Ryan H. Takahashi, Thomas A. Grigliatti, Ronald E. Reid and K. Wayne Riggs

The Effect of Allelic Variation in Aldo-Keto Reductase 1C2 on the In Vitro Metabolism of Dihydrotestosterone

The Journal of Pharmacology and Experimental Therapeutics

Supplemental Data

Table PCR primers used for site-directed mutagenesis to create AKR1C2 variant alleles. Bold highlighting in primer sequences shows single nucleotide polymorphisms introduced by site-directed mutagenesis.

Amino acid variation / NCBI Identifier (rs number) / Primer / Primer sequence
V38A / rs3207901 / forward / GTA AAG CTC TAG AGG CCG CCA AAT TGG CAA TAG AAG
reverse / CTT CTA TTG CCA ATT TGG CGG CCT CTA GAG CTT TAC
V38I / rs3207898 / forward / GTA AAG CTC TAG AGG CCA TCA AAT TGG CAA TAG AAG
reverse / CTT CTA TTG CCA ATT TGA TGG CCT CTA GAG CTT TAC
F46Y / rs2854482 / forward / CAA TAG AAG CCG GGT ACC ACC ATA TTG ATT C
reverse / GAA TCA ATA TGG TGG TAC CCG GCT TCT ATT G
H47R / rs3207905 / forward / CAA TAG AAG CCG GGT TCC GCC ATA TTG ATT CTG CAC
reverse / GTG CAG AAT CAA TAT GGC GGA ACC CGG CTT CTA TTG
S87C / rs13933 / forward / CAC TTC AAA GCT TTG GTG CAA TTC CCA TCG AC
reverse / GTC GAT GGG AAT TGC ACC AAA GCT TTG AAG TG
V111A / rs2854486 / forward / CTT CAA TTG GAC TAT GCT GAC CTC TAT CTT ATT C
reverse / GAA TAA GAT AGA GGT CAG CAT AGT CCA ATT GAA G
H170R / rs10618 / forward / GTG TCC AAC TTC AAC CGC AGG CTG CTG GAG ATG
reverse / CAT CTC CAG CAG CCT GCG GTT GAA GTT GGA CAC
L172Q / rs11474 / forward / CAA CTT CAA CCA CAG GCA GCT GGA GAT GAT CCT C
reverse / GAG GAT CAT CTC CAG CTG CCT GTG GTT GAA GTT G
K179E / rs2518042 / forward / GAT GAT CCT CAA CGA GCC AGG GCT CAA G
reverse / CTT GAG CCC TGG CTC GTT GAG GAT CAT C
K185E / rs2518043 / forward / CAG GGC TCA AGT ACG AGC CTG TCT GCA AC
reverse / GTT GCA GAC AGG CTC GTA CTT GAG CCC TG
R258C / rs28943580 / forward / CTG ATT GCC CTG TGC TAC CAG CTG C
reverse / GCA GCT GGT AGC ACA GGG CAA TCA G
Y55F / forward / GAT TCT GCA CAT GTT TTC AAT AAT GAG GAG CAG G
reverse / CCT GCT CCT CAT TAT TGA AAA CAT GTG CAG AAT C