WFD Intercalibration Phase 2 : Milestone 2 Report (For ECOSTAT Meeting 8-9 April 2009)

WFD Intercalibration Phase 2 : Milestone 2 Report (For ECOSTAT Meeting 8-9 April 2009)

/ EUROPEAN COMMISSION
DIRECTORATE GENERAL JRC
JOINT RESEARCH CENTRE
Institute of Environment and Sustainability

WFD Intercalibration Phase 2 : Milestone 2 report (for ECOSTAT meeting 8-9 April 2009)

The new IC guidance foresees a ‘Milestone 2’ report for the spring of 2010, with the following key elements:

- Overview of the national assessment methods that will be intercalibrated;

- Results of WFD compliance and feasibility check;

- Data set collection;

- Design of IC procedure and development of IC common metric;

- Progress on Benchmarking and Boundary comparison/setting.

All GIGs are kindly requested to submit their progress reports for the relevant quality elements following this template as much as possible.

Also, you are requested to update the relevant sections of the intercalibration work plan (distributed as a separate document).

Depending on how the work has been organized, we expect one response for each quality element for each of the GIGs. In case of horizontal activities (e.g. large rivers) or where the work is carried out cross-GIG (e.g. fish in rivers), one coordinated response is expected.

The questions in the reporting template follow the new intercalibration guidance as closely as possible. Please contact the IC steering group if you need any further clarifications:

 Wouter van de Bund - Rivers;

 Sandra Poikane - Lakes;

 Wendy Bonne - Coastal waters;

 Nikolaos Zampoukas - Transitional waters.

Please send your responses before 22nd March 2010 both to and for coastal and transitional waters and also where Mediterranean lagoons are involved.

Water category/GIG/BQE/ horizontal activity: / GIG Black Sea/Macroinvertebrates
Information provided by: / RO: Gabriel Chiriac, Romanian Water Directorate and Ramona Bercea Dobrogea Litoral Water Directorate BG: Mrs. Elitsa Hineva, Black Sea Basin Directorate, Varna, Bulgaria

1: Organisation

1.1. Responsibilities

Please indicate how the work is organised, indicating the lead country/person and the list of involved experts of every country (Provide your list of experts to update the attached contact overview where needed / when not done yet)

The new GIG Black Sea Coordinator: Gabriel Chiriac, Romania

Romania lead: Otilia Antonaru

Bulgaria lead: Elitsa Hineva

BQE-Romanian proposals:

Macroinvertebrates: Camelia Dumitrache (lead), Valeria Abaza, Ramona Bercea, Virginia Schroder, Luminita Gheorghe

Bulgaria: Black sea Basin Directorate (BSBD) is a water management authority created with the aim for implementation of integrated water management principles according to the requirements of WFD 2000/60. It is a regional structure to the Ministry of Environment and waters. The geographical area managed by BSBD is covering the Bulgarian part of Black sea watershed area, Black sea coastal waters and ground waters.

Responsible phytoplankton expert: Mrs. Violeta Velikova

Responsible macrozoobenthos expert: Mrs. Valentina Todorova

Responsible macrphytobenthos expert: Mr. Dimitar Berov

Contact person: Elitsa Hineva

The following overview clarifies which countries have checked their contacts in the list:

X = checked / Baltic Sea GIG / NEA GIG
Checked in general by Janet Cowden / Mediterranean Sea GIG / Black Sea GIG
Country / CW / TW
Estonia
Latvia
Lithuania
Poland / X
Finland
Sweden
Germany
Denmark
Norway / X
The Netherlands / X
Belgium / X
Ireland
UK / X
Portugal
Spain / X / X / X
France / X
Italy / X (some emails lacking)
Greece / X
Slovenia
Malta
Cyprus / X
Bulgaria / X
Romania / X
(emails lacking)

1.2. Participation

Please indicate which countries are participating in your group. Are there any difficulties with the participation of specific Member States? If yes, please specify

The participating countries: Romania and Bulgaria

1.3. Meetings

Please list the meetings of the group (including planned future meetings) in 2010

13.01.2010, Varna, Bulgaria

Planned technical meetings:

- Spring 2010 - April (in Constanta?), Autumn 2010 - Octombrie/November (in Varna?)

