Buenaventura & Pape XXX: Supplementary material S1-2
Appendix S1. Morphological characters for phylogenetic analysis
1. Ocellar setae, size
0 regular
1 very strong
2. Vertical setae, size
0 regular
1 very strong
3. Setae on prosternum and/or posterior surface of hind coxa, colour
0 black
1 yellow or light brown
4. Presutural dorsocentrals, size
0 well differentiated
1 reduced
5. Scutellar marginal setae, number (including apicals)
0 two
1 three
2 four
6. Lateral margin of scutellum, dense patch of whitish soft setae
0 absent
1 present
7. Katepisternal setae, number
0 three
1 two
8. Tegula, colour
0 orange
1 black
9. Postalar wall, colour of anterior setae
0 black
1 white or yellow
10. Lower calypter, configuration of fringe of hair-like setae
0 not particularly developed or only on the anterior half
1 extending to or almost to posterior corner
11. Lower calypter, dorsal setae
0 absent
1 present
12. Male mid femur, ctenidium
0 present
1 absent
13. Male mid femur with ctenidium, shape of spines
0 rounded
1 flattened
14. Male hind femoral organ, setae
0 unmodified, not set in a patch
1 very closely set in a patch
15. Male hind femur, shape
0 straight
1 curved
16. Hind tibia, apical posteroventral seta
0 not differentiated
1 well differentiated
17. Tergites III and IV, dorsal length relative to the ventral length
0 equal
1 greater
18. Tergite V, shape of posteroventral margin
0 squared
1 rounded
19. Tergite V, shape of ventral margin
0 not enlarged
1 enlarged postero-ventrally
20. Tergite V, orientation of the setae on postero-lateral area in lateral view
0 directed posteriorly
1 directed ventrally
21. Sternites I and II, colour of setae
0 black
1 yellow or light brown
22. Sternite IV posteriorly, setosity
0 with no particular configuration
1 with a patch of strong setae
23. Sternite IV posteriorly, setosity pattern
0 continuous
1 interrupted in the middle
24. Sternite V, colour
0 orangish or brownish
1 blackish
25. Sternite V, antero-medial processes
0 absent
1 present
26. Inner margins of arms of sternite V, setosity pattern
0 sparse, not set in a particular configuration
1 forming a patch
27. Patch in the inner margins of arms of sternite V, shape of setosity
0 soft setae
1 strong setae
28. Male genitalic segments, size with regard to size of abdominal segments
0 gradually smaller
1 abruptly smaller
29. Syntergosternite 7+8, shape in dorsal view
0 as wide as long
1 longer than wide
30. Syntergosternite 7+8, colour
0 orangish or brownish or partially black
1 entirely black
31. Epandrium, shape in lateral view
0 as high as long
1 longer than high
2 higher than long
32. Cercal prong, colour
0 completely black
1 orange proximally and brown or dark brown distally
2 completely orange
33. Cercal base, shape
0 not particularly extended
1 extended laterally as rounded wing-like flanges
34. Cercal prong dorso-ventrally, shape
0 thick
1 flattened
35. Cercal base, patch of closely set setae
0 absent
1 present
36. Cercal apex in lateral view, shape
0 truncated
1 bluntly swollen
2 acute
3 bifurcated
4 rounded
37. Cercal prong, shape of posterior surface in lateral view
0 straight or almost straight
1 angulated
2 gently bent
3 abruptly bent
4 S-shaped
38. Cercal prong, thickness
0 gradually reduced to the apex
1 abruptly reduced in the distal half
39. Cercal prong, lateral keels
0 absent
1 present
40. Cercal prong, posterior swelling
0 absent
1 present
41. Cercal prong with posterior swelling, shape
0 distal and proximal parts equally pronounced
1 distal part becomes more pronounced
42. Cercal prong, shape of setae
0 regular size to long
1 minute
43. Cercal prong, swollen posterior process
0 absent
1 present
44. Cercal prong, saddle-shaped concavity
0 absent
1 present
45. Cercal prong, medial tooth-like projection
0 absent
1 present
46. Cercal prong, posterior spines
0 absent
1 present
47. Surstylus, shape
0 triangular
1 squared
48. Surstylus squared, proportions
0 longer than wide
1 shorter than wide
49. Surstylus, rounded basal lobe with strong setae
0 absent
1 present
50. Surstylus, shape of anterior and posterior margin
0 straight
1 slightly folded
51. Surstylus, length
0 at most half as long as cercal prong
1 almost as long as cercal prong
52. Surstylus apex, shape
0 simple
1 bifid
53. Surstylus, degree of sclerotization of postero-distal area with respect to the remaining surstylus
0 equally sclerotized
1 less sclerotized
54. Surstylus, membranous dorsal extension
0 absent
1 present
55. Postgonite, orientation
0 parallel to the body axis
1 perpendicular to the body axis
56. Pregonite, shape of posterior margin
0 straight or slightly curved
