Yeast Media
YPDA Liquid Media (1l)
Add ~500ml MilliQ water to a 1l bottle
20g Glucose (dissolve with stirrer before adding anything else)
20g Peptone (dissolve)
10g Yeast Extract (dissolve)
100mg adenine (won’t dissolve until autoclaved)
Make up to 1l with water and autoclave.
Store at room temperature.
YPDA Plates (1l)
Add ~500ml MilliQ water to a 1l bottle
20g Glucose (dissolve with stirrer before adding anything else)
20g Peptone (dissolve)
10g Yeast Extract (dissolve)
100mg adenine (won’t dissolve until autoclaved)
18g Agar
Make up to 1l with water and autoclave.
Pour plates in a lamina flow cabinet and allow to dry for 1 hour before storing.
Store at room temperature in the dark.
SD-leu Liquid media (1l)
Add ~500ml MilliQ water to a 1l bottle
26.7g Minimal SD base (dissolve with a stirrer before adding anything else)
0.69g –Leu DO Supplement
Make up to 1l with water and autoclave.
Store at room temperature.
SD-leu Plates (1l)
Add ~500ml MilliQ water to a 1l bottle
26.7g Minimal SD base (dissolve with a stirrer before adding anything else)
0.69g –Leu DO Supplement
18g Agar
Make up to 1l with water and autoclave.
Pour plates in a lamina flow cabinet and allow to dry for 1 hour before storing.
Store at room temperature in the dark.
SD-trp Liquid media (1l)
Add ~500ml MilliQ water to a 1l bottle
26.7g Minimal SD base (dissolve with a stirrer before adding anything else)
0.74g –Trp DO Supplement
Make up to 1l with water and autoclave.
Store at room temperature.
SD-trp Plates (1l)
Add ~500ml MilliQ water to a 1l bottle
26.7g Minimal SD base (dissolve with a stirrer before adding anything else)
0.74g –Trp DO Supplement
18g Agar
Make up to 1l with water and autoclave.
Pour plates in a lamina flow cabinet and allow to dry for 1 hour before storing.
Store at room temperature in the dark.
SD-leu-trp Liquid media (1l)
Add ~500ml MilliQ water to a 1l bottle
26.7g Minimal SD base (dissolve with a stirrer before adding anything else)
0.64g –Leu/–Trp DO Supplement
Make up to 1l with water and autoclave.
Store at room temperature.
SD-leu-trp Plates (1l)
Add ~500ml MilliQ water to a 1l bottle
26.7g Minimal SD base (dissolve with a stirrer before adding anything else)
0.64g –Leu/–Trp DO Supplement
18g Agar
Make up to 1l with water and autoclave.
Pour plates in a lamina flow cabinet and allow to dry for 1 hour before storing.
Store at room temperature in the dark.
SD-leu- his Liquid media (1l)
Add ~500ml MilliQ water to a 1l bottle
26.7g Minimal SD base (dissolve with a stirrer before adding anything else)
0.62g –His/–Leu/–Trp DO Supplement
0.2g Tryptophan
Make up to 1l with water and autoclave.
Store at room temperature.
SD-leu-his Plates (1l)
Add ~500ml MilliQ water to a 1l bottle
26.7g Minimal SD base (dissolve with a stirrer before adding anything else)
0.62g –His/–Leu/–Trp DO Supplement
0.2g Tryptophan
18g Agar
Make up to 1l with water and autoclave.
Pour plates in a lamina flow cabinet and allow to dry for 1 hour before storing.
Store at room temperature in the dark.
SD-leu- his + 3AT Plates (1l)
Add ~500ml MilliQ water to a 1l bottle
26.7g Minimal SD base (dissolve with a stirrer before adding anything else)
0.62g –His/–Leu/–Trp DO Supplement
0.2g Tryptophan
18g Agar
Make up to 1l with water and autoclave.
Allow media to cool to ~50oC.
Weigh out appropriate amount of 3AT in extraction hood and add to media. It should dissolve immediately.
Pour plates in a lamina flow cabinet and allow to dry for 1 hour before storing.
Store at room temperature in the dark for short term storage or at 4oC for long term storage.
SD-leu-trp-his Liquid media (1l)
Add ~500ml MilliQ water to a 1l bottle
26.7g Minimal SD base (dissolve with a stirrer before adding anything else)
0.62g –His/–Leu/–Trp DO Supplement
Make up to 1l with water and autoclave.
Store at room temperature.
SD-leu-trp-his Plates (1l)
Add ~500ml MilliQ water to a 1l bottle
26.7g Minimal SD base (dissolve with a stirrer before adding anything else)
0.62g –His/–Leu/–Trp DO Supplement
18g Agar
Make up to 1l with water and autoclave.
Pour plates in a lamina flow cabinet and allow to dry for 1 hour before storing.
Store at room temperature in the dark.
SD-leu-trp-his + 3AT Plates (1l)
Add ~500ml MilliQ water to a 1l bottle
26.7g Minimal SD base (dissolve with a stirrer before adding anything else)
0.62g –His/–Leu/–Trp DO Supplement
18g Agar
Make up to 1l with water and autoclave.
Allow media to cool to ~50oC.
Weigh out appropriate amount of 3AT in extraction hood and add to media. It should dissolve immediately.
Pour plates in a lamina flow cabinet and allow to dry for 1 hour before storing.
Store at room temperature in the dark for short term storage or at 4oC for long term storage.