Supplementarymaterials

Supplementary Table 1 Expression profiles of the identified AOS sequences in soybean fourteen tissues*

GmAOS1
Glyma14g08560 / GmAOS2
Glyma17g36530 / GmAOS3
Glyma04g03740
young_leaf / 3 / 5 / 0
flower / 13 / 7 / 3
one cm pod / 12 / 5 / 1
pod shell 10DAF / 16 / 11 / 0
pod shell 14DAF / 22 / 28 / 2
seed 10DAF / 5 / 2 / 0
seed 14DAF / 25 / 6 / 0
seed 21DAF / 23 / 8 / 0
seed 25DAF / 23 / 8 / 0
seed 28DAF / 11 / 5 / 0
seed 35DAF / 15 / 9 / 0
seed 42DAF / 8 / 9 / 0
root / 17 / 20 / 1
nodule / 9 / 30 / 0

*: The data were obtained by mining the SoySeq database (

Supplementary Table 2 Sequence polymorphism of GmAOS1

Name of Loci / SNP/
Indel / Polymorphism / Name of Loci / SNP/
Indel / Polymorphism / Name of Loci / SNP/
Indel / Polymorphism
S10 / Indel / 0/5 / S762 / SNP / A/G / S840 / SNP / A/G
S73 / SNP / A/G / S767 / SNP / C/T / S843 / SNP / A/G
S201 / SNP / A/G / S770 / SNP / C/G / S844 / SNP / G/T
S657 / SNP / A/G / S772 / SNP / A/C / S850 / SNP / A/G
S684 / SNP / C/G / S773 / SNP / A/G / S854 / SNP / C/G
S696 / Indel / 0/11/32 / S776 / SNP / A/G / S855 / SNP / A/G
S697 / SNP / A/G / S777 / SNP / A/C / S861 / SNP / C/G
S719 / SNP / A/G / S779 / Indel / 0/2 / S862 / Indel / 0/5
S732 / SNP / C/T / S781 / SNP / C/T / S871 / SNP / C/T
S734 / SNP / C/T / S814 / SNP / A/C/G / S887 / SNP / C/G
S739 / SNP / A/G/T / S820 / SNP / A/G / S971 / SNP / A/C
S741 / SNP / A/G / S822 / SNP / A/G / S1008 / SNP / A/C
S746 / SNP / A/G / S824 / Indel / 0/8 / S2031 / SNP / C/T
S748 / SNP / A/T/G / S833 / SNP / C/T
S749 / SNP / A/T / S838 / SNP / A/C

Note: Indelstands for insertions/deletions. SNP stands for single nucleotide polymorphism. The letters A, C, G and T in the third column stand for four nucleobases (adenine, cytosine, guanine and thymine). The number in the third column stands for the nucleic acid number of an Indel.

Supplementary Table 3 Alleles and phenotype values of GmAOS1

Name of allele / Number / ELG (Mean±SD) / RSN (Mean±SD) / RSW (Mean±SD)
GmAOS1_1 / 33 / 0.223±0.050 / 0.660±0.276 / 0.631±0.296
GmAOS1_2 / 30 / 0.229±0.048 / 0.674±0.197 / 0.657±0.212
GmAOS1_3 / 1 / 0.213 / 0.400 / 0.340
GmAOS1_4 / 10 / 0.224±0.054 / 0.734±0.251 / 0.727±0.267
GmAOS1_5 / 1 / 0.305 / 0.480 / 0.54
GmAOS1_6 / 23 / 0.208±0.043 / 0.746±0.348 / 0.737±0.378
GmAOS1_7 / 4 / 0.183±0.057 / 0.765±0.55 / 0.790±0.561
GmAOS1_8 / 1 / 0.194 / 1.290 / 1.420
GmAOS1_9 / 1 / 0.244 / 0.630 / 0.620
GmAOS1_10 / 1 / 0.230 / 1.230 / 1.270
GmAOS1_11 / 2 / 0.259±0.032 / 0.770±0.071 / 0.780±0.028
GmAOS1_12 / 3 / 0.189±0.024 / 0.453±0.238 / 0.477±0.180
GmAOS1_13 / 1 / 0.136 / 0.540 / 0.520
GmAOS1_14 / 1 / 0.279 / 0.570 / 0.610
GmAOS1_15 / 1 / 0.230 / 1.810 / 1.880
GmAOS1_16 / 2 / 0.219±0.043 / 0.89±0.127 / 0.88±0.148
GmAOS1_17 / 23 / 0.243±0.044 / 0.717±0.380 / 0.695±0.425
GmAOS1_18 / 1 / 0.266 / 1.200 / 1.120
GmAOS1_19 / 1 / 0.068 / 0.530 / 0.530
GmAOS1_20 / 1 / 0.218 / 0.710 / 0.770
GmAOS1_21 / 10 / 0.208±0.054 / 1.009±0.271 / 1.032±0.268
GmAOS1_22 / 17 / 0.228±0.039 / 0.611±0.108 / 0.598±0.129
GmAOS1_23 / 1 / 0.136 / 0.580 / 0.560
GmAOS1_24 / 4 / 0.200±0.061 / 0.765±0.304 / 0.733±0.294
GmAOS1_25 / 1 / 0.169 / 0.690 / 0.660
GmAOS1_26 / 9 / 0.217±0.052 / 0.635±0.198 / 0.571±0.172
GmAOS1_27 / 1 / 0.137 / 0.610 / 0.600

Note: ELG stands for effect of leaves onlarvae growth which was defined as the live larvae weights after feeding divided bythe total weights of leaves consumed over 7 days and was used as the index parameter for insect bioassays.

RSN was defined as the ratios of treatment to control for seed number per plant. RSW was defined as the ratios of treatment to control for seed weight per plant. RSN and RSW were used as the index parameters for soybean regrowth after mimicking common cutworm attack.

SD stands for standard deviation.

SupplementaryFig. 1 Cis-acting regulatory elements analysis of promoter sequences of GmAOS1_21by SoftBerry-TSSP (Shahmuradov et al. 2003) and PlantCARE (Lescot et al. 2002)

SoftBerry-TSSP( was used to predict the position of promoterand PlantCARE was used to predict cis-regulating elementsof promoter.GmAOS1_21 was used as an example to display the predicted cis-regulating elementsof presumptive promoter. Each motif was underline and the name was list below the motif. Two elements (TATCCAT/C and Box II–like sequence) were unique to GmAOS1_21 and were shown in bold type.

Supplementary Fig.2Neighbor-joining tree of 27 GmAOS1 alleles

The sequences of 27 GmAOS1 alleles were aligned by Clustal W in MEGA 5.0.The neighbor-joining tree was constructed by MEGA 5.0with the following parameters: p-distance, pairwise deletion, and bootstrap (1000 replications, random seed).