Yeast strain YM4271

YPD media (2% Difco peptone, 1% yeast extract, 2% dextrose)

Hazardous Substances Policy - Assessment

CHEMICAL HAZARD AND RISK ASSESSMENT

School of Biosciences

Name of supervisor / Dr S. Minchin / Assessment Number* / 1838
Signature
Assessor / Rita Godfrey / Date of Assessment / 2 / 8 / 01

Signature

Notes A School COSHH form in Word is available on the School Server.

Available from the Health and Safety Unit.

Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.

Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.

Use a continuation sheet to expand any section of this form in hard copy version.

1 / LOCATION OF THE WORK ACTIVITY / Lab G10
2 / PERSONS WHO MAY BE AT RISK
List names where possible / All occupants of the lab ( Rita Godfrey, Jo Field, Dong Ling Zeng)
3 / ACTIVITY ASSESSED / Yeast Transformations
4 / MATERIALS INVOLVED / Attach copies of data sheet(s)
NAME / AMOUNT** / HAZARD / RISK PHRASES / HAZDAT NO*** / BIOSCIENCESNO***
Yeast strain YM4271 / 1 colony – 300ml / Hazard Group 1 GMAG approved
YPD media (2% Difco peptone, 1% yeast extract, 2% dextrose) / up to 300ml / No data available. See SD agar below
10 x TE (100mM Tris, 10mM EDTA pH7.5) / 40ml / NSH
1 x TE / 150ml / NSH
Lithium acetate / 4.08g / TOXIC / R23/25- Toxic by inhalation and if swallowed / 1192
10 x LiAc (1M) / 40ml / HARMFUL / R20/22- Harmful by inhalation and if swallowed
1 x TE/LiAc / 2ml / NSH
Plasmid DNA / 1-20ug per sample / Hazard Group 1 GMAG approved
Herring testes carrier DNA / 100ug per sample / No data available but probably NSH
PEG 4000 / 20g / NSH
50% PEG / 40ml / NSH
PEG/LiAc (40% PEG, 1 x TE, 1 x LiAc) / 0.6-10ml / NSH
DMSO / up to 1.05ml / HARMFUL / R22- Harmful if swallowed
R36/38 - Irritating to eyes and skin / 1190
SD agar plates + supplements (0.67% yeast nitrogen base without amino acids, 2% agar, supplemented with amino acid solution minus the one you are selecting for) / 2-30 / No hazard data available but avoid inhaling dust as a precaution, particularly if asthmatic
5 / INTENDED USE
Give brief details and attach protocol/instructions / See attached sheet
6 / RISKS to HEALTH and SAFETY from INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding mothers especially at risk?
The risks to health and safety are from weighing out lithium acetate and using the 10 x solution and also using DMSO
Since no data is available for the media it is not clear what if any risks there are.
7 / CONCLUSIONS ABOUT RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
There are a few control measures necessary to minimise the risks involved.
8 / CONTROL MEASURES
Additional to Good Chemical Practice
Weigh out the lithium acetate in a fume hood.
Wear gloves when handling DMSO
As a precaution avoid creating dust when weighing out the media and if you are an asthma suffer you should certainly avoid inhaling any dust.
9 / INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
10 / MONITORING
Performance of control measures,
Check fume hood is working before use. Check integrity of gloves
Personal exposure / Health Surveillance
11 / WASTE DISPOSAL PROCEDURE
See School Server for Approved Procedure Document on specific Chemical Waste Disposal.
Any waste can be safely disposed of down the lab sink with lots of running water
Waste agar plates containing yeast colonies have to be autoclaved first.
12 / REVIEW
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
August 2006
13 / EMERGENCY ACTION
TO CONTROL HAZARDS / To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc.
Use spill kit to control the spread of DMSO or paper towels if only small.
TO PROTECT PERSONNEL / Evacuation, protection for personnel involved in clean-up, Special First Aid
Wear protective clothing and gloves when cleaning up spillages.
TO RENDER SITE OF EMERGENCY SAFE / Clean-up/decontamination
Avoid creating a dust when cleaning up spills of lithium acetate. Wash site with water
CONTACT PHONE

10.10.00

Prefix T is used for Teaching Assessment Number.

** List the amount by weight of the substance used. (for liquids eg; 100 mls 1M Sodium Hydroxide =

4g).

*** Hazdat No is the UNICOSHH datasheet report number.

Biosciences No is the Biosciences data sheet number.

UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.

BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.