WEB TABLE E. Sequence family variants
Kuroda-Kawaguchi, et al. The AZFc region of the Y chromosome features massive palindromes and uniform recurrent deletions in infertile men. Nature Genetics 2001. Supplementary information.
Below are listed the 35 SFVs for which AZFc region BACs were typed. All SFV typing information on all 220 BACs examined was consistent with the map shown in Fig. 1. For example, the green amplicons g1, g2, and g3 differ at three clustered SFVs (SFVs 1, 6, and 7 as listed below), with bases G/T/T in g1, A/C/G in g2, and G/T/G in g3. All tested BACs that contain these SFV sites proved to be of the g1, the g2, or the g3 type, with no BAC exhibiting an alternative pattern.
1.
Forward primer:TCAGTCCACCTATGTGCTGG
Reverse primer:ATTCCTTTCCTTGGGTGGAT
Amplicons (see Fig. 1a):[green1,green3/green2]
Sequence:
TCAGTCCACCTATGTGCTGGACTCCGCAATATGTAACAACCCTCTCTCTTGGTAGAGTCTAGAATATGAAGGAGAGTCACATCATGTAGGTTTTTCAATCAGCGGTATGTCACAATTTGTTTGCTGAGCAAGGCTCAGGCAGGAGGTGAGA[G/A]TCACATTACCTAGATGTTAAGCCAAACAATATTTCACAATGTCTTCTGGGTGCAGGACACTGTCAGAAGAGACAAATCCTCTAGCTTATAGACCCATAGATATGTGATAATATCCCCTTTTGGCAGGGTCCAGACAGAAGAGACACATTATGATTCTAACACAGTGATATGTTACAATCCACCCAAGGAAAGGAAT
2.
Forward primer:TCACCAGTGAATGTGCAAAAA
Reverse primer:GGGATTACAGGCATACACAAA
Amplicons (see Fig. 1a):[green1,green2/green3]
Sequence:
TCACCAGTGAATGTGCAAAAAAAGCAACAATGGCACATGCCTTTTTCTCTGGGAGCTCCATCCCAGGGAGGTATAGACCTGTTTTCAGCCCAAAAGCACTTGTAGGAAGTAGCCAGAAATGCCTGTGGAAAGGTCTTCCCCAGTGAAAAGAAAATGACTGGGGATCCACTTAAGAAAGCAGCCTACTGGGTGCAGCAGCTCTTGCCTGTAGTCTCAGCACTTTGGGAGGCTGAGGTGGG[C/T]GGATCAACCTGAGGTCCGGAGTTGGAGACCAGCCTGAACCAACATGGTGAAACCCCATCTCTACTAAAAATACAAAATTAGCCAGGTGTGTTTGTGTATGCCTGTAATCCC
3.
Forward primer: GTCAGAAAATTTTGCGCAGG
Reverse primer: TACAGGCATAAGCCACCACA
Amplicons (see Fig. 1a):[green1,green2/green3]
Sequence:
GTCAGAAAATTTTGCGCAGGCATCCATCTTGTTTTATGTTTTCAGGAACATGACCTGAACCAATGTGGCTCTGTTTTGTTTTGGCCTCTGCCATTTTACAATGGTGGCCCAGGTTTAATCCTGGCTTAAGGAAAGAGTCTCTTCTGGTTTTATATCAGCATGTAACTTTTGCCCTTTGCTGATTCTCTTCCACTTAACAAACTGCCTTACTTTTTTTTTTTTTTT[/T]CCTCTGAGCACCTGGTAAGTTACTTTTGTTAAAATTTGAAAGCCAGAAACACTGGCTGCTTGGTGCTACTAGAGTCAGGTAATAAAAAAATTAAAAGGATATTCTTAAAGAGTCCTCAACTTAATTAAAAGTGGATATCCAAATTATAGGTATATTTAAAAGACCTTTATATTTTTCTTTTCCTGGATCTTGTTTTGCTGGTAGAAGTTTTTTTCTCAGTCAACTGAATCCTTTTTCTTCATTTTGTCTGGCCACTCTAATGCATGCATGAGAGGACAGTAATTCAATCTGGAAATTGGCAAATAAAAAACCTTATGACAGCTGGGTGTGGTGGCTTATGCCTGTA
4.
