BIOLOGY 204 ANTELOPE VALLEY COLLEGE

GENERAL MICROBIOLOGY SPRING 2008

INSTRUCTOR: Dr A. Hemsley

THEORY TEST 1 100 POINTS

STUDENT NAME: ______

STUDENT I.D. NUMBER: ______

• Perusal time = 10 minutes

• Test time = 90 minutes

• ANSWER ALL QUESTIONS

• Read ALL questions carefully during perusal time, you may write if you wish.

• Use the back of each sheet if you do not have enough writing space, but please label each answer.

• Question subheadings are NOT of equal points value.

• Points allocated for each subheading are shown at the end of the question.

GOOD LUCK!

QUESTION 1 (Total =10 points)

Match entries in the left column with answers from the lettered choices in the right column. Write your letter choice to the far left of each question in the spaces provided. Use each answer only ONCE. (1 Point per correct pair)

_____ 1. Physician who developed the first vaccine a. Lavoisier

_____ 2. Microbiologist who discovered antibiotics from soil bacteria b. Prokaryotes

_____ 3.Theory proposing that life forms could only arise from c. Edward Jenner

pre-existing life forms

_____ 4. Domain whose members contain a true nucleus d. Fungi

_____ 5. Cells that lack a nucleus e. Bacteria

_____ 6. Scientist who proved the Biogenesis theory f. Biogenesis Theory

_____ 7. Kingdom whose members have a chitin cell wall g. Dubos

_____8. Scholar who believed oxygen was needed for spontaneous h. Protists

generation

_____ 9. Domain whose members have a peptidoglycan cell wall i. Eukarya

_____ 10. Kingdom whose members are all single celled eukaryotes j. Pasteur

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QUESTION 2 (Total =10 points)

a. Name and describe at least three levels of structure found in proteins (6)

b. What type of molecule is shown below? (Hint: think of the 4 classes of biological macromolecule you have learned about) (1)

Explain why you think so. (3)

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QUESTION 3 (Total =20 points)

A.  Give a brief description of the following terms: (6)

Isotope

Biological Polymer

Anabolic reaction

B.  Aspartic acid, an amino acid, has the following chemical formula:

COOH

CH2 Atomic weight H = 1

O C = 12

NH2 C C O = 16

N = 14

H

What is the molecular weight of Aspartic acid? Show your working. (4)

What is the weight of 3 moles of Aspartic acid? Show your working. (5)

Aspartic acid is one of the components of Nutra Sweet â. How many molecules are present in 2.5 moles of NutraSweetâ? (Avogadro’s number = 6.02 x 10 23) (5)

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QUESTION 4 (Total =20 points)

Label the diagrams on this page. Provide a function for each of the structures you have labeled.

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QUESTION 5 (Total =20 points)

Fill in the blanks in the following sentences. (1 point per correct sentence)

1. One structure that bacteria can use for motility is the ______.

2. A sticky polymer attached to outside of the bacterial cell wall is the ______.

3. Appendages that bacteria use for conjugation are known as ______.

4. The cell wall of Gram positive bacteria contains small amounts of the substance known as ______.

5. The variable thickness of the bacterial cell wall gives a differential color staining reaction when the

______stain is applied .

6.  ______is a waxy lipid found in the wall of bacteria that cause tuberculosis.

7. The bacterial electron transport chain is located in the ______.

8.  Some microbes (like the Penicillium mold) are able to produce ______which can kill other microbes.

9. ______microscopy is the commonest method for viewing stained specimens.

10. ______microscopy views bacteria such as Treponema illuminated against a dark background.

11. ______microscopy uses ultraviolet light instead of visible light.

12. ______microscopy visualizes internal cellular components due to refractive differences.

13. ______uses two light beams and often computer-colorizes resultant images .

14. Two genera that form endospores are ______and ______.

15. ______lenses focus the X-rays used by the electron microscope

16. Bacteria that use simple inorganic substances and chemical energy to synthesize their organic requirements are called ______.

17. The energy producing potential of nutrient molecules is related to the number of ______atoms that they possess.

18. Sequential oxidation/reduction reactions are known as ______reactions.

19. The difference between anaerobic and aerobic respiration lies in the type of ______.

20. In the metabolic process known as ______, the terminal electron acceptor is an organic molecule.

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QUESTION 6 (Total =20 points)

A. Give a brief description of the process of RESPIRATION. In your answer be sure to include mention of the 2 separate pathways involved, and the 2 different types of respiration used by microbes and their energy yields. (8)

B. Describe 2 ways in which FERMENTATION differs from RESPIRATION? (4)

C. Describe the role of NADH and FADH2 in energy extraction processes. (4)

D. Explain why Lactobacillus grows slowly, even under ideal nutritional conditions. (4)

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