Appendix A. Number of samples where metals were detected or not detected for each species examined.

Tissue / Metal / Species / Detects / Non-detects / %Non-detects
Fillet / As / Bluegill / 205 / 134 / 40
Redear / 165 / 13 / 7
Largemouth Bass / 317 / 25 / 7
Hg / Bluegill / 321 / 18 / 5
Redear / 177 / 1 / 1
Largemouth Bass / 342 / 0 / 0
Se / Bluegill / 337 / 2 / 1
Redear / 178 / 0 / 0
Largemouth Bass / 342 / 0 / 0
Ovary / As / Bluegill / 54 / 12 / 18
Redear / 65 / 2 / 3
Largemouth Bass / 101 / 1 / 1
Hg / Bluegill / 12 / 54 / 82
Redear / 11 / 61 / 85
Largemouth Bass / 35 / 67 / 66
Se / Bluegill / 66 / 0 / 0
Redear / 67 / 0 / 0
Largemouth Bass / 102 / 0 / 0
Liver / As / Bluegill / 16 / 4 / 20
Redear / 41 / 0 / 0
Largemouth Bass / 102 / 0 / 0
Hg / Bluegill / 18 / 2 / 10
Redear / 41 / 0 / 0
Largemouth Bass / 100 / 2 / 2
Se / Bluegill / 20 / 0 / 0
Redear / 41 / 0 / 0
Largemouth Bass / 102 / 0 / 0

Appendix C. Correlations between organ metal concentrations.

In general, fillet metal concentrations were related to those in other tissues (ovary and liver), especially those in the ovary where all relationships between fillet and ovary tissue were significant at the α=0.05 level (Appendix B). However, there was a significant negative relationship between redear sunfish fillet Hg and ovary Hg. No significant relationships were found for the correlation between fillet and ovary As in bluegill, and fillet/ovary Hg in largemouth bass. Also, there were three correlations between liver and fillet— two in bluegill (As and Hg) and one in redear sunfish (Hg)—that were not significant, but all other correlations were significant even after a Bonferroni correction was applied. Despite these caveats, it was concluded that fillet concentrations can reasonably predict liver and ovary concentrations.

Table C1. Significant (α=0.05) Pearson’s correlation coefficients (top number) and degrees of freedom (bottom number) for correlations between fillet (F) and liver (L) and fillet and ovary (O) arsenic (As), mercury (Hg) and selenium (Se) bioaccumulation. Gray shading highlights boxes with negative correlations. Empty boxes indicate non-significant correlations.

L As / L Hg / L Se / O As / O Hg / O Se
Bluegill F As / 0.30
50
F Hg / 0.65*
56
F Se / 0.67*
15 / 0.72*
56
Redear F As / 0.62*
36 / 0.74*
66
F Hg / -0.29
66
F Se / 0.73*
36 / 0.82*
66
Largemouth F As
Bass / 0.65*
102 / 0.83*
102
F Hg / 0.80*
102 / 0.27
102
F Se / 0.62*
102 / 0.64*
102

*P< Bonferroni corrected αnew= 0.002 for 18 comparisons

Appendix D. Time-series of mean blood chemistry concentrations by site and year for each species examined. Legend is the same for all parts of the figure.

Appendix D continued

Appendix D continuedAppendix D continued

Appendix E. Results of Tukey’s post-hoc tests (completed following the MANCOVA shown in Table 4) comparing blood chemistry functional response groups least square means (LS Means and the SE of the LS Means) at reference and spill sites (P-value Diff LS Means).

