MS/MS peptide sequences / Sequence coverage
(%) / Mowse score
1 / gi|124802200 / QF122 antigen / 13.20 / 9.2 / 4 / 3 / 207
2 / gi|124506063 / 60S ribosomal subunit protein L4/L1, putative / 46.52 / 10.5 / 3 / 10 / 176
gi|124802670 / ribosomal protein L3, putative / 44.65 / 10.2 / 3 / 7 / 140
3 / gi|124808771 / Ribosomal protein family L5, putative / 34.21 / 9.8 / 3 / 10 / 154
gi|124512500 / hypothetical protein, conserved / 27.30 / 10.6 / 3 / 12 / 107
gi|124808774 / ribosomal protein L7a, putative / 32.85 / 10.2 / 2 / 8 / 101
4 / gi|124505117 / 40S ribosomal protein S3A, putative / 30.43 / 9.8 / 2 / 7 / 74
5 / gi|124804772 / Ribosomal protein / 16.37 / 10.6 / 2 / 14 / 57
6 / gi|124511652 / hypothetical protein PF07_0007 / 14.24 / 9.6 / 3 / 27 / 168
gi|124504853 / 60S Acidic ribosomal protein P2 / 12.06 / 4.5 / 2 / 36 / 151
gi|2062367 / histone H4 / 9.15 / 11.0 / 2 / 27 / 102
Table S4 – Plasmodium falciparum proteins from iRBCs membrane extracts detected out of the master gel scope after metabolic labeling and identified by LC Q-TOF MS/MS.
The proteins were identified by LC Q-TOF MS/MS following in-gel trypsin digestion. The spots representing the identified proteins are indicated in Fig.2. The theoretical MW and pI values, as well as the number of matching peptides and the protein amino acid sequence coverage by the matching peptides are given. Mowse score serves as an indicator of confidence of identification (Protein scores greater than 40 are significant (p<0.05)).
2