Tables5: List of EST-SSR Primers Used for Characterization of Tinospora Cordifolia Accessions

TableS5: List of EST-SSR primers used for characterization of Tinospora cordifolia accessions.

S.No. / Contig /transcript ID / Primer / Forward Primer Sequence
(5'-3') / Reverse Primer Sequence
(5'-3') / Tm (°C) / Ta (°C) / Expected Product Size (bp) / Amplified Product Size (bp) / No. of alleles amplified
1 / contig00235 / Giloe SSR1** / ACCACCAACGATAGCAAC / TTCTGAAGATGGGTTTGTATG / 54 / 52 / 148 / 310 / 1
2 / contig00235 / Giloe SSR2** / GGTGGATTTGGTAAACAAGAC / TCTTCTTGTTTTCTGCTTCTG / 55.9 / 52 / 163 / 320 / 1
3 / contig04775 / Giloe SSR3** / CAGGATACAATTCTTCAACCA / TAGGAGCAAGTACGAGTCAAC / 54 / 52 / 154 / 200, 320 / 2
4 / contig08355 / Giloe SSR4 / ACACGGGACTCTCTTTATGAT / TATTAATTCTCCCAATTCCAG / 55.9 / 56 / 159 / 173, 175 / 2
5 / contig06427 / Giloe SSR5 / CAATCTCAGCACCAATTTTTA / ATCATAAAGAGAGTCCCGTGT / 52 / 52 / 170 / 350, 510 / 2
6 / contig00062 / Giloe SSR6 / CTCATTTTCTGCACAGTTACC / AGTAAAGATGGAAGGAAAAGC / 55.9 / 56 / 150 / 165, 168 / 2
7 / contig04428 / Giloe SSR7 / ATGACCAGAACAAGATGCTTA / GGCGTCTGAATTTAGGATTAC / 54 / 52 / 151 / 108, 115, 300 / 3
8 / contig04428 / Giloe SSR8** / TCAAGAACTGGCTCTCAAAT / TTCTTGTTGGATTCTGAACAT / 53.2 / 52 / 258 / 310 / 1
9 / contig06000 / Giloe SSR9** / TTGATAGAAAGGGCTACAAAA / AATATGAAACATTGCATGTCC / 52 / 53 / 171 / 185 / 1
10 / contig00812 / Giloe SSR10 / TTCACCAAGTAATCCAAAAGA / TGGTAGAAAACACAGTCCTCA / 52 / 52 / 142 / 295 / 1
11 / contig05537 / Giloe SSR11 / ACATGTGCAAAATAAGTGTGTT / TACAGATGCTGGGAAAAACT / 52.8 / 52 / 151 / 115, 300, 350 / 3
12 / contig00073 / Giloe SSR12 / CAGCTAAAGCTAAGCTCATTG / TGATTTCCTCAAATTGGAAC / 55.9 / 53 / 139 / 150, 160 / 2
13 / contig00116 / Giloe SSR13 / GTTTTTGGAGGAATCACTCA / GCTATAACCGAAGCATGTAAA / 53.2 / 56 / 148 / 165 / 1
14 / contig00123 / Giloe SSR14** / AAGAAAACTAGGGCGGATAC / CCTTCACACCTTGAAGACTAT / 55.3 / 53 / 152 / 160, 180 / 2
15 / contig00123 / Giloe SSR15 / CTAGATTTGTATTTCCATAATGC / TCACTCATTATCCTGTTGAGG / 53.5 / 52 / 150 / 300, 360 / 2
16 / contig00034 / Giloe SSR16 / AAGGATGTCATTGGCAGTAG / ACTTGGGACTTCATAACCATT / 55.3 / 52 / 150 / 350 / 1
17 / contig00034 / Giloe SSR17 / TATGAAGTCCCAAGTTTTCAA / ACTGTATCCTCTTCCCTGATG / 52 / 52 / 167 / 115, 315 / 2
