Title: Genes, Color, and Geography: the Evolutionary Enigma of the Pygmy Angelfishes From

DiBattista, J.D., Randall, J.E., Newman, S.J., and Bowen, B.W. (2014) Round herring (genus Etrumeus) contain distinct evolutionary lineages

coincident with a biogeographic barrier along Australia’s southern temperate coastline. Marine Biology. doi:10.1007/s00227-014-2516-5.

Electronic Supplementary Material

Appendix S1 Material examined

Museum Voucher / Tissue ID / SL / Sample location / Sample date / Collector / GenBank Acc.#
COI, Cyt b
AMS I. 17313-004 / n/a / 134.5 / Off Brunswick, NSW (S 28˚ 32’, E 152˚ 37’) / August 1973 / T. Wongratana, NSW State Fisheries / n/a
AMS I. 17313-004 / n/a / 129.0 / Off Brunswick, NSW (S 28˚ 32’, E 152˚ 37’) / August 1973 / T. Wongratana, NSW State Fisheries / n/a
AMS I. 17313-004 / n/a / 106.0 / Off Brunswick, NSW (S 28˚ 32’, E 152˚ 37’) / August 1973 / T. Wongratana, NSW State Fisheries / n/a
AMS I. 17313-004 / n/a / 107.5 / Off Brunswick, NSW (S 28˚ 32’, E 152˚ 37’) / August 1973 / T. Wongratana, NSW State Fisheries / n/a
AMS I. 17313-004 / n/a / 112.0 / Off Brunswick, NSW (S 28˚ 32’, E 152˚ 37’) / August 1973 / T. Wongratana, NSW State Fisheries / n/a
AMS I. 17313-004 / n/a / 112.0 / Off Brunswick, NSW (S 28˚ 32’, E 152˚ 37’) / August 1973 / T. Wongratana, NSW State Fisheries / n/a
WAM P28332-001 / n/a / 146.0 / 100 M of N Mole, Fremantle, Western Australia (S 32˚ 03’, E 115˚ 44’) / July 1984 / N. Sinclair / n/a
WAM P28332-001 / n/a / 146.0 / 100 M of N Mole, Fremantle, Western Australia (S 32˚ 03’, E 115˚ 44’) / July 1984 / N. Sinclair / n/a
CSIRO C2647 / n/a / 180.0 / Oyster Harbour, Albany, Western Australia (S 34˚ 58’, E 117˚ 57’) / November 1954 / n/a / n/a
CSIRO CA182 / n/a / 133.5 / Jervis Bay, NSW (S 85˚ 00’, E 150˚ 45’) / July 1977 / I.S.R. Munro / n/a
CSIRO CA183 / n/a / 131.0 / Jervis Bay, NSW (S 85˚ 00’, E 150˚ 45’) / July 1977 / I.S.R. Munro / n/a
WAM P32528.001 / n/a / 182.5 / Rottnest Island, Perth Canyon, Western Australia (S 31˚ 57’, E 115˚ 08’) / February 2004 / G.I. Moore, “RV Southern Surveyor” / n/a
WAM P32528.001 / n/a / 183.0 / Rottnest Island, Perth Canyon, Western Australia (S 31˚ 57’, E 115˚ 08’) / February 2004 / G.I. Moore, “RV Southern Surveyor” / n/a
CSIRO C1934 / n/a / 133.0 / Lake Illawarra, NSW (S 34˚ 32’, E 150˚ 50’) / January 1940 / I.S.R. Munro / n/a
AMS I.45555-001 / Eter113 / 119.5 / 3 miles off Clarence River, NSW (S 29˚ 25’ 50”, E 153˚ 25’ 25”) / July 2011 / “Commercial Prawn Trawler” / KM116360,KM116286
AMS I.45555-001 / Eter114 / 115.0 / 3 miles off Clarence River, NSW (S 29˚ 25’ 50”, E 153˚ 25’ 25”) / July 2011 / “Commercial Prawn Trawler” / KM116361, KM116287
AMS I.45555-001 / Eter115 / 124.0 / 3 miles off Clarence River, NSW (S 29˚ 25’ 50”, E 153˚ 25’ 25”) / July 2011 / “Commercial Prawn Trawler” / KM116362,KM116288
AMS I.45555-001 / Eter116 / 116.5 / 3 miles off Clarence River, NSW (S 29˚ 25’ 50”, E 153˚ 25’ 25”) / July 2011 / “Commercial Prawn Trawler” / KM116363,KM116289
AMS I.45555-001 / Eter117 / 120.0 / 3 miles off Clarence River, NSW (S 29˚ 25’ 50”, E 153˚ 25’ 25”) / July 2011 / “Commercial Prawn Trawler” / KM116364,KM116290
AMS I.45555-001 / Eter118 / 130.0 / 3 miles off Clarence River, NSW (S 29˚ 25’ 50”, E 153˚ 25’ 25”) / July 2011 / “Commercial Prawn Trawler” / KM116365, KM116291
AMS I.45555-001 / Eter119 / 117.0 / 3 miles off Clarence River, NSW (S 29˚ 25’ 50”, E 153˚ 25’ 25”) / July 2011 / “Commercial Prawn Trawler” / KM116366, KM116292
AMS I.45555-001 / Eter120 / 119.0 / 3 miles off Clarence River, NSW (S 29˚ 25’ 50”, E 153˚ 25’ 25”) / July 2011 / “Commercial Prawn Trawler” / KM116367,KM116293
AMS I.45555-001 / Eter121 / 123.0 / 3 miles off Clarence River, NSW (S 29˚ 25’ 50”, E 153˚ 25’ 25”) / July 2011 / “Commercial Prawn Trawler” / KM116368, KM116294
AMS I.45555-001 / Eter122 / 119.0 / 3 miles off Clarence River, NSW (S 29˚ 25’ 50”, E 153˚ 25’ 25”) / July 2011 / “Commercial Prawn Trawler” / KM116369, KM116295
AMS I.45555-001 / Eter123 / 127.5 / 3 miles off Clarence River, NSW (S 29˚ 25’ 50”, E 153˚ 25’ 25”) / July 2011 / “Commercial Prawn Trawler” / KM116370, KM116296