Common sampling campaign(s) – not established yet

2. Overview of Methods to be intercalibrated

Identify for each MS:

- the national classification method that will be intercalibrated

- the status of the method (e.g., finalized formally agreed national method, intercalibratable finalized method (scientifically ready for intercalibration, maybe not formally approved by authorities yet), method under development, no method yet)

- whether the WISER questionnare has been completed for the method

Country / Method / Status / WISER
questionnaire
RO / Assessment method for coastal waters using macrozoobenthos / 1 - finalized formally agreed national method
2 - intercalibratable finalized method
3 - method under development
4 - no method yet / 1- Completed
2- Completed partially
3- Not submitted
BG / Shannon index / 2 - intercalibratable finalized method / 1- Completed
BG / AMBI / 2 - intercalibratable finalized method / 1- Completed
BG / M - AMBI / 2 - intercalibratable finalized method / 1- Completed

Do you have sufficient information of the national classification methods that will be intercalibrated to carry out the compliance and feasibility checks? Please, specify information gaps

Country / Information gaps (including gaps in the WISER questionnaire)
Romania / WFD compliance ass. method, common IC type, inferential and historical data for RC, theoretical pressure-impact relationship, ecological status boundaries, EQR, appropriate IC option
Bulgaria

3. Checking of compliance of national assessment methods with the WFD requirements

What is the progress with the compliance checking? (1 – completed; 2 – in progress; 3 – not started); in case of ‘completed’ or ‘in progress’, please fill in the detailed information below; if ‘not started’, indicate the reasons.

Make separate considerations for coastal and transitional waters.

Romania
Coastal waters: completed
Transitional waters: in progress
CW - Pressure-impact relationships have to be tested.
TW – are not in included in the IC process at GIG Black Sea level
Bulgaria:
Coastal waters: completed
Transitional waters: in progress
CW - Pressure-impact relationships have to be tested.
TW – are not in included in the IC process at GIG Black Sea level

Describe the WFD compliance checking process and results (the table below lists the criteria from the IC guidance with relevant WISER questionnaire questions, please add more criteria where considered relevant).

Make a separate table for coastal and transitional waters.

This refers to Question 1 in the IC guidance: Q1. Do all national assessment methods meet the requirements of the Water Framework Directive?

