2014-TIBS-Supplement

Abad-Rodríguez and Díez-Revuelta

Supplementary material

Axon glycoprotein routing in nerve polarity, function and repair.

José Abad-Rodríguez and Natalia Díez-Revuelta

Membrane Biology and Axonal Repair Laboratory. Hospital Nacional de Parapléjicos (SESCAM), Finca La Peraleda s/n, E-45071 Toledo, Spain.

Corresponding author: José Abad-Rodríguez; Tel. +34 925396832; Fax: +34 925247745;

Molecule / Function / References
Clathrin / Coating of endocytic vesicles / [1-6]
COP (Coat Complex Protein) / Coating of vesicles for ER-Golgi trafficking
Dynamin / Scission from membrane of new endocytic vesicles
Synaptophysin / Regulation of SVes endocytosis
Kynesin / Anterograde motor protein (towards microtubule + end) / [7, 8]
Dynein / Retrograde motor protein (towards microtubule - end)
Synaptotagmin / Calcium sensor for exocytosis / [9, 10]
[11-16]
[17-19]
SNARE (Soluble NSF Attachment Receptor) ternary complex.
  • Sintaxin 1
  • SNAP25
  • Synaptobrevin/VAMP
/ SVes fusion for exocytosis
MUNC/STXBP (Mammalian UNCoordinated/SinTaXin Binding Proteins) / SNARE effectors in SVes priming for exocytosis.
Synapsin / Clustering of SVes for exocytosis
Complexin / Prevention of spontaneous SV fusion. Promotion of fusion upon Stag activation by Ca2+
RIM (Rab3 Interacting Molecule) / SV docking to presynaptic membrane. Fusion complex/ Ca2+ channel recruitment.
CSP (Cystein String Protein) / Control of SNARE and substrates assembly for exocytosis.
SV2 (Synaptic Vesicle protein 2) / Related to synaptic transmission? SV2A form is a co-receptor for Botulinum neurotoxin.
Rabs (A family of small-GTPase Ras super-family) / All aspects of membrane vesicle trafficking. More than 70 members. / [20-23]

Table SI. Prominent molecules involved in axon polarized transport and exo/endocytosis.

References

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2 Faini, M., et al. (2013) Vesicle coats: structure, function, and general principles of assembly. Trends in Cell Biology 23, 279-288

3 Grant, B. and Donaldson, J. (2009) Pathways and mechanisms of endocytic recycling. Nat Rev Mol Cell Biol 10, 597-608

4 Kirchhausen, T., et al. (2014) Molecular Structure, Function, and Dynamics of Clathrin-Mediated Membrane Traffic. Cold Spring Harbor Perspectives in Biology 6, a016725-a016725

5 Kwon, S.E. and Chapman, E.R. (2012) Glycosylation is dispensable for sorting of synaptotagmin 1 but is critical for targeting of SV2 and synaptophysin to recycling synaptic vesicles. J Biol Chem 287, 35658-35668

6 Saffarian, S., et al. (2009) Distinct Dynamics of Endocytic Clathrin-Coated Pits and Coated Plaques. PLoS Biol 7, e1000191

7 Hirokawa, N., et al. (2010) Molecular motors in neurons: transport mechanisms and roles in brain function, development, and disease. Neuron 68, 610-638

8 van den Berg, R. and Hoogenraad, C.C. (2012) Molecular motors in cargo trafficking and synapse assembly. Adv Exp Med Biol 970, 173-196

9 Geppert, M., et al. (1994) Synaptotagmin I: a major Ca2+ sensor for transmitter release at a central synapse. Cell 79, 717-727

10 Nishiki, T. and Augustine, G.J. (2004) Synaptotagmin I synchronizes transmitter release in mouse hippocampal neurons. J Neurosci 24, 6127-6132

11 Bloom, O. and Morgan, J. (2011) Membrane trafficking events underlying axon repair, growth, and regeneration. Mol Cell Neurosci 48, 339-348

12 Crowder, K.M., et al. (1999) Abnormal neurotransmission in mice lacking synaptic vesicle protein 2A (SV2A). Proc Natl Acad Sci USA 96, 15268-15273

13 Jahn, R. and Sudhof, T.C. (1999) Membrane fusion and exocytosis. Annual review of biochemistry 68, 863-911

14 Janz, R., et al. (1999) SV2A and SV2B function as redundant Ca2+ regulators in neurotransmitter release. Neuron 24, 1003-1016

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16 Südhof, T.C. and Rothman, J.E. (2009) Membrane fusion: grappling with SNARE and SM proteins. Science 323, 474-477

17 Liu, K.S., et al. (2011) RIM-binding protein, a central part of the active zone, is essential for neurotransmitter release. Science 334, 1565-1569

18 Kaeser, P.S., et al. (2011) RIM proteins tether Ca2+ channels to presynaptic active zones via a direct PDZ-domain interaction. Cell 144, 282-295

19 Zinsmaier, K.E. (2010) Cysteine-string protein's neuroprotective role. Journal of neurogenetics 24, 120-132

20 Lasiecka, Z.M. and Winckler, B. (2011) Mechanisms of polarized membrane trafficking in neurons -- focusing in on endosomes. Mol Cell Neurosci 48, 278-287

21 Lazo, O.M., et al. (2013) BDNF regulates Rab11-mediated recycling endosome dynamics to induce dendritic branching. J Neurosci 33, 6112-6122

22 Ng, E.L. and Tang, B.L. (2008) Rab GTPases and their roles in brain neurons and glia. Brain research reviews 58, 236-246

23 Villarroel-Campos, D., et al. (2014) Rab-mediated trafficking role in neurite formation. J Neurochem 129, 240-248

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