1 gently curved
2 strongly curved
57. Pregonite proximally, shape of margin
0 simple
1 folded
58. Pregonite distally, shape of margin
0 simple
1 folded
59. Pregonite, strong setae proximally
0 absent
1 present
60. Pregonite, rounded projection on the inner margin
0 absent
1 present
61. Pregonite apex, shape
0 rounded
1 acuted
62. Postgonal apodeme, shape
0 small or reduced
1 enlarged
63. Phallus, length
0 longer than pregonite
1 very short, equal to the pregonite or shorter
64. Phallus, connection between basi- and distiphallus
0 continuous
1 with a desclerotized strip
2 with a distinct hinge
65. Basiphallus at junction with distiphallus, short dorsal extension
0 absent
1 present
66. Basiphallus, dorsal hump
0 absent
1 present
67. Paraphallic apical elongated expansion with apical spines
0 absent
1 present
68. Phallic window
0 absent
1 present
69. Juxta
0 absent
1 present
70. Juxta, connection with paraphallus
0 demarcation absent
1 demarcation present
71. Juxta, length
0 short (half of the distiphallic length or shorter)
1 long (two times the distiphallic length)
2 extremely long (four times the distiphallic length or longer)
72. Juxta, shape
0 semi-squared
1 broad proximally and gradually getting narrow to the bifid apex
2 tongue-shaped
3 as a deflated balloon
4 bifurcated into two juxtal lateral plates
5 entire plate with lateral edges folded ventrally, resembling a long tube
6 globose
73. Juxta, shape of medial part
0 flat
1 dome-shaped
74. Juxta resembling a long tube, shape of basal half with regard to the distal half
0 equally broad
1 broader
75. Juxta, basal processes
0 absent
1 present
76. Juxtal lateral plates, direction of extention
0 anteriorly
1 laterally
77. Juxtal lateral plates, thickness
0 broad
1 narrow
78. Juxta bifurcated into two juxtal lateral plates, location of bifurcation
0 closer to the juxtal base
1 at about middle of juxtal length
2 closer to the juxtal apex
79. Juxta, shape of distal margin
0 simple
1 with horns
2 with spines
80. Juxtal basal horn
0 developed
1 reduced
81. Juxtal basal horn, length
0 about 7 times longer than the other horns
1 less than 5 times the length of the other horns
2 barely distinguishable
82. Juxtal basal horn, shape
0 surrounding the other horns
1 straight or curved, but never surrounding the other horns
83. Juxtal distal horns
0 separated and distinguishable
1 reduced or fused
84. Juxtal lateral plates, orientation in apical view
0 convergent
1 parallel
85. Juxta noticeably swollen in the middle
0 absent
1 present
86. Juxta, degree of sclerotization
0 completely sclerotized
1 membranous or semi-sclerotized
87. Paraphallic lateral plate
0 absent
1 present
88. Harpes
0 absent
1 present
89. Harpes, hinge between proximal and distal parts
0 absent
1 present
90. Phallotrema, shape
0 not folded
1 folded, forming more than one opening
91. Styli, number
0 one
1 three
2 two
92. Lateral styli
0 present
1 absent
93. Lateral styli
0 not collapsed
1 collapsed
94. Lateral styli, configuration
0 separated
1 fused
95. Lateral stylus, shape of base
0 simple
1 coiled or spiraling
96. Lateral stylus, stylar lateral plate
0 absent
1 present
97. Lateral stylus, stylar membranose lobe
0 absent
1 present
98. Lateral stylus, shape distally
0 not enlarged
1 strongly enlarged
99. Lateral stylus, shape
0 short and wider at the base
1 elongated and filiform
100. Lateral styli, distal configuration
0 very attached to the acrophallic rim
1 individualized
101. Capitis
0 present
1 absent
102. Capitis, shape
0 not elongated
1 elongated
103. Median process
0 present
1 absent
104. Median process, shape
0 tubular
1 lobulated
105. Vesica
0 present
1 reduced/absent
106. Vesica, demarcation with respect to the phallic tube
0 present
1 reduced
107. Vesica, size
0 small
1 large
108. Vesica, configuration
0 one plate
2 two plates
3 three plates
109. Vesica, shape in lateral view
0 thick and rounded
1 flattened dorsoventrally
2 flattened laterally
3 tubular
4 medially bulbous with two lateral plates
110. Vesica, shape of distal margin
0 rounded
1 acuted
2 squared
3 notched
4 pointed
5 hammer-shaped
111. Vesica, setae-like structures
0 absent
1 present
112. Vesica, surface
0 smooth
1 micro-spinose
113. Vesica, localization in the distiphallus
0 taking up the basal half
1 taking up the basal third or less
114. Female TVI, shape
0 entire
1 with a mid-dorsal line or narrow membranous longitudinal mid-dorsal line
2 composed of two plates
3 divided into two lateral door-like plates
115. Cephaloskeleton of 1st instar
0 not extended
1 extended
116. Cephaloskeleton of 1st instar
0 curved ventrally towards the parastomal bar.
