B.Sc. (Prog.) Life Science 2nd Year, Sem IV

Paper 15-LSPT 408- BIODIVERSITY-III PLANTS
Unit 5. Angiosperms-Importance of Cultivated plants:
  • Cereals (Wheat & Rice)
/ 2lectures / 1st Jan – 15th Jan
Unit 5. Angiosperms-Importance of Cultivated plants:
  • Legumes (Gram & Groundnut)
/ 2lectures / 16th Jan – 31st Jan
Unit 5. Angiosperms-Importance of Cultivated plants:
  • Spices (Black pepper & Fennel)
/ 2lectures / 1st Feb – 15th Feb
Unit 1. Introduction
  • Different types of tissue;
/ 2lectures / 16th Feb -29th Feb
Unit 1. Introduction
  • Organization of Different types of tissue into root, stem
/ 2lectures / 1st Mar – 15th Mar
Unit 1. Introduction
  • Organization of Different types of tissue into leaf
  • Concept ofstele
/ 2lectures / 16th Mar – 31st Mar
Unit 1. Introduction
  • Evolution ofstele
/ 1lectures / 1st Apr – 15th Apr

B.Sc. (Honours) Botany- Erstwhile FYUP

Name of Paper & Code / Allocation of Lectures / Month wise schedule / Tutorial/Assignment/ Presentation etc. / Reading suggestions
Paper 19: FUNCTIONAL PLANT BIOLOGY (THEORY)
Unit 8: Introduction to bioinformatics
  • Definition, Branches
  • Biological Database: Definition and classification
  • NCBI: Introduction,tools, databases,
  • database retrieval tool,
  • sequence submission tools,
  • BLAST (types)
  • Nucleotidedatabase
  • Protein database.
/ 7 lectures / 1st Jan – 15th Jan / Discussion on sources of Data in different databases /
  • Ghosh and Mallick
  • Pevsner

Unit 9: Sequence alignment
  • Introduction,
  • Concept of alignment;
  • Scoring Matrix (PAM and BLOSUM)
  • Multiple sequence alignment (Global, Local)
/ 6 lectures / 16th Jan – 31st Jan / Discussion on Scoring Matrix (PAM and BLOSUM) /
  • Ghosh and Mallick
  • Pevsner

Unit 9: Sequence alignment
  • Multiple sequence alignment (Local)
Unit 10: Molecular phylogeny
  • Introduction;
  • Representation of Phylogeny;
  • Types of Trees;
  • Methods of phylogeny.
Unit 7: Model organisms and systems biology / 9 lectures / 1st Feb – 15th Feb / Assignment: Model organisms and systems biology /
  • Ghosh and Mallick
  • Pevsner
  • Snustad and Simmons

Unit 4: Structural genomics
  • An overview;
  • Chromosomal Maps
  • Cytological, physical and Molecular maps of chromosomes;
  • Map-position based cloning of gene (Chromosomes walking and Chromosomes jumping)
  • Wholegenome sequencing (Shotgun and clone by clone approach)
  • The Human genome Project.
/ 7 lectures / 16th Feb -29th Feb / Test: Sequence alignment and Molecular phylogeny /
  • Ghosh and Mallick
  • Pevsner
  • Snustad and Simmons

Unit 4: Structural genomics
  • Wholegenome sequencing (Shotgun and clone by clone approach)
Unit 5: Functional genomics
  • Transcriptome (Array hybridization and gene chips to study transcriptome);
  • Proteome (2D-gel electrophoresis and MALDI)
  • Expressed Sequences Tags
  • Reversegenetics (site directed mutagenesis).
/ 10 lectures / 1st Mar – 15th Mar / Assignment: Various Types of Databases for Bioinformatics /
  • Ghosh and Mallick
  • Pevsner
  • Snustad and Simmons

Unit 6: Comparative genomics
  • Comparative features of prokaryotic and eukaryotic genome
  • Chloroplast and Mitochondrial genomics
Unit 2: Developmental and physiological mechanisms that protect plants against
environmental stress
  • Adaptation in plants
  • Changes in root: shoot ratio
  • Aerenchyna development
  • Osmotic adjustment
  • Compatible solute production.
/ 6 lectures / 16th Mar – 31st Mar / Test: Structural and Functional genomics /
  • Ghosh and Mallick
  • Pevsner
  • Snustad and Simmons
  • Taiz and Zeiger
  • Buchanan, Gruissem and Jones

Unit 3: Reactive oxygen species–Production and scavenging mechanisms
Revision and Discussions / 7 lectures / 1st Apr – 15th Apr / Revision and Discussions /
  • Taiz and Zeiger
  • Buchanan, Gruissem and Jones

Paper 19: FUNCTIONAL PLANT BIOLOGY (PRACTICALS)
  1. Quantitative estimation of peroxidase activity in the seedlings in the absence andpresence of salt stress.
/ 4 Practical / 1st Jan – 15th Jan
  1. Superoxide activity in seedlings in the absence and presence of salt stress
/ 4 Practical / 16th Jan – 31st Jan
  1. Zymographic analysis of peroxidase.
/ 4 Practical / 1st Feb – 15th Feb
  1. Zymographic analysis of superoxide dismutase activity
/ 4 Practical / 16th Feb -29th Feb
  1. Nucleic acid databases (NCBI, EmBL and DDBJ)
  2. Protein databases (PIR,PDB)
  3. Literature Database (PubMed)
/ 4 Practical / 1st Mar – 15th Mar
  1. Sequence Retrieval
  2. Sequence Homology
/ 2 Practical / 16th Mar – 31st Mar
  1. Construction of Phylogenetic tree using PHYLIP and
  2. Mock
/ 4 Practical / 1st Apr – 15th Apr