Supplemental Table 1. Strains representing the global distribution of dengue viruses used in the assay development
Serotype determination3'NC / C-prM / NS5
Strain / Origin / Year / Source / IFA / SYBR Green / Multiplex
33532 / Australia / 1990 / Human / 1 / + / 1 / 1
1465 / Belize / 1984 / Mosquito / 1 / + / 1 / 1
BC253/95 / Brazil / 1995 / Human / 1 / + / 1 / 1
S-40568 / Burma / 1976 / Human / 1 / + / 1 / 1
CI00374 / Colombia / 1993 / Human / 1 / + / 1 / 1
BC89/94 / Costa Rica / 1994 / Human / 1 / + / 1 / 1
1715* / Dominica / 1986 / Mosquito / 1 / + / 2 / 2
EC02189 / Ecuador / 1993 / Human / 1 / + / - / 1
NAMRU-3 / Egypt / 1985 / Unknown / 1 / + / 1 / 1
BC63/94 / El Salvador / 1994 / Human / 1 / + / 1 / 1
S-32090 / Fiji / 1975 / Human / 1 / + / 1 / 1
GT00175 / Guatemala / 1990 / Human / 1 / + / 1 / 1
200569 / Haiti / 1983 / Human / 1 / + / 1 / 1
HO00327 / Honduras / 1995 / Human / 1 / + / 1 / 1
276RKI / India / 1997 / Human / 1 / + / 1 / 1
1266 / Indonesia / 1978 / Mosquito / 1 / + / 1 / 1
228690 / Jamaica / 1977 / Human / 1 / + / 1 / 1
MC00895 / Micronesia / 1990 / Human / 1 / + / 1 / 1
BC167/97 / Malaysia / 1997 / Human / 1 / + / 1 / 1
BC202/97 / Mexico / 1997 / Human / 1 / + / 3 / 3
S-16289 / Nauru / 1974 / Human / 1 / + / 1 / 1
BC95/94 / Panama / 1994 / Human / 1 / + / 1 / 1
260705 / Peru / 1990 / Human / 1 / + / 1 / 1
12150 / Philippines / 1984 / Human / 1 / + / 1 / 1
BC245/97 / Puerto Rico / 1994 / Human / 1 / + / 1 / 1
BC116/94 / Saudi Arabia / 1994 / Human / 1 / + / 1 / 1
1669 / Sri Lanka / 1985 / Mosquito / 1 / + / 1 / 1
BC-5015 / Taiwan / 1989 / Human / 1 / + / 1 / 1
BC-5013T / Taiwan / 1988 / Human / 1 / + / 1 / 1
498 RKI / Thailand / 1998 / Human / 1 / + / 1 / 1
S-31062 / Tonga / 1975 / Human / 1 / + / 1 / 1
TD00030 / Trinidad / 1992 / Human / 1 / + / 1 / 1
HD10674 / Senegal / 1980 / Human / 2 / + / 2 / 2
22316 / Australia / 1993 / Human / 2 / + / 2 / 2
BC100/98 / Bolivia / 1998 / Human / 2 / + / 2 / 2
BC248/95 / Brazil / 1995 / Human / 2 / + / 2 / 2
S-40921 / Burma / 1976 / Human / 2 / + / 2 / 2
CI00405 / Colombia / 1993 / Human / 2 / + / 2 / 2
CR00172 / Curacao / 1993 / Human / 2 / + / 2 / 2
3956 / Senegal / 1983 / Unknown / 2 / + / 2 / 2
EC02133 / Ecuador / 1993 / Human / 2 / + / 2 / 2
10 / Egypt (Somalia) / 1984 / Human / 2 / + / 2 / 2
373915 / Guatemala / 1979 / Human / 2 / + / 2 / 2
1474 / Haiti / 1984 / Human / 2 / + / 2 / 2
S-17850 / Hawaii / 1974 / Human / 2 / + / 2 / 2
85-428 / Indonesia / 1985 / Human / 2 / + / 2 / 2
HA242-82 / Ivory Coast / 1982 / Human / 2 / + / 2 / 2
MEYERS / Kenya / 1982 / Human / 2 / + / 2 / 2
P8-1407 / Malaysia / 1968 / Monkey / 2 / + / 2 / 2
BC145/97 / Malaysia / 1997 / Human / 2 / + / 2 / 2
BC124/97 / Malaysia / 1997 / Monkey / 2 / + / 2 / 2
IMTSSA703 / Martinique / 1998 / Human / 2 / + / 2 / 2
BC17/97 / Mexico / 1996 / Human / 2 / + / 2 / 2
S-9290 / New Caledonia / 1972 / Human / 2 / + / 2 / 2
