Curriculum Vitae

Personal Information

Nahid ASkari, Ph.D in Molecular Genetics, National Institute for Genetic Engineering & Biotechnology, Tehran, Iran.

Email:

Date of Birth: 26/08/1982

Place of Birth:kerman-Iran

Education

·  Ph.D. in Molecular Genetics, National Institute of Genetic Engineering & Biotechnology and Graduate University of Advanced Technology, Tehran and Kerman, Iran, 2010.

·  M.Sc. in Animal Genetics, Ramin University, Ahvaz, Iran, 2005.

·  B.Sc. in Agriculture, Shahid Bahonar University, Iran, 2000.

Phd Thesis title: Study of Human Dental Pulp Stem Cells Differentiation into Oligodendrocyte Progenitors Using Define Medium and Over Expression of Olig2 Transcription Factor

Books

1.  Mohammad Reza Mohammad Abadi, Nahid. Askari , Characterization of genetic structure using ISSR-PCR markers, Academic LAMBERT publication, 2011, (in English)

2.  Mohammad Reza Mohammad Abadi, Nahid. Askari, Biotechnology Analysis, Shahid Bahonar University, in press (in Persian)

3.  Amin Baghizadeh, Nahid. Askari, Biotechnology and its role in animal breeding, Shahid Bahonar University, in press (in Persian)

4.  Mohammad Reza Ghorbani, Nahid. Askari, Rabbit (breeding and management) Shahid Chamran University, in press (in Persian)

Projects

1-  Induction of Human third molar dental Pulp Stem Cells Differentiation into Oligodendrocyte and Transplantation in a Rat Model of Multiple Sclerosis. (National Project).

2-  Evaluation the new Mutation in H-Syndrome.

3-  Influence of Gastric Cancer on the Expression of Hepatocyte Growth Factor, Cyclooxygenase-2 and CCK-2 Gene in Gastric Epithelial Cells

4-  Influence of Colon Cancer on the Expression of Apoptosis Related Genes in Epithelial Cells.

5-  Study the mutation of P53,Bcl-2, MICAL2 genes in patient with gastric cancer in Kerman city using PCR-SSCP and Sequencing

6-  The comparison of cytotoxicity of doxorubicin anticancer drug and Colutea extract against human cancer cell lines

7-  In vitro and in vivo toxicity evaluation of iron and iron nanoparticles

8-  Cloning, expression and purification of soluble Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (sTRAIL) in bacterial expression host.

9-  Codon optimization, Expression and Evaluation of therapeutic properties of Recombinant human microplasmin

10-  Linkage analysis of microsatellite markers on chromosomes 1 and 2 in Japanese quail to identify quantitative trait loci.

11-  Study of Genetic Diversity of Raeini Cashmere Goat Based on Microsatellite Markers.

12-  Allelic Variations in Exon 2 of Caprine MHC Class II DRB3 Gene in Raeini Cashmere Goat.

13-  Evaluation of Myostatin Gene Polymorphism in Sanjabi Sheep by PCR-RFLP Method.

14- Determination of genetic polymorphism in indigenous and non- indigenous cattle in Kerman province using molecular markers

Experience

Cell culture: Growing and maintain cell lines

Gene cloning

Gene transfection methods

Different types of PCR

Isolating and purifying DNA, RNA, and protein

SDS-PAGE and Western blot

Real-time RT-qPCR

Electroporation

Stereotaxic surgery

Sciatic nerve surgery in animal model

Workshops and Techniques:

1.  PCR, Kerman Neuroscience Research center, Kerman, Iran. 2007.

2.  Real Time RT-qPCR, international center for science high technology and Environmental science, Kerman, Iran. 2008.

3.  Workshop instructor: DNA Extraction & PCR. The 11th Iranian pharmaceutical sciences conference, Kerman, Iran, 2008.

4.  Laboratory biosafety and biosecurity, international center for science high technology and Environmental science, Kerman, Iran. 2011.

