THE STUDY OF PHENOMEN OF FASCIACIA ON THE ARABIDOPSIS THALIANA.

Nikolaeva Yu.E.

Institute Physiology of Plants and Genetics. Academy of Sciences of Tajikistan. Dushanbe. Tajikistan.

Fasciation is a term used to describe a variety of developmental abnormalities in the shoot system [1]. These include distortions in phyllotaxy and broadening, flattening and in extreme cases, bifurcation of the stem. Fasciation represents a breakdown in the pattern of organogenesis and has been associated with meristematic enlargement. [2] The study of fasciacia might reveal how meristem structure and function are established and maintained in normal plants. The study phenomenon of fasciacia also supports elaboration of methods of rising crop.

Materials and method.

Seeds mutants 90 (clavata pod) and yellow-green mutants 12.6.15, V-76, 58/15 locus Flavi1, mutant 568/5 locus chrolina-5 and mutant 130 locus clavovilidis was used in experiment. [3] The seeds were placed at 4oC for one month and then seeds were sown in a soil. Soil: 60% sand, 40% turf. All plants were grown under 600 ft-candles of constant cool white fluorescent light at 15o-20o C. Seeds were sown at a maximum density of one seed per five cm2.

Results.

We crossed mutant 90 with green-yellow mutants. The plants in the F1 have been wild type. We analyzed F2 generation. (Tab.1) The phenomenon fasciacia of stem was observed among plants class green with clavata pods, owing to the fact that we supposed fasciated plants were heterozygotes.(Photo) The plants F2 generation was grown up and their progeny (the F3 generation) was harvested from each F2 plant separately to give a collection of F3 families. We have (plants F3) lines 14 with different expressivity and penetrance. (Tab2) The plants with phenomenon fasciacia of stem have been positively correlated between fasciacia of stem and the quantity pods and quantity of seeds in the pods. (Tab3)

Conclusion.

The fasciated plants are heterozygots. The fasciated plants have been positively correlated between fasciacia of stem and the quantity pods and quantity of seeds in the pods. The fasciated plants have more pods and quantity of seeds in the pods than mutant 90.

Tab. 1

The F2 segregation in crosses between mutant 90 and yellow-green plants.

Cross / Total / Wild type / Green, cla / Yellow,normal / Yellow, cla / Х2 / Р
9:3:3:1
90х12.6.15 / 156 / 105 / 31
(23) / 15 / 5 / 12,74 / >0,05
90хV-76 / 70 / 49 / 14
(6) / 11 / 0 / 7,33 / <0,05
90х568/5 / 116 / 91 / 19
(10) / 1 / 5 / 43,33 / >0,05
90х58/15 / 130 / 93 / 30
(12) / 4 / 3 / 27,65 / >0,05
90х130 / 126 / 80 / 27
(3) / 12 / 7 / 7,21 / <0,05

Note: in blacket ( ) number of fasciated plants

Tab. 2

Analyse penetrance in F3.

Progeny in crosses between
90х12.6.15 / 90хV-76 / 90х58/15 / 90х130 / 90х568/5
А1 75,47% / B1 52,75% / C1 76,74% / D1 12,28% / E1 24,71%
А2 42% / B2 55,26% / C2 66,67% / D2 0% / E2 96,43%
А3 61,82% / B3 24,39% / C3100% / E3 50%
А4 0% / C4 66,67%

Tab3

Calculation of quantity characteristics in F3.

Mutants / Height
sм. / Quantity
Pod / Quantity seeds in pod / Width of stem
mm. /
Mutant 90 / 30,5+2,2 / 90,6+9,4 / 49,5+3,2 / 1,2+0,02 /
А1 / 23,5+1,6 / 156,5+10 / 75,8+4 / 3,4+0,1 /
А2 / 26,8+1,2 / 148,7+9,5 / 69,7+3,2 / 3,5+0,2 /
А3 / 25,7+1,1 / 147,2+8,2 / 70,6+3,1 / 3,6+0,2 /
B1 / 21,1+0,8 / 160,2+11 / 72,3+4,1 / 4,2+0,4 /
B2 / 20,3+0,7 / 155,6+9,3 / 68,7+3,1 / 4,4+0,2 /
C1 / 15,6+0,3 / 151,4+9,7 / 65,2+2,8 / 4,1+0,1 /
C2 / 22,2+1,4 / 148,3+8,4 / 63,7+2,5 / 3,9+0,3 /
C3 / 21,4+1,7 / 146,2+7,6 / 69,7+3,2 / 3,7+0,2 /
D1 / 23,1+1,3 / 142,3+7,4 / 50,2+3,1 / 1,4+0,06 /
E1 / 25,2+1,5 / 140,4+7,5 / 49,5+2,8 / 3,2+0,05 /
E2 / 21,5+1,4 / 142,3+8,2 / 55,7+3,3 / 3,4+0,2 /
E3 / 22,7+1 / 144,6+8,6 / 57,4+3,4 / 3,5+0,3 /

References

1.Worsdell W.C. (1905) Fasciation: its meaning and origin. New Phytol 55-74.

2. Krichahn D. and Napp-Zinn K. (1975) Fasciation studies in Arabidopsis thaliana (L) Heynh. Arabid.Inf. Serv. 12. 9

3. Usmanov P.J. (1984) Changeable of gens in high plants. Dissertacion