Forward primer:TGTTGGCAATCATAACTGGG
Reverse primer:GGGTGGAAGCATAGGAGTCA
Amplicons (see Fig. 1a):[green1/green2,green3]
Sequence:
TGTTGGCAATCATAACTGGGTTTAAGGCAATATGTAAGATTGTGAGTGAATATAAGCATGTAGGCCTCAGAGTGGTTTGTAACTCTCATGCATGTTGCATAAAGCCTCTGGATGTTGTAGAGTGTGTCATATAATGATGTAGAACACGTGTAAGGTTGTGACTGTAATATACATATCAAGCTAAAAGTTAAAGGTGTCACCTAAAAGATGAGGAGATTGTGTCATATTACCAGACCTAGTACCCTGGTGTTGAGATTTCTGTCTTAAATTCTTTACCACGGGTGCGTTGTGAAATATTACTCTGTGTGTATGTGTTTATGTGTGTTTGTGTATATATATATAATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT[/GT]ATTATGCTCTCATAGTGCCAGGTAGACAGCCCTACTGTTGAACATGGACAAGTCACCAGGACATTCCAAAATAATAGTATGGGTGGTTTTTTTGATGTTTGAAGAATGACTCAGCAGACATGTAGTCCTTTGACTCTCCTACTGGAATACAATCTTCAAGTGGGAATGGGGCTTTTATACATGGACCTTGCCCACTGATGACATTGTGACTCCTATGCTTCCACCC
5.
Forward primer:TGATGTTTGCCACCTTCTTTC
Reverse primer:TGCTGCAGACAATGTGGA
Amplicons (see Fig. 1a):[yellow1/yellow2]
Sequence:
TGATGTTTGCCACCTTCTTTCATGTCCCTGCAAAAGACATGAACTCATCCACTTTTACGGCTGCACACTATCCCATGGTGCATATGTGTCACCTTTTCTTTATTCAGTCTATTATCAATGCACATTTGGGTTGTTTCCAAGTGTTTGCTACTGTGAATAGTG[C/A]TGCGATAAATGTACATGTGCATGTGTCTTTATAGTAGAATGATTTATAGTCCTTTGGTTATATGCGCAGTAAGGGGATTGCTGGGTCAAATGGTATTTCTCGTTCTAGATCCTAGTCTTCCACAAGGGTTAAATTATTAACAAACCCACCAACACTGTAAAAGCATTTCTATTTCTCCACATTGTCTGCAGCA
6.
Forward primer:GCCATCCTCCTGAGTGAATC
Reverse primer:GCCAAGCTCTATTAAGCCCA
Amplicons (see Fig. 1a):[green1,green3/green2]
Sequence:
GCCATCCTCCTGAGTGAATCTCAAAATCCTCCTCTCTGTTCTTTTCTGCATCAGGTTTTTGATTGCAGAGCACAGATATGTGCAGTCATAGGTTTGTCAGG[T/C]GAGGTGGTCACTGATCTTATTCCAGCTTTGCATCATATAAATAGCAAATAACACAAAACAATCATGAGTATAATTAACAACACTTCTTCCAGTCAGAGTGACTTGCAGAGTTACTTGCCATCTGAGTTCACTCTGTGCTATTAATGAGGAACCTCACTGGGGGAATGTTGATTTCCTTTTATTTAAGCAGTGATGTTGTTAGAAGCTGGAAAGGGGGTGTTAGTCTAAGTACTAGGGTAGAACAAAGGAGGTTACAAGATGGGCTTAATAGAGCTTGGC
7.
Forward primer:GGACAAGACATTGGCAGGAG
Reverse primer:GCAATTCTCACGCAGGTTTC
Amplicons (see Fig. 1a):[green1/green2,green3]
Sequence:
GGACAAGACATTGGCAGGAGAGACATATTCCTTAGCTGAAAGACACAGAGATGTTTGATAACATCCACTGTTTGCAGGGCTCAGGAGAAGAATTACATTATGATGATTCTGTCCCAGTGATATGTAACAAGGCATTCA[T/G]GAAAAGGAATTTGACGCAAAAAGTATCAAAACCTGGGCACTAGGCCTAGTGATACGACACAATATCTTCATCTTTGAGGGTAACACCTGTAAATTTTGGCTGACCATGTATATTAGTGTCACAATCTCATGTGTATGCTGGGCCAATATATTACACTCTGTAAAATACCCGTGGGTGTTATGAAACCTGCGTGAGAATTGC
8.
Forward primer:AAGCTCTTTGCTTCAGATTTTCA
Reverse primer:TGCTGTACACATGGTTTGGG
Amplicons (see Fig. 1a):[gray1/gray2]
Sequence:
AAGCTCTTTGCTTCAGATTTTCATTTAGATTCTTACATATATTTTATAAATGCACATGCATGTTAACCAAGTTTTTTTTGTGATAATGACCATGTATTGAGCATGACCACATGCTAGGCACTGTGATGGATGATTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT[/GTGT]ATCCTAAACCATATAACAACTCTGTAAATAAGCATCCTCATCACTATTTTGGGTGATGAACCAAATAAGATTCAATGAGGCTGAAGAACTTGATCATATCAGTCAGTTAAGCAGGGGTTAGAATTTCAACCCAGGATATCTGACCTCAAATACACAACCTTCCCAAACCATGTGTACAGCA
9.
