Xiuzhu Xu A, Shuixia Chen A, B * , Linzhou Zhuang A, Chunhao Zhenga, Yingzhu Wu A

Establishment of a Novel Surface Imprinting System for Melamine as an N-containing Template on Polypropylene Fibers and Mechanism of Template-matrix Interactions

Xiuzhu Xu a, Shuixia Chen a, b[*], Linzhou Zhuang a, Chunhao Zhenga, Yingzhu Wu a

a. PCFM Lab, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, PR China

b. DSAPM Lab, Materials Science Institute, Sun Yat-Sen University, Guangzhou 510275, PR China

Supporting information

I. Determination of Mel and diNA concentration in mixed aqueous solution with two-band method

We developed a simultaneous equation method based on Beer’s Law to determine the concentrations of Mel and diNA in mixture solution respectively. Standard stock solutions of Mel and diNA were scanned by a UV spectrophotometer from 200 to 600 nm to obtain the absorbance spectra. Their UV-vis spectra were provided in Figure S1. In our method, two wavelengths 230 and 292 nm were selected for the development of the simultaneous equations. Both Mel and diNA obeyed Beer-Lambert law in the concentration range of 5 to 100 mg/L at 230 and 292 nm. Therefore, standard stock solutions of Mel and diNA were measured at 230 and 292 nm respectively to obtain calibration curves.

The linear regression equation plotted for Mel was:

(1)

And the calibration curves for diNA were:

(2)

(3)

where A1 was the adsorbances of Mel measured at 230 nm, respectively. A2 , A3 were the adsorbance of diNA at 230 and 292 nm. CMel and CdiNA were concentrations of Mel and diNA respectively.

Thus the simultaneous equations were developed according to Beer’s Law as follows:

(4)

where A292, A230 were the adsorbances of mixture solution of Mel and diNA measured at 292 and 230 nm, respectively.

As a result, the concentrations of Mel and diNA in mixture solution could be determined by measuring the adsorbances of mixture solution at 230 and 292 nm and then resolving the simultaneous equations described above.

Figure S1. UV-vis spectra of Mel and diNA

[*] Corresponding author. Email: ; Fax +86-20-84034027