AMS I.45555-001 / Eter124 / 117.0 / 3 miles off Clarence River, NSW (S 29˚ 25’ 50”, E 153˚ 25’ 25”) / July 2011 / “Commercial Prawn Trawler” / KM116371, KM116297
AMS I.45555-001 / n/a / 129.5 / 3 miles off Clarence River, NSW (S 29˚ 25’ 50”, E 153˚ 25’ 25”) / July 2011 / “Commercial Prawn Trawler” / n/a
AMS I.45555-001 / n/a / 124.0 / 3 miles off Clarence River, NSW (S 29˚ 25’ 50”, E 153˚ 25’ 25”) / July 2011 / “Commercial Prawn Trawler” / n/a
AMS I.45555-001 / n/a / 120.0 / 3 miles off Clarence River, NSW (S 29˚ 25’ 50”, E 153˚ 25’ 25”) / July 2011 / “Commercial Prawn Trawler” / n/a
SAM F12433 / Eter 99 / 151.0 / Buffalo Reef, Spencer Gulf, South Australia (S 34˚ 43’ 1”, E 136˚ 27’ 31”) / 2011 / A. Ivey, SARDI / KM116385, KM116311
SAM F12434 / Eter 100 / 140.0 / Buffalo Reef, Spencer Gulf, South Australia (S 34˚ 43’ 1”, E 136˚ 27’ 31”) / 2011 / A. Ivey, SARDI / KM116372, KM116298
SAM F12435 / Eter 101 / 148.5 / Thistle Island, Spencer Gulf, South Australia (S 35˚ 1’, E 136˚ 9’) / 2011 / A. Ivey, SARDI / KM116373, KM116299
SAM F12436 / Eter 102 / 122.0 / Wardang Island, Spencer Gulf, South Australia (S 34˚ 30’, E 137˚ 22’) / 2011 / A. Ivey, SARDI / KM116374, KM116300
SAM F12437 / Eter 103 / 147.0 / Thistle Island, Spencer Gulf, South Australia (S 35˚ 1’, E 136˚ 9’) / 2011 / A. Ivey, SARDI / KM116375, KM116301
SAM F12438 / Eter 104 / 144.5 / Thistle Island, Spencer Gulf, South Australia (S 35˚ 1’, E 136˚ 9’) / 2011 / A. Ivey, SARDI / KM116376, KM116302
SAM F12439 / Eter 105 / 154.5 / Thistle Island, Spencer Gulf, South Australia (S 35˚ 1’, E 136˚ 9’) / 2011 / A. Ivey, SARDI / KM116377, KM116303
SAM F12440 / Eter 106 / 149.5 / Thistle Island, Spencer Gulf, South Australia (S 35˚ 1’, E 136˚ 9’) / 2011 / A. Ivey, SARDI / KM116378, KM116304
SAM F12441 / Eter 107 / 148.0 / Thistle Island, Spencer Gulf, South Australia (S 35˚ 1’, E 136˚ 9’) / 2011 / A. Ivey, SARDI / KM116379, KM116305
SAM F12442 / Eter 108 / 148.5 / Thistle Island, Spencer Gulf, South Australia (S 35˚ 1’, E 136˚ 9’) / 2011 / A. Ivey, SARDI / KM116380, KM116306
SAM F12443 / Eter 109 / 142.0 / Thistle Island, Spencer Gulf, South Australia (S 35˚ 1’, E 136˚ 9’) / 2011 / A. Ivey, SARDI / KM116381, KM116307
SAM F12444 / Eter 110 / 146.0 / Thistle Island, Spencer Gulf, South Australia (S 35˚ 1’, E 136˚ 9’) / 2011 / A. Ivey, SARDI / KM116382, KM116308
SAM F12445 / Eter 111 / 148.0 / Thistle Island, Spencer Gulf, South Australia (S 35˚ 1’, E 136˚ 9’) / 2011 / A. Ivey, SARDI / KM116383, KM116309
SAM F12446 / Eter 112 / 146.0 / Thistle Island, Spencer Gulf, South Australia (S 35˚ 1’, E 136˚ 9’) / 2011 / A. Ivey, SARDI / KM116384, KM116310
BPBM 41163 / Eter125 / 216.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116386, KM116312
BPBM 41163 / Eter126 / 195.5 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116387, KM116313
BPBM 41163 / Eter127 / 209 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / n/a
BPBM 41163 / Eter128 / 194.5 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116388, KM116314
BPBM 41163 / Eter129 / 190.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116389, KM116315
BPBM 41163 / Eter130 / 206.5 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116390, KM116316
BPBM 41163 / Eter131 / 195.5 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116391, KM116317
BPBM 41163 / Eter132 / 190.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116392, KM116318
BPBM 41163 / Eter133 / 189.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116393, KM116319
BPBM 41163 / Eter134 / 198.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / n/a
BPBM 41163 / Eter135 / 194.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116394, KM116320
BPBM 41163 / Eter136 / 196.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / n/a
BPBM 41163 / Eter137 / 206.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116395, KM116321
BPBM 41163 / Eter138 / 204.5 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116396, KM116322
BPBM 41163 / Eter139 / 179.5 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116397, KM116323
BPBM 41163 / Eter140 / 210.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116398, KM116324
