 / 7.6±1.9 / 9.4±1.3 / 20.0±1.0 / 27.4±3.4 / 19.2±2.0 / 7.6±1.9
16 / 17.4±1.4 / 9.2±1.6 / 9.0±2.9 / 0.0±0.0 / 25.2±1.9 / 25.0±2.5 / 21.2±4.2 / 5.8±1.2
17 / 15.2±1.4 / 6.0±1.2 / 7.2±1.3 / 0.0±0.0 / 20.6±1.8 / 30.8±4.6 / 13.8±1.9 / 7.2±1.4
18 / 18.0±1.5 / 11.0±2.3 / 9.2±1.3 / 0.0±0.0 / 19.2±2.0 / 26.6±2.3 / 15.6±1.8 / 7.2±2.1
19 / 31.2±2.8 / 19.0±2.3 / 11.4±0.9 / 0.0±0.0 / 8.4±1.4 / 17.8±1.8 / 22.4±2.5 / 4.4±0.8
20 / 17.6±3.6 / 7.0±1.3 / 7.8±1.1 / 0.0±0.0 / 18.4±1.3 / 17.0±1.7 / 17.4±3.5 / 7.4±1.2
21 / 20.6±3.7 / 8.4±2.4 / 6.6±1.4 / 0.0±0.0 / 34.4±3.4 / 23.6±2.0 / 14.6±3.5 / 3.6±1.0

Data represent the mean ± SE (n = 10)

TableS10. Germination rates of Egyptian broomrapeseeds conditioned with water or GA3 in response to tobacco root exudates at concentrations of 1 mg L-1 and 10 mg L-1 over 21 days of conditioning.

Conditioning period (days) / 1 mg/L tobacco root exudates / 10 mg/L tobacco root exudates
100 μM GA3 / 10 μM GA3 / 1 μM GA3 / distilled water / 100 μM GA3 / 10 μM GA3 / 1 μM GA3 / distilled water
0 / 0.0±0.0 / 0.0±0.0 / 0.0±0.0 / 0.0±0.0 / 8.2±1.6 / 8.2±1.6 / 8.2±1.6 / 8.2±1.6
1 / 17.6±2.3 / 7.8±1.0 / 5.6±1.0 / 7.0±1.5 / 56.0±3.4 / 32.0±3.4 / 32.6±3.3 / 26.4±2.3
2 / 48.8±2.3 / 16.4±1.6 / 6.8±1.4 / 17.0±3.3 / 65.2±5.0 / 61.0±2.7 / 56.2±1.9 / 52.2±2.0
3 / 41.6±3.2 / 14.2±1.2 / 8.0±1.8 / 16.8±1.8 / 64.4±2.1 / 49.2±3.4 / 52.8±4.2 / 45.0±3.5
4 / 54.2±2.8 / 50.8±2.6 / 45.0±1.1 / 26.4±2.3 / 53.4±3.8 / 65.4±4.2 / 54.2±4.0 / 32.2±5.9
5 / 42.0±0.8 / 43.6±1.6 / 36.4±2.5 / 37.6±2.5 / 45.2±2.4 / 50.2±4.0 / 42.6±1.8 / 50.4±2.0
6 / 54.2±3.7 / 60.0±6.2 / 49.4±4.5 / 53.8±3.5 / 62.6±3.9 / 66.0±7.9 / 58.0±4.0 / 62.4±3.8
7 / 55.4±3.8 / 65.2±3.8 / 65.4±3.0 / 54.2±3.9 / 63.2±4.0 / 73.8±4.4 / 66.6±3.7 / 59.6±3.5
8 / 44.6±1.0 / 44.8±3.9 / 51.2±1.0 / 58.2±2.1 / 73.2±2.0 / 75.0±2.8 / 67.4±2.9 / 62.2±3.0
9 / 51.2±2.8 / 53.2±1.8 / 41.4±2.0 / 59.8±0.9 / 83.2±2.5 / 79.2±3.3 / 65.4±3.4 / 67.8±3.9
10 / 55.6±2.3 / 57.8±1.6 / 44.8±1.7 / 59.8±1.0 / 74.6±1.8 / 67.4±3.4 / 63.8±2.8 / 64.0±3.0
11 / 44.2±3.1 / 43.6±2.6 / 47.2±2.2 / 53.8±0.7 / 67.0±1.6 / 70.0±2.7 / 47.8±8.7 / 51.8±9.1
12 / 40.6±1.9 / 53.0±2.8 / 49.8±4.9 / 44.6±5.4 / 60.4±10.5 / 61.8±6.4 / 57.8±3.3 / 68.8±5.5
13 / 43.6±2.8 / 62.2±3.8 / 57.6±3.9 / 50.4±5.0 / 58.4±2.6 / 72.6±3.3 / 60.4±4.1 / 65.2±2.9
14 / 43.8±2.6 / 62.4±3.2 / 45.2±3.9 / 50.8±3.1 / 60.0±3.6 / 73.8±6.2 / 65.8±6.6 / 49.0±6.4
15 / 45.4±4.2 / 73.0±1.6 / 53.2±3.5 / 55.8±3.6 / 70.6±5.4 / 57.0±12.5 / 67.6±6.0 / 59.8±9.0
16 / 50.8±4.8 / 49.4±3.6 / 47.0±5.4 / 33.6±3.2 / 61.0±4.9 / 66.8±3.3 / 61.4±6.0 / 53.2±4.4
17 / 45.0±2.1 / 46.6±6.4 / 43.8±7.8 / 33.6±6.9 / 58.6±5.0 / 64.0±2.2 / 51.6±6.4 / 65.4±4.8
18 / 44.6±0.9 / 46.6±2.4 / 33.8±2.0 / 42.2±3.5 / 60.4±5.6 / 63.0±2.5 / 60.0±3.0 / 66.4±3.7
19 / 52.0±4.8 / 62.0±3.1 / 48.6±4.8 / 33.6±2.1 / 57.4±3.6 / 78.2±3.6 / 64.2±3.9 / 52.6±2.2
20 / 41.0±4.9 / 56.4±4.1 / 39.0±4.1 / 34.2±4.5 / 69.8±4.9 / 62.4±3.2 / 54.8±4.2 / 57.2±4.2
21 / 49.8±4.4 / 52.0±5.1 / 40.0±2.2 / 22.4±3.9 / 54.2±3.7 / 52.2±4.5 / 47.4±3.3 / 41.6±2.1

