daphnia food instructions.txt

Instructions for the feeding and propagation of Rhonda’s daphnia using Rhonda’s Daphnia Food. We estimate that the food in the small package is capable of growing/producing at least 200,000 live daphnids.

Culturing daphnia can be easy or difficult depending on how observant you are and how careful you are in following instructions. To paraphrase Joseph Campbell’s famous quotation about computers -- “Daphnia are like old testament gods; lots of rules and no mercy”.

  1. Water should be cleared -- but it may never become ‘clear’ before feeding. Yellow or brown water is the usual color of the ‘cleared water’ -- with the food filtered out by the daphnia -- and the ‘cleared’ but colored water is usually not food for the daphnia -- it is usually residue from algae cell walls, and other detritus. ‘Calibrate’ your eyeballs by noting the difference between water with particulates in it -- food for filter feeders such as daphnia -- as opposed to just colored water without suspended ‘stuff’. A ring of daphnia shells at the water’s edge and black detritus on the bottom is normal. We recommend doing a water change once per week by getting a small container of aged fish water. Pour all of the water -- daphnia and all through a fine net -- allowing the old water to go down the drain. Put the daphnia from the net into the small container of water. The solids at the bottom of the daphnia culture are bacteria colonies that convert ammonia and nitrites and generate infusoria -- transfer at least some of this detritus to the ‘new’ container with the daphnia. Hair algae is a problem -- remove it or the daphnia can be trapped in it as daphnia are naturally prickly like burrs. You will normally find hair algae clinging to the airline tubing like streamers in the wind -- pick it out and discard it. Clean the bucket or container you are using by using a washcloth to get the green slime and other coatings off the sides of the container. Use no cleaning agents -- no soap -- no chlorine.
  2. Pour the small container with the daphnia into your bucket or tank. No contaminants should be in the water as the daphnia are sensitive to pollution, chemicals, and dissolved metals. If you suspect there is copper in your water -- possibly from brand new copper pipes, etc. -- you can add a small bag of activated charcoal or even leave some on the bottom like gravel. Charcoal will soak up metals but it will become coated with slime and will eventually need to be replaced with new charcoal. We recommend using only aged fish water -- save some from you fish tank water changes. We do not use charcoal.
  3. Fill the bucket or tank to a normal level -- the depth of the water is not critical as long as it is over 5 inches or so. More water volume allows for more oxygen and more room for growth of the daphnia. An airline with a slow stream of bubbles is all that is necessary -- we recommend a piece of hard tubing or something to weigh down the airline so it stays under the water. Fast bubbling and airstones can trap air inside the daphnia shell or splash them out on the container wall. The stream of air bubbles must generate a flow of water from the bottom of the container to the surface. The water temperature is not critical. Any temperature from 55 to 75 F is optimal. The daphnia will be alive all the way down to freezing but they will be less active and have less growth at the cooler temperatures. Above 75 F they will survive with proper aeration but as the temperature is higher there is less oxygen and increased problems with bacteria or yeast blooms could consume all of the oxygen and suffocate the daphnia.
  4. Daphnia will be more ‘red’ at higher temperatures but this color is due to increased amounts of hemoglobin -- not color enhancements. We don’t claim the red color of the daphnia as a color enhancement food. The daphnia will almost always appear red when seen from above in a net. Lighting and the reflective shell may make them appear less red in your container.
  5. The amount of our daphnia food to feed depends on the density of your culture and how fast they eat the food. A large culture of ten thousand in a four gallon bucket would use about one eighth of a teaspoon every two days. You can mix the daphnia food in a small container of water before adding it to the daphnia or just mix it in the bucket or container with the daphnia. The water should be clear before feeding and the amount of cloudiness represents the food in the water. Some people use a stick with a white disk stapled to the bottom. There is an ‘X’ or other markings in black and markings on the stick to measure the depth. This is a turbidity meter and the length of the stick under water before you cannot see the ‘X’ allows you to adjust the amount of food added to get the same density or cloudiness regardless of the volume of the daphnia container.
