Effects of pH and carbon source onSynechococcus PCC 7002 cultivation: biomass and carbohydrate production with different strategies for pH control

Carlos Eduardo de Farias Silva*, Eleonora Sforza, Alberto Bertucco

Department of Industrial Engineering DII, University of Padova, Via Marzolo 9, 35131, Padova, Italy

*Corresponding author: +39 331 4333638

Submission to Applied Biochemistry and Biotechnology

SUPPLEMENTARY INFORMATION

May, 2016

Table S1:Nutrient concentration in experiments with sodium bicarbonate as carbon source.

Nutrient
Concentration / Time (days)
0 / 1.80 / 3.71
pH
7 / 8.5 / 9 / 9.5 / 7 / 8.5 / 9 / 9.5 / 7 / 8.5 / 9 / 9.5
Ammonium
(mg L-1) / 343.04 ± 14.14 / 329.66 ± 8.80 / 322.86 ± 14.78 / 332.90 ± 6.99 / 297.38 ± 6.58 / 242.84 ± 18.24 / 290.15 ± 23.57 / 261.84 ± 25.64 / - / 161.43 ± 25.19 / 189.38 ± 25.07 / 221.87 ± 50.26
Phosphate
(mg L-1) / 21. 06 ± 1.28 / 23.54 ± 3.03 / 21.84 ± 0.66 / 22.19 ± 1.27 / 2.19 ± 0.45 / 21.15 ± 3.13 / 17.83 ± 3.23 / 7.92 ± 0.04 / - / 4.42 ± 1.81 / 4.08 ± 2.84 / 3.85 ± 1.06

Supplementary Data of Section 3.2.

Table S2: Nutrient concentration of buffered system using CO2-bicarbonate method.

Time
(days) / NutrientConcentration
Ammonium (mg L-1) / Phosphate (mg L-1)
Sodium Bicarbonate Concentration g L-1
5.5 / 7 / 11 / 22 / 30 / 5.5 / 7 / 11 / 22 / 30
0 / 353.61 ± 44.98 / 358.41 ± 12.48 / 342.82 ± 29.72 / 351.03 ± 27.82 / 359.29 ± 11.79 / 21.79 ± 0.69 / 22.76 ± 0.64 / 22.19 ± 0.32 / 22.15 ± 0.37 / 22.64 ± 0.32
1.80 / 159.81 / 191.20 ± 14.57 / 185.80 / 237.81 / 243.67 ± 6.94 / 10.89 / 14.09 ± 0.25 / 15.07 / 19.29 / 19.27 ± 0.68
3.73 / 88.36 ±0.91 / 56.32 ± 6.94 / 109.33 ± 39.58 / 123.06 ± 50.69 / 180.90 ± 12.48 / 2.19 ± 1.15 / 2.15 ± 0.07 / 1.87 ± 0.35 / 4.45 ± 1.97 / 12.73 ± 0.68
6.88 / 33.95 ± 2.63 / 11.96 ± 2.57 / 28.55 ± 9.16 / 25.46 ± 14.51 / 132.68 ± 5.13 / 2.02 ± 1.03 / 1.82 ± 0.18 / 1.62 ± 0.21 / 1.63 ± 0.01 / 4.58 ± 0.18
8.79 / 19.13 / 9.80 ± 0.76 / 18.64 / 6.38 / 94.35 ± 1.60 / 2.40 / 1.82 ± 0.18 / 1.32 / 1.57 / 2.04 ± 0.21
10.73 / 10.03 ± 3.79 / 5.29 ± 1.32 / 11.53 ± 4.50 / 15.02 ± 7.88 / 16.86 ± 0.21 / 1.67 ± 0.57 / 1.64 ± 0.07 / 1.31 ± 0.02 / 1.50 ± 0.04 / 1.64 ± 0.07

Supplementary Data of Section 3.4.