Data represent the mean ± SE (n = 10)

Table S11. Effect of conditioning period on the germination of sunflower broomrape

seeds conditioned with 10μM GA3under various germination stimulants.

Germination stimulants / Concentration
(mg L-1) / Regression model / R2 / F / Inflection
point
GR24 / 1 / y= 33.25 + 10.42x − 0.67x2 + 0.012x3 / 0.79 / 22.05** / 10.9/26.4
0.1 / y= 9.32 + 22.37x − 2.28x2 + 0.061x3 / 0.87 / 41.11** / 6.7/18.2
Strigol / 1 / y= 49.84 + 11.28x − 0.96x2 + 0.024x3 / 0.57 / 7.93** / 8.8/17.7
0.1 / y= 6.15 + 26.37x − 2.76x2 + 0.077x3 / 0.88 / 45.94** / 6.6/17.3
DCL / 1 / y= 27.01 + 12.81x − 1.19x2 + 0.032x3 / 0.72 / 15.21** / 7.9/17.0
0.1 / y= 4.89 + 14.21x − 1.52x2 + 0.042x3 / 0.74 / 17.25** / 6.3/17.8
Maize root extracts / 10 / y= 40.95 + 12.74x − 1.10x2 + 0.028x3 / 0.52 / 6.46** / 8.6/17.7
1 / y= 28.71 + 14.04x − 1.22x2 + 0.029x3 / 0.46 / 5.05* / 8.1/19.9
Tobacco root extracts / 10 / y= 26.20 + 16.67x − 1.25x2 + 0.021x3 / 0.79 / 22.92** / 8.5/31.0
1 / y= 12.92 + 25.48x − 2.71x2 + 0.071x3 / 0.90 / 51.24** / 6.2/19.3

* and **indicate significantcorrelations at the P < 0.05 andP < 0.01 level, respectively. The x value (number

of conditioning days) of the inflection point derived from the model is presented.

Table S12. Effect of conditioning period on the germination of sunflower broomrape

seeds conditioned with 1μM GA3 under various germination stimulants.

Germination stimulants / Concentration
(mg L-1) / Regression model / R2 / F / Inflection
point
GR24 / 1 / y= 46.02 + 10.23x − 0.98x2 + 0.026x3 / 0.66 / 11.80** / 7.4/17.7
0.1 / y= 5.86 + 17.04x − 1.95x2 + 0.058x3 / 0.73 / 16.41** / 6.0/16.4
Strigol / 1 / y= 31.83 + 12.38x − 1.24x2 + 0.034x3 / 0.63 / 9.99** / 7.1/17.2
0.1 / y= 7.65 + 15.68x − 1.62x2 + 0.044x3 / 0.71 / 14.51** / 6.6/17.9
DCL / 1 / y= 20.39 + 21.42x − 2.28x2 + 0.063x3 / 0.82 / 26.92** / 6.4/17.8
0.1 / y= 4.32 + 10.05x − 1.15x2 + 0.034x3 / 0.66 / 11.51** / 5.9/16.7
Maize root extracts / 10 / y= 37.59 + 13.83x − 1.22x2 + 0.030x3 / 0.58 / 8.26** / 8.0/19.1
1 / y= 11.69 + 19.52x − 1.92x2 + 0.048x3 / 0.81 / 24.95** / 6.8/19.8
Tobacco root extracts / 10 / y= 10.75 + 17.13x − 1.76x2 + 0.045x3 / 0.72 / 15.27** / 6.5/19.6
1 / y= 14.57 + 17.67x − 2.07x2 + 0.058x3 / 0.78 / 21.58** / 5.6/18.2

