Supplementary Data

Transcriptome analysis of Sf9 cells infected with AcMNPV or AcMNPV-BmK IT

Lili Wei#1, Leixi Cao#1, Yanyan Miao#1, Shuju Wu#2, Shumei Xu1, Ruisheng Wang1, Jun Du1, Aihua Liang1Yuejun Fu*1

1Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China;

2School of Life Science and Technology, HarbinNormalUniversity, Harbin, 150025, China.

# These authors contributed equally to this work.

*Correspondence and requests for materials should be addressed to Y. F. (email:.)

Tel.: +86 351 7016125;

Fax: +86 351 7011499.

Supplementary Figure 1. Analysis ofthree specimens(a) The species distribution of annotated genes in NR database. (b) Venn diagram for database Nr，KEGG，Swissprot and KOG. (c) Correlogram of three specimens reflected both the Euclidean distance between specimens. (d) Cluster Dendrogram of three specimens.

Supplementary Figure 2. KOG categories. The most abundant groups are General function prediction only and Signal transduction mechanisms.

Supplementary Table 1 Primers for qPCR

Primer name / Primer sequence (5'to3')
Acorf30 F Primer / GGGCGAAGAGGGATTGTT
Acorf30 R Primer / GCGATAAGTGTTGGCATTGA
Acorf109 F Primer / TGCCCGTTTCAGATTCAAGT
Acorf109 R Primer / GGCACGTACACGATCAAGTTT
TRAF6 F Primer / GCACCGCCACTAAACAATACT
TRAF6 R Primer / TCGCTATCACTATGTCCTGCTC
α-amylase F Primer / CGTTATGCTGATGCCTGGT
α-amylase R Primer / TGATTGGGTCGTCTGTGTTG
△9-AcDt F Primer / TACTCCGAAACCGATGCTG
△9-AcDt R Primer / AAGTCGCTCAGGTCAATGGT
Tret1 F Primer / GCCAACACTTTCTGCTTCAAC
Tret1 R Primer / CGTCTTCACTGCTGCTCTCA
FAR F Primer / ACGAAGTATTGGGCGAGAGA
FAR R Primer / CGTGTAGCAGTTGGGATGC
5NUC F Primer / GCAACTTCCCGTGGCTAAT
5NUC R Primer / TTTCCAACCATTCCTGTTCG
PCNA F Primer / TTTGAAGAAGGTGTTGGAAGC
PCNA R Primer / TCTGAGTGTGAGGGAAACCA
Ecm29 F Primer / GAGACAGAGACGGGAACAGC
Ecm29 R Primer / CGTTGGGTAGGACTTTGAACA
Xandh F Primer / TTGCGAGTTCCAGTATGAGTG
Xandh R Primer / CGGAGTCGTTGTAAGAGCAG

Supplementary Table 2 Pollution test of C

Species / Reads_number
Spodoptera frugiperda / 18490
Bombyx mori / 3522
Papilio xuthus / 2353
Spodoptera exigua / 1955
Spodoptera litura / 1691
Helicoverpa armigera / 1445
Papilio polytes / 838
Heliothis virescens / 581
Manduca sexta / 462
Hemileuca sp. L6 / 452
Spodoptera littoralis / 400
Calesia nigriventris / 365
Trichoplusia ni / 331
Spodoptera triturata / 323
Antheraea pernyi / 205

Supplementary Table 3 Pollution test of AT

Species / Reads_number
Spodoptera frugiperda / 18504
Bombyx mori / 2988
Spodoptera exigua / 2021
Papilio xuthus / 1691
Spodoptera litura / 1589
Helicoverpa armigera / 1355
Papilio polytes / 978
Heliothis virescens / 609
Manduca sexta / 444
Spodoptera littoralis / 404
Trichoplusia ni / 317
Hemileuca sp. L6 / 232
Calesia nigriventris / 166
Plutella xylostella / 159
Spodoptera triturata / 154

Supplementary Table 4 Pollution test of ABT

Species / Reads_number
Spodoptera frugiperda / 19849
Bombyx mori / 2988
Spodoptera exigua / 2138
Papilio xuthus / 1714
Spodoptera litura / 1631
Helicoverpa armigera / 1403
Papilio polytes / 975
Heliothis virescens / 627
Manduca sexta / 470
Spodoptera littoralis / 424
Trichoplusia ni / 364
Hemileuca sp. L6 / 203
Spodoptera triturata / 183
Mythimna separata / 165
Heliconius melpomene cythera / 157

Supplementary Table 5 Results of splicing

All_num / >=500bp / >=1000bp / N50 / N90 / Max_len / Min_len / All_len / Mean_len
Transcript / 98575 / 51146 / 35319 / 3307 / 484 / 29268 / 201 / 138527336 / 1405.3
Unigene / 61269 / 20282 / 10431 / 1536 / 273 / 29268 / 201 / 46427777 / 757.77