  6. Green water is what many sources of daphnia information suggests as the food for daphnia. We have a limited amount due to our greenhouse but we have found ‘green water’ to be unreliable and easier to ‘crash’ than any daphnia culture. Use it if you have it but if not -- do not despair. Our food has spirulina powder in it and after feeding the water will be a dark shade of green. Live and fresh green algae -- ‘green water’ -- appears to not be necessary for the propagation of daphnia. In a recent week we sent out boxes of daphnia cultures totalling in excess of 70,000 daphnia that had been thriving and propagating in just one four gallon bucket for a week before shipping. They had been living and cloning with only ‘Rhonda’s Daphnia Food’ for their sustenance. Strong lighting and heaters are not necessary for the daphnia -- strong lighting will just grow more hair algae and really warm water has less oxygen dissolved in the water. The green water we send out with some of our packages is there in case you want to start culturing green water. Most people just feed it to the daphnia as a snack after the shock of shipping.
  7. Adjust the amount of food starting with just a pinch if there are just a few daphnia and pay attention to the time it takes for them to ‘clear’ the water. Two days to clear the water is a good starting point. Once you have a regular system figured out and you have a lot of daphnia -- you can measure the daphnia in your net when doing water changes and harvest/feed any amount of daphnia over what you find optimal for your container. Remember that at some point the numbers of daphnia, yeast, and bacteria in the container begin to affect the oxygen content and a ‘bloom’ of yeast or bacteria or a ‘boom’ of daphnia can quickly consume all of the oxygen and suffocate the daphnia. Overfeeding can produce excessive amounts of decomposing food and the resulting toxins of ammonia and nitrites in quantities above the ability of the natural bacteria colonies to convert/consume.
  8. Some things to know about daphnia in general -- and specifically about our ‘russian reds’ : most or all daphnia propagation is by the females producing live young -- usually all females and clones of the mother -- about 8 to 10 per week or so -- growth is quicker at higher temperatures and with higher feeding. Our ‘russian reds’ are smaller than ‘magna’ species and larger than ‘moina’ and this may be due to a kind of line breeding without line outcrossing. The ‘babies’ are similarly smaller. The original strain is said to have come from the alligator pond at the Moscow Zoo -- all the way back in the early 1960’s. Russian Reds rarely produce ‘resting eggs’ and therefore you may never see males or eggs. Other strains -- wild ones for instance -- may produce cysts/eggs under adverse conditions -- the ‘russian reds’ appear to not do this. We recommend keeping the cultures indoors as culturing outdoors will almost certainly result in mosquito larvae or even dragonfly larvae in the water and some wandering bird or insect can infect your culture with hydra. When your lousy neighbor calls the board of health on you because he is afraid that your outdoor tubs will cause him to get West Nile Virus -- you will wish you had kept your cultures indoors.
  9. The ingredients of our food is our trade secret but it does contain yeast, spirulina powder, vegetable flours, crushed flake food, and some minerals for shell growth and pH stabilization of the water. Sediments of the flours and anything that ends up on the bottom will generate bacteria and infusoria. The yeast is alive and there is food for the yeast in our food also. Be careful not to overfeed as the yeast and/or bacteria can ‘bloom’ and consume all of the oxygen.
  10. Filtration is not recommended as daphnia are filter feeders and require the food particles, live yeast, free floating algae (live or otherwise), bacteria, and infusoria that are floating in the water column. Any filter is likely to pull all of the above into the filter media leaving food for the daphnia only in the time immediately after feeding and before the water clears. The de-nitrification bacteria that are normally in the filter media are present in limited quantities -- in the water column, on the bottom, and on all surfaces in the bucket or container. Circulation of the water and aeration is all that is necessary (along with allowing some of the bottom detritus in the net with the daphnia to go into the cleaned container after each water change to allow the bacteria to continue their good work. In the spirit of ‘it takes a village to raise a child’ consider that the bacteria, infusoria, and free floating algae provide the variety of sizes of food for the variety of sizes of the daphnia from new to old.
