The Early Stages of Tumor Angiogenesis of Human Osteosarcoma: a Nude Mice Xenotransplant Model

Supplementary material

The early stages of tumor angiogenesis of human osteosarcoma: A nude mice xenotransplant model

Francisco Giner1, José Antonio López-Guerrero2, Isidro Machado1,3, Silvia Calabuig-Fariñas1, Zaida García-Casado2, Amando Peydró-Olaya1, Antonio Llombart-Bosch1,*.

1Department of Pathology, Universitat de València Estudi General (UVEG), Valencia, Spain.

2Laboratory of Molecular Biology and 3Department of Pathology, Fundación Instituto Valenciano de Oncología (FIVO), Valencia, Spain.

This study was supported by grants of the 6th FP of the EC: Prognosis and Therapeutic Targets in the Ewing Family of Tumors (PROTHETS), Contract number: 503036; and EuroBoNeT Network, contract number: 018814.

Key words: osteosarcoma, angiogenesis, nude mice xenograft, chemokines.

*Corresponding author:

Antonio Llombart-Bosch, MD

Department of Pathology,

University of Valencia,

Avda. Blasco Ibañez, 17;

46010 Valencia, Spain,

Tel.: +34 96 3864146,

E-mail:

Index pages

Table 1S / 3
Figure 1S / 6
Figure 2S / 7
Figure 3S / 8
Figure 4S / 9
Table 2S / 10
Table 3S / 13

Table 1S. Genes and assays included in the Low Density Array for the study of the expression of angiogenic factors by quantitative RT-PCR.

