Supplementary Table 2

The partial results of germination test of T1 transgenic plants seeds on 1/2 MS medium with 50mg/l hygromycin

Transgenic lines / No. of inoculated seed (n) / No. of germinated seeds (A) / No. of did not germinate seeds (a) / value for 3:1 / Test
Nc / 30 / 0 / 30 / －
OC1-1 / 55 / 55 / 0 / ＋
OC1-2 / 51 / 51 / 0 / ＋
OC1-3 / 45 / 45 / 0 / ＋
OC1-4 / 60 / 45 / 15 / 0.02 / *
OC1-5 / 58 / 46 / 12 / 0.37 / *
OC1-6 / 44 / 30 / 14 / 0.76 / *
OC1-7 / 51 / 34 / 17 / 1.47 / *
OC1-8 / 64 / 52 / 12 / 1.02 / *
OC2-1 / 57 / 46 / 11 / 0.7 / *
OC2-2 / 53 / 43 / 10 / 0.76 / *
OC2-3 / 30 / 0 / 30 / －
OC2-4 / 42 / 0 / 42 / －
OC2-5 / 30 / 0 / 30 / －
OC2-6 / 30 / 0 / 30 / －
OC2-7 / 30 / 0 / 30 / －
RC1-1 / 44 / 44 / 0 / ＋
RC1-2 / 39 / 39 / 0 / ＋
RC1-3 / 42 / 42 / 0 / ＋
RC1-4 / 40 / 27 / 13 / 0.83 / *
RC1-5 / 42 / 32 / 10 / 0.00 / *
RC1-6 / 50 / 32 / 16 / 1.3 / *
RC1-7 / 34 / 29 / 5 / 1.41 / *
RC2-1 / 45 / 34 / 11 / 0.007 / *
RC2-2 / 40 / 35 / 5 / 2.7 / *
RC2-3 / 32 / 25 / 7 / 0.04 / *
RC2-4 / 30 / 0 / 30 / －
RC2-5 / 30 / 0 / 30 / －
RC2-6 / 30 / 0 / 30 / －
RC2-7 / 30 / 0 / 30 / －

＋：indicate the positive homozygous seeds; －：indicate the negative seeds; *：indicate the heterozygous seeds

The Chi-square goodness of fit test:

= ， =1， =3.84

The above equation is the simplified form of the equation which is Χ2 = Σ [ (Oi - Ei)2 / Ei ].

In this example, if our value of is less than =3.84, we conclude that the sample data come from a population having a 3:1 ratio of A to a. This is consistent with a Mendelian explanation for the data. The genome of these seeds contains single copy T-DNA insertion.