5.  Primer design, Graduate University of Advanced Technology, Kerman, Iran. 20

6.  Scientific writing: strategies and techniques (1), Kerman University of medical science, Kerman, Iran, 2014.

7.  Scientific writing: strategies and techniques (2), Kerman University of medical science, Kerman, Iran, 2014.

8.  Submit a paper (1), Kerman University of medical science, Kerman, Iran, 2014.

9.  Submit a paper (2), Kerman University of medical science, 2014.

10.  Endnote, Kerman University of medical science, Kerman, Iran,2014.

11.  SPSS (1), Kerman University of medical science, Kerman, Iran, 2014.

12.  SPSS (2), Kerman University of medical science, Kerman, Iran, 2014.

13.  SPSS (3), Kerman University of medical science, Kerman, Iran, 2014.

14.  SPSS (4), Kerman University of medical science, Kerman, Iran, 2014.

15.  The 5th Royan International Summer School, Royan Institute, Tehran, Iran, 2014.

Degree in English:

IELTS: (overall: 6 – speaking: 7.5)

Journal Publications

1-  S. Darvish, S. Farajzadeh, N. Askari, M. M. Hayatbakhsh, S. shafieipour, S. Shamsi Meymandi, S. Mohammadi, M. Asadi, and M. M. Lashkarizadeh (2016). Generalized Lipoathrophy: A New Phenotype of H-Syndrome. Journal of Clinical Case Reports. 6(6): 1000826.

2-  A chromosome-wide QTL mapping on chromosome 2 to identify loci affecting live weight and carcass traits in F2 population of Japanese quail. E. Nasirifar, M. Talebi, A. Esmailizadeh, H. Moradian, S.S. Sohrabi, N. Askari. Anim. Sci., 61, 2016 (6): 290–297.

3-  Nahid Askari, Mohammad Mehdi Yaghoobi, Mehdi Shamsara, Saeed Esmaeili-Mahani (2015) Human Dental Pulp Stem Cells Differentiate into Oligodendrocyte Progenitors Using the Expression of Olig2 Transcription Factor. Cells Tissues Organs, 200(2):93-103. doi: 10.1159/000381668. IF: 2.137.

4-  Nahid Askari, Mohammad Mehdi Yaghoobi, Mehdi Shamsara, Saeed Esmaeili-Mahani,(2015) Tetracycline-Regulated Expression of Olig2 Gene In Human Dental Pulp Stem Cells Lead To Mouse Sciatic Nerve Regeneration Upon Transplantation.Neuroscience Journal. NSC-15-409. 305:197-208. doi: 10.1016. IF: 3.357.

5-  N. Askari, N. Karam-Zadeh, MM. Yaghoobi,M. Shamsara (2015). Development of human dental pulp stem cells expressing reverse tetracyclinecontrolled trans-activator by different transfection methods. Online Journal of Veterinary Research.Volume 19(2): 137-147.

6-  Genetic analysis of an F2 intercross between two strains of Japanese quail provided evidence for quantitative trait loci affecting carcass composition and internal organs (2014) H.Moradian, A. K. Esmailizadeh, S S. Sohrabi, E. Nasirifar, N. Askari, Mohammad R. Mohammadabadi & A. Baghizadeh. Mol Biol Rep :41:4455–4462.

7-  Mohammad Abadia, N. Askari, A. Baghizadeh, A.K. Esmailizadeh (2009) A directed search around caprine candidate loci provided evidence for microsatellites linkage to growth and cashmere yield in Rayini goats. Small Ruminant Research 81 (2009) 146–151. IF: 1.125.

8-  N. Askari , M.R. Mohammad Abadi, A. Baghi Zadeh, (2011)Characterization of genetic structure of Cattle, Goat and Sheep populations, using ISSR-PCR markers, Iranian journal of Biotechnology. Vol. 9, No. 3, 222-229. IF:0.536.

9-  N. Askari, A. Baghizadeh, M.R Mohamad Abadi (2010) Study of genetic diversity in four population of Rainy Cashmere goat using ISSR markers. genetics-novin. 5(2):49-56.