N / Gene symbol / Assay reference
(Applied Biosystems) / Amplicon size (pb) / OMIM
Specific promoters and inhibitors
1 / AMOT (angiomotin) / Hs00611096_m1 / 65 / 300410
2 / ANG (angiogenina) / Hs00265741_s1 / 91 / 105850
3 / ANGPT1 (angiopoietin-1) / Hs00181613_m1 / 87 / 601667
4 / ANGPT2 (angiopoietin-2) / Hs00169867_m1 / 73 / 601922
5 / CHGA (Vasostatin/Chromogranin-A) / Hs00154441_m1 / 115 / 118910
Growth factors and receptors
6 / Ephrin 2A (EFNA2) / Hs00154858_m1 / 89 / 602756
7 / Ephrin-A5 (EFNA5) / Hs00157342_m1 / 98 / 601535
8 / Ephrin-B2 (EFNB2) / Hs00187950_m1 / 63 / 600527
9 / EPHB4 (Ephrin B4) / Hs00174752_m1 / 82 / 600011
10 / FGF1 (aFGF) / Hs00265254_m1 / 65 / 131220
11 / FGF2 (bFGF) / Hs00266645_m1 / 82 / 134920
12 / FGF4 / Hs00173564_m1 / 53 / 164980
13 / FGF6 / Hs00173934_m1 / 64 / 134921
14 / FGF7 / Hs00384281_m1 / 85 / 148180
15 / FGFR1 / Hs00241111_m1 / 81 / 136350
16 / FGFR2 (KGFR) / Hs00240792_m1 / 77 / 176943
17 / FGFR3 / Hs00179829_m1 / 104 / 134934
18 / FGFR4 / Hs00242558_m1 / 74 / 134935
19 / PDGFA / Hs00234994_m1 / 93 / 173430
20 / PDGFB / Hs00234042_m1 / 80 / 190040
21 / PDGFRA / Hs00183486_m1 / 92 / 173490
22 / PDGFRB / Hs00182163_m1 / 86 / 173410
23 / PF4 (Platelet factor 4) / Hs00236998_m1 / 86 / 173460
24 / TGFA / Hs00177401_m1 / 95 / 190170
25 / TGFB1 / Hs00171257_m1 / 63 / 190180
26 / TGFB2 / Hs00234244_m1 / 92 / 190220
27 / TGFB3 / Hs00234245_m1 / 75 / 190230
28 / TGFBR1 / Hs00610319_m1 / 126 / 190181
29 / TGFBR2 / Hs00234253_m1 / 70 / 190182
30 / TGFBR3 / Hs00234257_m1 / 60 / 600742
31 / FIGF (VEGFD) / Hs00189521_m1 / 130 / 300091
32 / FLK1 (KDR) / Hs00176676_m1 / 84 / 191306
33 / FLT1 (VEGFR2) / Hs00176573_m1 / 55 / 165070
34 / PGF / Hs00182176_m1 / 56 / 601121
35 / VEGF / Hs00173626_m1 / 77 / 192240
36 / VEGFB / Hs00173634_m1 / 69 / 601398
37 / VEGFC / Hs00153458_m1 / 126 / 601528
38 / FLT4 (VEGFR3) / Hs01047679_m1 / 89 / 136352
39 / CD36 / Hs00169627_m1 / 77 / 173510
40 / EDG1 / Hs00173499_m1 / 102 / 601974
41 / EGF / Hs00153181_m1 / 95 / 131530
42 / EGFR / Hs00193306_m1 / 69 / 131550
43 / GRO1 (CXCL1) / Hs00236937_m1 / 70 / 155730
44 / HGF (Scatter factor) / Hs00300159_m1 / 92 / 142409
45 / IGF1 / Hs00153126_m1 / 70 / 147440
46 / IGF1R / Hs00181385_m1 / 77 / 147370
47 / TEK (Tie-2) / Hs00176096_m1 / 82 / 600221
48 / TIE1 / Hs00178500_m1 / 74 / 600222
Cytokines and chemokines
49 / CSF3 / Hs00236884_m1 / 78 / 138970
50 / IFNA1 / Hs00256882_s1 / 115 / 147660
51 / IFNB1 / Hs00277188_s1 / 134 / 147640