18 / contig00142 / Giloe SSR18 / CTCATCGTCAGAGAGAATCAG / TATGCCATAATGGTCACATCT / 57.9 / 56 / 145 / 160 / 1
19 / contig00142 / Giloe SSR19 / CAAGCAATATCATTTTCAGGA / TGAGCCTGTTACTGGTAGAGA / 52 / 53 / 156 / 155, 170 / 2
20 / contig00158 / Giloe SSR20 / CTGCTGCAAAATCAAGATAGT / GTCGGGTTGGAGTTTGAT / 54 / 56 / 157 / 175, 200, 300 / 3
21 / contig00306 / Giloe SSR21** / TTCTCTGATCTTCAGGTTTCC / GTTGTTGTTGTTGTTGTTGC / 55.9 / 56 / 151 / 160 / 1
22 / contig00306 / Giloe SSR22 / AATATCGGTGTAGCTCAAGTG / ACAATAAACACCATCGTCAAC / 55.9 / 56 / 186 / 190 / 1
23 / contig00306 / Giloe SSR23 / GTGGTCCAACAACAACAATAG / ACAATAAACACCATCGTCAAC / 55.9 / 56 / 154 / 140, 170 / 2
24 / contig00181 / Giloe SSR24 / GGAGGTTTTTGAAACTTTAGC / AATTCCACTCCAACTGTATCC / 54 / 53 / 162 / 175 / 1
25 / contig00193 / Giloe SSR25 / AGGAAACTGTCTCCAGTGTTA / TCATGACCTTACTCTGTCTCC / 55.9 / 55 / 160 / 298 / 1
26 / contig00193 / Giloe SSR26** / CAGATGCAAGTCAATCATTTT / ATTGCATCTTCCATTGACATA / 52 / 55 / 162 / 270 / 1
27 / contig00223 / Giloe SSR27** / AATTCCGAAAATCAAAGAGAG / GAGCAGTATGATAGCAGCATT / 52 / 55 / 143 / 155 / 1
28 / contig00235 / Giloe SSR28** / AATTGGTCCTCCAGATTTAAG / GGAATCAGAAGAAGATGATGA / 54 / 56 / 154 / 150 / 1
29 / contig00235 / Giloe SSR29 / CACCAATTAGACGCTTTGTAT / GATTGATCGCTTCCATAGTC / 54 / 56 / 154 / 160, 180 / 2
30 / contig00266 / Giloe SSR30 / AGAACCTATGGATTTCCAGTC / CTAACGAGCGTCTGTTTCTTA / 55.9 / 56 / 169 / 185, 200 / 2
31 / contig00269 / Giloe SSR31 / CTCACACTCACATTTCTTTGC / CGAAGTGATGAAAGCATTAGT / 55.9 / 52 / 145 / 160 / 1
32 / contig00269 / Giloe SSR32 / ATCATTCTCCAAAGACGAAAC / CCTTTCAGAAGAAGAAACGAT / 54 / 53 / 143 / 200, 250, 400 / 3
33 / contig03504 / Giloe SSR33 / GCCCTTTCTTTACCTCAATAG / GAACACACCTTATGTTTCAGG / 55.9 / 52 / 158 / 110, 315 / 2
34 / contig03664 / Giloe SSR34 / GGACTGAATTTCCAAAGAGAG / TCGACTAGTGCAGTCTTCTTC / 55.9 / 52 / 147 / 110, 155 / 2
35 / contig03664 / Giloe SSR35 / ATGTCGGAAAACTAGGTCATT / GCATTGATTTAAACTTTGCAG / 54 / 52 / 152 / 160 / 1
36 / contig04994 / Giloe SSR36 / GTGGATTAGGGCATCAGTTAT / CTGCATTGCTTTAAAAGAGAG / 55.9 / 56 / 144 / 150, 185 / 2
37 / contig05510 / Giloe SSR37 / CAGTTTCTTTCACTCATCCAA / GATGATGAACATGAATTGTGA / 54 / 56 / 145 / 155, 170 / 2
