The following reagents are available, free of charge, to users of the Center for Molecular Studies in Digestive and Liver Disease / Morphology Core
I) Primary Antibodies
rabbit anti-actin (Sigma A-2066)
mouse anti (smooth muscle) Actin (NeoMarkers)
Guinea pig anti-adipophilin (RDI)
rabbit anti Apoptosis Inducing Factor (Chemicon)
rabbit anti-(phospho)-AKT (serine 473) (Cell Signaling Tech)
rabbit anti-Amphiregulin (Neomarkers)
goat anti human Amylase (Santa Cruz)
rabbit anti-amylin (rat) (Phoenix Pharmaceuticals)
rabbit anti-Asialo GM1 (natural killer cell marker) (Wako)
mouse anti-ATPase proton pump (MBL)
sheep anti BrdU (US Biologicals)
mouse anti beta catenin (BD Transduction
goat anti-betagalactosidase (ICN Cappel Inc)
rabbit anti beta-Galactosidase (Molecular Probes
rabbit anti beta-Galactosidase (AbCam
rabbit anti-human/mouse activated Caspase 3 (R&D Systems)
mouse anti-human CCR6 (R&D Systems)
mouse monoclonal anti human cyclin D1 (Pharmingen)
rabbit anti-CBP (Santa Cruz
rat anti-CD3 (Novocastra)
mouse anti-CD4 (NeoMarkers/LavVision)
rat anti-mouse CD4 (American Research Products)
rat anti-mouse CD8 (American Research Products)
mouse anti human CD29 (Pharmingin)
rat anti-mouse CD34 (Abcam)
mouse anti-human CD34 (BD Biosci.)
rat anti-CD45R/B220 (Pharmingen)
rat anti-human CD49f (Chemicon)
rat anti-mouse CD90.2 (Thy-1.2) (PharMingen)
rat anti-mouse CD11b (Integrin alphaM chain) (PharMingen)
mouse monoclonal anti human CD68 (Novacastra)
rat anti-CD138 (RDI)
goat anti- clustrin & blocking peptide (Santa Cruz)
rabbit anti-rat collagen type I (Accurate Chemicals)
rabbit anti-mouse collagen type IV (Chemicon)
rabbit anti- chromogranin A (Diasorin)
rabbit anti-Claudin 7 (Zymed Laboratories)
rabbit anti-Cox-2(murine) (Cayman Chemicals)
goat anti Cox-2(murine) (Santa Cruz)
mouse anti- cytochrome C (Pharmingin)
mouse anti-human cytokeratin 4 (NovaCasta)
mouse anti-human cytokeratin 5 (NovaCasta)
mouse anti-human cytokeratin 13 (NovaCasta)
rat anti- cytokeratin 19 (TROMA III) (Dev. Stud. Hybridoma Bank)
rabbit anti-human desmocollin 2 (RDI)mouse anti-desmin (Sigma)
mouse anti-desmin (Sigma)
mouse anti-E-Cadherin (Transduction Laboratories)
mouse monoclonal anti human EGF receptor (Biodesign)
rabbit anti multi-species EGF receptor (RDI)
rabbit anti EGF receptor (Calbiochem)
goat anti EGF receptor (Santa Cruz)
rabbit anti-EP2 (Cayman Chemicals)
mouse anti-FAS / CD95 / APO-1 (Transduction Laboratories)
mouse anti human liver fatty acid binding protein (Novo Castra)
rabbit anti pFKHR(Thr24)/FKHR L1(Thr32)
aka Fox01a / Fox03a (Cell Signaling)
goat anti-Fibronectin receptor (integrin a5b1) (Chemicon)
rabbit anti-mouse fibronectin (Chemicon)
rat anti-mouse F4/80 (Serotec)
mouse anti- human Filaggrin (NovoCastra)
mouse-anti mouse Flk-1 (Santa-Cruz)
rabbit anti-GASP (Nova Castra)
rabbit anti-human gastrin (Cappel)
goat anti-GATA 4 (Santa Cruz)
rabbit anti-GFP epitope tag (ABR)
goat anti-GKLF (Santa Cruz)
guinea pig anti glucagon (Linco)
rabbit anti- glucagon (Biodesign)
goat anti-glucagon (Santa Cruz)
mouse anti-HIF 1 alpha (Novus)
goat anti-human HNF-1 alpha (Santa Cruz)
goat anti-mouse HNF-3 beta (Santa Cruz)
goat anti-human HNF-4 alpha (Santa Cruz)
rabbit anti-phospho-Histone H3 Mitosis Marker (Upstate Biotech.)
