rate( cm/kyr)
U1365 / 23°51'S / 165°39'W / 5697 / 0.031
U1366 / 26°03'S / 156°54'W / 5127 / 0.017
U1367 / 26°29'S / 137°56'W / 4285 / 0.028
U1368 / 27°55'S / 123°10'W / 3738 / 0.111
U1369 / 39°19'S / 139°48'W / 5283 / 0.037
U1370 / 41°51'S / 153°06'W / 5076 / 0.089
U1371 / 45°58'S / 163°11'W / 5306 / 0.178
Table S1 Information of sampling sites for the bottomseawater of the South Pacific Gyre (D’Hondt S, 2009)
Table S2Plate screening for extracellular enzyme activities by bacterial isolates (H/C)
Isolate(No) / AMY / CHI / CEL / T20 / T40 / T80 / DNA / LEC / CAS / GEL
A / B / C / A / B / C / A / B / C / A / B / C / A / B / C / A / B / C / A / B / C / A / B / C / A / B / C / A / B / C
SW047 / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / +++ / - / -
SW052 / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / +++ / - / -
SW084 / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / +++ / - / -
SW128 / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / W / - / - / + / + / + / - / - / -
SW129 / - / - / + / - / + / + / - / - / W / - / - / - / - / - / - / - / - / - / - / - / -
SW141 / +++ / +++ / + / - / - / - / - / - / - / + / + / ++ / + / + / ++ / - / + / + / - / - / - / - / - / - / + / W / W / + / + / +
SW142 / +++ / ++ / +++ / - / - / - / - / - / - / - / - / - / - / - / + / - / W / W / - / - / - / - / - / - / - / - / - / +++ / + / W
SW202 / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / +++ / - / -
SW204 / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / +++ / - / -
SW059 / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / +++ / ++ / ++
SW109 / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / ++ / ++ / - / - / - / - / + / - / - / - / + / -
SW079 / ++ / - / - / - / W / - / - / - / - / ++ / W / + / + / - / W / + / - / - / ++ / ++ / - / + / ++ / - / +++ / + / - / +++ / +++ / ++
SW050 / - / - / - / - / - / + / - / - / - / + / - / + / - / - / + / + / + / + / - / - / - / - / - / - / + / ++ / ++ / +++ / ++++ / +++
SW055 / - / - / - / - / - / - / - / - / - / - / + / ++ / - / - / W / - / - / ++ / - / - / - / - / - / - / + / ++ / ++ / +++ / ++++ / ++
SW127 / - / - / - / - / - / - / - / - / - / + / + / ++ / + / + / + / - / + / + / - / - / - / - / - / - / - / + / + / - / +++ / +++
SW131 / - / - / - / - / - / - / - / - / - / + / + / ++ / + / + / + / + / + / + / W / - / - / - / - / - / ++ / ++ / +++ / ++ / ++ / ++
SW135 / + / - / - / - / - / - / - / - / - / + / + / ++ / - / W / + / - / + / + / - / - / - / - / - / - / + / W / W / ++ / + / +
SW136 / - / - / - / - / - / + / - / - / - / + / ++ / +++ / + / + / + / + / ++ / + / W / W / - / - / - / - / ++ / ++ / ++ / ++ / ++ / ++
SW139 / + / W / - / - / - / - / - / - / - / + / + / + / - / + / W / + / - / W / - / - / - / W / - / - / - / + / W / ++ / + / +
SW140 / - / - / - / - / - / + / - / - / - / + / + / ++ / - / + / + / + / + / + / W / - / - / - / - / - / ++ / + / + / +++ / ++ / ++
SW143 / - / - / - / - / - / + / - / - / - / ++ / - / - / + / + / + / - / + / + / + / W / - / - / - / - / ++ / + / + / ++ / ++ / ++
SW145 / + / W / - / - / - / - / - / - / - / + / - / ++ / - / - / W / - / W / + / - / - / - / - / - / - / + / + / W / ++ / + / +
SW200 / - / W / - / - / - / - / - / - / - / W / + / + / + / - / W / W / + / + / - / - / - / - / - / - / + / + / - / +++ / + / +