Forward primer:AGGTGGGAGCATCCCTTAAT
Reverse primer:TCTGAGCAATTCATTTCAAGATT
Amplicons (see Fig. 1a):[blue1/blue2/blue3,blue4]
Sequence:
AGGTGGGAGCATCCCTTAATATTGGGAGTTTGAGGCTAATGTGAGTCACACCACTGCATTTTAGCCTGGACAATAGAGGGAGAGTCTCTCTCTCTCTCACTCTCT[/CTCTCTCT/CTCTCTCTCT]CTCTCTCTCTCTCTCTCTCTGTCTCTGTCTCTATCTCTCTCTCTTTCCCTATCTCTGTCTCACACACACAAACAAAAATAAATAAATAAATAGAAAAGAAACATATTCAGTAACAGTCTCAATGTTTTACTTGCTTTATAATGATATCATAATCTTGAAATGAATTGCTCAGA
10.
Forward primer:GCCTGTAAACAGGTCTCCAA
Reverse primer:AGTGTGGTGCTGGGAAAAAG
Amplicons (see Fig. 1a):[red2/red1,red3,red4]
GCCTGTAAACAGGTCTCCAACTCATTTTGTTAAAAGTTTTCTTCTTCTTCTTCTTTTTTTTTTTTCCATTTTCCCCAATCAACTCTACTCCTCATCCTTCAGAGCGTCCCTGACTTAATATATTCTAGTCATGTGACCAGAACCCAATTTTTTTTGATGTAAGGAAAG[C/T]GTTCTGCAACATGAGGATTTGCAACCGTCGTTTAAGATAAGCTGAGTATACGCATGTGGGTTACTTTCTGGCTTCTCTTATCTCTTTCAACATTTATTTTTCTGTCTTTTTCCCAGCACCACACT
11.
Forward primer:CAAAGCAAACTTAAAATCCACG
Reverse primer:AAGAGAATTCACACTGCAGGAA
Amplicons (see Fig. 1a):[green1,green2/green3]
Sequence:
CAAAGCAAACTTAAAATCCACGTAGTTTTTTTTTAAGAGAAAGAAGGCATTCCATAATAGTAAAATGCAATAGACAAACCCAACCCAAAAGCACTGAGTATAAAGCGAGTTCTTAG[T/A]GATGCTGCAAGATGTTAAGTTAAATCTTCTTCAACAGTCATTACCTTTATGTGGCATTTGTTCAGTATTAAGTCTCTGATGTTGGACATAAAAT[G/C]AGGATGCTTTAAAAGCTTTTCCACATTTTTAACACTTGTGAAGCTTCTCTCTACTATGAGTTCTATTATTTCTAATGAAGTGTGAGAATGAACTTAATGTTTCACCACATTCTTCACATTTGTAGGCTTTTCCTGCAGTGTGAATTCTCTT
12.
Forward primer:CAAGATGCTGAGGCAGGAC
Reverse primer:AAGTGTTTGGTCTTGGGAGG
Amplicons (see Fig. 1a):[blue2,blue3,blue4/blue1]
Sequence:
CAAGATGCTGAGGCAGGACAATAGATTGGACCCAGAAGGTGGAGGTTGTAGTAAGCCAAGATGGAGCCACTGCACCCCTGCTTGGGTGACAAAGCTACATCCCATCTCAAAACAAACAAACAAACAAACAAA[CAAA/]AAACTGATAAAATTCCAACCACTCTAGATTATTCCTATTTGTAACAACTTATTACTACACCATGACTTACAACAACCATTGTCAAAACTTTTAAGAAAAAAATAACATGACTACCTCCCAAGACCAAACACTT
13.
Forward primer:CCCTTTTATGAAAAGTTGTGGTG
Reverse primer:ACTCCTTCCAGGAGGTGCTT
Amplicons (see Fig. 1a):[blue1,blue2/blue3/blue4]
Sequence:
CCCTTTTATGAAAAGTTGTGGTGAGTAATGAAGAATAACAAGTAATACCACAACACCAATAATGTTCATTTTTGACAATGAAAATAGATTTTATGGTTGTATGTCCACAGTCAACATTCACAAATTCACATACATATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT[/GTAT/GTGTAT]ATATAAATACAAAAATGTACATATATGTATTAGGTACTGTGTGACATCAGTGCTGGGTGTCCAGGAGACACAGACAGACAGACAAGAAAACTAATGTGACAGATAAGAAGCACCTCCTGGAAGGAGT
14.
