Valentine VLASSOV, Academician RAS

DAY 1: Monday, May 13

11:00 – 11:30Registration

11:30 – 12:00Opening ceremony

Valentine VLASSOV, Academician RAS

Nikolay LYAKHOV, Academician RAS

Patrick CURMI, APILIFE Coordinator

Alain KROL, LIA Coordinator

Olga LAVRIK, APILIFE Coordinator, RAS Corresponding Member

12:00-13:15 Session 1.

Chairpersons: Marc Mirande and Andre Dietrich

12:00-12:25 The tRNA3Lys packaging complex involves association of human mitochondrial LysRS with the polyprotein GagPol from HIV-1: a new pharmacologic target ?

J. Dias, L. Kobbi, A. Nail, A. Szklarz, M. Comisso andMarc MIRANDE* (Centre de Recherche de Gif)

12:25-12:50 Manipulating Mitochondrial Genetics

D. Mileshina, F. Weber-Lotfi, P. Boesch, N. Ibrahim, M. Koulintchenko, G. D'Souza, V. Saxena, V. Tarasenko, Y. Konstantinov, R. Lightowlers and Andre DIETRICH* (Institut de Biologie Moleculaire des Plantes)

12:50-13:15 Mitochondrial DNA Import Mechanisms

Frederique WEBER-LOTFI*, N. Ibrahim, M. Koulintchenko, D. Mileshina, Y. Konstantinov, R. Lightowlers and A. Dietrich

*(Institut de Biologie Moleculaire des Plantes)

13:15-13:40 Specificity of DNA Import into Isolated Mitochondria of Plants and Mammalians

Milana KOULINTCHENKO*, N. Ibrahim, E. S. Klimenko, Yu. M. Konstantinov, F. Lotfi, A. Dietrich

*(Siberian Institute of Plant Physiology and Biochemistry)

13:40-15:00Lunch

15:00-16:15 Session 2.

Chairpersons: Olga Lavrik and Patrick Curmi

15:00-15:25 Influence of multifunctional protein YB-1 on the enzymes involved in the repair of AP-sites in DNA

Pavel PESTRYAKOV*, E. Fomina, S. Khodyreva, D. Zharkov, L. Ovchinnikov, P. Curmiand O. Lavrik

*(ICBFM SB RAS)

15:25-15:50 Impact of the YB-1 Protein on Recognition and Repair of DNA Damages

Dmitry KRETOV*, L.P. Ovchinnikov, O. Lavrik, V. Joshi and P.A. Curmi

*(Université d’Evry val d’Essonne)

15:50-16:15 Biomolecular Interactions on Surface Investigated at High Resolution by AFM

Loïc HAMON*, P.A. Curmi, V. Joshi, M. Sukhanova, O. Lavrik and D. Pastre

*(Université d’Evry val d’Essonne)

16:15-16:40Coffee Break // Poster Session

16:40-17:55 Session 3.

Chairpersons: Olga Fedorova and Jean-Christophe Amé

16:40-17:05 Active DNA Demethylation and Epigenetic Reprogramming: Insight into Mechanism of Recognition of 5-Hydroxymethyluracil by Methyl-Binding Domain Protein 4

Inga GRIN*,S. Moréra, A. Vigouroux, S. Couvé, V. Henriot, M.Saparbaev and A.A. Ishchenko

*(Institute de Cancérologie Gustave Roussy)

17:05-17:30 The Mechanism of DNA Lesion Search by Human 8-Oxoguanine DNA Glycosylase

Nikita A. KUZNETSOV*, A.A. Kuznetsova, A.A. Ishchenko, M.K. Saparbaev and O.S. Fedorova

*(ICBFM SB RAS)

17:30-17:55 The Kinetic Mechanism of Human Apurinic/Apyrimidinic

Endonuclease 1 in NIR Pathway

Nadejda TIMOFEYEVA*, V.V. Koval, A.A. Ishchenko, M. Saparbaev and O. Fedorova

*(ICBFM SB RAS)

18:30 Welcome reception (Restaurant “Vkusnyi centr”)

DAY 2: Tuesday, May 14

10:00-11:15 Session 4.

Chairpersons: Valérie Schreiber and Svetlana Khodyreva

10:00-10:25 PARG Facilitates Cellular Recovery From Prolonged Replication Stress

G. Illuzzi, E. Fouquerel, J.-C. Amé, F. Dantzer andValérie SCHREIBER* (Université de Strasbourg)

10:25-10:50 Interactions of HMGB1 and HMGB2 Proteins with Apurinic/Apyrimidinic Sites in DNA

Ekaterina ILINA*, M.Kutuzov, D. Modestov, S. Agdauletova, O. Lavrik and Khodyreva S.N. *(ICBFM SB RAS)

10:50-11:15 Comparative Analysis of Interaction of Human and Yeast DNA Damage Recognition Complexes with Damaged DNA in Nucleotide Excision Repair

Nadejda RECHKUNOVA*, Y. Krasikova, E. Maltseva, P. Pestryakov, I. Petruseva and O. Lavrik*(ICBFM SB RAS)

11:15-11:45 Coffee Break // Poster Session

11:45-13:00 Session 5.

