CHRISTOS STATHOPOULOS, Ph.D
CHRISTOS STATHOPOULOS, Ph.D.
Assistant Professor
Department of Biology & Biochemistry
University of Houston
Curriculum Vitae (December 2002)
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ADDRESS: Department of Biology and Biochemistry
SR II, room 369
University of Houston
Houston, TX 77204-5001
Tel. (713) 743-2491
Fax (713) 743-8351
E-mail:
EDUCATION:
1/96-12/2000: Postdoctoral training with Professor Roy Curtiss III, Washington University
6/91-12/95: Ph.D. in Biological Sciences, Department of Microbiology, Division of Biological Sciences, The University of Texas at Austin, 1996.
9/85-3/90: B.S. in Biology, Department of Biology, University of Athens, Greece, 1990
POSITIONS HELD:
1/2001-present: Assistant Professor, Department of Biology and Biochemistry, University of Houston, TX
1/96-12/2000: Postdoctoral Research Associate, Department of Biology, Infectious Diseases Division, Washington University, MO
1/98-11/99: Scientific Consultant, Megan Health, Inc., MO
1/97-5/97: Lecturer, University College, Washington University, MO
9/91-12/92 and 6/93-12/95: Graduate Research Assistant, Department of Microbiology, University of Texas at Austin, TX
6/94-9/94 and 1/95-2/95: Visiting Scientist, Department of Immunology, University of Texas M.D. Anderson Cancer Center, Houston, TX
1/93-5/93: Teaching Assistant, Division of Biological Sciences, University of Texas at Austin, TX
9/92-12/92: Grader, Department of Microbiology, University of Texas at Austin, TX
9/90-12/90: Visiting Scientist, Department of Biochemistry, National Hellenic Research Foundation, Athens, Greece
9/89-1/90: Teaching Assistant, Department of Biology, University of Athens, Greece
RESEARCH EXPERIENCE:
1/96-12/2000: Research at Washington University working on bacterial pathogenesis and vaccine development.
6/94-9/94 and 1/95-2/95: Research in the Dept. Immunology, University of Texas M.D. Anderson Cancer Center, Houston.
6/91-12/95: Graduate research at the University of Texas at Austin.
9/90-12/90: Research at the Biochemical Laboratory of the Hellenic National Research Foundation, Athens.
11/88-12/89: Undergraduate research at the Biochemical Laboratory of the Evangelismos General Hospital, Athens, Greece.
TEACHING EXPERIENCE:
9/98-12/2000: Organizer of Microbial Pathogenesis and Vaccine Development Journal Club Seminar, Department of Biology, Washington University. (one hour per week)
1/97-5/97: Instructor & Lecturer in BIO 255-Understanding Biotechnology & the Genetic Revolution. University College, Washington University. (3-credit hour, undergraduate-level course; taught all 40 hours of lectures; 3 midterm exams, final exam, writing of a paper with presentation in class; Textbook: BR Glick & JJ Pasternak. "Molecular Biotechnology. Principles and Applications of Recombinant DNA". ASM Press, Washington, DC; Topics included: recombinant DNA technology, protein engineering, molecular diagnostics, vaccines, transgenic plants and animals, human gene therapy, bioethical and societal issues of biotechnology, biotechnology patents)
1/93-5/93: Teaching Assistant in BIO 302-Cellular & Molecular Biology. Instructor: Professor Chong S. Lee. Division of Biological Sciences, University of Texas at Austin.
9/92-12/92: Grader in MIC 319-General Microbiology. Instructor: Dr. Judy Edmiston. Dept. of Microbiology, University of Texas at Austin.
9/89-1/90: Teaching Assistant in Laboratory of Clinical Chemistry. Instructor: Professor Athanasios Dessypris. Dept. of Biochemistry, University of Athens, Greece.
1/90-12/90: Part-time teacher, Kentro Private High School, Athens, Greece.