* and **indicate significantcorrelations at the P < 0.05 andP < 0.01 level, respectively. The x value (number

of conditioning days) of the inflection point derived from the model is presented.

Table S13. Effect of conditioning period on the germination of Egyptian broomrape

seeds conditioned with 10μM GA3under various germination stimulants.

Germination stimulants / Concentration
(mg L-1) / Regression model / R2 / F / Inflection
point
GR24 / 1 / y= 74.41 + 1.84x − 0.03x2− 0.005x3 / 0.347 / 3.18* / 9.5/-12.9
0.1 / y= 68.21 + 7.58x − 0.83x2 + 0.022x3 / 0.308 / 2.67 / 6.0/19.1
Strigol / 1 / y= 51.25 + 13.18x − 1.39x2 + 0.039x3 / 0.545 / 7.18** / 6.5/17.3
0.1 / y= 58.39 + 8.93x − 0.88x2 + 0.023x3 / 0.588 / 8.55** / 7.0/18.4
DCL / 1 / y= 40.95 + 8.36x − 0.78x2 + 0.018x3 / 0.304 / 2.62 / 7.2/21.6
0.1 / y= 37.91 + 1.46x − 0.20x2 + 0.005x3 / 0.129 / 0.89 / 4.3/22.8
Maize root extracts / 10 / y= 1.92 + 12.65x − 1.17x2 + 0.029x3 / 0.817 / 26.79** / 7.6/19.2
1 / y= 2.52 + 6.92x − 0.76x2 + 0.022x3 / 0.594 / 8.78** / 6.3/16.6
Tobacco root extracts / 10 / y= 19.09 + 13.71x − 1.08x2 + 0.025x3 / 0.742 / 17.26** / 9.4/19.4
1 / y= -3.90 + 14.08x − 1.02x2 + 0.023x3 / 0.79 / 22.54** / 11.1/18.4

* and **indicate significantcorrelations at the P < 0.05 andP < 0.01 level, respectively. The x value (number

of conditioning days) of the inflection point derived from the model is presented.

Table S14. Effect of conditioning period on the germination of Egyptian broomrape

seeds conditioned with 1μM GA3 under various germination stimulants.

Germination stimulants / Concentration
(mg L-1) / Regression model / R2 / F / Inflection
point
GR24 / 1 / y= 64.59 + 1.46x + 0.10x2− 0.007x3 / 0.398 / 3.97* / 14.4/-4.8
0.1 / y= 66.89 + 6.12x − 0.60x2 + 0.014x3 / 0.498 / 5.96** / 6.6/22.1
Strigol / 1 / y= 67.22 + 3.54x − 0.37x2 + 0.009x3 / 0.187 / 1.38 / 6.2/21.0
0.1 / y= 59.22 + 5.42x − 0.34x2 + 0.004x3 / 0.487 / 5.69** / 9.6/46.9
DCL / 1 / y= 14.86 + 6.99x − 0.70x2 + 0.017x3 / 0.736 / 16.72** / 6.6/20.9
0.1 / y= 11.17 + 4.56x − 0.55x2 + 0.016x3 / 0.399 / 3.99* / 5.5/17.4
Maize root extracts / 10 / y= -3.11 + 10.21x − 0.91x2 + 0.022x3 / 0.787 / 22.12** / 7.8/19.8
1 / y= 0.66 + 5.39x − 0.56x2 + 0.016x3 / 0.509 / 6.23** / 6.8/16.5
Tobacco root extracts / 10 / y= 21.60 + 10.39x − 0.79x2 + 0.018x3 / 0.663 / 11.81** / 10.1/19.0
1 / y= -7.34 + 13.57x − 0.97x2 + 0.020x3 / 0.794 / 23.18** / 10.3/22.0

* and **indicate significant correlations at the P < 0.05 andP < 0.01 level, respectively. The x value (number

of conditioning days) of the inflection point derived from the model is presented.

Supplemental figures

Figure S1.Diagram of the conditioning and germination assay procedure.

Figure S2. Germination rate of Egyptian broomrapeseeds pre-treated with 100µM of GA3 for 21d followed by distilled water.