52 / IFNG / Hs00174143_m1 / 79 / 147570
53 / IL10 / Hs00174086_m1 / 119 / 124092
54 / IL12A / Hs00168405_m1 / 67 / 161560
55 / IL8 / Hs00174103_m1 / 101 / 146930
56 / MDK / Hs00171064_m1 / 102 / 162096
57 / NRP1 (neuropilin-1) / Hs00826129_m1 / 97 / 602069
58 / PRL (prolactin) / Hs00168730_m1 / 76 / 176760
59 / PTN (pleiotrophin) / Hs00383235_m1 / 76 / 162095
60 / SCYA2 / Hs00234140_m1 / 101 / 158105
61 / SPARC / Hs00277762_m1 / 122 / 182120
62 / TNFA / Hs00174128_m1 / 80 / 191160
63 / TNFSF15 (VEGI) / Hs00353710_s1 / 97 / 604052
Adhesion molecules
64 / CDH5 (VE-Cadherin) / Hs00174344_m1 / 66 / 601120
65 / ITGA5 (integrin a5) / Hs00233743_m1 / 86 / 135620
66 / ITGAV (integrin aV) / Hs00233808_m1 / 64 / 193210
67 / ITGB3 / Hs00173978_m1 / 89 / 173470
68 / PECAM1 (CD31) / Hs00169777_m1 / 65 / 173445
Matrix Proteins, Proteases and Inhibitors
69 / ADAMTS1 (METH1) / Hs00199608_m1 / 68 / 605174
70 / ADAMTS8 (METH2) / Hs00199836_m1 / 59 / 605175
71 / COL18A1 (LOC51695 /endostatin) / Hs00181017_m1 / 72 / 120328
72 / FN1 (fibronectin) / Hs00415006_m1 / 86 / 135600
73 / HPSE (heparanase) / Hs00180737_m1 / 59 / 604724
74 / MMP2 / Hs00234422_m1 / 83 / 120360
75 / MMP9 / Hs00234579_m1 / 54 / 120361
76 / MSR1 / Hs00234012_m1 / 80 / 153622
77 / PLAU (uPA) / Hs00170182_m1 / 104 / 191840
78 / SERPINB5 (maspin) / Hs00184728_m1 / 104 / 154790
79 / SERPINF1 (PEDF) / Hs00171467_m1 / 88 / 172860
80 / THBS1 / Hs00170236_m1 / 109 / 188060
81 / THBS2 / Hs00170248_m1 / 87 / 188061
82 / THBS3 / Hs00200157_m1 / 85 / 188062
83 / THBS4 / Hs00170261_m1 / 98 / 600715
83 / TIMP1 / Hs00171558_m1 / 104 / 305370
85 / TIMP2 / Hs00234278_m1 / 73 / 188825
Transcription factors
86 / ERBB2 / Hs00397754_m1 / 58 / 164870
87 / ETS1 / Hs00428287_m1 / 92 / 164720
88 / HIF1A / Hs00153153_m1 / 76 / 603348
89 / ID1 / Hs00357821_g1 / 62 / 600349
90 / ID3 / Hs00171409_m1 / 129 / 600277
91 / MADH1 (SMAD1) / Hs00195432_m1 / 67 / 601595
Other related genes
92 / ENG (endoglin) / Hs00164438_m1 / 81 / 131195
93 / PTGS1 (cox-1) / Hs00168776_m1 / 109 / 176805
94 / PTGS2 (cox-2) / Hs00153133_m1 / 75 / 600262
95 / B2M (Housekeeping) / Hs99999907_m1 / 75 / 109700
96 / 18S (Housekeeping) / Hs99999901_s1 / 187 / 180473

Figure 1S. Representation of the genes clustered according to the expression profile in Nu499 series. 1, 24h; 2, 48h; 3, 72h; 4, 7 days; 5, 14 days; 6, 21 days; 7, 28 days

Figure 2S. Clusters of genes overexpressed in the early induction phase of angiogenesis in Os.