BPBM 41163 / Eter141 / 211.5 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116399, KM116325
BPBM 41163 / Eter142 / 203.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116400, KM116326
BPBM 41163 / Eter143 / 187.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / n/a
BPBM 41163 / Eter144 / 218.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116401, KM116327
BPBM 41163 / Eter145 / 208.5 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116402, KM116328
BPBM 41163 / Eter146 / 179.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116403, KM116329
BPBM 41163 / Eter147 / 192.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116404, KM116330
BPBM 41163 / Eter148 / 205.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116405, KM116331
BPBM 41163 / Eter149 / 187.5 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116406, KM116332
BPBM 41163 / Eter150 / 216.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116407, KM116333
BPBM 41163 / Eter151 / 202.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116408, KM116334
BPBM 41163 / Eter152 / 186.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116409, KM116335
BPBM 41163 / Eter153 / 205.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116410, KM116336
BPBM 41163 / Eter154 / 188.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116411, KM116337
BPBM 41163 / Eter155 / 205.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116412, KM116338
BPBM 41163 / Eter156 / 221.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116413, KM116339
BPBM 41163 / Eter157 / 208.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / n/a
BPBM 41163 / Eter158 / 197.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / n/a
BPBM 41163 / Eter159 / 208.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116414, KM116340
BPBM 41163 / Eter160 / 183.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116415, KM116341
BPBM 41163 / Eter161 / 215.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116416, KM116342
BPBM 41163 / Eter162 / 198.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116417, KM116343
BPBM 41163 / Eter163 / 180.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116418, KM116344
BPBM 41163 / Eter164 / 170.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116419, KM116345
BPBM 41163 / Eter165 / 158.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116420, KM116346
BPBM 41163 / Eter166 / 153.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116421, KM116347
BPBM 41163 / Eter167 / 141.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116422, KM116348
BPBM 41163 / Eter168 / 158.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116423, KM116349
BPBM 41163 / Eter169 / 142.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116424, KM116350
BPBM 41163 / Eter170 / 163.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116425, KM116351
BPBM 41163 / Eter171 / 156.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116426, KM116352
BPBM 41163 / Eter172 / 165.5 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116427, KM116353
BPBM 41163 / Eter173 / 155.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116428, KM116354
BPBM 41163 / Eter174 / 179.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116429, KM116355
BPBM 41163 / Eter175 / 166.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116430, KM116356
BPBM 41163 / Eter176 / 170.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / n/a
BPBM 41163 / Eter177 / 159.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116431, KM116357
BPBM 41163 / Eter178 / 172.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / n/a
BPBM 41163 / Eter179 / 162.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116432, KM116358
BPBM 41163 / Eter180 / 173.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / n/a
BPBM 41163 / Eter181 / 167.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / KM116433, KM116359
BPBM 41163 / Eter182 / 170.0 / King George Sound, Western Australia (S 35˚ 02’, E 117˚ 56’) / August 25 2011 / T. Leary, Department of Fisheries Western Australia / n/a

1