Forward primer:GGCTATGGTGAGCCATGATT
Reverse primer:GGACTCCCTTTGGAGACTGTT
Amplicons (see Fig. 1a):[green1/green2,green3]
Sequence:
GGCTATGGTGAGCCATGATTATAGCATTGCACTCCAGCCTGGGTGACACAGCTAAATATTGACTCAAACATTTTAGAATATTTTACTACTTCTGCAAAACAAAAACAAAAACAACAACAACAA[C/]AAAAAACTATTGGCTCTTTGAAAGAGTTCTCATTGACCTGTAGATCATTTCTAATACCATTAACTGAGGCACCACTCTTTTCATGAGGGCATTAGCTGCAGGGAGTCTGTCCCTTGCAGACCCCTGACCGAGCGACAGATGAATAAAGTACCCTGTCACACAGATATTCTGCTTTTCAAGTCCAGCTGAGGGTCTGGGGCCACTAACAGTCTCCAAAGGGAGTCC
15.
Forward primer:CCTCTGAGGACAGCCTACTTG
Reverse primer:TCTAAAACAGGTAACAGTTGGAACA
Amplicons (see Fig. 1a):[green1/green2,green3]
Sequence:
CCTCTGAGGACAGCCTACTTGTTCTGCAACTTGCAACCACTGGATTCTAAGGGGTGAGCATCCAATTGTGTGTGCGTGAGTGTGTGTGT[/GT]ATGTGTGCGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTACATGGCCTTCTGCTTCATTTCCATGGGATGTTCTCCTAGAAGAAATTCCTAGGGAATCCTGGGACTGTGCTGGAGATTCTCAAACTGCCAAATTCAGTAAGAGTTGTCTCAAGAATATAATTAGTAGTATTTTCTGCAGATTACTATGTCAGGGTTTCCTTTGCCAAAAAAAAATGTGTAAAAAACAAAGTTGTTAGTCCTTTACATTATTTTAATTAGGTCATCAAATAAACCTTTTATTTTCTTCAAACTTATTTTTATGACAATCATTGAATTTTTGTTTTCTTCTTTGTTCCAACTGTTACCTGTTTTAGA
16.
Forward primer:GGAAGTTGCATTTCTGTCTGC
Reverse primer:CCTTTGCATCTTGGCACAG
Amplicons (see Fig. 1a):[blue1/blue2,blue3,blue4]
Sequence:
GGAAGTTGCATTTCTGTCTGCAGAAATTGTCCCTTTTTGTATTTTAATTTCATCAGAAGTATTGAGACGAGATGGGTGGTAATGAGAGAAAAAATTGTGTTGCTTCTCTCTTATTC[A/G]TGAAACTAACT[/AACT]CCCTGCTGATGACTTTAAAATAAGGAAGAGCAAAAGGGTGCAGAATCCTCCTGTAAGCCTATGAATTTTTCTTGTTATTAGCATTGCTAGCTTTATAAAAAAATAAGTTACGTGTCAGAACTTGAAGAGAAAGGTCAGTAAAATTGTTTCTGAGCATGAGATGTAATTAAACATGTGAGATGCAGAGATCTGTGCCAAGATGCAAAGG
17.
Forward primer:CAAGCCCTCAGAAATAATCTTA
Reverse primer:CTTTGTCAGATGAGTAGGTTGTG
Amplicons (see Fig. 1a):[yellow1/yellow2]
Sequence:
CAAGCCCTCAGAAATAATCTTACATATCTACAACTATCTGACAAACCTGAGAAAAACAAGCAATGGGGAAAGGATTCCCTATTTAATAAATGGTGCTGGGAAAACAGGCTAGCCATATGTAGAAAGCTGAAACTGGATCCCTTCCTTACACCTTATACAAAAATTAATTCAAGATGGATTAAA[G/A]ACTTAAATGTTAGACTTAAAACCATAAAAACCCTAGAAGAAAACCTAGGCAATACCATTCAGGACATAGGCATGGGCAAGGACTTCATGTCTAAAACACCAAAAGCATGCCAACAAAAGCCAAAATTGACAAATGGGATCTGTTTAAACTAAAGAGCTTCTGCACAGCAAAAGAAACTACTATCAGAGTGAAGAGGCAACCTACAGAATGGGAGAACATTTTCACAACCTACTCATCTGACAAAG
18.