38 / contig05002 / Giloe SSR38 / GTCCCCTTAACGTTCATAATC / TTCCATTCCATTTTACATCTG / 55.9 / 52 / 158 / 190, 200 / 2
39 / contig05670 / Giloe SSR39 / TGGTGGGTGTTAGAGTTAGTT / CCAGCTTGTCTTAAGTTGATG / 55.9 / 56 / 189 / 198 / 1
40 / contig05703 / Giloe SSR40** / TAACCGAAGACAATTACCTGA / GTTATGCCCGTGAGAAAAT / 54 / 56 / 157 / 170 / 1
41 / Tinospora-est-c207 / Giloe SSR41 / AAGTAGAGGCAAATGAAATCTT / TCCTATTTTTCCTTCATTTC / 52.8 / 52 / 261 / 215, 315 / 2
42 / Tinospora-est-c207 / Giloe SSR42** / AATTTCAAAAGCTCTTCTCGT / TTCTGTCCGCTAGAATTTATG / 52 / 56 / 134 / 145 / 1
43 / contig07103 / Giloe SSR43 / TTCTTCACCTATGGATGTGAG / TGGAAGATATTTCATTCATGC / 55.9 / 52 / 166 / 160 / 1
44 / contig04390 / Giloe SSR44 / AGACGAGAACAGCAAAGAAG / ACCATCAGATTAACAGAACCA / 55.3 / 56 / 181 / 215, 315, 355 / 3
45 / contig12827 / Giloe SSR45** / GAGGTTTCCTCTGAAAAACTG / TTCCTAGAGAAGGAAAGGACT / 55.9 / 56 / 142 / 300 / 1
46 / contig13108 / Giloe SSR46 / AAAACAAAATATGGGCACAC / GTTTTCTTTTCTAGCGCTGT / 51.2 / 56 / 160 / 160, 215 / 2
47 / contig11860 / Giloe SSR47 / GCTTAGAATGGCTATCAATCTT / AGGGTTTTTGGATGTGAAG / 56.6 / 51.9 / 170 / 170, 200, 240 / 3
48 / contig01072 / Giloe SSR48 / AAGATGGGGTACAGGAAGAT / GAAGAAGAAGAAATTAACAATGG / 56.4 / 51.9 / 159 / 150, 165, 180 / 3
49 / contig04523 / Giloe SSR49 / TTCAAGAGCCTAGATTGGAA / CTTGGACTTTCCCTCTTTTT / 54.3 / 53 / 169 / 160, 175, 400 / 3
50 / contig08246 / Giloe SSR50 / TTTGATAGTCTATGCCAGTCC / CGTTTCTTTAAAAACCAAAAA / 57.4 / 53 / 153 / 145, 170, 180, 195 / 4
51 / Tinospora-est-c18089 / Giloe SSR51 / AAATCCAAAGGCAACTTTCT / TTTCCAACAAAAGATTACGG / 52.3 / 53 / 154 / 140, 150 / 2
52 / contig11278 / Giloe SSR52 / GAATTCCACGAGAATCACC / AAGAAGAAGAAGAAGAGTGAGC / 55.2 / 51.9 / 163 / 170, 180, 190 / 3
53 / contig08247 / Giloe SSR53 / TTTTTCTTTCCTTTTTCTTCC / TTTTCTCATCCCATTCTCAC / 51.6 / 53 / 160 / 160, 170 / 2
54 / contig01727 / Giloe SSR54 / TTTTCTTTTGTCCGTGATTT / TGCGTACACGTTACAATTAAA / 50.2 / 53 / 303 / 300, 395 / 2
55 / contig09533 / Giloe SSR55 / TCTGGTTCTCTTGGCTTTAC / ACCTTCTGAACAAAATGATGA / 56.4 / 53 / 150 / 140, 150, 160 / 3
56 / contig09834 / Giloe SSR56 / AGCGATTTCAAAAACGATAG / ACAATATTTTGATCCCTCCA / 52.3 / 53 / 171 / 165, 180, 190 / 3
57 / contig10096 / Giloe SSR57 / TCAAGCAACAAAAACAAAAA / AACCTAAATGTAGCAAAAGAGG / 48.2 / 53 / 152 / 165, 175 / 2