Figure 3S.Clusters of genes overexpressed in the late phase of angiogenesis in Os.

Figure 4S. Cluster analysis of qRT-PCR values of angiogenesis related genes from the Nu498 series. 1, 72h; 2, 7 days; 3, 14 days; 4, 21 days; 5, 28 days.

Table 2S.2 –ΔΔCt values corresponding to the Nu498 series.

#Gen / Nu498_72h / Nu498_1Week / Nu498_2Weeks / Nu498_3Weeks / Nu498_4Weeks
ADAMTS1-Hs00199608_m1_S18 / 0,2528443 / 0,82117695 / 0,81470597 / 2,0334287 / 0,2691712
ADAMTS8-Hs00199836_m1_S18 / 0,2956598 / 0,32457313 / 0,22372493 / 0,14256762 / 0,29363188
AMOT-Hs00611096_m1_S18 / 0,45903456 / 0,7208519 / 2,681206 / 2,2658222 / 2,152143
ANG;RNASE4-Hs00265741_s1_S18 / 8,249288 / 0,25597534 / 2,0186937 / 3,1229346 / 3,0276089
ANGPT1-Hs00181613_m1_S18 / 0,002358737 / 3,146703 / 0,001784849 / 0,035382744 / 0,084639296
ANGPT2-Hs00169867_m1_S18 / 67,87536 / 0,06960462 / 67,59587 / 80,797935 / 50,429253
CCL2-Hs00234140_m1_S18 / 0,7000301 / 0,7684878 / 0,52971077 / 0,3375556 / 0,6952286
CD36-Hs00169627_m1_S18 / 0,11922707 / 0,14044131 / 1,0722556 / 1,3264251 / 0,7498168
CDH5-Hs00174344_m1_S18 / 0,3723078 / 0,40871674 / 0,28172424 / 0,1795274 / 0,36975414
CHGA-Hs00154441_m1_S18 / 0,6752549 / 0,27146342 / 0,18711695 / 0,11923936 / 0,24558505
COL18A1-Hs00181017_m1_S18 / 54,75778 / 33,266933 / 98,40078 / 108,102356 / 78,9919
CSF3-Hs00236884_m1_S18
CXCL1-Hs00236937_m1_S18 / 6,5260634 / 3,4702835 / 0,089725375 / 0,057177056 / 0,1177617
EDG1-Hs00173499_m1_S18 / 0,03183312 / 0,034946162 / 0,02408803 / 0,015349979 / 0,031614773
EFNA2-Hs00154858_m1_S18 / 1,2972699 / 0,7953062 / 5,45675 / 6,006557 / 5,1641135
EFNA5-Hs00157342_m1_S18 / 1,3024133 / 3,3573036 / 1,9747531 / 1,0487205 / 2,2128365
EFNB2-Hs00187950_m1_S18 / 1,4487256 / 1,0058501 / 2,457351 / 3,5082765 / 1,0750633
EGF-Hs00153181_m1_S18 / 0,20307046 / 5,9368763 / 11,064665 / 17,812681 / 11,212972
EGFR-Hs00193306_m1_S18 / 1,5099069 / 1,0183932 / 0,40170434 / 2,968081 / 0,50867313
ENG-Hs00164438_m1_S18 / 0,13591056 / 0,33922833 / 0,40522322 / 0,18995972 / 0,2678195
EPHB4-Hs00174752_m1_S18 / 0,066120155 / 0,33386737 / 0,68921685 / 0,73822176 / 0,30804566
ERBB2;LEMD2-Hs00397754_m1_S18 / 0,5262931 / 0,4310972 / 1,236813 / 1,5610524 / 0,98056775
ETS1-Hs00428287_m1_S18 / 4,0980754 / 0,82816666 / 1,0802237 / 1,7614912 / 0,92458326
FGF1-Hs00265254_m1_S18 / 10,0348835 / 24,197826 / 9,603697 / 6,10986 / 4,598965
FGF2-Hs00266645_m1_S18 / 31,148443 / 78,21231 / 74,780205 / 65,53323 / 7,502213
FGF4-Hs00173564_m1_S18
FGF6-Hs00173934_m1_S18
FGF7-Hs00384281_m1_S18 / 19,419783 / 0,109266385 / 51,11911 / 22,649475 / 26,703878
FGFR1-Hs00241111_m1_S18 / 0,90178764 / 0,43991628 / 1,5931685 / 1,78715 / 1,9704136
FGFR2-Hs00240792_m1_S18 / 0,07316216 / 83,39237 / 0,0553616 / 0,035278905 / 0,072660334
FGFR3-Hs00179829_m1_S18 / 0,034143034 / 0,06808072 / 0,7546381 / 0,23238002 / 0,8172292
FGFR4-Hs00242558_m1_S18 / 3,4683433 / 2,9815001 / 3,7115436 / 3,8586833 / 5,7203135
FIGF-Hs00189521_m1_S18 / 0,236827 / 0,2599869 / 9,401513 / 2,1357033 / 0,2352026
FLT1-Hs00176573_m1_S18 / 2,6778903 / 0,037363525 / 40,059166 / 36,014824 / 29,905521
FLT4-Hs01047679_m1_S18
FN1-Hs00415006_m1_S18 / 1,279534 / 5,0248203 / 1,9232843 / 2,698059 / 1,5396378
HGF-Hs00300159_m1_S18
HIF1A-Hs00153153_m1_S18 / 68,02044 / 179,70808 / 171,7279 / 479,818 / 90,77654
HPSE-Hs00180737_m1_S18 / 0,4707119 / 0,72246623 / 1,5010974 / 1,4810514 / 1,2179512
ID1-Hs00357821_g1_S18 / 0,101003304 / 0,33381528 / 1,7146771 / 0,9510627 / 1,2346711
ID3-Hs00171409_m1_S18 / 0,14328718 / 0,36014968 / 1,3803668 / 1,340082 / 0,72255933
IFNA1-Hs00256882_s1_S18 / 304,8958 / 0,43510416 / 11,938021 / 5,5054893 / 64,84446
IFNB1-Hs00277188_s1_S18 / 30,374514 / 0,009098839 / 80,94441 / 153,97089 / 162,95206
IFNG-Hs00174143_m1_S18
IGF1-Hs00153126_m1_S18 / 0,018161627 / 0,17533731 / 0,3694209 / 0,11925244 / 0,25682482