Forward primer:GCTTGATCAGCTAGAACCAAA
Reverse primer:TGCCTAAGGGAGCTTAATTTTT
Amplicons (see Fig. 1a):[red1,red2/red4/red3]
Sequence:
GCTTGATCAGCTAGAACCAAATATTCTGGATAATCGTTAGTATGTACATAACCATAAATATATGCATATATAGACACATATGTACACATATATACACATAGGCACATAAAAGAATTACTAATTTTTTGCCTATTATTGGACACTAAACATCACAATTTAATCTTTGGTGATGCAGAAAGCACTGTTTGCTATCCTGCGTATCCTTGGAGTCAACTTTCTGGTTATGAATCAGCAAGCTTACTAACTAAT[GAAATACAGGA/GAAATAC/AGA]GTGTCTGAGGAGTTTCCTCTCCACTGGACTCAGCTTATAGAACTGAGTACTAACACTAAAGCTAGAAGAAGCGGTACTGATTTTTTTTTTT[T//]ATGGTATCCACAGGAAGTACCTGCAATATAAAAAAATTAAGCTCCCTTAGGCA
19.
Forward primer:CACCATACCTGGGTAACAGAGA
Reverse primer:CCGAAACTTATACCAAAAGAAGAAAA
Amplicons (see Fig. 1a):[red2/red3,red4/red1]
Sequence:
CACCATACCTGGGTAACAGAGAGACTCTTTCACGGAAGAAAAGAAAAAAGAGAAGAGAGAAGGAGGAAGGAAGGAGAGAAAGGAAGGAAAGAAGGAAGGAAGGAAGGAGGGAACAAAGGAAGGAAGGGAGGGATGGAGGGAGGAAGGAAGAGAAGGGAAGAAGGAAGGAAAGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGG[AAGGAAGGAAGG//AAGGAAGG]CAGGCATGTATTTTAAACTGAGGCATAGCCCAAAGCTCGTAAACTCAGGGTTCTGTCAGTCCATGTAGTAAAATCATGAAGCCCTACATATTTTTTCTTCTTTTGGTATAAGTTTCGG
20.
Forward primer:GGGATGCCAATAAGATGGAA
Reverse primer:AGAGGCCATCTGAGACTGCT
Amplicons (see Fig. 1a):[yellow1/yellow2]
Sequence:
GGGATGCCAATAAGATGGAAAAAAAATCAAGGAGAATCGGAGAATAAAATAATACCTGCACAGTATATATATTTTGGGGGCATTGTTGAGGTTGTACTTGTGATCTAATATCCATTTTTAAGTGTTTTGTAGATATTAAAGTTAATTTGCATGCGTACATATATATGTATATATACACATATAATAAATCCAGATATACATGTATTATATATCTATGAATAATACACATACATAACACATGTATAATAGATACTCCTATTAATCAGTATTATCA[C/T]TTAGATTACTCAAGACCTGGAATATATATTTGGAACAAAAGTAAAATCCCTTATTGTGATTCTACTTGCCAGCTCCTTATTATGGTCAACGTCAGAAGCCTTTTCATGGGCCCAGCAGTCTCAGATGGCCTCT
21.
Forward primer:CCCACTTATGCATGAGAATAGGTA
Reverse primer:GGAATGCTCTATGCTGCTGG
Amplicons (see Fig. 1a):[blue1/blue2,blue3,blue4]
Sequence:
CCCACTTATGCATGAGAATAGGTAGTGTTTGCTTTTCTGTTCCTGTGTTAGCTTGCTGAGAATGACGGTTTCCACTTCATTCATGTCTCTGAAAAGGACATGAACTTATTCATTTTTGTGGCTGCATAGTATTCCATCGTGTATATGTGCCACATTTTCTTTATCCAGTCTATAATTGATGGGCATCTGGGTTTGTGCCATGTCTTTTTTGTGTGTAAATAGTGCTGCAATAAGCA[GCACTAGCAGCAATAA/TACATGT]GCAGTTTCCTTAGAGTAGAATGATTTACAATACTCTGGGTGTATACCACATAATGGGATTGCTGGGTCAAATGGTATTTCTGGTTATAGATCCTTGAGGAATCACCACACTGTCTTCCACAGTGATTGAACTAATTTATACTCCCACCAGCAGCATAGAGCATTCC
22.
Forward primer:TGTGCCACATTTTCTTAATCCA
Reverse primer:TTCCAGAATACCAAAATCAACA
Amplicons (see Fig. 1a):[red1,red2/red3,red4]
Sequence:
TGTGCCACATTTTCTTAATCCAGTCTATCATTGTTGGAGTTAATGGGTGCAGCACACCAACATGGCACATGTATACATATGTAACAAACCTGCACGTTGTGCACATGTACCCTAAAACTTAAAGTATAATAATAATAATAATAATAA[TAATAATAATAA/]TAATAATGAAAGAATTGCAATTCAATTTTAATGTTGATTTTGGTATTCTGGAA
23.
