Transgenic and Gene Targeting Core (Tgtc)

TRANSGENIC AND GENE TARGETING CORE (TGTC)

Construct Microinjection Service Form

Investigator: / Submission Date:
Production Protocol#: / Contact Person:
Experimental Protocol#: / Contact Phone:
Speed Type #: / Contact Email:
SDRC Member? CCTSI Member? eRA Commons Name:

Submission Information:

DNA Preparation:The Investigator is responsible for purifying and linearizing 30-50ug of DNA according to the protocol provided. Please provide DNA at 1ug/µl concentration in sterile TE buffer (10mM Tris-HCl, pH7.6, 1mM EDTA) clearly labeledwith PI’s name, construct name, concentration, and date.

DNA DROP-OFF Location:RC-1 North, 8th floor, room P18-8403E. Place samples in the Kenmore Elite refrigerator (outside Koch Lab) labeled “Transgenic Core”. Please see receptionist, room P18-8130(admin office) if you do not have access to this lab area. Please send an email notification to Makeamouse before dropping off DNA.

All samples submitted will go through quality control and assurance process before initiating the experiment. For more information and questions regarding DNA preparations or ES targeting process please contact core personnel at Makeamouse.

Will you require DNA Purification: Yes NoBAC Construct: Yes No

Label sample with construct name and PI. Complete the following information:

Construct Name: / Construct Abbreviation (10 char):
Construct preparation date: / Amount of purified DNA supplied (1 µg min) : µg
Volume of DNA (50 µl min): µl / Concentration (15 ng/µlmin): ng/µl
A260/A280 ratio: / Construct size: Kb

If submitting dna samples for purification, please provide 5 µg of digested plasmid for purification and 1 µG each of undigested and linearized dna for quality control

please include a gel picture AND A PLASMID MAP for all dna submissions

If submitting BAC DNA please contactMakeamouse for Details.

Please provide short description of your construct. Include any promoter/enhancer descriptions:
In what strain should we develop your transgenic model? C57BL/6 ; FVB ; Other; strain?
NOTE: If you selected ‘other’, please contact the core lab at 4-3012 or Makeamouse

Following preparation and verification of the construct, this application will be transferred to the Transgenic and Gene Targeting Core for scheduling and microinjection.

Insert photograph of gel here

Show size marker, undigested vector, linearized vector, construct digested to release transgene, purified transgene (indicate enzymes used for digestions)

Construct approved for injection: Yes No

Received __ __/_____/_____ by ______(initials) for Transgenic and Gene Targeting Core.

Injection Date: __ __/_____/_____

appl PNI injection.docxjwa/pjk 7/5/2011 v2.5