IGF1R-Hs00181385_m1_S18 / 0,5508176 / 0,35817814 / 1,0594118 / 0,9521597 / 1,7027247
IL10-Hs00174086_m1_S18 / 11,704599 / 0,19266272 / 24,960928 / 17,059265 / 11,2537985
IL12A-Hs00168405_m1_S18 / 2,578584 / 2,898704 / 19,22195 / 13,9917755 / 4,591996
IL8-Hs00174103_m1_S18 / 59,684418 / 0,014802021 / 261,39822 / 307,4947 / 187,22829
ITGA5-Hs00233743_m1_S18 / 0,030676264 / 2,6978755 / 0,003814485 / 0,006141981 / 4,40E-04
ITGAV-Hs00233808_m1_S18 / 1,0325688 / 22,296722 / 0,00292291 / 0,00186261 / 0,003836227
ITGB3-Hs00173978_m1_S18 / 0,091974564 / 0,10096899 / 0,06959689 / 0,044350278 / 0,0913437
KDR-Hs00176676_m1_S18
MDK-Hs00171064_m1_S18 / 3,4063768 / 14,289084 / 0,7893094 / 0,52350134 / 0,9933589
MMP2-Hs00234422_m1_S18 / 0,6532832 / 0,4779472 / 1,8875905 / 1,7638075 / 1,3727441
MMP9-Hs00234579_m1_S18
MSR1-Hs00234012_m1_S18
NRP1-Hs00826129_m1_S18 / 0,16904086 / 0,47000733 / 0,92241853 / 0,71961594 / 0,40966722
PDGFA-Hs00234994_m1_S18 / 0,8563926 / 0,70679086 / 1,3561583 / 1,1343049 / 0,8257439
PDGFB-Hs00234042_m1_S18 / 13,295765 / 1,4577883 / 2,5597262 / 0,99968934 / 2,6778123
PDGFRA-Hs00183486_m1_S18 / 0,74602765 / 3,8307374 / 0,06776652 / 0,041868705 / 0,17260037
PDGFRB-Hs00182163_m1_S18 / 0,35608652 / 0,6151667 / 0,32236904 / 0,56005 / 0,22392201
PECAM1-Hs00169777_m1_S18 / 0,52836156 / 0,8711327 / 2,716623 / 2,5739722 / 2,5097592
PF4-Hs00236998_m1_S18
PGF-Hs00182176_m1_S18 / 0,2961876 / 0,07089874 / 1,1532228 / 1,4120799 / 0,9352224
PLAU-Hs00170182_m1_S18 / 22,2055 / 51,639698 / 5,7908816 / 10,787921 / 1,733138
PRL-Hs00168730_m1_S18 / 1,5606872 / 0,6076333 / 13,25999 / 8,919079 / 18,087223
PTGS1-Hs00168776_m1_S18 / 7,2630844 / 0,40264812 / 0,7843495 / 0,5640209 / 2,9781199
PTGS2-Hs00153133_m1_S18 / 1,5300086 / 0,3148757 / 1,4567239 / 1,981769 / 1,0324364
PTN-Hs00383235_m1_S18 / 0,707826 / 1,9435863 / 3,1492627 / 4,9738846 / 1,9279292
SERPINB5-Hs00184728_m1_S18 / 36,166794 / 39,038742 / 45,950993 / 73,699005 / 10,404619
SERPINF1-Hs00171467_m1_S18 / 1,9910142 / 0,5870076 / 6,0113077 / 6,4371257 / 7,8240395
SMAD1-Hs00195432_m1_S18 / 0,21942009 / 0,70047265 / 1,0991707 / 0,9963024 / 0,64597946
SPARC-Hs00277762_m1_S18 / 0,2579752 / 0,24368446 / 0,67971945 / 0,92219114 / 0,81697
TEK-Hs00176096_m1_S18 / 25,452593 / 47,175972 / 31,189116 / 17,954731 / 21,019323
TGFA-Hs00177401_m1_S18 / 1,3782952 / 0,17060913 / 0,20604171 / 0,33306894 / 0,6215621
TGFB1-Hs00171257_m1_S18 / 0,64226407 / 0,5440032 / 2,6546922 / 5,1895328 / 2,120355
TGFB2-Hs00234244_m1_S18 / 4,3023973 / 4,329787 / 14,111804 / 16,107138 / 10,469652
TGFB3-Hs00234245_m1_S18 / 0,28834724 / 0,30610308 / 1,0905582 / 0,2606725 / 0,40103203
TGFBR1-Hs00610319_m1_S18 / 0,4175003 / 0,81907994 / 1,4221501 / 1,8593204 / 1,0753064
TGFBR2-Hs00234253_m1_S18 / 0,5123231 / 0,5339583 / 0,7841131 / 0,76826024 / 1,256573
TGFBR3-Hs00234257_m1_S18 / 0,603101 / 1,2336317 / 0,32056743 / 0,79478383 / 0,36361784
THBS1-Hs00170236_m1_S18 / 0,047907837 / 5,4802246 / 0,053138368 / 0,08047663 / 0,023174893
THBS2-Hs00170248_m1_S18 / 0,613012 / 1,3006927 / 2,3625908 / 2,8123264 / 1,3408724
THBS3-Hs00200157_m1_S18 / 0,5003584 / 1,5914896 / 0,80823046 / 1,4973711 / 1,056064
THBS4-Hs00170261_m1_S18 / 2,469956 / 0,012840919 / 179,28757 / 103,70259 / 125,75335
TIE1-Hs00178500_m1_S18
TIMP1-Hs00171558_m1_S18 / 1,0067548 / 0,8611213 / 1,6609492 / 3,2215087 / 2,4167695
TIMP2-Hs00234278_m1_S18 / 0,67244774 / 0,37708268 / 0,7046496 / 1,1316073 / 0,80485135
TNF-Hs00174128_m1_S18 / 107,12509 / 0,4923384 / 0,3393638 / 0,21625791 / 0,4454042
TNFSF15-Hs00353710_s1_S18 / 4,9849443 / 0,057302676 / 0,003115261 / 0,18069132 / 0,531747
VEGFB-Hs00173634_m1_S18 / 1,711219 / 1,8128903 / 2,8469715 / 3,388294 / 5,3764677
VEGFC-Hs00153458_m1_S18 / 14,312813 / 0,37230635 / 85,53199 / 27,591024 / 60,403896
VEGF-Hs00173626_m1_S18 / 1,1307085 / 0,08270091 / 0,36202103 / 0,6611648 / 0,7782449