mouse anti-human IGFBP-3 (R&D Systems)
rabbit anti-mouse IGFBP-3 (GroPrep)
rabbit anti-mouse IGFBP-5 (GroPep)
rat monoclonal anti-human IL-6 (Pharmingin)
goat anti-(rat) IL-6 (R-19) (Santa Cruz)
mouse anti-rat IRS-1 (Chemicon)
rabbit anti-mouse phosho-IRS-1 (Affinity Bioreagents)
guinea pig anti-insulin (Dako)
goat anti-human (precursor form) integrin al.2 (N-19) (Santa Cruz)
rabbit anti-human integrin alpha 3 (Chemicon)
rabbit anti-integrin beta-4 subunit (Chemicon)
rabbit anti-integrin alpha 5 subunit (Chemicon)
rabbit anti-Ki67 (Nova Castra Laboratories)
mouse anti-LFABP (Nova Castra Laboratories)
mouse anti-Luciferase (Sigma)
rat anti-mouse metallophilic macrophages (Serotec)
goat anti-mouse MIP 3 alpha (R&D Systems)
goat anti-human MIP 3 alpha (R&D Systems)
mouse anti human MUC-2 (NovoCastra)
rabbit anti mouse MUC-2 (Biomeda)
rabbit anti-human N-cadherin (R&D)
rabbit anti-Notch-1 (Santa Cruz)
mouse anti-syrian hamster Notch-1 (Calbiochem)
goat anti NPY (C-20) (Santa Cruz)
goat anti NPY2-receptor (Santa Cruz)
mouse anti- Macrophage (NovoCastra)
anti-minichromosome maintainence protein 2 (Vector)
mouse anti- Osteopontin (R&D)
rabbit anti pancreatic polypeptide (Linco)
mouse anti- pan cytokeratin (Sigma)
mouse monoclonal anti PCNA (DAKO)
rabbit anti-mouse Perelipin (Affinity Bioreagents)
rabbit anti-mouse p16 (Santa Cruz)
mouse anti-human p21 (Pharmingen)
rabbit anti-p27 (KIP1) (Upstate)
mouse anti-p53 (NovoCastra)
mouse anti-p53 gene suppressor product (Biogenex)
goat anti-PML (Santa Cruz)
mouse anti-PML (Santa Cruz)
goat anti- TA p63 (Santa Cruz)
goat anti- ∆N p63 (Santa Cruz)
goat anti- p63 (Santa Cruz)
goat anti-PPAR alpha (C-20) (Santa Cruz)
rabbit anti phospho S6 ribosomal prot. (Cell Signaling)
rabbit anti- S-100 (Novocastra)
mouse anti human small intestine mucinous antigen (NovoCastra)
goat anti-Somatostatin (Santa Cruz)
rabbit anti-somatostatin (Peninsula Labs, Inc.)