1 curved ventrally and reachingthe parastomal bar.
Table S1. Molecular data used to reconstruct phylogenetic relationships within the Peckia clade.
Taxa / Author name / GenBank Accession numbers28S / COI / CytB / CAD4 / EF1a
Calliphora vomitoria / (Linnaeus, 1758) / JQ307776 / FJ025603 / FJ025705 / FJ025567 / JQ307782
Engelimyia inops / (Walker, 1849) / GQ409238 / GQ409324 / GQ409395 / n.a. / n.a.
Helicobia pilipleura / Lopes, 1939 / GQ409242 / GQ409329 / GQ409399 / n.a. / GQ409464
Helicobia rapax / (Walker, 1849) / GQ409243 / GQ409330 / GQ409400 / n.a. / GQ409465
Peckia (Euboettcheria) collusor / (Curran & Walley, 1934) / n.a. / GQ409344 / GQ409412 / n.a. / n.a.
Peckia (Pattonella) intermutans / (Walker, 1861) / GQ409256 / GQ409345 / GQ409413 / n.a. / GQ409475
Peckia (Peckia) chrysostoma / (Wiedemann, 1830) / GQ409255 / GQ409343 / GQ409411 / GQ409303 / n.a.
Peckia (Peckia) gulo / (Fabricius, 1805) / n.a. / n.a. / FJ025743 / n.a. / n.a.
Peckia (Peckia) uncinata / (Hall, 1933) / GQ409257 / GQ409346 / GQ409414 / GQ409304 / n.a.
Peckia (Sarcodexia) lambens / (Wiedemann, 1830) / n.a. / GQ409357 / GQ409424 / GQ409309 / n.a.
Peckia (Sarcodexia) tridentata / (Hall, 1937) / GQ409254 / GQ409342 / GQ409410 / GQ409302 / n.a.
Peckia (Squamatodes) ingens / (Walker, 1849) / JQ246643 / n.a. / n.a. / n.a. / n.a.
Peckimayia minutipenis / (Hall, 1937) / GQ409258 / GQ409347 / GQ409415 / n.a. / GQ409476
Sarcophaga (Neobellieria) bullata / Parker, 1916 / KC177829 / GQ223344 / KC177598 / n.a. / n.a.
Sarcophaga (Liopygia) crassipalpis / Macquart, 1839 / JF439572 / KP744020 / GQ409428 / n.a. / GQ409484
Sarcophaga (Parasarcophaga) taenionota / (Wiedemann, 1819) / KP744018 / KP744019 / n.a. / n.a. / n.a.
Titanogrypa luculenta / (Lopes, 1938) / GQ409287 / GQ409378 / GQ409445 / n.a. / GQ409495
Villegasia postuncinata / (Hall, 1933) / GQ409289 / GQ409381 / GQ409448 / n.a. / GQ409497
Wohlfahrtia vigil / (Walker, 1849) / n.a. / AF259516 / FJ379603 / n.a. / n.a.
Figure S1. Majority-rule (50%) consensus tree from the BI analysis of the morphological data the first and second character-coding schemes for the acrophallus. Values above and below branches correspond to analyses under the first and second character-coding schemes for the acrophallus respectively.
Figure S2. Consensus tree from the MP analysis of the morphological data using the first and second character-coding schemes for the acrophallus. Values above and below branches correspond to analyses under the first and second character-coding schemes for the acrophallus respectively.
Figure S3. Consensus tree from the MP analysis of the morphological data using the third character-coding scheme for the acrophallus.
Figure S4. Phylogenetic tree from the MP analysis of the molecular dataset.