AWARDS:
- National Research Service Award Traineeship, NIH, 1999-2000
- USDA Competitive Research Grant Award, project title: Genetic Analysis of Avian E. coli Virulence, role in project: co-PI (with R. Curtiss III & C. Dozois), 2000-2003
- USDA Competitive Research Grant Award, project title: Genetic Analysis of Avian E. coli Virulence, role in project: co-PI (with R. Curtiss III & C. Dozois), 1997-2000
- Professional Development Award, The University of Texas at Austin, 1994 (to present a poster at a national research meeting)
- Professional Development Award, The University of Texas at Austin, 1993 (to present a poster at a national research meeting)
- Award of Excellence and Distinction, Greek Ministry of Education, 1985 (given to students having outstanding grades in all subjects)
EDITORIAL RESPONSIBILITIES:
Ad-hoc reviewer for journals: Molecular Microbiology, Protein Science, Biotechnology & Bioengineering, Biochemica et Biophysica Acta
ACADEMIC AND OTHER PROFESSIONAL ACTIVITIES:
1/95-present: Member, American Association for the Advancement of Science
1/93-present: Member, American Society for Microbiology
6/89-present: Member, Hellenic Society for Biotechnology
SELECTED PRESENTATIONS (seminars-talks-posters):
(15) Stathopoulos, C. (1999) Secretion of virulence determinants by the general secretory pathway in Gram-negative bacterial pathogens. Department of Biology, Bioforum Seminar, Washington University. (seminar)
(14) Stathopoulos, C. (1999) Antigen delivery systems for mucosal vaccines. Department of Biology, Bioforum Seminar, Washington University. (seminar)
(13) Thanassi, D.G., E.T. Saulino, C. Stathopoulos, J. Heuser, and S.J. Hultgren. (1998). Role of PapC usher in pilus biogenesis across the outer membrane. Gordon Research Conference on "Bacterial Cell Surfaces". New London, NH. (poster)
(12) Stathopoulos, C., P.K. Brown, W.S. Bollen, D.L. Provence, and R. Curtiss III. (1997). Extracellular secretion of the hemaglutinin Tsh, an IgA protease-like protein of an avian pathogenic Escherichia coli strain. American Society for Microbiology 97th General Meeting, Miami Beach, FL. (poster)
(11) Georgiou, G., C. Stathopoulos, D. L. Stephens. (1996). Membrane topology of the protease Omptin (OmpT) of Escherichia coli K-12. Gordon Research Conference, Bacterial Cell Surfaces. New Hampshire. (poster)
(10) Stathopoulos, C., D.L. Stephens, J.N. Wygant, B.W. McIntyre, and G. Georgiou. (1996). Topological analysis of the Escherichia coli plasminogen activator OmpT. American Society for Microbiology 96th General Meeting, New Orleans, LA. (poster)
(9) Georgiou, G., D. L. Stephens, C. Stathopoulos, J. Bockhaus. (1996). Structure, function, and expression of a unique outer membrane protease in Escherichia coli. American Chemical Society, 1996 Spring Meeting, Anaheim, CA. (poster)
(8) Stathopoulos, C., M. D. Lundrigan, J. Dy, C. F. Earhart, and G. Georgiou. (1995) Export and sorting of the Escherichia coli outer membrane protease OmpT. American Society for Microbiology, 95th General Meeting, Washington, D.C. (poster)
(7) Georgiou, G., Stathopoulos, C., J. Dy, M. D. Lundrigan, and C. F. Earhart. (1995) Localization of truncated OmpT derivatives in the cell envelope of Escherichia coli K12. American Chemical Society, 1995 Spring Meeting, Anaheim, CA. (poster)
(6) Stathopoulos, C., M.D. Lundrigan, C.F. Earhart, and G. Georgiou. (1995) Outer membrane export of OmpT-PhoA hybrid proteins in Escherichia coli K12. Annual Austin Spring Meeting: In vivo and in vitro protein folding. Austin, TX. (poster)
(5) Stathopoulos, C., J. Dy, M. D. Lundrigan, C. F. Earhart, and G. Georgiou. (1994) Analysis of the export signals and sorting information within the E. coli outer membrane protease OmpT. The Texas Branch of the American Society for Microbiology, Fall Meeting, College Station, TX. (talk)
(4) Stathopoulos, C. M. Choe, H. L. Poetschke, G. Georgiou, and C. F. Earhart. (1993) Targeting of periplasmic proteins to the Escherichia coli surface. American Society for Microbiology, 93rd General Meeting, Atlanta, GA. (poster)
(3) Stathopoulos, C., G. Georgiou, and C. F. Earhart. (1993) Localization of alkaline phosphatase on the E. coli cell surface: An example of cell surface engineering. The Texas Branch of the American Society for Microbiology, Fall Meeting, Austin, TX. (talk)
(2) Stathopoulos, C., C. F. Earhart, and G. Georgiou. (1993) Characterization of Lpp-OmpA-PhoA tribrids exported to the Escherichia coli outer membrane. Hellenic Society for Biology, Annual Meeting, Florina, Greece. (poster)
(1) Earhart, C. F., J. A. Francisco, C. Stathopoulos, and G. Georgiou. (1992) Expression of ß-lactamase on the Escherichia coli surface. Gordon Research Conference. Bacterial Cell Surfaces, New Hampshire. (poster)
SELECTED PUBLICATIONS :
(13) Thanassi, D.G., C.Stathopoulos, K. Dodgon, D. Geiger, and S.J. Hultgren. 2002. Bacterial outer membrane ushers contain distinct targeting and assembly domains for pilus biogenesis. J. Bacteriol. 184:6260-6269.