Species / Dependent Variable / Site Type / LS Means / SE / P-value
Diff LS Means
Bluegill / Bioenergetics / Reference / 53.3246 / 5.7580 / 1.0000
Spill / 50.5681 / 4.8065
Carbo-prot metab / Reference / 32.4668 / 3.1710 / 1.0000
Spill / 33.0945 / 2.5641
Electrolyte balance / Reference / 58.4084 / 3.5563 / 1.0000
Spill / 57.9573 / 2.9057
Hematology / Reference / 16.5927 / 4.3441 / 1.0000
Spill / 15.1373 / 3.4781
Organ function / Reference / 14.7824 / 3.1803 / 1.0000
Spill / 12.5591 / 2.5648
Redear / Bioenergetics / Reference / 85.5039 / 5.4487 / 0.9959
Spill / 78.6867 / 4.7794
Carbo-prot metab / Reference / 34.3818 / 2.9199 / 1.0000
Spill / 35.6306 / 2.5418
Electrolyte balance / Reference / 58.5674 / 3.2981 / 1.0000
Spill / 58.9723 / 2.8777
Hematology / Reference / 17.6014 / 4.1735 / 1.0000
Spill / 19.2581 / 3.7480
Organ function / Reference / 13.6632 / 2.9335 / 1.0000
Spill / 13.3155 / 2.5458
Largemouth Bass / Bioenergetics / Reference / 210.63 / 5.3774 / 0.9999
Spill / 214.77 / 4.8588
Carbo-prot metab / Reference / 38.3231 / 2.7720 / 0.9734
Spill / 42.9354 / 2.5019
Electrolyte balance / Reference / 58.3976 / 3.1725 / 1.0000
Spill / 57.6765 / 2.8682
Hematology / Reference / 17.6141 / 3.9137 / 1.0000
Spill / 17.6068 / 3.4743
Organ function / Reference / 17.39126 / 2.7747 / 1.0000
Spill / 16.0574 / 2.5005
Species / Dependent Variable / Site Type / LS Means / SE / P-value
Diff LS Means
Bluegill / Condition factor / Reference / 1.6545 / 0.0093 / 0.9813
Spill / 1.6400 / 0.0076
Gonadosomatic / Reference / 0.0772 / 0.0093 / 1.0000
Spill / 0.0720 / 0.0076
Hepatosomatic / Reference / 0.0127 / 0.0093 / 1.0000
Spill / 0.0196 / 0.0076
Splenosomatic / Reference / -0.0041 / 0.0093 / 1.0000
Spill / 0.0050 / 0.0076
Viscerosomatic / Reference / 0.0291 / 0.0093 / 1.0000
Spill / 0.0390 / 0.0076
Redear / Condition factor / Reference / 1.6380 / 0.0097 / 0.0450
Spill / 1.5941 / 0.0085
Gonadosomatic / Reference / 0.0849 / 0.0097 / 0.9544
Spill / 0.0672 / 0.0085
Hepatosomatic / Reference / 0.0198 / 0.0097 / 0.9993
Spill / 0.0097 / 0.0085
Splenosomatic / Reference / 0.0058 / 0.0097 / 0.9995
Spill / -0.0039 / 0.0085
Viscerosomatic / Reference / 0.0348 / 0.0097 / 0.9998
Spill / 0.0262 / 0.0085
Largemouth Bass / Condition factor / Reference / 1.4407 / 0.0134 / 0.0939
Spill / 1.4942 / 0.0117
Gonadosomatic / Reference / 0.0559 / 0.0134 / 1.0000
Spill / 0.0577 / 0.0117
Hepatosomatic / Reference / 0.0147 / 0.0134 / 1.0000
Spill / 0.0137 / 0.0117
Splenosomatic / Reference / 0.0022 / 0.0134 / 1.0000
Spill / 0.0004 / 0.0117
Viscerosomatic / Reference / 0.0419 / 0.0134 / 1.0000
Spill / 0.0410 / 0.0117

Appendix F. Results of Tukey’s post-hoc tests (completed following the MANCOVA shown in Table 5) comparing condition factor least square means (LS Means and the SE of the LS Means) at reference and spill sites (P-value Diff LS Means). Significant P-values (α=0.05) are shown in bold.

Appendix G. Results of Tukey’s post-hoc tests (completed following the MANCOVA shown in Table 6) comparing reproductive metric least square means (LS Means and the SE of the LS Means) at reference and spill sites (P-value Diff LS Means).

Species / Dependent Variable / Site Type / LS Means / SE / P-value
Diff LS Means
Bluegill / Atretic oocytes / Reference / -403.16 / 323.33 / 0.7091
Spill / 212.99 / 231.78
Batch fecundity / Reference / 5473.83 / 323.33 / 1.0000
Spill / 5485.11 / 231.78
Vitellogenic oocytes / Reference / 8137.37 / 323.33 / 0.7395
Spill / 7542.77 / 231.78
Redear / Atretic oocytes / Reference / 1249.03 / 808.81 / 0.5164
Spill / -606.11 / 589.12
Batch fecundity / Reference / 13835 / 808.81 / 0.4288
Spill / 11835 / 589.12
Vitellogenic oocytes / Reference / 17694 / 808.81 / 0.0694
Spill / 14740 / 589.12
Largemouth Bass / Atretic oocytes / Reference / -790.91 / 4819.34 / 1.0000
Spill / -242.99 / 3730.11
Batch fecundity / Reference / 26446 / 4922.49 / 1.0000
Spill / 25845 / 3756.04
Vitellogenic oocytes / Reference / 74638 / 4819.34 / 0.9998
Spill / 72922 / 3730.11