58 / Tinospora-est-c13290 / Giloe SSR58** / TTTATCATACGAAGCCTTGC / AAGCTTCGCAAAATAAGAAT / 54.3 / 53 / 198 / 145, 185 / 2
59 / contig07008 / Giloe SSR59 / GGAAAAGGGCTTTAAGCAT / GGCCTCCACTTAAAATGATA / 53 / 53 / 172 / 170, 185 / 2
60 / contig01051 / Giloe SSR60** / AGTGCAAGAATAGGGAGCTT / GTAACCTTCTCACCCACTCA / 56.4 / 53 / 152 / 155, 205 / 2
61 / contig01714 / Giloe SSR61 / AAATCTCGCTCCATATTTCA / GTGATGATCGGTGAGTCTTC / 52.3 / 53 / 156 / 145, 170 / 2
62 / contig02153 / Giloe SSR62 / TCATAAAGGAAGAGTTGGTGA / CCAGGAAGTTCTGTTGAGAG / 55.5 / 53 / 151 / 155, 170 / 2
63 / contig01357 / Giloe SSR63 / ATCTTTTTGCCAGAGATCAA / TCGTCTCTTCGCTTTTTAAT / 52.3 / 53 / 170 / 180, 195, 205 / 3
64 / contig13264 / Giloe SSR64** / TCAACTTTGGTCTTGTCTCC / CCATTTAGAGCAACAAAAGC / 56.4 / 53 / 158 / 175 / 1
65 / contig01592 / Giloe SSR65** / AGCGACTCGTACAGAAAAAG / AAGTACTTAGCCGCATGTTC / 56.4 / 53 / 153 / 160, 180 / 2
66 / contig04344 / Giloe SSR66 / GCTTGTGTGTGATCTGAAAA / AATCGAAAAACCTCCAAATC / 54.3 / 53 / 150 / 155, 180 / 2
67 / contig03742 / Giloe SSR67 / TGAAATAATCATGTTGAGTTGG / TAAAAACAATTAGCTTCGCTCT / 54.7 / 53 / 150 / 150 / 1
68 / contig03528 / Giloe SSR68 / CATCTTCCCATTTTCTTTTG / GCTTGGAAAGTCCTAACAGA / 52.3 / 53 / 154 / 145, 175 / 2
69 / Tinospora-est-c4780 / Giloe SSR69** / TCTCCAACCAACAAATTACA / TAGGTGAGGTGGATGGATAA / 52.3 / 53 / 159 / 175 / 1
70 / contig00334 / Giloe SSR70 / CGAATTATGTCCCCTTTTAC / TGAAAATCAGAAATTTTATCCA / 54.3 / 53 / 150 / 155, 160 / 2
71 / contig04344 / Giloe SSR71** / AGCAAGAATTCAGCTTGTGT / GCATAGATTGTAGCAACGTG / 54.3 / 51.4 / 159 / 175, 260 / 2
72 / contig01676 / Giloe SSR72 / CATCAACACCAAATTCATTG / TCGAGGCGAAGTAAACTG / 52.3 / 50.9 / 153 / 105, 195 / 2
73 / contig02939 / Giloe SSR73 / TTCATAGTCCAATGTTGTGC / TTTCTGCTTTCTGTCTCCTG / 54.3 / 51.4 / 152 / 160, 170 / 2
74 / contig05451 / Giloe SSR74** / GAATCTCCTTCTCCATTCCT / AACTCCAAGCAAAGTTCATC / 56.4 / 53 / 151 / 220 / 1
75 / contig05692 / Giloe SSR75 / TATATCCCTCCCTCCTCTTC / GTGGGACGTTAGAAAAATTG / 58.4 / 51.4 / 177 / 1,75,180 / 2
76 / contig08575 / Giloe SSR76 / TCATAAATGTGGAGCAAACC / TTTTATTGGCACTTGAGGAT / 54.3 / 53 / 150 / 145, 160 / 2
77 / contig11835 / Giloe SSR77 / AACTAGACTTCCAACCATTCC / GCAGCAACAATGAGTGAATA / 57.4 / 53 / 150 / 170, 175 / 2
