Appendix A. Supplementary material

Table S1. PCR primers and thermal cycling conditions used for quantification of the total number of bacterial 16S rRNA gene copies and those from specific taxa

Primers / Sequence (5’-3’) / Thermal conditions
16S rRNA:
341F
534R / CCTACGGGAGGCAGCAG
ATTACCGCGGCTGCTGGCA / 95C, 15 min, 1 cycle
95C for 15 s, 60C for 30 s, 72C for 30 s, 80C for 30 s, 35 cycles
95C for 15 s, 80C to 95C, 1 cycle
Data acquisition at: 80C
Alphaproteobacteria:
Eub338
Alf685 / ACTCCTACGGGAGGCAGCAG
TCTACGRATTTCACCYCTAC / 95C, 15 min, 1 cycle
95C for 15 s, 60C for 30 s, 72C for 30 s, 80C for 30 s, 35 cycles
95C for 15 s, 80C to 95C, 1 cycle
Data acquisition at: 80C
Actinobacteria:
Actino235
Eub518 / CGCGGCCTATCAGCTTGTTG
ATTACCGCGGCTGCTGG / 95C, 15 min, 1 cycle
95C for 15 s, 60C for 30 s, 72C for 30 s, 80C for 30 s, 35 cycles
95C for 15 s, 80C to 95C, 1 cycle
Data acquisition at: 72C
Firmicutes:
Lgc353
Eub518 / GCAGTAGGGAATCTTCCG
ATTACCGCGGCTGCTGG / 95C, 15 min, 1 cycle
95C for 15 s, 55C for 30 s, 72C for 30 s, 80C for 30 s, 35 cycles
95C for 15 s, 80C to 95C, 1 cycle
Data acquisition at: 80C
Bacteriodetes:
Cbf319
Eub518 / GTACTGAGACACGGACCA
ATTACCGCGGCTGCTGG / 95C, 15 min, 1 cycle
95C for 15 s, 60C for 30 s, 72C for 30 s, 80C for 30 s, 35 cycles
95C for 15 s, 80C to 95C, 1 cycle
Data acquisition at: 81C
Acidobacteria:
Acid31
Eub518 / GATCCTGGCTCAGAATC
ATTACCGCGGCTGCTGG / 95C, 15 min, 1 cycle
95C for 15 s, 55C for 30 s, 72C for 30 s, 80C for 30 s, 35 cycles
95C for 15 s, 80C to 95C, 1 cycle
Data acquisition at: 80C
Gemmatimonadetes:
Gemmo440F
534R / TTCGGRKTGTAAACCACTGT
ATTACCGCGGCTGCTGGCA / 95C, 15 min, 1 cycle
95C for 15 s, 55C for 30 s, 72C for 30 s, 80C for 30 s, 35 cycles
95C for 15 s, 80C to 95C, 1 cycle
Data acquisition at: 72C
Verrucomicrobia:
Verru349
Eub518 / GYGGCASCAGKCGMGAAW
ATTACCGCGGCTGCTGG / 95C, 15 min, 1 cycle
95C for 15 s, 55C for 30 s, 72C for 30 s, 80C for 30 s, 35 cycles
95C for 15 s, 80C to 95C, 1 cycle
Data acquisition at: 82C

Figure S1. Overview of the Ultuna long-term soil organic matter experiment. The shaded treatments (B = Control; F = Straw; G = Straw + N; I = Peat; M = Peat + N) in blocks I-III were sampled and used in this study. Block IV was not sampled because it is not randomized.