Bio Safety Risk Assessment Form

STD OPERATING PROCEDURE 5 BIO–SAFETY RISK ASSESSMENT FORM Date / /

Faculty of Health

Activity for assessment: _Biochemical assays done on blood and urine in rooms

OA209/210

1. Details of any infectious (or potentially infectious) material or specimens involved.

• Does this work involve handling material which is infectious (or may be infectious)?

YesxNo

• Does this work involve genetically manipulated organisms?YesNox

(Permit No.)

If Yes, attach the OGTR Project Approval Certificate to this form.

• Are imported biological materials used?YesNox

• Is there a current AQIS Permit for this work?YesNox

(Permit No.)

• Is there a current UREC Approval for this work?YesNox

(Permit No.)

List all animals (or animal tissue/products), micro-organisms, viruses, cell lines, primary cultures and body fluids used or stored and any material handled with risk of contagion, together with the Bio-safety Risk Group they belong to.

1. Type of work

Please indicate the type of work undertaken:

Yes No

Laboratory research or analysisx

Storage of samplesx

Collection and/or transport of samplesx

Clinic Procedures (if Yes please identify ______)

Sterilisation of equipment used in Health Care Facilitiesx

Pathological Wastex

1. Specify any Biological Monitoring of Staff or Students Involved in this Work, if required.

Eg antibody titre levels, immunisations

1. Physical Containment Facilities used for this work

What laboratories are used for this work?

____O-A 210_____

To what level of OGTR and / or Biosafety classification are these rooms assigned?

Complete PC2 conversion in the process of being implemented, but standard precautions are being followed when dealing with any biological samples.

List containment equipment used, eg biological safety cabinets (indicate room no.)

Biological safety cabinet, caps on rotors in the sealed centrifuge room OA210

______

List any specific equipment / facilities required

______

______

List personal protective equipment worn

_Gloves, Gowns, Glasses, Closed shoes ie standard precautions

______

List appropriate procedures being undertaken to handle biological material.

1. Blood and urine stored in appropriately marked fridges and freezers
2. Whiteboard listing with fridge/freezer contents
3. Double bagging when moving samples from fridges to biosafety cabinet
4. All work done in Biosafety cabinet except when samples have to read on the plate reader or centrifuged.
5. Rotors within the centrifuge have sealed covers

6. All waste disposed of in path waste bags.

Provide details of measures adopted to control aerosols, eg during blending, etc.

Samples, which have to be blended, are covered with parafilm and if possible are mixed within the Bio safety cabinet

______

List any specialist training undertaken and / or level of competency obtained.

C.Wishart – 28 yrs lab experience, registered with the Australian Institute of Medical Scientists

New users supervised by C. Wishart, but are also required to attended the Life Sciences induction course and the Biological Safety course hosted by K.Officer, QUT, H&S Advisory Services

______

Are treatment / disposal methods in accordance with AQIS guidelines for imported material and the Australian Standard, AS 2243 Part 3 Safety in Laboratories, for all other biological materials ?

YesxNo

1. Control Measures Required

Please tick box to indicate control measures to be used

AS the lab OA209/210 is not a PC2 facility we have endeavoured to minimise the potential exposure to aerosols and infection – see list procedures in place. We have used the AS/NZ standards as guidelines within the facilities that we have.

Laboratory research or analysis:X

Meet standards for AS/NZS 2243.3:2002, Safety in Laboratories, Part 3: Microbiological aspects and containment facilities. PC-2 laboratories for handling of material in Risk group 2 micro-organisms and biological safety cabinet for use where aerosols are produced during treatment of the sample.

Standard Precautions* for handling material where there is risk of contagion is required.

Storage of samples:X

Meet standards for AS/NZS 2243.3:2002, Safety in Laboratories, Part 3: Microbiological aspects and containment facilities. PC-2 laboratories for handling of material in Risk group 2. If samples are stored outside the PC-2 laboratory, double contain the samples in transport to the PC-2 facilities, for use.

Use Standard Precautions* for handling material where there is risk of contagion.

Collection and / or transport of samplesX

Meet standards for AS/NZS 2243.3:2002, Safety in Laboratories, Part 3: Microbiological aspects and containment facilities. Section 13.

Use Standard Precautions* for handling material where there is risk of contagion.

Clinic Procedures:

For any specialised clinic procedures that involve the handling of materials where there is the risk of contagion, ensure that Standard Precautions* are met, and accompany with a specific risk assessment of the specialised procedure, if required.

Sterilisation of Equipment used in Health Care Facilities:

Develop an infection control management plan for identification, risk assessment and control measures to address infection risks and control in Health Care Facilities – Low Risk scale.

Meet standards for AS/NZS 4815/2001, Office-based health care facilities not involved in complex patient procedures and processes – cleaning, disinfecting and sterilising reusable medical and surgical instruments and equipment, and maintenance of the associated environment.

Use Standard Precautions* for handling material where there is risk of contagion.

Pathological Waste:X

Waste should be disposed of in accordance with standard AS/NZS 2243.3:2002, Safety in Laboratories, Part 3: Microbiological aspects and containment facilities, Clause 9.2 and AS/NZS 3816, Management of clinical and related waste.

Removal and Disposal of Scalpel Blades from Scalpel Handles

Scalpel blades are to be removed from scalpel handles and disposed of in accordance with standard AS/NZS 3825:1998, Procedures and devices for the removal and disposal of scalpel blades from scalpel handles.

*Standard Precautions:

Work practices which require everyone to assume that all blood and body substances are a potential source of infection independent of perceived risk. This is the basic risk minimisation strategy for the handling of human blood and body fluids, secretions and excretions (excluding sweat) and for contacting non-intact skin and mucous membranes. Such precautions involve the use of safe work practices and protective barriers and the safe disposal of body substances and soiled materials. See AS/NZS 2243.3:2002 for safe practices under Standard Precautions.

Bio-safety risk assessment form

Lynette Duplock

Health & Safety Project Officer

Faculty of Health2 March 2004

1. Other Potential Bio-hazards / Procedures involving Potential Bio-hazards

Bio-safety risk assessment form

Lynette Duplock

Health & Safety Project Officer

Faculty of Health2 March 2004