78 / contig09898 / Giloe SSR78 / CACTGAACTTGCAAAAAGGT / GATTCAAGAAGCCTTTTCCT / 54.3 / 51.9 / 156 / 150, 165 / 2
79 / Tinospora-est-c12619 / Giloe SSR79 / ACTGAATCGCTTTATCGAAG / AGATCACCGCCTCAGACT / 54.3 / 52.9 / 156 / 160, 175 / 2
80 / Tinospora-est-c3083 / Giloe SSR80 / CCAAGTGATGAGCAATATCA / TTAGCAAGAGAATTGGAACC / 54.3 / 52.9 / 164 / 175, 180 / 2
81 / contig10717 / Giloe SSR81 / CCATGATTGATGATCCTCTC / GGAGAAAATTGTGTGGATTC / 56.4 / 50.9 / 154 / 170, 175 / 2
82 / Tinospora-est-c15904 / Giloe SSR82 / TCCAACTCATGTGATAATGG / AGATGCTTGGTTGAAAGAAA / 54.3 / 50.9 / 179 / 185, 210, 230 / 3
83 / contig06280 / Giloe SSR83 / GTTGAAGCAAAACCCTTTAG / ACATAAGCAAATTCGGTGAA / 54.3 / 51.9 / 156 / 160, 175, 180 / 3
84 / contig11865 / Giloe SSR84 / TCGTATCATTTTTATCAGATGAG / TTGAAGATGCCTTCAATTTT / 55.7 / 50.9 / 155 / 175, 215 / 2
85 / Tinospora-est-c14753 / Giloe SSR85 / TTCAAAACACCCATATTTGA / TGGTTGATTGAAGGAAAAAT / 50.2 / 53 / 162 / 160, 165, 170, 200 / 4
86 / Tinospora-est-c19210 / Giloe SSR86** / ACAGAAAAACACAGAAACCAA / AGGGGAAATTACAGAAGACG / 58.7 / 54 / 155 / 170 / 1
87 / contig00518 / Giloe SSR87 / GAATAGAACCCACACCAAAA / CAATGCAAAAGTTCAAGTTC / 58.9 / 54 / 151 / 140, 160, 205 / 3
88 / contig00532 / Giloe SSR88** / TCTTCAAATCTCGTCTCCAC / TACGTCCCGAAATACTCATT / 59.4 / 53.5 / 150 / 165 / 1
89 / contig00601 / Giloe SSR89** / AAAAGAAGGATGCAGTTGTG / CCCATCTGTGAAGTTTTGTT / 59.4 / 53.5 / 150 / 145, 155 / 2
90 / contig00609 / Giloe SSR90** / TCCAACACAACATAGCAGAG / GTAAATGCGTGCTTGGTAAC / 58.9 / 53.5 / 150 / 160, 175 / 2
91 / contig00584 / Giloe SSR91 / CGCACACACACACACATATAC / AGTGAAGGTTTGTGATCCAT / 60 / 54 / 157 / 170, 240 / 2
92 / contig00611 / Giloe SSR92** / ACAACCATTATCAGCATTCC / ATTAACATCACCCGTTCCTT / 58.9 / 53.5 / 160 / 175 / 1
93 / contig01410 / Giloe SSR93** / ATTTCAGGGGAGGATTACAT / GCTTCTTCACAGACCTTACG / 58.7 / 53.5 / 156 / 175 / 1
94 / contig04401 / Giloe SSR94 / GACCTAGACGATGACTTGGA / CACCAGGCACATCATATACA / 59.3 / 53.5 / 155 / 165, 200, 210, 315 / 4
95 / contig02394 / Giloe SSR95** / AGCTCACCCCATTTTTATTT / ATATGATACATGGCCCTCTG / 59 / 53.5 / 181 / 190 / 1
96 / contig08613 / Giloe SSR96 / GGGAACAAAGAATCATGAAG / AGCCGACTGAGTTGAATTT / 58.6 / 53.5 / 152 / 160, 250 / 2
Total / 182

Giloe SSR**- Monomorphic primers