Title: Tissue-specific expression of telomerase reverse transcriptase gene variants in Nicotiana tabacum

Journal: Planta

Authors: Jana Fišerová Jurečková, Eva Sýkorová, Said Hafidh, David Honys, Jiří Fajkus, Miloslava Fojtová

Correspondence: M. Fojtová

Central European Institute of Technology (CEITEC) and Faculty of Science, Masaryk University Brno, Czech Republic

Table S1: Sequences of primers used for specific amplification of TERT gene variants and quantification of telomerase activity.

PRIMER / SEQUENCE (5´→ 3´) / METHOD
9ex Fw / GTGCTGGATGATCTCAATTTGGAG / forward primer for amplification of all TERT gene variants
9ex Rev_Ct / GAAGACAAGACCTGGCATTAGCAG / reverse primer for amplification of Ct variant of the TERT gene
9ex Rev_Cs / GGAAGATAAGACCTAGCATTGGCG / reverse primer for amplification of Cs variant of the TERT gene
9ex Rev_C / CAAGAATGCCATGCAATGAGCC / reverse primer for amplification of C variants of the TERT gene
9ex Rev_D / GAAGACAAGACCTGGCATTGACG / reverse primer for amplification of D variant of the TERT gene
actin Fw / CTGGATTTGCTGGTGATGAT / forward primer for amplification of actin reference gene
actin Rev / CYCTCTTGGATTGAGCTT / reverse primer for amplification of actin reference gene
EF1 Fw / TGAGATGCACCACGAAGCTC / forward primer for amplification of EF1 reference gene
EF1 Rev / CCAACATTGTCACCAGGAAGTG / reverse primer for amplification of EF1 reference gene
AtTERT 1ex Fw / CCGATGATCCCATTCACTACCGTAAACT / forward primer for amplification of A. thalianaTERT gene
AtTERT 1ex Rev / TCTCTGTGACCACCAAGATGTTGGAGA / reverse primer for amplification of A. thalianaTERT gene
ubq Fw / AACGGGAAAGACGATTAC / forward primer for apmlification of A. thaliana ubiquitine reference gene
ubq Rev / ACAAGATGAAGGGTGGAC / reverse primer for apmlification of A. thaliana ubiquitine reference gene
TS21 / GACAATCCGTCGAGCAGAGTT / qTRAP
TELPR / CCGAATTCAACCCTAAACCCTAAACCCTAAACCC / qTRAP
up_Ct Fw / GATTTTCATTACTGTTGTCTTTTACGG / forward primer for ChIP – amplification of the TERT_Ct upstream region
up_Cs Fw / GTCGATTTTTCAATACTGTCGAC / forward primer for ChIP – amplification of the TERT_Cs upstream region
1ex Rev / ACCGGTGGAACTCTCCGTCTCT / reverse primer for ChIP