Forward primer:ATGGGCTTGTAATCCCACCT
Reverse primer:AACCTCTTTGGACACAACGG
Amplicons (see Fig. 1a):[blue1/blue2/blue3,blue4]
Sequence:
ATGGGCTTGTAATCCCACCTACTTGTGAGGCTGAGGAAGGAGAATCAATTGAACCCAGGGGGTGGAGGTTGCATTAAGCTGACTGCTCAACTGCACTCCAGCCTAGGTGAAAGAGTGA[//ATAATAATA]GATTCAATAATAATAATAATAATAATAATAATAATAATA[/ATA/]TAAAAAAACATTATTAAGCAGCATAGTGAGTAACATTGGTGTTACTGAGAATTTTTTATATTCATCATTTCACTTCTTCTAGCCTCAATGGAAATTGAAAAGGCTGAAATATGCTCTCTCTGATGCAAGGGGCACAATGGACATACTTCATTTCTTTCCGTTGTGTCCAAAGAGGTT
24.
Forward primer:TTTGGAACACAGTAAGAGGGG
Reverse primer:GTCCTGGCTAACACGGAGAA
Amplicons (see Fig. 1a):[yellow1/yellow2]
Sequence:
TTTGGAACACAGTAAGAGGGGAATGAGAAGAATGAGAATAGTAAAATAAACCATTATTGAAGAGATATACTGTTAATGATGTCCTCCACCAATACAACTTGTTTTTCTTTTTTTTTTTTTTGTTTTTTGAGATGGAGTCTTGCTCTATCG[C/T]CAGCCTGGAGTGCAGTGGACATCTCAGCCCACTGAAACCTCTGCCTCCCAGGTTCAAGTGATTCCTCTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGCACCCGCCAGTGCGCCCAGCTAATTTTTTTTGTATTTTTTTAGTAGAGATGGGGTTTCTCCGTGTTAGCCAGGAC
25.
Forward primer:TTCAAAACGGTTGTTTGATCC
Reverse primer:CTTCACAATTGGTCAAGCCT
Amplicons (see Fig. 1a):[red1,red3,red4/red2]
Sequence:
TTCAAAACGGTTGTTTGATCCTAGGCAAGAATTTATATTTGCATGCCTTCTTATAATGTTGTATTAAAAACACATTTTACCGTCCTTACACACCTTGTCTGGAAATGTACTTCCAGTGGTTTCAATTACATATTATCA[C/T]GGTAAATTTTAGCAATTTTAACTTTAATGTAAAGCCAGGTAAATTGTCCTAATTATGTGCTAGGAGCAGCCATAATTAAGGGTGTGTTTAGTTCCATTTTTCCTCAGGCTTGACCAATTGTGAAG
26.
Forward primer:TGCAATCAAGCAAGAAGTCAA
Reverse primer:GGGAGGGAGTGTGTGTCAAG
Amplicons (see Fig. 1a):[green1/green2,green3]
Sequence:
TGCAATCAAGCAAGAAGTCAAGATTAAGAAACTCACTCAAAACCGCTCAACTACATGGAAACTGAACAACCTGCTTATGAATGACTACTGGGTACATAACGAAATGAAGGCAGAAATGAAATGTTCTTTGAAA[C/G]CAGTGAGAACAAAGACACAACATACCAGAATCTTGGGGATACATTTAAGGCAGTGTGTAGAGGGAAATTTATAGCACTAAATGCCCACAAGAGAAAGCAGAAAAGATCAAAAATCGACAGCCTAACATCACAGTTAAAAGAACTAGAGAAGCAAAAGCAAACACATTCAAAAGTTAGCAGTAGGTAAGAAATAACTAAGATCAGAGCAGAACTGAAGGAGTTAGAGACACAAAAAAACCCTTCAAAAAATCAGTGAATCCAGGAGCTGGTTCTTTGGAAAGATCAACAAAATTGATAGACTGCTAGCAA[G/A]ACTAATAACGAAGAAAAGAGAGAAGAATCAAAGAGACACAATAAAAATGACAAAGGGGAGATCATGACTGATCCCACAGAAATAGAAACTACCATCAGAGACTACTAGAAACACCTCTAGGCAAATAAACTAGAAAATCTAGAAAAAATGGATAAATTCCTTGACACACACTCCCTCCC
27.