STAT3 (Cell Signaling)
phospho STAT3 (Cell Signaling)
mouse anti-SUMO-1 (Santa Cruz)
goat anti- TGase-1 (Santa Cruz)
mouse anti-TGF alpha (Oncogene)
hamster anti (mouse, rat, rabbit) TNF-alpha (genzyme R&D systems)
goat anti-TRH R1 (Santa Cruz)
goat anti-TRH R1 (Santa Cruz)
mouse anti-T7 Tag (Novagen)
rabbit anti-mouse Uncoupling protein 11-A (Alpha Diagnostic)
rabbit anti-Von Willebrand Factor (Dako)
goat villin & blocking peptide (Santa Cruz)
mouse (IgM) anti- vimentin (Sigma)
rabbit anti- WAF 1 (Calbiochem)
mouse anti-YY1 (H10) (Santa Cruz)
II) Biotinylated Antibodies
goat anti-chicken (Vector)
rabbit anti-goat (Vector)
goat anti-guinea pig (Vector)
goat anti-hamster (Vector)
goat anti-human (Vector)
goat anti-mouse (Vector)
horse anti-mouse (Vector-pre-adsorbed on rat)
goat anti-rabbit (Vector)
rabbit anti-rat (Vector-pre-adsorbed on mouse specifically for use on mouse tissue)
anti-sheep (Vector)
goat anti-syrian hamster (EBioscience) only works for Syrian Hamster primaries
anti-mouse IgM
III) Conjugated Antibodies
Alexa Fluor 594 goat anti-mouse (confocal only)
Alexa Fluor 594 goat anti-rabbit (confocal only)
Alexa Fluor 488 goat anti-mouse
Alexa Fluor 488 goat anti-rabbit
AMCA donkey anti-goat (Jackson Immuno Research)
AMCA donkey anti-rabbit (Jackson Immuno Research)
alkaline phosphotase sheep anti-digoxigenin (Boehringer)
Cy 2 donkey anti chicken (Jackson Immuno Research)
Cy 2 donkey anti guinea pig (Jackson Immuno Research)
Cy 2 goat anti mouse (Jackson Immuno Research)
Cy 2 donkey anti rabbit (Jackson Immuno Research)
Cy 2 donkey anti mouse (Jackson Immuno Research)
Cy 2 donkey anti goat (Jackson Immuno Research)
Cy 2 donkey anti rat (Jackson Immuno Research)
Cy 2 donkey anti sheep (Jackson Immuno Research)
Cy2 goat anti mouse IgG2a (Jackson Immuno Research)
Cy2 goat anti mouse IgG3 (Jackson Immuno Research)
Cy2 Strepavidin (Jackson Immuno Research)
Cy 3 Strepavidin (Jackson Immuno Research)
Cy 3 donkey anti chicken (Jackson Immuno Research)
Cy 3 donkey anti rabbit (Jackson Immuno Research)
Cy 3 donkey anti goat (Jackson Immuno Research)
Cy 3 donkey anti mouse (Jackson Immuno Research)
Cy 3 donkey anti sheep (Jackson Immuno Research)
Cy 3 donkey anti guinea pig (Jackson Immuno Research)
Cy 3 donkey anti rat (Jackson Immuno Research)
Cy 3 mouse anti anti-digoxigenin (Jackson Immuno Research)
Cy 3 mouse anti-FLAG (Sigma)
Cy5 donkey anti chicken (Jackson Immuno Research)
Cy5 donkey anti guinea pig (Jackson Immuno Research)
Cy5 donkey anti rabbit (Jackson Immuno Research)
Cy5 donkey anti mouse (Jackson Immuno Research)
Cy5 donkey anti goat (Jackson Immuno Research)
Cy5 donkey anti rat (Jackson Immuno Research)
Cy5 donkey anti sheep (Jackson Immuno Research)
FITC goat anti-rabbit (BMB)
FITC goat anti-rat (BMB)
FITC mouse anti- WAF1
HRP goat anti rabbit (purified and adsorbed with human IgG) (Sigma Immuno)
HRP rabbit anti chicken (Sigma Immuno)
HRP rat anti mouse IgM (Zymed)
rhodamine goat anti-mouse (Cappel)
