Microbiology Lab #2 Quiz: Bacterial Growth Media & Cultures

Name: ______

Best way to prepare for this quiz? Read Lab Exercise #2 Instructions, Lab Report #2 Homework, & view Lab 2a PowerPoint Show, all available on the Bacterial Growth Media & Cultures Main Page of theVMC.

  1. Which all-purpose bacterial growth medium will we be using most often in lab?
  2. MacConkey’s (MAC) b. Tryptic Soy Agar (TSY)

c. Mannitol Salt Agar (MSA) d. Blood Agar (BAP)

  1. MacConkey’s Agar will only grow…
  2. halophilic bacteria b. Gram-positive bacteria

c. Gram-negative bacteria d. Staphylococcus

  1. Which type of media will you be pouring into Petri dishes?
  2. MacConkey’s (MAC) b. Tryptic Soy Agar (TSY)

c. Mannitol Salt Agar (MSA) d. Blood Agar (BAP)

  1. Next week, when you complete this lab by observing the results on your media plates, you will be examining “colony morphology.” What is colony morphology?
  2. The number of bacterial colonies growing on each plate
  3. The way in which bacteria can change, or “morph” while we observe them
  4. The shape of individual bacteria viewed through the microscope
  5. The appearance of bacterial colonies (their shape, color and surface features)
  1. What is an arm plate used for?
  2. Obtaining a sample of normal flora from the surface of the skin
  3. Introducing new bacteria to the skin of the arm
  4. Has many different uses. Is called an arm plate due to its arm-like shape
  5. Obtaining a sample from different areas of our home
  6. It is a device used for counting bacteria
  1. Where is one place that you will be obtaining anenvironmental sample from?
  2. noseb. kitchen sink c. throatd. arm e. fingertips
  1. Mannitol Salt agar (MSA) is…
  2. differential only b. selective only c. both differential and selective d. neither
  1. If I see bacterial growth on Blood Agar, and the media around those colonies has lost its color (changed from blood red to clear/transparent), this indicates:
  2. beta hemolysis b. alpha hemolysisc. gamma hemolysis d. normal flora
  1. The plates of agar that we will be inoculating with bacterial samples are sterile before we use them. How was the media made sterile prior to use?
  2. boilingb. ultra violet radiationc. autoclaved. we don’t touch it
  1. Which two types of bacterial growth plates will you be taking home with you?
  2. TSY & MAC b. TSY & MSAc. two TSY platesd. TSY & Blood Agar

This assignment is from the Virtual Microbiology Classroom on the free science education website Science Prof Online (ScienceProfOnline.com). Visit the website to find more science education resources such as lecture PowerPoints, practice test questions, review questions, science photos, and assignments such as this.