Table 3S.2 –ΔΔCt values corresponding to the Nu499 series.

#Gen / Nu499_24h / Nu499_48h / Nu499_72h / Nu499_1Week / Nu499_2Week / Nu499_3Week / Nu499_4Week
ADAMTS1-Hs00199608_m1_S18 / 3,0346258 / 0,10605343 / 0,87596476 / 0,21560732 / 1,2266936 / 2,8946867 / 3,4781654
ADAMTS8-Hs00199836_m1_S18 / 6,5725603 / 9,231163 / 0,2677915 / 0,2661837 / 0,22657055 / 0,18672575 / 0,23825057
AMOT-Hs00611096_m1_S18 / 2,8452895 / 0,43158764 / 0,17054823 / 0,049397428 / 0,65198976 / 0,75581026 / 0,6299683
ANG;RNASE4-Hs00265741_s1_S18 / 43,36105 / 83,39215 / 0,47589675 / 0,27854806 / 0,28858867 / 0,89893144 / 0,4110637
ANGPT1-Hs00181613_m1_S18 / 0,05243506 / 0,07364506 / 0,00213641 / 1,9908011 / 14,740737 / 38,08699 / 0,001900733
ANGPT2-Hs00169867_m1_S18 / 242,5968 / 32,698246 / 1,6804082 / 0,09305665 / 2,8429732 / 6,866021 / 2,170476
CCL2-Hs00234140_m1_S18 / 15,5617695 / 21,856512 / 8,611019 / 0,63023984 / 9,027306 / 132,98067 / 25,7975
CD36-Hs00169627_m1_S18 / 0,5349101 / 0,02133026 / 0,11577508 / 0,14318928 / 0,8237213 / 1,4558219 / 2,143403
CDH5-Hs00174344_m1_S18 / 8,276456 / 11,624286 / 5,525476 / 0,33519018 / 0,28530756 / 1,9666451 / 4,450557
CHGA-Hs00154441_m1_S18 / 5,497096 / 7,720673 / 0,22397293 / 0,22262822 / 0,18949693 / 0,15617193 / 9,633122
COL18A1-Hs00181017_m1_S18 / 943,2587 / 433,7997 / 0,3820556 / 0,022792373 / 0,019400436 / 0,015988668 / 0,39392385
CSF3-Hs00236884_m1_S18
CXCL1-Hs00236937_m1_S18 / 45,094357 / 3,7021782 / 29,773678 / 0,10675356 / 4,528039 / 28,209433 / 9,489852
EDG1-Hs00173499_m1_S18 / 0,70765483 / 0,99390143 / 1,2170047 / 0,53329796 / 9,642436 / 38,174404 / 52,3916
EFNA2-Hs00154858_m1_S18 / 3,234444 / 4,5141363 / 0,09616797 / 0,043672074 / 0,04870536 / 0,19884154 / 0,74292433
EFNA5-Hs00157342_m1_S18 / 1,873053 / 0,11197063 / 0,3597314 / 0,5336097 / 0,81825304 / 1,1165826 / 2,011615
EFNB2-Hs00187950_m1_S18 / 3,508601 / 1,6092389 / 0,82286906 / 0,5016427 / 0,7745507 / 0,810214 / 1,4625199
EGF-Hs00153181_m1_S18 / 171,53163 / 6,3403153 / 0,18392943 / 0,18282513 / 0,1556173 / 0,12825038 / 0,16363959
EGFR-Hs00193306_m1_S18 / 12,190401 / 0,16698776 / 1,6590575 / 0,121798985 / 0,5214491 / 1,3918917 / 5,605011
ENG-Hs00164438_m1_S18 / 0,1834047 / 0,25759196 / 3,1708117 / 1,6425493 / 7,774633 / 12,881164 / 13,228594
EPHB4-Hs00174752_m1_S18 / 2,5452256 / 1,8418201 / 0,64983064 / 0,24744502 / 1,256754 / 2,3815608 / 2,3034258
ERBB2;LEMD2-Hs00397754_m1_S18 / 5,6592402 / 3,7764091 / 0,18721741 / 0,12067301 / 0,37895378 / 0,73706615 / 0,9936649
ETS1-Hs00428287_m1_S18 / 4,5872636 / 1,248847 / 0,3052189 / 0,26802456 / 0,83943796 / 1,5521703 / 1,7801948
FGF1-Hs00265254_m1_S18 / 71,90395 / 26,239058 / 1,9191124 / 0,61134636 / 0,31755355 / 0,60093373 / 0,6294122
FGF2-Hs00266645_m1_S18 / 60,101395 / 4,1154275 / 0,11938653 / 0,11866974 / 0,10100944 / 0,08324588 / 0,10621662
FGF4-Hs00173564_m1_S18
FGF6-Hs00173934_m1_S18
FGF7-Hs00384281_m1_S18 / 28,735283 / 3,1076381 / 1,2752604 / 0,089609794 / 0,076274164 / 0,062860556 / 15,205117
FGFR1-Hs00241111_m1_S18 / 5,5194125 / 1,2900755 / 0,13489993 / 0,09550096 / 0,3266716 / 0,7216362 / 0,8960321
FGFR2-Hs00240792_m1_S18 / 1,6264054 / 2,284287 / 0,06626604 / 5,016308 / 8,593028 / 9,591477 / 0,05895602