10- N. Askari, A. Baghizadeh, M.R Mohamad Abadi (2008) Analysis of the genetic structure of Iranian indigenous Raeni Cashmere goat populations using microsatellite markers. Biotechnology: An Indian Journal- Full Paper. Bi48642408.

11- S. Sohrabi, A. K. Esmailizadeh,, A. Baghizadeh, H. Moradian, M. R. Mohammadabadi, N. Askari and E. Nasirifar.(2012) Quantitative trait loci underlying hatching weight and growth traits in an F2 intercross between two strains of Japanese quail. Animal Production Science. IF: 1.286.

12- E. Nasirifar, M.Talebi, A. K. Esmailizadeh, H. Moradian, S. Sohrabi , N. Askari. (2016). A chromosome-wide QTL mapping on chromosome 2 to identify loci affecting live weight and carcass traits in F2 population of Japanese quail. MS.NO.CJAS.D1400113. IF: 1.183.

13- Baghizadeh A , M. Bahaaddini , M.R. Mohamadabadi, N. Askari (2009) Allelic Variations in Exon 2 of Caprine MHC Class II DRB3 Gene in Raeini Cashmere Goat . American-Eurasian J. Agric. & Environ. Sci., 6 (4): 454-459.

14- N Askari, MR Mohammad Abadi, MT Baigi Nassiri , A Baghizadeh, J Fayazi.(2008) Study of Genetic Diversity of Raeini Cashmere Goat Based on Microsatellite Markers, Agricultural Science,18(4):155-161.

15- .B Soufy, MR Mohammadabadi, K Shojaeyan, A Baghizadeh, S Ferasaty, N Askari and O Dayani (2009). Evaluation of Myostatin Gene Polymorphism in Sanjabi Sheep by PCR-RFLP Method. Livestock science. 19(1):81-90.

16- N. Askari, A. Baghizadeh(2012) Linkage disequilibrium and the mapping of Human Disease Genes. International Journal of Genetics and Genomics, 2014; 2(4): 68-76.

17- H Moradian, A K. Esmailizadeh, S S. Sohrabi, E Nasirifar, N Askari, M R Mohammadabadi, A Baghizadeh. (2014). Genetic analysis of an F2 intercross between two strains of Japanese quail provided evidence for quantitative trait loci affecting carcass composition and internal organs. Molecular biology reports. Springer. 41, (7): 4455-4462. IF: 2.024.

18- M.R Mohamad Abadi, A.Shahabi, A.R Noshari, N. Askari, (2011).Study of Genetic Diversity of Jabal Barez Red Goat Based on Microsatellite. Livestock science. 42(2):125-131.

19- A. Baghizadeh, N. Askari, A.R Amiri, (2014). Advances in plant molecular farming. agricultural and natural resources engineering organization journal.4(5):101-104.

20- M.B. Askari, N. Askari, V. Mirzaei Mahmoud Abadi, M. Mirhabibi (2014). Gamma Rays Destroy Plant Stress Resistance Genes. Greener Journals.

21- M.B. Askari, N. Askari, M. Mirhabibi , V. Mirzaei Mahmoud Abadi, (2014). Nuclear techniques in agriculture and genetics. American Journal of Bioscience.

Conferences

1-  Sh.Sherafat. MT. Torkzadeh Mahani, M, Abdolinasab, N. Askari (2016). Codon optimization, Expression and evaluation the properties of recombinant human epidermal growth factor. Second international and fourteenth Iranian Genetics Congress.

2-  Sh.Sherafat. MT. Torkzadeh Mahani, M, Abdolinasab, N. Askari (2016). Effect of an epidermal growth factor fusion protein on Mcf7 and SKM cell lines,19th national and 7th international congress of biology.

3-  M zaboli, MT. Torkzadeh Mahani, M. Mortezavi, N.Askari, (2016), Codon optimization and production of recombinant DT-Diaphorase enzyme. Second international and fourteenth Iranian Genetics Congress.

