Mende et al.

Tables (supplementary)

Suppl. Table 1: Primer sequences

Primer / Sequence 5´-3´
β-actin[51] / CTA GAC TTC GAG CAG GAG ATG GC
GAA TGT AGT TTC ATG GAT GCC AC
Hypoxanthine phosphoribosyltransferase (HPRT) [51] / TCC TCC TCA GAC CGC TTT T
CCT GGT TCA TCA TCG CTA ATC
Angiotensin II - receptor type 1 (AT1R) [51] / TGA AGC CAG TAC CAG CTC TGC
GCT GTG AGT TAT CCA GAC AAA ATG
Endothelin-1 (ET-1) [51] / TCC CGT GAT CTT CTC TCT GC
AGT TCG GCT CCC AAG ACA G
Transforming growth factor beta (TGF-β) [51] / TGG AGC AAC ATG TGG AAC TC
GTC AGC AGC CGG TTA CCA
Procollagen α1(I) [51] / CAT GTT CAG CTT TGT GGA CCT
GCA GCT GAC TTC AGG GAT GT
Matrix metalloproteinase 3 (MMP-3) [52] / TGG AGC TTC TGC AGA AAT A
ATC TTT TGC AAG GCC ATA G
Matrix metalloproteinase 13 (MMP-13) [52] / TAA GGC AGA AAT ATG TAG AA
AAA GCA GAG AGG GAT TAA CAA
Tissue inhibitor of matrix metalloproteinase 1 (TIMP-1) [52] / TTC CAG TAA GGC CTG TAG C
TTA TGA CCA GGT CCG AGT T


Suppl. Table 2: Grading of inflammatory disease activity in chronic hepatitis [28]

Grading Terminology / Criteria
Semiquantitive / Portal inflammation / Interface hepatitis / Lobular inflammation and necrosis
0 / None / None / None
1 / Minimal / Minimal, focal / Minimal, occasional spotty necrosis
2 / Mild / Mild, patchy / Mild, little hepatocellular damage
3 / Moderate / Moderate / Moderate, with noticeable hepatocellular change
4 / Severe / Severe, may have bridging necrosis / Severe, with prominent diffuse hepatocellular damage


Suppl. Table 3: Staging of liver fibrosis [29]

Centrilobular vein (CLV) / Normal vein or absence of vein (cirrhosis) / 0
Moderately thickened (stellate aspect of vein wall) / 1
Markedly thickened (annular aspect of vein wall with numerous fibrous extensions between hepatocytes) / 2
Perisinusoidal space (PS) / Normal / 0
Localized fibrosis / 1
Diffuse fibrosis / 2
Portal tract (PT) / Normal / 0
Enlarged without septa / 1
Enlarged with septa / 2
Cirrhosis / 3
Number of septa (NS) / Absence / 0
≤ 6 septa / 10 mm / 1
> 6 septa / 10 mm / 2
Nodular organization / 3
Width of septa (WS) / Thin and/or incomplete / 1
Thick and loose connective matrix / 2
Very thick and dense connective matrix / 3
> 2/3 biopsy area / 5
Score expression: SSS = CLV + PS + PT + 2(WS x NS)


Suppl. Table 4: Semi-quantitative analysis of bile duct proliferation [30]

Semiquantitative / Descriptive / Criteria
0 / Absence of any bile duct proliferation / 0% of portal tracts
1 / Focal bile duct proliferation / < 10% of portal tracts
2 / Multifocal bile duct Proliferation / 10-50% of portal tracts
3 / Widespread bile duct proliferation / >50% of portal tracts