UC Irvine Environmental Health & Safety: Example

UC Irvine Environmental Health & Safety: Example

PI Name:______

Standard Operating Procedures for PHORBOL ESTERS and PHORBOLS

#1 /

Process

Handling and weighing pure chemical to prepare administration dosages for induction of tumors.
#2 /

Hazardous Chemicals/Class of Hazardous Chemicals/Signs & Symptoms of Exposure

Phorbol and phorbol esters including phorbol myristate acetate. Very Toxic to Harmful. May be fatal if swallowed. Readily absorbed through skin. Irritating to skin, eyes, and mucous membranes. Phorbol and phorbol esters as a class are some of the most potent tumor promoters known to man. Some phorbol esters enhance tumor promotion of known carcinogens ten fold. Experimental evidence indicates PMA to be carcinogenic, teratogenic, and mutagenic.
#3 /

PPE - Personal Protective Equipment

Wear two layers of chemical resistant glove like disposable nitrile, safety goggles, and laboratory coat. Material is readily absorbed through skin.
#4 /

Engineering/Ventilation Controls

Use with safety shower and eyewash nearby. Use only in a chemical fume hood until diluted into liquid.
#5 /

Special Handling Procedures and Storage Requirements

Read Material Safety Data Sheet prior to use.
Use extreme caution when handling these compounds with carcinogenic compounds.
Do not breathe dusts. Do not get in eyes, on skin, on clothing.
Keep in tightly closed container. Store in a cool dry place at -20°C.
Protect from light. Avoid strong bases and strong oxidizing agents.
Order as small as quantity needed.
Wash thoroughly after handling.
#6 /

Spill and Accident Procedures

If outside the hood, evacuate unnecessary personnel, contact EH&S as self-contained breathing apparatus is required. If in hood, then gently sweep up, place in a bag and hold for waste disposal. Avoid raising dust. Ventilate area and wash spill site after material pick up is complete.
#7 /

Waste Disposal

Dispose of all items contaminated with phorbols as hazardous waste through EH&S, including empty source vials & containers.
#8 /

Approval Required

Supervisor approval required for first use.
#9 /

Decontamination Procedures

After removing bulk material via absorption or gentle sweeping, wash surfaces with soap and water.
#10 /

Designated Area

Please identify.

For more information relevant to completing this form, review thoroughly Chemical Hygiene Plan: Section 4; Section 5; Guidelines for SOP Development; Special Handling Procedures for Common PHS; Chemical specific MSDS.

Training Acknowledgement: I have read, asked questions, and understand the hazards of and safe working procedures for the activity/materials described herein.

NAMEDATE

These safety training resources, prepared solely for the use of the Regents of the University of California, were provided by a variety of sources. It is your responsibility to customize the information to match your specific operations. Neither the University of California nor any of its employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, or represents that its use would not infringe privately owned rights. Reference herein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise, does not necessarily constitute or imply its endorsement, recommendation, or favoring by the University of California. The views and opinions of authors expressed herein do not necessarily state or reflect those of the University of California, and shall not be used for advertising or product endorsement purposes.

Chemical Hygiene Plan, 10/12/2018