Forward primer:GCCACAGTGTGTTATGGTCCT
Reverse primer:CCAGTATGCACCACCACATC
Amplicons (see Fig. 1a):[yellow1/yellow2]
Sequence:
GCCACAGTGTGTTATGGTCCTCATTATTTGGATGAATAATTTGAATAATAAGTCAGCTTGTGACTTACCTAAATAAATCTTCTGAGTCCTAGTAAAGTGTTTTTCATTATATGAAATGCCAGCTATATAAAAGGAGATTAAAAGTACTAAGA[A/G]CTGTAAGTTCCACATTGATTATCATAGGCTAACCATGGGCCAGGCACGGTGGCACACACCCATAATCCCAGCACTTTGGGAAGCTGAGGCGGGTGGATCACTTGAGGTCTGGAGTTCGAGACCAGCCTGGCCAAGATGGTGAAGCCCCATCTCTACCAAAAATACAAAAAAATTGCCAGATGTGGTGGTGCATACTGG
28.
Forward primer:TGCTATCGTTGCGTGAAATG
Reverse primer:GGGCAAATAAAATAAACTGAAACAA
Amplicons (see Fig. 1a):[red1,red3,red4/red2]
Sequence:
TGCTATCGTTGCGTGAAATGTTTTGTAGATTATCCCTAGATCTTATTTTTCTTTTTAATCTTTTATTTTTCTGTACATTTGTCTTTGTTTTTATCTTGTTTCTCTTTATTTTTATTTACTTTCATTTTTTCTATTAATTTAATTTTACTTCATTTTTTATTTTTTGTTTTTTCATTATAGTCTCAATTTCAAATTTTCTAGATTTTTGT[T/C]GTTTGTAATTTTATTCAATTTCTTGTTTCTTTTATTTATTTCCATGCATATCTCTTTGTTATTTTATTTTTCTCTGCATTTCTTTTTTACTTTCACTTGTCTCTTCTTATTCTTAAAATTTTCTTATTTCTCAATTTTATTGTATTTACTGTTTATCTTCATCTTGATTTTATTTTCACTAATCTTAGTTATATTTTTCTTTACTTTTCTCTATTTTGTTTCAGTTTATTTTATTTGCCC
29.
Forward primer:ACATTCAAATTCGGGAAACA
Reverse primer:TGCGCATTAGTTGATGTGGT
Amplicons (see Fig. 1a):[yellow1/yellow2]
Sequence:
ACATTCAAATTCGGGAAACACAGAGAACACCACAAAGATAATCCTCAAGAAGAGCAACTCCAAGATTCATAATTGTGAGATTCACCAAAGTTGAAATGAAGGAAAAAATGTTAAGGGCAGCCAGAGAGAAGGTGGGGTTACCCACAAAGGGGGGCCCATCAGACTAACAGCAGATCTCTTGGCAGAAACTCTACAAGCCAGAAGAGAGTGGGGGTCAACATTCAACATTCTTA[G/A]AGAAAAGAATTTTCAACCCAGAATTTCATATCCAGCCAAATTAATCTTCATAAGTGAAGGAGAAATACAATCCTTTACAGACAAGCAAATACTGAGAGATTTTGTCACCACAAGGCCTGCCCTACAAGAGCTCCTGAAGGAAGCATTAAACATGGAAAGGAACAACCAGTACCAGCCACTGTAAAAACATGACAAATTGTAAAGACCATCGAGGCTAGGAAGAAACCACATCAACTAATGCGCA
30.
Forward primer:GCAACATGACATCACTGCAA
Reverse primer:TGACCCAGCACTGTTGGAC
Amplicons (see Fig. 1a):[yellow1/yellow2]
Sequence:
GCAACATGACATCACTGCAAGAGACAAAACAGCACATAGCCTTCTTGTGACTGTATTTTGCTGACAGTCCATGAGCAGATAGCCTGAACTCAGCAGTGCTGTTCCCTTGGGAAACACACACACACACACACACACACACACACACACACACACA[CACA/]CGAGTTGGTGGTTTTCTGCCTCCACCCCCACCCCAACACACACACGGGTTGGTGGTTGTGCTGCCCGGAGCCTCCAGTCCGCGAGTGTGAAGAACGGACCAGATGGGTCCAACAGTGCTGGGTCA
31.
Forward primer:TCATGCCAATTACCAAGGTG
Reverse primer:CATAGGAGCTGGACAATGGC
Amplicons (see Fig. 1a):[green1/green2,green3]
Sequence:
TCATGCCAATTACCAAGGTGAAGTTTGTCTACTGTTCTTCCTAAGCCTTGCCCACAAGGGGGATTTGCATATATCACTGAAACCAGCATCCAGGTGAAGAGACTCTTCTCCCACAGTCCTGCCCACAGGTAGGATTGTGACATTTCACTGGACCAGCACCCACACA[A/G]ATGATGTGACTTTCCTTTCTTCTCCCTGCCAGAGGTGATACTGTTCCATATACCTGAGACCAGACCGAAACCCTAATAATGACAATTGTACCTGGAGCCAGGACACATGCAAGGTGGTGACTTTCATTTCTTAACCTTTCCACAGTTGTTTTTGTGACATATGCCATTGTCCAGCTCCTATG
32.