FGFR3-Hs00179829_m1_S18 / 2,2793665 / 0,14302184 / 0,07815248 / 0,10065458 / 0,28218052 / 0,45981443 / 1,1072934
FGFR4-Hs00242558_m1_S18 / 145,82663 / 48,451797 / 0,11697314 / 0,11627084 / 0,098967545 / 1,3417555 / 0,10406946
FIGF-Hs00189521_m1_S18 / 282,13458 / 7,3942704 / 0,21450415 / 0,21321629 / 0,18148568 / 0,14956954 / 5,9169188
FLT1-Hs00176573_m1_S18 / 24,349041 / 1,0626535 / 0,03082706 / 0,030641973 / 0,02608187 / 0,0214951 / 1,0309292
FLT4-Hs01047679_m1_S18
FN1-Hs00415006_m1_S18 / 0,68746364 / 0,03979574 / 0,15168546 / 2,3996913 / 1,3722814 / 2,877446 / 3,7816677
HGF-Hs00300159_m1_S18
HIF1A-Hs00153153_m1_S18 / 432,7354 / 37,56903 / 0,10919837 / 13,385185 / 95,42428 / 262,3812 / 0,010167615
HPSE-Hs00180737_m1_S18 / 1,3731843 / 1,0284108 / 0,5339089 / 0,7098709 / 0,78798103 / 0,73891896 / 1,2239236
ID1-Hs00357821_g1_S18 / 1,4935987 / 0,64747834 / 0,10694482 / 0,078515984 / 0,60467625 / 1,3770676 / 1,8931288
ID3-Hs00171409_m1_S18 / 0,6806416 / 0,2506692 / 0,0877308 / 0,087093465 / 0,87878805 / 1,7187988 / 2,7067678
IFNA1-Hs00256882_s1_S18 / 2593,1782 / 5505,0664 / 152,95665 / 27,339174 / 6,5773325 / 9,161485 / 9,556151
IFNB1-Hs00277188_s1_S18 / 142,96265 / 40,340206 / 6,8598876 / 0,007461994 / 0,0770653 / 0,16048558 / 207,08243
IFNG-Hs00174143_m1_S18
IGF1-Hs00153126_m1_S18 / 0,40373555 / 0,5670467 / 0,01644975 / 0,016350985 / 0,99495584 / 1,158815 / 2,8692462
IGF1R-Hs00181385_m1_S18 / 3,099858 / 1,0237159 / 0,12408141 / 0,10778242 / 0,22134335 / 0,5684376 / 0,61286294
IL10-Hs00174086_m1_S18 / 90,1514 / 13,991313 / 1,0007023 / 0,009410593 / 0,008010119 / 0,006601456 / 6,0688424
IL12A-Hs00168405_m1_S18 / 8,415258 / 0,2893294 / 0,22540593 / 0,10325935 / 0,12556382 / 0,317877 / 0,14582974
IL8-Hs00174103_m1_S18 / 321,19354 / 51,42421 / 18,83577 / 0,42357203 / 0,2148047 / 4,870697 / 126,30786
ITGA5-Hs00233743_m1_S18 / 0,00985174 / 0,01383678 / 0,0304209 / 0,1401151 / 0,08047152 / 0,13717726 / 0,5692829
ITGAV-Hs00233808_m1_S18 / 0,08586885 / 1,6259732 / 0,2150448 / 1,946595 / 11,247596 / 21,50951 / 0,16089007
ITGB3-Hs00173978_m1_S18 / 34,04387 / 2,8716524 / 21,275448 / 0,08280507 / 0,070482105 / 0,058087096
KDR-Hs00176676_m1_S18
MDK-Hs00171064_m1_S18 / 18,850344 / 1,6151536 / 0,04685481 / 2,4911675 / 25,427837 / 82,12333 / 0,041686106
MMP2-Hs00234422_m1_S18 / 2,2604635 / 0,44397265 / 0,26280826 / 0,1826519 / 0,46909973 / 1,4020625 / 1,3211833
MMP9-Hs00234579_m1_S18
MSR1-Hs00234012_m1_S18
NRP1-Hs00826129_m1_S18 / 0,8551391 / 1,1436121 / 0,26467407 / 0,14985456 / 1,3923131 / 4,0362988 / 3,844263
PDGFA-Hs00234994_m1_S18 / 6,728114 / 1,5496442 / 0,25729892 / 0,27653146 / 0,49828887 / 0,81763774 / 1,1746796
PDGFB-Hs00234042_m1_S18 / 115,961494 / 30,349266 / 0,14130773 / 0,07502362 / 0,025564661 / 0,08898303 / 0,05084654
PDGFRA-Hs00183486_m1_S18 / 6,6930704 / 9,936688 / 0,3811853 / 2,7906003 / 23,098297 / 74,11025 / 0,12459544
PDGFRB-Hs00182163_m1_S18 / 0,9719293 / 0,10464464 / 0,95761216 / 0,2551334 / 0,30979 / 0,893389 / 4,7108974
PECAM1-Hs00169777_m1_S18 / 4,3440866 / 0,10022554 / 0,40089792 / 0,15245438 / 1,2034018 / 0,8567165 / 1,2354548
PF4-Hs00236998_m1_S18
PGF-Hs00182176_m1_S18 / 2,6469784 / 0,8941606 / 0,38959396 / 0,2771672 / 1,0132589 / 2,431115 / 3,5605214