rhodamine goat anti-rabbit (Chemicon (former BMB))
rhodamine goat anti-guinea pig (Cappel)
rhodamine goat anti mouse (BMB)
Bauhinia purpurea (FITC) (Vector)
Anguilla anguilla lectin (AAA) (Texas Red conjugated) (EY Laboratories)
Arachis hypogaea (TRITC conjugated) (Sigma)
Dolichos biflorus (FITC conjugated) (Sigma)
Helix pomatia (FITC conjugated) (Sigma)
IV) Lectins
Alevria aurantia (biotinylated)
Anguilla anguilla lectin (AAA) (Texas Red conjugated) (EY Laboratories)
Anguilla anguilla lectin (AAA) (biotinylated) (EY Laboratories)
Arachis hypogaea (PNA) (TRITC conjugated) (Sigma)
Arachis hypogaea (PNA) (biotinylated) (EY Laboratories)
Bauhinia purpurea (BPA) (FITC conjugated) (Vector)
Dolichos biflorus (DBA) (FITC conjugated) (Sigma)
Dolichos biflorus (DBA) (biotinylated) (EY Laboratories)
Helix pomatia (HPA) (FITC conjugated) (Sigma)
Helix pomatia (HPA) (biotinylated) (EY Laboratories)
Griffonia Bandeiraea simplifolia I isolectin B4 (biotinylated) (Vector)
Jacalin (biotinylated) (Vector)
Lycopersicon esculentum (LEI) (biotinylated) (Vector)
Ulex Europaeus Agglutinin I (UEA I) (biotinylated) (Vector)
V) Kits
VectaStain Elite ABC kit (DAB reporter) (Vector)
VectaStain Elite ABC kit (alkaline phosphotase reporter) (Vector)
Avidin / Biotin blocking kit (Vector)
RNA MAXX Transcription kit (Stratagene)
DIG RNA Labelling Kit SP6/T7 (Roche)
Peroxidase substrate kit DAB (Vector)
Vecta-Red alkaline phosphotase substrate (Vector)
NovaRED peroxidase substrate (Vector)
Metal-enhanced DAB substrate (Pierce)
Histomark X-Gal Kit (Kirkegaard)
Vector VIP HRP Substrate Kit (Vector)
M.O.M. kit (Vector)
VI) Miscellaneous Reagents:
Acridine Orange (Molecular Probes)
Alexafluor 546 phalloidin (Molecular Probes #A22283)
AMCA-Avidin (Vector)
anti-mouse IgG (unconjugated)
goat anti-mouse IgG Fab fragments (unconjugated) (Jackson Immunoscience)
goat anti-rabbit IgG Fab fragments (unconjugated) (Jackson Immunoscience)
BCIP / NBT (alkaline phosphotase substrate) (Boehringer)
CDP blocking peptide
Crystal/Mount
Cytoseal mounting medium
Crystal Violet (Sigma)
DAPI (Molecular Probes)
Evan’s Blue (ICN)
Fast Green (Fisher)
FITC-Avidin
Hoechhst 33342 (Molecular Probes)
HNF1 alpha blocking peptide
HNF 1ß blocking peptide
Mouse IgG (Vector)
Mouse IgG1 (Dako)
Methyl Green (Vector)
Mitotracker Green FM (Molecular Probes)
Neutral Red (Fisher)
Non-immune sera (goat, calf, horse, rabbit, mouse, donkey, guinea pig)
Neuraminidase (Sigma)
Propidium iodide (Molecular Probes)
Proteinase K solution (Boehringer)
Rabbit IgG (Vector)
Safranin O (Sigma)
Sudan Black B (Sigma)
Sudan Black B (BDH)
SuperBlock blocking reagent for PBS (Pierce)
SYTO 13 (fluorescent orange nuclear counterstain for confocal) (Molecular Probes)
TRITC-Avidin (Vector)
Universal protein blocker (Coulter)
Vectashield fluorescent mounting medium (Vector)
Vectashield fluorescent mounting medium with DAPI (Vector)
Vectashield fluorescent mounting medium with Propidium Iodide (Vector)
YoYo (DNA fluor stain) (Molecular Probes)
Last update: 05/10/07