Article Title: Genetic characterization of invasion, hybridization, and native species decline in Celastrus spp.

Journal: Biological Invasions

Authors: David N. Zaya, Stacey A. Leicht-Young, Noel B. Pavlovic, Kevin A. Feldheim, Mary V. Ashley

Corresponding author: David N. Zaya, , Illinois Natural History Survey

Electronic Supplementary Material

Online Resource 1: Sample sizes, locations, and species identities of Celastrus spp. collections across the USA. Sample size corresponds to the putative species identity, while the number of unique genotypes applies to the species assignments determine genetically (“Genetic Assignment”). The geographic coordinates (latitude, longitude) are given under the name of the site.

Abbreviations used: NHP (National Historic Park), NRA (National Recreational Area), NMP (National Memorial Parkway), NP (National Park), NBP (National Battlefield Park), NHS (National Historic Site), NM (National Monument), FP (Forest Preserve), SP (State Park).

Location / State / Putative Species / Sample
Size / Genetic
Assignment / Unique genotypes
Weir Farm NHS
41.31°, -73.46° / CT / C. orbiculatus / 10 / C. orbiculatus / 10
Bunker Hill
42°, -87.78° / IL / C. scandens
Unknown / 3
4 / C. scandens
Hybrid / 3
2
Bur Oak Woods
41.64°, -87.8° / IL / C. orbiculatus
Unknown / 2
9 / C. orbiculatus
Hybrid / 10
1
Dan McMahon Woods
41.7°, -87.87° / IL / Unknown / 11 / C. orbiculatus / 11
Deer Grove
42.14°, -88.04° / IL / C. orbiculatus / 3 / C. orbiculatus / 2
Greene Valley FP
41.74°, -88.08° / IL / C. scandens
Unknown / 6
3 / C. scandens / 4
Location / State / Putative Species / Sample
Size / Genetic
Assignment / Unique genotypes
Harms Woods
42.06°, -87.77° / IL / C. orbiculatus / 11 / C. orbiculatus / 10
Illinois & Michigan Canal State Trail
41.42°, -88.22° / IL / C. scandens
Unknown / 6
6 / C. scandens / 11
Illinois Beach SP
42.46°, -87.81° / IL / C. scandens / 21 / C. scandens / 10
Lyons Woods
42.41°, -87.84° / IL / C. orbiculatus / 23 / C. orbiculatus / 17
Middlefork Savannah
42.26°, -87.89° / IL / Unknown / 4 / C. orbiculatus / 2
Private property / IL / C. scandens / 1 / C. scandens / 1
Ryerson Conservation Area
42.18°, -87.91° / IL / C. scandens
Unknown / 1
12 / C. scandens
C. orbiculatus
Hybrid
/ 4
2
3
Waterfall Glen
41.73°, -87.97° / IL / C. orbiculatus / 13 / C. orbiculatus
Hybrid / 11
2
Wright Woods
42.22°, -87.93° / IL / Unknown / 9 / C. orbiculatus / 2
Beemsterboer Natural Area
41.61°, -87.43° / IN / C. scandens
Unknown / 1
1 / C. scandens / 2
Clark and Pine Nature Preserve
41.62°, -87.39° / IN / C. scandens / 12 / C. scandens / 10
Location / State / Putative Species / Sample
Size / Genetic
Assignment / Unique genotypes
Indiana Dunes National Lakeshore
41.65°, -87.11° / IN / C. orbiculatus
C. scandens / 67
69 / C. orbiculatus
C. scandens / 54
58
Abraham Lincoln Birthplace NHP
37.55°, -85.73° / KY / C. scandens / 1 / C. scandens / 1
Boston Harbor Islands NRA
42.34°, -70.97° / MA / C. orbiculatus / 10 / C. orbiculatus / 9
Worcester County,
Utility right-of-way
42.4°, -71.85° / MA / C. orbiculatus
C. scandens
Intermediate / 1
2
5 / C. orbiculatus
C. scandens
Hybrid / 1
2
5
George Washington Memorial Parkway
38.98°, -77.15° / MD / C. orbiculatus / 3 / C. orbiculatus / 3
Sleeping Bear Dunes National Lakeshore (Mainland)
44.81°, -86.07° / MI / C. orbiculatus
C. scandens
Unknown / 2
23
3 / C. orbiculatus
C. scandens / 2
25
South Manitou Island
(Sleeping Bear NL)
45.01°, -86.11° / MI / C. scandens
Unknown / 9
1 / C. scandens / 9
Warren Dunes SP
41.92°, -86.59° / MI / C. orbiculatus
C. scandens / 25
26 / C. orbiculatus
C. scandens
Hybrid / 22
17
1
Pipestone NM
44.02°, -96.34° / MN / C. scandens / 15 / C. scandens / 4
Location / State / Putative Species / Sample
Size / Genetic
Assignment / Unique genotypes
Blue Ridge Parkway
35.75°, -82.17° / NC / C. orbiculatus
C. scandens
Unknown / 5
3
9 / C. orbiculatus
C. scandens
Hybrid / 8
2
6
Chimney Rock SP
35.44°, -82.25° / NC / C. scandens / 33 / C. scandens / 2
Great Smoky Mountains NP
35.51°, -83.54° / NC / C. orbiculatus
C. scandens / 10
10 / C. orbiculatus
C. scandens / 5
4
Homestead NM
40.29°, -96.83° / NE / C. scandens / 10 / C. scandens / 3
Morristown NHP
40.83°, -74.56° / NJ / C. orbiculatus / 9 / C. orbiculatus / 9
Hopewell Culture NHP
39.29°, -82.9° / OH / C. orbiculatus
C. scandens / 2
13 / C. orbiculatus
C. scandens / 2
13
Gettysburg NMP
39.84°, -77.3° / PA / C. orbiculatus
C. scandens
Unknown / 3
1
2 / C. orbiculatus
C. scandens / 3
2
Badlands NP
43.71°, -102.48° / SD / C. scandens / 10 / C. scandens / 10
Private property / SD / C. scandens / 10 / C. scandens / 9
Wind Cave NP
43.6°, -103.4° / SD / C. scandens / 10 / C. scandens / 2
Cumberland Mountain SP
35.9°, -85.03° / TN / Unknown / 2 / C. orbiculatus / 1
Location / State / Putative Species / Sample
Size / Genetic
Assignment / Unique genotypes
Sycamore Shoals SP
36.34°, -82.23° / TN / C. orbiculatus / 11 / C. orbiculatus / 11
Warriors’ Path SP
36.5°, -82.48° / TN / C. scandens
Unknown / 12
1 / C. scandens / 3
Guadalupe Mounatains NP
31.93°, -104.86° / TX / C. scandens / 10 / C. scandens / 2
Montgomery Hall Park
38.15°, -79.13° / VA / C. orbiculatus
unknown / 2
20 / C. orbiculatus
C. scandens / 2
14
Richmond NBP
37.54°, -77.41° / VA / C. orbiculatus / 10 / C. orbiculatus / 10
Private property / WI / C. scandens / 12 / C. scandens / 9
TOTALS / 654 / 475

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