(12) Stathopoulos, C., D.R. Hendrixson, D.G. Thanassi, S.J. Hultgren, J.W. St. Geme III, and R. Curtiss III. 2000. Secretion of virulence determinants by the general secretory pathway in Gram-negative pathogens: An evolving story. Microbes & Infect. 2:1061-1072.
(11) Stathopoulos, C., D.Provence,and R. Curtiss III. 1999. Characterization of the avian pathogenic Escherichia coli hemagglutinin Tsh, a member of the immunoglobulin A protease-type family of autotransporters. Infect. Immun. 67:772-781.
(10) Stathopoulos, C. 1999. Bacterial outer membrane proteins: topological analyses and biotechnological perspectives. Membr. Cell. Biol. 13:3-21.
(9) Meyer, P.N., M.R. Wilmes-Riesenberg, C. Stathopoulos, and R. Curtiss III. 1998. Virulence of a Salmonella typhimurium OmpD mutant. Infect. Immun. 66:387-390.
(8) Stathopoulos, C. 1998. Structural features, physiological roles, and biotechnological applications of the membrane proteases of the OmpT bacterial endopeptidase family: A Micro-review. Membr. Cell. Biol.12:1-8.
(7) Georgiou G., C. Stathopoulos, P. S. Daugherty, A. R. Nayak, B. L. Iverson, and R. Curtiss III. 1997. Display of heterologous proteins on the surface of microorganisms: From the screening of combinatorial libraries to live recombinant vaccines. Nature Biotechnol. 15:1-7.
(6) Hendrixson,D.R., M. L. de la Morena, C. Stathopoulos, and J.W. St. Geme III. 1997. Structural determinants of processing and secretion of the Haemophilus influenzae Hap protein. Mol. Microbiol. 26:505-518.
(5) Stathopoulos, C. 1996. An alternative topological model for Escherichia coli OmpA. Protein Sci. 5:170-173.
(4) Georgiou, G., D. L. Stephens, C. Stathopoulos, H. L. Poetschke, J. Mendenhall, and C. F. Earhart. 1996. Display of ß-lactamase on the Escherichia coli surface: Outer membrane phenotypes conferred by LppOmpA-ß-lactamase fusions. Protein Engineer. 9:239-247.
(3) Stathopoulos, C., G.Georgiou, and C. F. Earhart. 1996. Characterization of Escherichia coli expressing an Lpp'OmpA (46-159)-PhoA fusion protein localized in the outer membrane. Appl. Microbiol. Biotechnol. 45:112-119
(2) Georgiou, G., H. L. Poetschke, C. Stathopoulos, and J. A. Francisco. 1993. Practical applications of engineering Gram-negative bacterial cell surfaces. Trends Biotechnol. 11:6-10.
(1) Francisco, J. A., C. Stathopoulos, R. A. J. Warren, D. G. Kilburn, and G. Georgiou. 1993. Specific adhesion and hydrolysis of cellulose by intact Escherichia coli cells expressing surface anchoring cellulose of Cellulose Binding Domains. Bio/Technology 11:491-495.
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