Forward primer:GCTGAAGCACTGGGTTGAAG
Reverse primer:ATGTCAAAAGCAAAACCCTAGT
Amplicons (see Fig. 1a):[blue1,blue2/blue3/blue4]
Sequence:
GCTGAAGCACTGGGTTGAAGCTTTTGAATTTTAAGTATGCATGTTTATTTTTGAAACTCAGAATTATGAGAGTAAAAAAATTACTATATATATGCATATATAGTATATATGTATACACAGTAACCGTGATATATATATATATATATATATA[/TATATA/TATATATATATATA]ATCACAATATTCTAATGTTTATTGCCAAGTAGAGTTCTTCTTTATAAAGGTGATATCACCTATAATTAGAAAATACAAATTAAGAGGAAAAAACACTAGGGTTTTGCTTTTGACAT
33.
Forward primer:CGTCACCTCTCCTAACAGGC
Reverse primer:CACTGCCTTTTGAGAAACCA
Amplicons (see Fig. 1a):[yellow1/yellow2]
Sequence:
CGTCACCTCTCCTAACAGGCAATTTCCTAAAAATATATTGAAATATAAAATATCTGAAGATTGAGACATTACTCATCTTCCAGTTTCAAGTGAAATATACACAAGAAATCAGGAGGTTATATGTAAAGTAAAAATATTATAAATAAAAA[/A]TATTTTAGAAGGCCACAAAATAAGAAGCTATGTCACAGGAAACAGAGAAGTTAAAGTTTTTCCTTAAGTGGAAAAGAACATATCTCATGCTAAAAGTAATAGGCACCAGAGGCAATAAGTTGCTGCCACCTTGATAATTACAGGTTTTTCAAAATATATTGAAATAAAGCTTATTAAAAATACAGAAACAATCACTGGCCTTTCTGACTATATAAAATATTAAAAATAATGGTTTCTCAAAAGGCAGTG
34.
Forward primer:GACCAAGAGGAAAACGATGG
Reverse primer:GACTCAAATTGGGAAGATAATAACC
Amplicons (see Fig. 1a):[yellow1/yellow2]
Sequence:
GACCAAGAGGAAAACGATGGTTTCAGTAGCCTTATGACAACATAGCCCTCCATTTTGTATGATTGTGATTTATAACATGAAGGCAGGGTTTTACTGTACATTTTATGTTTGAAAGTCATATCCCAGGCTTTAGTACTTAGGGACTTTGAAGTTCACACAATGTCGTGTTCAGTGCCCTCAGGTGCACCTGTACCATAGATAAGCTTCTGCATTGATAAACGTCTTT[C/T]TGGAAAGGTAACTGTGAAACTTGTATTTAATTGTTCTTATTCTCAGGTATCAACTTGAATTAATGGATGACTACATCATTAGAACTAACTGACTGATTGAAGAGCAAAGGAAGAATAAAGGTGAGGTTTGGGTGGGGTAAGAAGCCGTAGTTTTCAGTTTTGATACAGGTTATTATCTTCCCAATTTGAGTC
35.
Forward primer:TCAAGCCTGGGTGGTAGAGT
Reverse primer:TGTCACGGTATAACATCTCCAG
Amplicons (see Fig. 1a):[yellow1/yellow2]
Sequence:
TCAAGCCTGGGTGGTAGAGTAAGACCCTGTCTCAAAAAAAAAAAAAAAAAAAGAAAAATTACTAAGCAAAATAAGACATGTGAAGGATCATGTCAAAGGTAAGAAAAATTAGGGGAACATTAAAAGCTTTCTTCCCAAGCCACTAAATCAACTTGACTAA[C/A]AAAATTACCACTTGATTTAGCATTAGAAAATTACATTACATATCAAACATAAACCCATTAATCAAATACTAAAGAAATTTCTGAGTTAAATGGTATAATGTTAGCTTATGCCGGAGCTGACCTTGAAAGATTGTTCAAATATGGCTCAGTGTGATTGAAAGTTCTGTGTGAATATGTTTTTGGAAAGATCCAATAGCAACATCTTAGTGTATGTTTTTGAAATAAAATGTATCTGAGTAGCAGCAAAGTTATTCTTAAATTTCCATTTTATAGCTGGAGATGTTATACCGTGACA
Supplementary InformationKuroda-Kawaguchi et al.1