SW213 / - / - / - / - / - / ++ / - / - / - / + / + / ++ / - / - / - / + / - / W / - / - / - / - / - / W / + / + / W / ++ / + / +
SW234 / - / - / - / - / - / - / - / - / - / + / + / + / - / - / W / - / W / + / W / - / - / - / - / - / - / ++ / + / - / ++ / +
SW249 / - / - / - / - / - / W / - / - / - / + / + / ++ / + / + / + / + / + / + / - / - / - / - / - / - / ++ / ++ / + / +++ / +++ / +
SW060 / - / - / - / - / - / - / - / - / - / + / ++ / ++ / + / - / + / + / ++ / + / - / + / - / - / + / + / - / - / - / +++ / ++++ / +++
SW069 / - / - / - / - / - / - / - / - / - / - / + / ++ / + / ++ / W / + / ++ / + / + / - / - / - / + / - / + / - / - / +++ / ++++ / +
SW123 / - / - / - / - / - / - / - / - / - / + / + / + / - / - / - / - / - / - / - / - / - / - / - / - / + / - / - / +++ / + / W
SW130 / - / - / - / - / - / - / - / - / - / + / + / + / + / + / W / ++ / ++ / + / W / - / - / W / W / - / W / - / - / +++ / + / +
SW121 / - / - / - / - / - / + / - / - / - / ++ / ++ / ++ / W / ++ / + / + / + / + / - / - / - / - / - / - / ++ / W / + / +++ / ++ / ++
SW124 / - / - / - / - / - / + / - / - / - / + / ++ / ++ / + / + / + / W / + / + / W / - / - / - / - / - / ++ / + / + / +++ / ++ / ++
SW206 / - / - / - / - / - / - / - / - / - / - / + / ++ / - / - / + / + / + / + / - / W / - / - / - / - / + / + / + / + / ++ / +
SW211 / - / - / - / - / - / - / - / - / - / + / + / + / - / - / + / - / W / + / - / - / - / - / - / - / W / + / - / ++ / ++ / +
SW215 / - / - / - / - / - / ++ / - / - / - / + / + / ++ / + / - / - / + / + / + / - / - / - / + / W / - / ++ / + / + / +++ / + / +
SW216 / - / - / - / - / - / - / - / - / - / + / - / ++ / - / W / W / - / W / + / - / - / - / - / - / - / - / + / + / - / ++ / ++
SW252 / - / - / - / - / - / - / - / - / - / ++ / + / ++ / - / W / + / - / + / + / - / - / - / - / - / - / - / + / + / ++ / ++ / -
SW137 / +++ / ++ / + / - / - / - / - / - / - / + / + / +++ / + / ++ / ++ / + / + / + / W / - / - / - / - / - / + / + / - / + / + / +
SW147 / +++ / ++ / ++ / - / - / - / - / - / - / + / - / ++ / + / + / ++ / - / W / + / - / - / - / - / - / - / W / - / W / + / - / +
SW146 / - / - / - / - / - / W / - / - / - / ++ / - / ++ / + / + / + / + / + / + / W / - / - / - / - / - / - / ++ / +++ / ++ / ++ / +++
SW010 / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / ++ / + / -
SW203 / - / - / - / - / - / - / - / - / - / W / + / + / + / - / - / - / - / - / - / - / - / - / - / - / + / + / W / + / + / +
SW041 / W / - / - / - / - / - / - / - / - / + / W / ++ / - / - / + / + / + / + / - / - / - / - / - / - / + / + / - / +++ / +++ / ++
SW046 / W / - / - / - / - / - / - / - / - / - / + / ++ / + / W / + / + / - / - / - / - / - / - / - / - / ++ / - / - / +++ / +++ / ++
SW051 / - / - / - / - / - / - / - / - / - / - / + / ++ / + / - / + / W / ++ / + / - / - / - / - / - / - / + / - / - / +++ / ++++ / ++
SW056 / W / - / - / - / - / - / - / - / - / + / W / ++ / + / - / + / + / + / + / - / - / - / - / - / - / ++ / + / - / +++ / +++ / ++
SW061 / W / - / - / - / - / - / - / - / - / + / W / ++ / + / - / + / + / + / W / - / - / - / - / - / - / + / W / - / +++ / + / -
SW070 / W / - / - / - / - / - / - / - / - / - / - / ++ / - / - / + / + / + / + / - / + / - / - / - / - / ++ / - / - / +++ / ++ / +++