Compliance criteria / Compliance checking conclusions
  1. Ecological status is classified by one of five classes (high, good, moderate, poor and bad).
/ Specify for
Romania, Bulgaria: yes
  1. High, good and moderate ecological status are set in line with the WFD’s normative definitions (Boundary setting procedure)
See info from WISER Questionnaires: / BG: not yet
- Question A.12: Scope of detected pressures / RO: Specify for
eutrophication and general degradation and
aquatic habitat destruction
BG: Not all types of possible pressures registered in Black sea are encompassed by the status assessment indices.
- Question A.13: Has the pressure-impact relationship of the assessment method been tested? / RO: Not yet
BG: Not yet. It is planned to be done during the second IC exercise.
- Question C.14: Setting of ecological status boundaries: methodology and reasoning to derive and set boundaries / RO:-Equidistant division of the EQR gradient (e.g. boundary setting at 0.8, 0.6, 0.4, 0.2)
-Calibrated against pre-classified sampling sites (e.g. pre-classification based on expert judgement)
BG: 1.Shannon index: Identified 6 WB types are grouped into 2 groups depending on the most important for the distribution of macrozoobenthos factor: bottom substrate. Classification system is developed for types with muddy and sandy/mixed substrate. Because of lack of historical data reference values are developed from the current best available conditions. Based on expert judgment it is supposed that in WB with mixed/sand substrate in current best condition the average Shannon index constitutes 75 % of the expected average reference conditions and for WB with muddy substrate – respectively 70 %. An additional criterion is used for WB with muddy substrate more that 25 species per sample (0.1 m2) for the determination of the best current conditions on base of which reference conditions should be developed. The boundaries between the different water status classes are determined as it is assumed that each class range (20%) is covering equal ranges from the whole status range (100%).
2. AMBI – the reference value is derived on the basis of the domination of sensitive and indifferent species and the value for bad condition is derived on the basis of domination of opportunists from first and second order. Boundary values are the same as derived by Borja et al., (2000, 2003) and Muxica et al., (2005). The estimation of the index is optimized by reclassification from one ecological group to another because they have shown high ecological similarity with the species from the new group under Black Sea conditions.
3. M-AMBI – the boundaries between classes are the same as determined as a result of the intercalibration process for the North Atlantic ocean (Borja et al., 2006). The index is adopted for the conditions of the Black sea by setting specific boundaries for high/good and poor/bad status of the Shannon index and number of species S.
(The referent conditions and classification system is developed by Mrs. Valentina Todorova, Institute of Oceanology, Varna).
- Question C.15: Boundary setting procedure in relation to the pressure:
Which amount of data/pressure indicators have been related to the method and what was the outcome of the relation? / RO: Macroinvertebrates were placed into five groups in relation with their sensitivity to an increasing stress gradient. These five groups are as follows:
(I). Species very sensitive to organic enrichment and present under unpolluted conditions (initial state)
(II). Species indifferent to enrichment
(III). Species tolerant to excess organic matter enrichment
(IV).Second-order opportunistic species
(V). First-order opportunistic species
Boundaries for HG and GM were determined from this relationship:
The HG boundary was identified as the point at which all tolerant species were on average <10% of cover.
- The GM boundary was the point at which the lower confidence limits of the sensitive and upper confidence limit of the tolerant species intersect. At this point there is still a high probability of having >50% cover of sensitive species and no more than 50% cover of tolerant species. This would be indicative of slight change, the community could still easily recover to its original status. The highly sensitive species are still present (10-50% cover) and highly tolerant (undesirable) species would be <20% cover.
- The MP boundary was set where the lower confidence limit of the sensitive and upper confidence limit of the tolerant species intersect. At this point there is a low probability that sensitive species would be at 50% cover, but a high probability that tolerant species would be at 50% cover. Very sensitive species are still present, but the community has thus undergone a moderate change.
- The PB boundary is a point at which highly sensitive species are extinct and there are very few sensitive species. Here the community is dominated by tolerant species.
BG: Not done yet. It is planned to be done during the second IC exercise.
- Question C.11 + C.16: Reference and Good status community description:
Is a description of the communities of reference/high – good – moderate status provided? Not only a formula or an EQR value, but the range of values for the different parameters included in the method that result in high – good – moderate status / RO: Soft bottom communities with high number of sensitive taxa, and high abundance, the most characteristic species being different according to grain size: Donacilla cornea, Donax trunculus, Lentidium mediterraneum, Spio filicornis and others.
At good status stands of the sensitive taxa are well developed, but significantly decreasing at good-moderate boundary and replaced by tolerant taxa.
BG: Not done yet.
Compliance criteria / Compliance checking conclusions
  1. All relevant parameters indicative of the biological quality element are covered (see Table 1 in the IC Guidance). A combination rule to combine parameter assessment into BQE assessment has to be defined. If parameters are missing, Member States need to demonstrate that the method is sufficiently indicative of the status of the QE as a whole
See info from WISER Questionnaires: / BG: Yes
- Question C.01: Complete list of biological metric(s) used in assessment / RO: List of species, abundance of taxa and biomass (wet weight) of taxa;
BG: Shannon diversity index, AMBI, M-AMBI index.
- Question C.02: Data basis for metric calculation / RO: Data from single sampling/survey occasion in time ;Data from single spatial replicate
BG: list of groups at the lowest possible taxonomic level of identification, abundance and biomass by each group presented in the sample. Three samples are taken at each point to reduce the uncertainty. list of groups at the lowest possible taxonomic level of identification, abundance and biomass by each group presented in the sample. Three samples are taken at each point to reduce the uncertainty.