  11. The nutritional value of crustaceans is always an area of misunderstanding. When we had a store customers would point to the high water content (over 90%) of water in frozen brine shrimp (artemia). My answer to that was to tell a story I heard about how the Eskimos survived without fresh vegetables. Everyone now knows that vitamin C and other nutrients are not available in meat or fat and lacking a constant supply of vegetables the Eskimos should have succumbed thousands of years ago to scurvy and other vitamin deficiencies. So how did the Eskimos survive without the benefits of fresh fruit and vegetables? The answer is that there is no vitamin C in fat or muscle but vitamin C and all the other required nutrients are somewhere in the animal’s body. Vitamin C is in the brain and B-12 is in the liver. If you are ever killed and eaten by a bear -- be assured that he will temporarily ignore your superb musculature and eat your liver first. The Eskimos of course have survived by a tradition of finding ways to eat the entire animal. This is the benefit of feeding your fish live food -- by eating the entire animal your fish are getting all the proteins and vitamins that science has discovered -- and perhaps some they do not yet know about. An additional benefit of feeding crustaceans to your fish is that the shells provide the calcium required for skeletal growth -- and your aquatic pets are most likely to be vertebrates.
  12. Due to the vagaries of shipping we occasionally have a customer email us asking what to do when half of the daphnia were killed in transit. Our answer is that they did not die from any disease -- never medicate -- but that the deaths were likely to be from water pollution or low oxygen -- or occasionally caused by cooking or freezing. If some portion of the shipped daphnia are dead we recommend following the setup procedure above for a water change and feed them some of the enclosed food -- a pinch or so. The dead daphnia can be transferred to the new container without a problem although you may want to make another water change in a day or two. There should always be daphnia shells in the water -- usually floating or forming a ring at the water's edge -- this ring and floating shells are not dead daphnia. I wipe off the ring with my finger and feed it to my smaller fish. The shells indicate that the daphnia are growing and an old shell is cast off and a new one formed in each growth stage -- or instar. The shells quickly decompose and become part of the ‘village’ of life in the water.
  13. Most aquarists appear to not have OCD (obsessive compulsive disorder) and eventually neglect to give proper care to their culture -- and a ‘crash’ occurs. Remember that the ‘russian reds’ are unlikely to have produced any resting eggs/cysts and they will not ‘reappear’ from hatched eggs/cysts. We recommend having at least one other ‘safety’ culture in a separate container -- fed very lightly -- to carry on the strain when the main food culture ‘crashes’. Technically only one female is required to repopulate the culture. All of our daphnia may be genetically identical to the original female(s) collected in that alligator pond in the 1960’s.
  14. As an extra credit bonus I will provide one last piece of information. The reproduction by females cloning themselves is called ‘parthenogenesis’ and it is quite rare -- usually only normal in crustaceans and rotifers. In vertebrates ‘cloning’ is usually confined to identical twins. ‘Cloning’ is a popular science fiction topic and check out this web page for some examples from the fertile minds of SF writers :
  15. Sexual reproduction is not just for the variety available in genetic mutations -- it appears to be required for most species to stay a step or two ahead of viruses -- viva la viruses! Without the slight shuffling of the proteins in our cells -- viruses would mutate to exactly match any set of identical protein structure and take us out as a species. Modern science can currently only ‘clone’ with great difficulty and perhaps only with females -- you may have heard of Dolly -- the sheep. If science progresses and some of the science fiction predictions above become true -- eventually the human species may be represented by females only -- and perhaps by the repeatedly cloned DNA of just one female. I have noticed a competitiveness between females and a certain disdain for the male of the species. I can foresee a time in the distant future when females have achieved the ultimate breakout through the ‘glass ceiling’ and eliminated the sex with the pesky excess of testosterone. Ultimately there may be just billions of female humans with a limited DNA set or if they really can’t get along -- one set of identical DNA with identical protein structures. As a male of the species I can only take solace in this bleak future by considering the probability that ‘revenge’ will eventually be exacted on the ultimate pure ‘XX’ s of the future -- by mutated ‘viruses’.