BC62/94 / Nicaragua / 1994 / Human / 2 / + / 2 / 2
239127 / Palau / 1988 / Human / 2 / + / 2 / 2
BC171/96 / Philippines / 1996 / Human / 2 / + / 2 / 2
BC141/96 / Puerto Rico / 1994 / Human / 2 / + / 2 / 2
S-5285 / Samoa / 1972 / Human / 2 / + / 2 / 2
BC102/94 / Saudi Arabia / 1994 / Human / 2 / + / 2 / 2
S630/62 / Singapore / 1962 / Human / 2 / + / 2 / 2
BC25/96 / Sri Lanka / 1996 / Human / 2 / + / - / 2
S-19966 / Tahiti / 1975 / Human / 2 / + / 1 / 1
BC-5007 / Taiwan / 1989 / Human / 2 / + / 2 / 2
MK-244-87 / Thailand / 1987 / Human / 2 / + / 2 / 2
D79-069 / Thailand/Burma / 1979 / Human / 2 / + / 2 / 2
S-14635 / Tonga / 1974 / Human / 2 / + / 2 / 2
8211085 / Trinidad / 1982 / Human / 2 / + / 2 / 2
UV0190 / Upper Volta / 1983 / Human / 2 / + / 2 / 2
BC27/96 / Vietnam / 1995 / Human / 2 / + / 2 / 2
BC74/97 / Australia / 1993 / Human / 3 / + / 3 / 3
S-40580 / Burma / 1976 / Human / 3 / + / 3 / 3
II00032 / India / 1993 / Human / 3 / + / 3 / 3
BC14/97 / Malaysia / 1997 / Human / 3 / + / 3 / 3
BC188/97 / Mexico / 1997 / Human / 3 / + / 3 / 3
1558 / Mozambique / 1985 / Mosquito / 3 / + / 3 / 3
317685 / Nauru / 1994 / Human / 3 / + / 3 / 3
BC13/96 / Panama / 1994 / Human / 3 / + / 3 / 3
BC182/96 / Philippines / 1997 / Human / 3 / + / 3 / 3
FI00401 / Cook Islands / 1991 / Human / 3 / + / 3 / 3
329659 / Samoa / 1995 / Human / 3 / + / 3 / 3
271242 / Sri Lanka / 1991 / Human / 3 / + / 3 / 3
MK-594-87 / Thailand / 1987 / Human / 3 / + / 3 / 3
VL-M-422/98 / Vietnam / 1998 / Unknown / 3 / + / - / 3
BC169/95 / Mexico / 1985 / Human / 4 / + / 4 / 4
1385 / Brazil / 1982 / Human / 4 / + / 4 / 4
S-40946 / Burma / 1976 / Human / 4 / + / 4 / 4
299649 / Colombia / 1993 / Human / 4 / + / 4 / 4
CS00123 / Costa Rica / 1994 / Human / 4 / + / 4 / 4
CR00163 / Curacao / 1993 / Human / 4 / + / 4 / 4
5168 / Senegal / 1983 / Human / 4 / + / 4 / 4
BC65/94 / El Salvador / 1994 / Human / 4 / + / 4 / 4
BC71/94 / Honduras / 1994 / Human / 4 / + / 4 / 4
HO00323 / Honduras / 1995 / Human / 4 / + / 4 / 4
1269 / Indonesia / 1978 / Mosquito / 4 / + / 4 / 4
BC123/97 / Malaysia / 1997 / Monkey / 4 / + / 4 / 4
BC13/97 / Malaysia / 1997 / Human / 4 / + / 4 / 4
329155 / Martinique / 1995 / Human / 4 / + / 4 / 4
BC287/97 / Mexico / 1997 / Human / 4 / + / 4 / 4
BC59/94 / Nicaragua / 1994 / Human / 4 / + / 4 / 4
D2399 / Nauru / 1980 / Human / 4 / + / 4 / 4
12123 / Philippines / 1984 / Human / 4 / + / 4 / 4
BC258/97 / Puerto Rico / 1994 / Human / 4 / + / 4 / 4
5168 / Senegal / 1983 / Unknown / 4 / + / 4 / 4
BC19/96 / Sri Lanka / 1994 / Human / 4 / + / 4 / 4
057-005-85 / Tahiti / 1985 / Human / 4 / + / 4 / 4
BC-5006 / Taiwan / 1989 / Human / 4 / + / 4 / 4
D85-019 / Thailand / 1985 / Human / 4 / + / 4 / 4
JQ11-90 / Venezuela / 1990 / Human / 4 / + / 4 / 4
*: The specimens with inconsistent test results were highlighted by bold and italic.
Supplemental Table 2. Serotyping capability using the multiple dengue serotype-infected virus panel.
