WORD LIST
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AGAR
ASEPTIC TECHNIQUE
AUTOCLAVE
BLUNT
BODY
BROTH
COLONY
CONTAMINATION
CULTIVATION
CULTURE
DIFFERENTIAL
ENRICHED
FLAMING
FORTY FIVE
GELIDIUM
GENERAL PURPOSE
INCUBATION
INCUBATOR
INOCULATING LOOP
INOCULUM
INVERTED
ISOLATION
MEDIUM
MIXED
MORPHOLOGY
ONE HUNDRED
OPTIMUM
PLATE
POUR PLATE
PURE
REDUCES
ROOM
SELECTIVE
SLANT
STERILIZED
STREAK PLATE
SYNTHETIC
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THE CULTIVATION OF MICROORGANISMS
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ACROSS
1.Following inoculation media is generally placed in an insulated cabinet at a specific temperature; this cabinet is called an ______.
3.Separating an individual genus microorganism from a mixed culture.
6.The observable characteristics of a bacterial colony are referred to as the colony ______.
7.Media which provides the basic nutrients required for the growth of most microbes is called ______media.
8.Media which enables one to identify an organism based on its biochemical (metabolic) reactions is called ______media.
10.A clump of bacteria growing on the surface of a solid medium which are descendants of a single cell is called a ______.
11.A culture consisting of only one species of organism is called a ______culture.
12.Method of isolation in which an inoculum is aseptically spread over the surface of a solid medium is called the ______method.
13.The growth of microbes in a medium is called ______.
15.The procedure used to prevent the introduction of unwanted organisms into culture media is referred to as the ______.
16._____ temperature is 37oC.
18.The introduction of unwanted organisms into culture media is referred to as ______.
20._____ temperature is 20 o - 25o C.
22.Media composed of specific quantities of definite chemical components is referred to as ______media.
24.A liquid growth medium is commonly referred to as a ______.
26.An agar _____ is prepared by pouring a liquid agar medium into a petri dish allowing it to solidify.
28.Following inoculation, media must be held at a specific temperature that promotes maximum growth of the microbes; this is called the ______period.
30.Agar is extracted from this genus algae.
32.Passing an inoculating loop through the flame of a Bunsen burner in order to destroy any microbes present is referred to as ______.
DOWN
1.Agar plates should be stored/incubated in this position to prevent moisture from collecting on the surface of the medium.
2.The solidifying agent used for the preparation of solid media.
4.After powdered media is hydrated it must be ______to prevent microbial growth.
5.Agar liquefies when heated to a temperature of ______degrees C.
6.A ______culture contains many types of organisms.
9.The instrument commonly used to transfer an inoculum to a slide or fresh medium is called the ______.
12.A medium which will support the growth of only one specific organism is called a ______medium.
13.Growing organisms on artificial media in the laboratory is referred to as ______.
14The ______method of isolation involves adding the inoculum to liquid agar and then pouring the mixture into a petri dish.
17.A general purpose medium containing special metabolites (blood, serum, etc.) is called an ______medium.
19 Media is commonly sterilized with steam under pressure in an apparatus called the ______.
21.The sample of organisms introduced or transferred to fresh media is called the ______.
22.If melted agar medium is put into a test tube and allowed to solidify at an angle, the medium is referred to as a ______.
23.Liquid agar will solidify when it reaches a temperature of about ______degrees C.
25.The temperature at which an organism grows best is called the ____ temperature.
27.A substance that provides the nutrients needed for the growth of microorganisms is called a culture ______.
29.In the streak plate method of isolation flaming the loop after streaking each section of the plate ______the number of organisms.
31.An agar _____ is prepared by putting melted agar into a test tube which is allowed to cool and solidify in an upright position.
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