Supplementary Table 6 Top ten up-regulated DEGs in AcMNPV and AcMNPV-BmK IT treatment compared to control

Gene_ID / Log2Foldchange / fold
Top ten up-regulated genes(ABT-AT)
comp758_c0_seq2 / 4.268866845 / 19.29
comp90723_c0_seq1 / 3.927829927 / 15.24
comp77282_c0_seq1 / 2.721379049 / 6.59
comp53278_c0_seq1 / 2.605901832 / 6.11
comp6341_c0_seq2 / 2.342867426 / 5.06
comp36889_c0_seq1 / 2.176217557 / 4.53
comp17326_c0_seq1 / 2.101859327 / 4.29
comp164339_c0_seq1 / 2.071565404 / 4.2
comp18016_c0_seq2 / 2.020939331 / 4.06
comp161156_c0_seq1 / 1.927829927 / 3.81
Top ten up-regulated genes(ABT-C)
comp230762_c0_seq1 / 4.467760967 / 22.16
comp269608_c0_seq1 / 4.467760967 / 22.16
comp330134_c0_seq1 / 4.342230085 / 20.25
comp16011_c0_seq1 / 4.15581696 / 17.88
comp281282_c0_seq1 / 3.789689062 / 13.83
comp9909_c0_seq1 / 3.583238184 / 11.96
comp20833_c0_seq1 / 3.467760967 / 11.08
comp15238_c0_seq1 / 3.105190887 / 8.63
comp13573_c0_seq2 / 3.092251832 / 8.51
comp65590_c0_seq1 / 3.052723467 / 8.28
Top ten up-regulated genes(AT-C)
comp22685_c0_seq1 / 15.57199291 / 48644.87
comp21941_c0_seq1 / 14.47034499 / 22693.63
comp20216_c0_seq1 / 13.27905237 / 9946.68
comp22685_c1_seq1 / 12.88578671 / 7590.61
comp7187_c0_seq1 / 4.71984013 / 26.35
comp351_c0_seq1 / 4.562298853 / 23.59
comp78706_c0_seq1 / 4.505715325 / 22.78
comp135033_c0_seq1 / 4.446821636 / 21.86
comp15246_c0_seq1 / 4.385421091 / 20.97
comp163005_c0_seq1 / 4.385421091 / 20.97

Supplementary Table 7 Top ten down-regulated DEGs in AcMNPV and AcMNPV-BmK IT treatment compared to control

Gene_ID / Log2Foldchange / fold
Top ten down-regulated genes(ABT-AT)
comp21335_c0_seq1 / -10.62201489 / 0.00064
comp406273_c0_seq1 / -4.837041664 / 0.035
comp487360_c0_seq1 / -4.786415591 / 0.036
comp22587_c0_seq1 / -4.502622625 / 0.044
comp355399_c0_seq1 / -4.438492287 / 0.046
comp264679_c0_seq1 / -4.371378092 / 0.048
comp889_c0_seq1 / -3.979060669 / 0.063
comp248061_c0_seq1 / -3.56402317 / 0.085
comp248285_c0_seq1 / -3.56402317 / 0.085
comp320794_c0_seq1 / -3.56402317 / 0.085
Top ten down-regulated genes(ABT-C)
comp264598_c0_seq1 / -4.439129629 / 0.046
comp192269_c0_seq1 / -4.204664375 / 0.054
comp208225_c0_seq1 / -4.204664375 / 0.054
comp124105_c0_seq1 / -3.741692399 / 0.075
comp248285_c0_seq1 / -3.576633153 / 0.084
comp262118_c0_seq1 / -3.576633153 / 0.084
comp30454_c0_seq1 / -3.517739464 / 0.087
comp46677_c0_seq1 / -3.447591208 / 0.091
comp324220_c0_seq1 / -3.439129629 / 0.092
comp129944_c0_seq1 / -3.232678751 / 0.11
Top ten down-regulated genes(AT-C)
comp240085_c0_seq1 / -4.661702821 / 0.04
comp246670_c0_seq1 / -4.46006896 / 0.045
comp181268_c0_seq1 / -4.308065867 / 0.05
comp411529_c0_seq1 / -4.308065867 / 0.05
comp192269_c0_seq1 / -4.225603706 / 0.053
comp50038_c0_seq1 / -3.945495787 / 0.065
comp30454_c0_seq1 / -3.71221505 / 0.076
comp324894_c0_seq1 / -3.386068379 / 0.095
comp3520_c0_seq2 / -3.386068379 / 0.095
comp356690_c0_seq1 / -3.386068379 / 0.095

Supplementary Table 8 Go and pathway enrichment of DEGS

Compare / DEGs / GO / Pathway
P<=0.05 / FDR<=0.05 / P<=0.05 / FDR<=0.05
ABT,AT / 173 / 76 / 17 / 3 / 0
ABT,C / 213 / 120 / 0 / 12 / 7
AT,C / 611 / 108 / 16 / 9 / 0

1