- Question C.04: Combination rule for multimetrics / RO: average metric scores
BG: The general combination rule is “one out – all out” applied for each index. In some cases based on expert judgment greater emphasis is put on M-AMBI as it combines the other two indices in itself. The general combination rule is “one out – all out” applied for each index. In some cases based on expert judgment greater emphasis is put on M-AMBI as it combines the other two indices in itself.
  1. Assessment is adapted to intercalibration common types that are defined in line with the typological requirements of the Annex II WFD and approved by WG ECOSTAT
See info from WISER Questionnaires:
- Question A.14: Is the assessment method applied to water bodies in the whole country? / RO: Organisms of the complete sample are identified
BG: Yes
- Question A.15: Specify common intercalibration types / CW-BL1
- Question C.03: Does the selection of metrics differ between types of water bodies? / RO: no
BG: No. (There’re no WB with only rocky substrate where metrics are inapplicable)
  1. The water body is assessed against type-specific near-natural reference conditions
See info from WISER Questionnaires:
- Question C.05: Scope of reference conditions / RO: Habitat-specific
- Question C.06: Key source(s) to derive reference conditions / RO: expert knowledge, historical data
BG: Best available current conditions (low pressure) along the Black Sea coast.
- Question C.07-C.08-C.09: Number of sites, location and geographical coverage of sites used to derive reference conditions / RO: 11 sites at the Romanian Black Sea
All Romanian Black Sea shore between Sulina (in North) and Vama Veche (in South)
Sulina, Mila 9, Sf. Gheorghe, Portita, Chituc, Gura Buhaz, Constanta, Eforie South, Costinesti, Mangalia, Vama Veche
BG: 19 points. Current conditions (low pressure) available in front of the Black Sea coast.
- Question C.10: Time period (months+years) of data of sites used to derive reference conditions / RO: Historical data between 1960s and 1980s
BG: 2002-2006 (March, June, July, August, September, November – different month in different years)
- Question C.12: Reference site characterisation: criteria to select them / RO: The soft bottom communities suffered in the last four decades different modifications due to ecological changes occurrence. In the last decade, nutrient inputs from the inland waters (Danube River) significantly decreased, allowing to sensitive species (mentioned above at C-11) to develop stable and abundant populations.
BG: Low pressure
- Is a true reference used for the definition of High status or an alternative benchmark estimation? / RO and BG : an alternative benchmark estimation
  1. Assessment results are expressed as EQRs:
- Question C.13: Are the assessment results expressed as Ecological Quality Ratios (EQR)? / RO and BG: yes
  1. Sampling procedure allows for representative information about water body quality/ecological status in space and time
See info from WISER Questionnaires:
- Question B.08: How many sampling/survey occasions (in time) are required to allow for ecological quality classifica-tion of sampling/survey site or area? / RO: One occasion per sampling season
BG: One sampling per year is assumed to be enough.
- Question B.09: Sampling/survey months / RO: February, April to July, October-November
BG: Summer season
- Question B.10: Which method is used to select the sampling /survey site or area? / RO: Expert knowledge (e.g. sites most representative of water body)
BG: The points are chosen so that to reflect the representative for the WB type specific conditions (e.g. bottom substrate on the sampling location is the same as the determined for the WB type, small spatial variations are avoided) as well as “averaged” impact from the land based sources (i.e. not to close to the coast where they could react to local influences).
- Question B.11: How many spatial replicates per sampling/survey occasion are required to allow for ecological quality classification of sampling/survey site or area? / RO: 24 replicates (one per sampling site)
BG: 3 replicates
- Question B.12: Total sampled area or volume, or total surveyed area, or total sampling duration on which ecological quality classification of sampling/survey site or area is based / RO: Sum of 24 spatial replicates a 0.05 square-metres = 1.2 square-metres of soft-bottom in total
BG: 3x0.10 m2.
- Question C.17: Has the uncertainty of the method been quantified and is it regarded in the assessment ? / no (to be done)
- Question C.18: Specify how the uncertainty has been quantified and regarded
Compliance criteria / Compliance checking conclusions
  1. All data relevant for assessing the biological parameters specified in the WFD’s normative definitions are covered by the sampling procedure
See info from WISER Questionnaires:
- Question B.13-B.14-B.15: Short description of field sampling/survey procedure and processing (sub-sampling) / RO: Single-sampling is carried out. A sample consists of 24 "sampling units" taken from all soft-bottom habitat types. A "sampling unit" is a stationary sampling performed with Van Veen Grab long arm from an area of 0.25 x 0.2 m.
Organisms of the complete sample are identified.
BG: “For sampling is used sampler type Van veen (are 0.1m2). At each point are gathered 3 samples. On board a ship samples are washed through sieves (aperture mesh size 1.0x1.0mm; 0.5x0.5mm) and then fixed in formaldehyde/coastal water solution 4-10%. Laboratory analysis includes sorting, determination of taxonomic composition up to species or the lowest possible over species level (Marinov 1997, Fauvel, 1923, 1927, Morduhay-Boltovskoy, 1968, 1969, 1972) and determination of abundance and biomass.” (Todorova V, Ts. Konsulova, 2009 Report “Biological monitoring of coastal water in Black sea basin area benthic invertebrate fauna 2008”)
  1. Selected taxonomic level achieves adequate confidence and precision in classification
See info from WISER Questionnaires: / BG: yes
- Question B.02-B.03: Sampling/survey device / RO: Grab. Van Veen Grab long arm
BG: sampler type Van veen (are 0.1m2)
- Question B.04: Minimum size of organisms sampled and processed / RO: 0.250 mm
- Question B.16-B.17: Record of biological data: level of taxonomical identification – what groups to which level / RO: Species/species groups level
Genus level
Other level
Molluscs, polychaetes, amphipods, isopods, decapods, tanaides, cumaceans are identified to species/genus level. Others, like oligochaetes, chironomids, Harpacticoids, nematodes to level of order. BG: Species level, lowest possible over species level
  1. Other criteria 1

  1. Other criteria 2

  1. Other criteria 3

Summarise the conclusions of the compliance checking