SW201 / + / - / - / - / - / - / - / - / - / - / + / + / - / + / + / W / + / ++ / - / - / - / - / - / - / + / + / W / +++ / ++ / +
SW244 / + / - / - / - / - / - / - / - / - / ++ / + / + / - / W / + / - / + / ++ / - / - / - / - / - / - / ++ / + / + / +++ / ++ / +
SW255 / - / - / - / - / - / - / - / - / - / + / - / + / W / + / + / + / - / + / - / - / - / - / - / - / ++ / W / - / +++ / ++ / +
SW045 / ++ / ++ / ++++ / - / - / - / - / - / - / - / + / + / + / ++ / +++ / - / + / ++ / + / ++ / W / - / - / ++ / W / - / + / +++ / ++ / W
SW048 / ++ / ++ / ++++ / - / - / - / - / - / - / + / + / + / - / ++ / ++ / - / + / +++ / + / ++ / + / - / - / - / + / W / - / ++ / ++ / W
SW212 / - / +++ / +++ / - / - / - / - / - / - / - / ++ / + / + / W / ++ / W / W / + / - / + / + / - / - / - / + / - / - / +++ / + / +
SW217 / ++++ / ++ / +++ / - / - / - / - / - / - / + / ++ / + / + / - / ++ / - / + / - / + / + / - / - / - / - / - / - / - / ++ / + / +
SW218 / ++++ / ++ / +++ / - / - / - / - / - / - / + / + / + / W / W / ++ / - / + / - / - / + / W / - / - / - / W / - / - / +++ / + / +
SW219 / ++++ / ++++ / ++++ / + / W / - / - / - / - / + / + / ++ / + / ++ / ++ / W / W / + / + / W / W / - / - / - / - / - / - / ++ / + / W
SW068 / ++ / + / + / - / - / - / - / - / - / - / + / ++ / - / - / - / + / ++ / + / + / + / W / - / - / - / W / - / - / +++ / ++ / ++
SW085 / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / -
SW091 / ++ / + / + / - / - / - / - / - / - / - / + / +++ / - / - / W / - / - / + / - / - / W / - / - / - / - / - / - / +++ / ++ / ++
SW039 / ++ / ++ / +++ / - / - / - / - / - / - / - / + / + / - / + / +++ / + / + / + / + / + / + / - / - / W / - / - / - / +++ / +++ / +
SW043 / ++ / + / +++ / - / - / - / - / - / - / - / + / ++ / - / ++ / +++ / - / + / ++ / - / - / + / - / - / W / + / - / - / ++ / ++ / +
SW078 / ++ / + / ++ / - / - / - / - / - / - / + / + / ++ / - / + / + / + / + / + / ++ / ++++ / + / - / - / - / W / W / - / - / ++++ / +
SW001-1 / - / - / - / - / - / - / - / - / - / + / - / ++ / + / ++ / + / + / W / - / - / - / - / + / + / - / - / - / - / - / - / -
SW011 / - / - / - / - / - / - / - / - / - / + / + / + / + / ++ / + / ++ / + / - / - / - / - / - / + / - / - / - / - / - / - / -
SW226 / - / - / - / - / - / - / - / - / - / - / - / W / - / - / W / W / W / - / - / - / - / +++ / + / + / - / - / - / - / - / -
SW232 / - / - / - / - / - / - / - / - / - / + / + / + / + / - / - / + / - / - / - / - / - / - / + / + / - / - / - / - / - / -
SW235 / - / - / - / - / - / - / - / - / - / + / W / W / - / - / W / - / W / - / - / - / - / +++ / + / + / - / - / - / - / - / -
SW243 / - / - / - / - / - / - / - / - / - / W / - / + / - / - / - / - / - / - / - / - / - / +++ / + / + / - / - / - / - / - / -
SW250 / - / - / - / - / - / - / - / - / - / + / - / + / + / W / - / + / W / W / - / - / - / - / - / - / - / + / + / - / - / -
SW253 / - / - / - / - / - / - / - / - / - / + / - / + / + / - / - / - / - / - / - / - / - / +++ / + / + / - / - / - / - / - / -
SW074 / - / - / - / - / - / - / - / - / - / + / + / ++ / + / + / + / + / + / - / - / - / - / +++ / +++ / - / - / - / - / - / - / -
SW001 / - / - / - / - / - / - / - / W / - / - / W / + / + / + / + / + / + / + / - / - / - / - / W / - / W / - / - / - / - / -