PLAU-Hs00170182_m1_S18 / 21,501635 / 4,1609235 / 0,76123756 / 0,4384731 / 0,30826974 / 0,27868178 / 0,35086152
PRL-Hs00168730_m1_S18 / 8,792563 / 1,2820647 / 0,03719207 / 0,036968768 / 0,031467117 / 0,025933295 / 0,033089288
PTGS1-Hs00168776_m1_S18 / 16,313002 / 10,806335 / 0,01905879 / 0,96072024 / 0,016125081 / 0,013289318 / 0,01695635
PTGS2-Hs00153133_m1_S18 / 1,5608925 / 0,34267372 / 0,247803 / 4,91E-04 / 3,59E-05 / 2,96E-05 / 2,1633184
PTN-Hs00383235_m1_S18 / 4,0489655 / 0,4052302 / 2,59E-04 / 2,58E-04 / 0,050888974 / 0,36236292 / 0,7609055
SERPINB5-Hs00184728_m1_S18 / 1,3729701 / 1,928337 / 0,05594011 / 0,055604246 / 0,047329284 / 0,03900594 / 0,049769174
SERPINF1-Hs00171467_m1_S18 / 7,5053973 / 1,0178467 / 0,01289333 / 0,015296537 / 0,19533314 / 0,40054455 / 0,9277497
SMAD1-Hs00195432_m1_S18 / 1,0038838 / 0,39802393 / 0,04464513 / 0,030896705 / 0,2354406 / 0,45508447 / 1,7102442
SPARC-Hs00277762_m1_S18 / 1,4504839 / 0,15996544 / 0,09991735 / 0,18355498 / 0,89741564 / 2,3789568 / 2,1173296
TEK-Hs00176096_m1_S18 / 2,8962715 / 2,8385115 / 0,08234382 / 0,08184943 / 0,069668695 / 0,05741673 / 0,07326021
TGFA-Hs00177401_m1_S18 / 3,3575654 / 4,7029014 / 2,9306097 / 8,119307 / 6,3853874 / 4,5370755 / 6,797136
TGFB1-Hs00171257_m1_S18 / 3,2874446 / 0,43247125 / 0,09280368 / 0,06566837 / 0,24904893 / 0,5965082 / 0,68560344
TGFB2-Hs00234244_m1_S18 / 9,41349 / 1,1640803 / 0,06345037 / 0,030925557 / 0,049803138 / 0,046021283 / 0,01970135
TGFB3-Hs00234245_m1_S18 / 1,0284978 / 0,02187551 / 0,08184165 / 0,0701087 / 0,21028553 / 0,36372027 / 1,0885113
TGFBR1-Hs00610319_m1_S18 / 0,41152373 / 0,05866655 / 0,37241513 / 0,31072396 / 1,0487441 / 1,6810548 / 1,2086396
TGFBR2-Hs00234253_m1_S18 / 2,394448 / 0,8703165 / 0,3361052 / 0,09890777 / 0,16341755 / 0,18945059 / 1,2589042
TGFBR3-Hs00234257_m1_S18 / 7,81504 / 0,4672367 / 1,1197003 / 0,5942036 / 0,58358043 / 2,9371145 / 2,074872
THBS1-Hs00170236_m1_S18 / 0,09627967 / 0,00429042 / 0,00925844 / 0,9824831 / 2,0469067 / 3,9376762 / 0,22891423
THBS2-Hs00170248_m1_S18 / 5,4325852 / 1,3444138 / 0,33937725 / 0,29656532 / 0,22819899 / 0,36680987 / 0,26264223
THBS3-Hs00200157_m1_S18 / 1,6495981 / 0,05739381 / 0,32136393 / 0,00165497 / 0,50546604 / 1,7791407 / 2,7443905
THBS4-Hs00170261_m1_S18 / 43,680843 / 0,36520773 / 6,2702017 / 0,35802382 / 0,008963693 / 0,89206713 / 179,40588
TIE1-Hs00178500_m1_S18
TIMP1-Hs00171558_m1_S18 / 2,3056257 / 0,30926904 / 0,21121532 / 0,23892622 / 0,3716041 / 0,8442103 / 1,0430279
TIMP2-Hs00234278_m1_S18 / 3,4110458 / 0,494378 / 0,1681335 / 0,1114512 / 0,5560329 / 0,94694614 / 1,1408893
TNF-Hs00174128_m1_S18 / 9,969783 / 14,002563 / 0,4062075 / 6,868699 / 6,2732368 / 0,2832405 / 0,36139745
TNFSF15-Hs00353710_s1_S18 / 42,235855 / 70,530754 / 1,6231017 / 0,47810394 / 1,2870712 / 3,2298887 / 5,7458715
VEGFB-Hs00173634_m1_S18 / 6,7702303 / 2,8928273 / 0,59828365 / 1,1729168 / 1,6778287 / 2,5556548 / 0,804562
VEGFC-Hs00153458_m1_S18 / 50,81656 / 8,334465 / 6,225342 / 0,036051366 / 0,20050594 / 0,46376202 / 16,266634
VEGF-Hs00173626_m1_S18 / 12,892732 / 8,56892 / 1,3774518 / 1,0384885 / 0,13769656 / 0,2111338 / 0,41907746