The Ghrelin Mrna Expression in Chinese Perch: Response to Fasting Temperature and Photoperiod

Legends to supplementary file

File 1. Molecular characterization of Chinese perch preproghrelin.

(A) Amino acid sequence alignment of Chinese perch and other vertebrate preproghrelin precursors. The mature preproghrelin peptides are boxed. The conserved amino acid residues are shaded in black.

(B) NJ phylogenetic tree showing the evolutionary relationships of the deduced amino acid sequence of Chinese perch preproghrelin with those of other species. Scale bar indicates the substitution rate per residue. The numbers at tree nodes refer to percentage bootstrap after 1,000 replicates. The Chinese perch preproghrelin is noted with “◆”. GenBank accession numbers of the sequences used are as follows: human, AB029434; monkey, NM‐001032903; rat, AB029433; mouse, AB060078; chicken, B058510; turkey, AY333783.1; turtle, (-1, AB161457; -2, AB164158); bullfrog, AB058510; African clawed frog, AB669180; Atlantic salmon (-1, AB443431; -2, AB443432); rainbow trout, (-1, AB096919; -2, AB101443); Japanese eel, AB062427; Nile tilapia, AB104860; orange-spotted grouper, DQ343147; channel catfish, (-1, AB196449; -2, AB196450); zebrafish, EU908735; common carp, BAF95542; grass carp, JQ068139; goldfish, AF454389; sea bream, AY643808; sea bass, DQ665912; Mozambique tilapia, AB077764; largemouth bass, FJ392504; hammerhead shark, AB254130; blacktip reef shark, AB254129; horse, NM001163967; goat, GU014694; goose, AY338465; Pacific Bluefin tuna, BAM34456; Hamadryas baboon, NP001028075; cattle, NP776492.

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