4-  M. Torkzadeh Mahani, MT. Torkzadeh Mahani, A.Riahi, N.Askari, M. Mortezavi (2016), Codon optimization and production of recombinant DT-Diaphorase enzyme 19th national and 7th international congress of biology.

5-  N. Askari, MM. Yaghoobi, M. Shamsara. (2015). Study of Human Dental Pulp Stem Cells Differentiation into Oligodendrocyte Progenitors Using over Expression of Olig2 Transcription Factor and use it in animal model of neurodegenerative diseases. 2 th Iranian Congress on progress in Tissue Engineering and regenerative Medicine.

6-  N. Askari, MM. Yaghoobi, M. Shamsara. (2014). Using Doxycycline gene induction to produce Oligodendrocyte progenitor from dental pulp stem cells as a way to treat multiple sclerosis. 11th International MS Congress.

7-  N. Askari, MM. Yaghoobi, M. Shamsara. (2014). Study of Human Dental Pulp Stem Cells Differentiation into Oligodendrocyte Progenitors Using Expression of Olig2 Transcription Factor and use it in multiple sclerosis. 11th International MS Congress.

8-  N. Askari,( 2007). The investigation of genetic variation in Raeini Cashmere goats using microsatellite markers. The 9 th Iranian Congress of Biochemistry & the 2nd International Congress of Biochemistry and Molecular Biology.

9-  N Askari, MR Mohammad Abadi, MT Baigi Nassiri , A Baghizadeh, J Fayazi.(2007) Study of Genetic Diversity of Raeini Cashmere Goat Based on Microsatellite loci , The 5th National Biotechnology Congress of Iran.

10- N. Askari, M.R. Mohamadabadi, Baghizadeh A, M. Bahaaddini. (2008).Study of Genetic Diversity of Raeini Cashmere Goat using ISSR markers. The Second National Conference on Cellular and Molecular Biology.

11- M. Bahaaddini , M.R. Mohamadabadi, Baghizadeh A , N. Askari (2008) Allelic Variations in Exon 2 of Caprine MHC Class II DRB3 Gene in Raeini Cashmere Goat using Taq1 enzyme . The Second National Conference on Cellular and Molecular Biology.

12- E. Esfandyarpour, N. Askari, M.R. Mohamadabadi, Baghizadeh A,(2008). Study of Genetic Diversity of Kermani sheep using ISSR markers.3th National Congress on Animal Science.

13- M. Bahaaddini , M.R. Mohamadabadi, Baghizadeh A , N. Askari (2008) Evaluation of GOLA DRB3 Gene Polymorphism in Raeini Cashmere Goat using PCR-RFLP Method. 3th National Congress on Animal Science.

14- N. Askari, M.R. Mohamadabadi, Baghizadeh A, (2007). Biotechnology and its role in animal breeding. National Conference on Women and Modern Science.

15- N. Askari, M.R. Mohamadabadi, Baghizadeh A, (2007).Microsatellite as PCR-based markers on DNA. National Conference on Women and Modern Science.

16- N Askari, MR Mohammad Abadi, A Baghizadeh.(2008) Study of Genetic Diversity of Jabal Barez Red Goat Based on Microsatellite loci , The first Agriculture Conference from the past to the present.

17- M. Pasandideh, A. Abbaszadeh. M.R. Mohamadabadi, H. Hatami, N. Askari. (2009). Effect of feed restriction during early life on broilers performance: effects of severity and duration, Agricultural Biotechnology Conference

18- M. Pasandideh, A. Abbaszadeh. M.R. Mohamadabadi, H. Hatami, N. Askari. (2009). Effect of Different Feed Restriction Programs On Broilers Performance In Two Strain (Arian & Ross). , Agricultural Biotechnology Conference.

19- A Shahabi, M.R Mohamad Abadi, A.R Noshari, N. Askari. (2010). Evaluation of Genetic Diversity of Jabal Barez Red Goat Based using Microsatellite markers. 11th Iranian Genetics Congress.