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This form is designed to collect critical information for engineering your cell line. To provide you the highest quality product and accurate pricing, we ask that you fill this form out completely. Thank you!
Date (mm/dd/yy):
Customer Information
Name
Company/Organization
Address 1
Address 2
City
State
Zip/Postal Code
Country
Phone / Email / Fax
Cell Engineering Questionnaire and Order Information
Project Type / Choose OneKnockoutKnockinOther
Project Description
  • Please give a complete description of the project

Cell Line / Species
Cell Source / Cell Source ID
Gene Name / Gene Symbol
Enter target sequence below Help
  • Identify one single base where you would like cutting to be targeted with brackets, like this …NNNN[N]NNNN…
  • Make sure that there are at least 50 bases on each side of the bracketed sequence. You needn’t give us more sequence than this, but if you feel a need to give us more, the maximum should be no more than 150 bases on either side.
Only DNA sequence goes in this field. No sequence annotations! Put any comments in the Notes field below.
Notes (any other information)
Additional Gene Information
Any reason to believe that changes to this gene will be lethal or result in a growth disadvantage? / Choose OneYesNo
Can you provide a verified genomic sequence of the target gene? / Choose OneYesNo
Gene Editing Process
Is this a random integration or specific gene edit? / Choose OneRandomSpecific
Homozygous or heterozygous edit? / Choose OneHomozygousHeterozygous
If a knock-in, please provide the size of the insert / Choose One<500bp500-1000bp1-2 KB>2 KB
Can the transgene or other components be provided by you or through a commercial source? / Choose OneYesNo
If utilizing our selectable markers, do you want Footprint-Free™ removal? / Choose OneYesNo
Cell Line Information
Do you or provider have mycoplasma certificate for your cell line? / Choose OneYesNo
What is the transfection efficiency of your cell line? / Choose OneUnknown80-100%60-80%40-60%20-40%0-20%
What is the doubling time of your cell line? / Choose One<12 hours24 hours36 hours48 hoursMore than 48 hours
Does a standard protocol for cell growth and transfection exist? / Choose OneYesNo
Is the cell line and/or media proprietary, if so, can you provide maintenance protocol? / Choose OneYesNoYes - we can provide protocol
Please note any special growth characteristics or requirements
Do you know the ploidy of gene in chosen cell line? / Choose OneYesNoMaybe
Is the cell amenable to single cell cloning? / Choose OneYesNo
Please provide any alternative cell line options or provide us with information to determine an appropriate cell line for your project

Transposagen Cell Engineering Order Formv2.0