Chairpersons: Elena Rykova and Loïc Hamon

11:45-12:10 Interaction of Ddc1 and RPA with Single-Strand/Double-Strand DNA Junctions In Cell Free Extracts of Saccharomyces Cerevisiae

Maria SUKHANOVA*, C.D’Herin, P.A van der Kemp., S. Boiteux and O.I.Lavrik *(ICBFM SB RAS)

12:10-12:35 Circulating DNA for Cancer Diagnostics: Concentration, Epigenetic Characteristics, Association with Blood Complexes and Cells

Elena RYKOVA*, E.S. Morozkin, E.M. Loseva, A.A. Ponomaryova, A.M. Kurilshikov, I.V. Morozov, I.S. Zaporozhchenko, K.Y. Kapitskaya, T.L. Azhikina, L.O. Bryzgalov, E.V. Antontseva, T.I. Merkulova, N.V. Cherdyntseva, V.V. Vlassov, P.P. Laktionov *(ICBFM SB RAS)

12:35-12:55 Intracellular Targets of Immunoinhibiting Extracellular DNA

Anna CHEREPANOVA*, T. Duzhak, V. Vlassov, P. Laktionov

*(ICBFM SB RAS)

13:00-14:30Lunch

14:30–15:45 Session of LIA 1.

Chairpersons: Olga Dontsova and Eric Westhof

14:30– 14:55 Recoding UGA as Selenocysteine: Idiosyncratic and Shared Factors for mRNP Assembly and Translation

Alain KROL(University of Strasbourg)

14:55– 15:20 Eukaryote-Specific Features of mRNA Path in the Human Ribosome

Dmitry GRAIFER*, Yu.S. Khairulina, D.E. Sharifulin, A.G. Ven’yaminova, G.G. Karpova*(ICBFM SB RAS)

15:20– 15:45 Lys53 of Ribosomal Protein L36al is Close to the CCA End of a tRNA at the P/E Hybrid Site on the Human Ribosome

Konstantin BULYGIN*, S.Baouz, A.Woisard, C.Hountondji, G.Karpova*(ICBFM SB RAS)

16:00- 22:30 Free time, cultural programme

DAY 3: Wednesday, May 15

10:00– 11:15Session of LIA 2.

Chairpersons: Galina Karpova and Alain Krol

10:00– 10:25 “Unusual” RNA Base Pairs in Recognition and Decoding

Eric WESTHOF*(University of Strasbourg)

10:25– 10:50 New features of telomerase RNA processing

M. Rubtsova, A. Malyavko, D. Vasilkova, M.Zvereva, E.Smekalova, V. Prassolov, E.Westhoff and Olga DONTSOVA*

*(Department of Chemistry and Belozersky Institute Lomonosov Moscow State University)

10:50– 11:15 New players in recognition of AP sites: Implication in DNA repair

Svetlana KHODYREVA*, E.Ilina, M.Kutuzov, M.Sukhanova, J.-C.Amé, V.Schreiber and O.Lavrik *(ICBFM SB RAS)

11:15-11:45 Coffee Break // Poster Session

11:45– 13:00Session of LIA 3.

Chairpersons: Marina Zenkova and Dmitry Graifer

11:45-12:10 Identification of recombinant products of non-enzymatic reaction of RNA cleavage/ligation

Marina ZENKOVA*, Ya. Staroselec, E. Brenner, V. Vlassov

*(ICBFM SB RAS)

12:10-12:35 Nucleic acid based anticancer therapeutics

T. Kabilova, Elena CHERNOLOVSKAYA*, M. Zenkova and V. Vlassov *(ICBFM SB RAS)

12:35-13:00 Identification of molecular targets of RNase A in antitumor therapy

O. Patutina, Nadejda MIRONOVA*, E. Brenner, A. Kurilshikov, V. Vlassov, M. Zenkova *(ICBFM SB RAS)

13:00-14:30 Lunch

14:30-14:55 Ubiquitin musical chairs: Juggling multiple molecular signals Institute of Technology between shuttles and receptors

Michael Glickman(Bio-Rad)

14:55-15:20 SPR: applications in biomolecular interaction analysis

Anna SYCHEVA (Bio-Rad)

15:20-16:00 CONCLUDING REMARKS

Patrick CURMI, Olga LAVRIK, Alain KROL, Valentin VLASSOV

19:00 Gala Dinner (Restaurant “House of Scientists”)