Serotype determinationSYBR Green (Ct) / NS5-TaqMan (Ct)
Virus / 3’NC / C-prM / Multiplex / Single dye
Strain / Origin / Year / Source / IFA / DENV / D1 / D2 / D3 / D4 / D1 / D2 / D3 / D4 / D1 / D2 / D3 / D4
2-HA / Vietnam / 1995 / Unknown / 1, 2, 3 / 11.3 / 15.0 / 12.2 / 10.8 / 21.5 / 27.5 / 13.7
BC31/96a / Mexico / 1996 / Human / 1, 3, 4 / 8.6 / 16.1 / 14.2 / 15.8 / 17.3 / 40.2 / 15.5 / 15.2
BC179/96 / Philippines / 1996 / Human / 1, 3, 4 / 10.7 / 23.0 / 10.7 / 18.8 / 19.9 / 18.5
RKI-746 / Germany / 1995 / Human / 1, 2 / 7.7 / 10.2 / 18.3 / 20.9 / 15.0 / 42.0
BC88/97 / Indonesia / 1975 / Mosquito / 1, 2 / 10.4 / 10.6 / 24.1 / 20.4 / 13.0
BC166/96 / Philippines / 1996 / Human / 1, 2 / 9.8 / 10.0 / 26.1 / 18.3 / 12.0
BC169/96 / Philippines / 1996 / Human / 1, 2 / 9.5 / 16.7 / 10.6 / 21.2 / 9.0
1835 / Puerto Rico / 1987 / Mosquito / 1, 2 / 8.4 / 22.2 / 16.1 / 30.6 / 17.0
S-M-384/95 / Vietnam / 1995 / Human / 1, 2 / 9.9 / 14.7 / 12.0 / 19.3 / 12.7
BC71/97 / Australia / 1993 / Human / 1, 3 / 8.5 / 18.2 / 7.3 / 14.8 / 41.0 / 13.8
BC73/97 / Australia / 1993 / Human / 1, 3 / 11.8 / 19.1 / 9.2 / 17.0 / 40.7 / 18.8
BC116/96 / Indonesia / 1976 / Human / 1, 3 / 11.4 / 25.6 / 14.1 / 22.4 / 17.3
BC72/97 / Australia / 1993 / Human / 1, 3 / 9.2 / 19.7 / 7.9 / 14.4 / 14.9
BC90/97 / Indonesia / 1976 / Mosquito / 2, 3 / 8.1 / 16.6 / 18.5 / 18.5 / 13.1
BC15/97 / Malaysia / 1997 / Mosquito / 2, 3 / 7.8 / ND / ND / ND / ND / 16.3 / 13.6 / 15.4 / 14.4
BC51/97 / Indonesia / 1976 / Mosquito / 2, 4 / 9.4 / 14.0 / 9.3 / 15.7 / 27.2 / 15.9 / 18.7
BC117/96 / Indonesia / 1976 / Human / 3, 4 / 8.0 / 13.6 / 20.1 / 17.9 / 15.4
S-1037 / Indonesia / 1976 / Human / 3, 4 / 7.4 / 12.6 / 20.1 / 17.9 / 13.9
BC113/96 / Indonesia / 1976 / Human / 3, 4 / 11.9 / 12.9 / 14.4 / 21.7 / 29.5 / 20.5 / 26.2
BC167/96 / Philippines / 1996 / Human / 3, 4 / 8.1 / 8.0 / 17.2 / 14.7 / 14.5
BC177/96 / Philippines / 1996 / Human / 3, 4 / 7.9 / ND / ND / ND / ND / 15.5 / 14.1
BC165/96 / Philippines / 1996 / Human / 3, 4 / 10.8 / 8.8 / 18.0 / 15.4 / 13.4
BC164/96 / Philippines / 1996 / Human / 3, 4 / 8.0 / ND / ND / ND / ND / 16.2 / 15.4
BC176/96 / Philippines / 1996 / Human / 3, 4 / 10.5 / 14.3 / 19.8 / 15.7 / 12.6 / 42.2
BC168/96 / Philippines / 1996 / Human / 3, 4 / 11.1 / ND / ND / ND / ND / 13.9 / 13.1
BC181/96 / Philippines / 1997 / Human / 3, 4 / 9.4 / ND / ND / ND / ND / 17.5 / 16.4
BC180/96 / Philippines / 1997 / Human / 3, 4 / 10.2 / 10.6 / 16.2 / 16.6 / 14.3
BC183/96 / Philippines / 1997 / Human / 3, 4 / 7.9 / ND / ND / ND / ND / 15.4 / 15.0
BC170/96 / Philippines / 1996 / Human / 3, 4 / 10.7 / 16.4 / 16.9 / 16.4 / 13.3
BC21/96 / Sri Lanka / 1995 / Human / 3, 4 / 10.6 / ND / ND / ND / ND / 13.0 / 12.4
BC23/96 / Sri Lanka / 1995 / Human / 3, 4 / 10.9 / 7.5 / 16.9 / 14.1 / 12.4
1-HA / Vietnam / 1995 / Human / 3, 4 / 7.7 / 8.9 / 16.0 / 14.2 / 13.1
BC115/96 / Indonesia / 1976 / Human / 1, 3, 4 / 11.2 / ND / ND / ND / ND / 17.9 / 16.7
BC118/96 / Indonesia / 1976 / Human / 1, 3, 4 / 8.3 / 19.2 / 10.8 / 23.1 / 15.9 / 14.7
a: The specimens containing multiple DENV serotypes that were confirmed by at least one RT-PCR assay were highlighted by bold.
ND: not done.