SW008 / - / - / - / + / ++ / ++ / - / - / - / + / W / - / - / - / - / - / - / - / ++ / +++ / + / - / W / - / - / - / - / ++ / ++ / -
SW118 / - / ++ / + / + / - / - / - / - / - / - / - / W / - / - / - / - / - / - / - / ++ / ++ / - / - / - / - / - / - / - / - / -
SW108 / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / + / - / - / + / W / W
SW092 / - / - / + / - / - / - / - / - / - / - / + / + / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / -
SW110 / - / ++ / + / - / - / - / - / - / - / W / - / + / - / - / - / - / + / - / - / - / - / - / + / - / - / + / - / - / - / -
SW110-1 / - / - / + / - / - / - / - / - / - / - / + / + / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / -
SW111 / - / - / + / - / - / - / - / - / - / - / W / + / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / W
SW112 / - / - / + / - / - / - / - / - / - / - / W / ++ / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / -
SW122 / - / - / + / - / - / - / - / - / - / - / + / + / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / W
SW125 / - / - / - / - / - / ++ / - / - / - / + / + / + / - / - / - / - / W / W / - / - / - / W / W / - / - / W / + / - / ++ / +
SW134 / - / - / + / - / - / - / - / - / - / - / + / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / -
SW138 / - / - / + / - / - / - / - / - / - / - / W / + / - / - / - / - / - / - / - / - / + / - / - / - / - / - / - / - / - / -
SW075 / +++ / ++ / ++ / + / W / - / - / - / - / - / - / + / - / - / - / - / + / - / + / + / - / - / - / - / W / - / - / +++ / + / -
SW080 / - / ++ / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / +++ / - / - / - / - / - / - / - / - / -
SW082 / - / ++ / ++ / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / ++ / - / - / - / - / - / - / - / - / +
SW087 / + / + / + / - / - / - / - / - / - / + / + / ++ / + / + / + / + / + / W / - / - / - / - / + / - / - / - / - / - / - / -
SW199 / ++ / ++ / + / + / + / ++ / - / - / - / - / - / + / - / + / - / + / + / - / + / ++ / W / - / + / W / + / + / - / +++ / ++ / +
SW231 / +++ / + / ++ / + / + / - / - / - / - / + / - / + / - / - / - / + / + / - / - / - / - / - / - / - / W / - / - / +++ / + / -
SW248 / + / - / + / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / W / - / - / + / W / - / W / - / - / +++ / ++ / +
SW251 / ++ / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / + / - / - / - / - / - / +++ / ++ / +
SW228 / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / + / + / - / - / - / - / W / - / - / +++ / ++ / W
SW238 / ++ / W / - / - / W / - / + / - / - / W / - / - / - / - / - / +++ / +++ / - / + / - / - / +++ / +++ / +
SW247 / - / - / - / - / - / - / + / ++ / +++ / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / ++ / - / - / +++ / ++ / W
SW240 / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / + / - / - / - / - / - / - / - / - / -
SW246 / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / ++ / W / - / - / - / - / - / - / - / + / - / -
SW083 / - / - / - / W / - / - / - / - / - / - / - / - / - / - / + / - / - / + / + / + / - / - / + / - / - / - / - / +++ / +++ / +
SW002 / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / W / + / - / - / - / - / - / - / - / +++ / ++ / W
SW227 / - / - / - / - / - / - / - / - / - / - / - / + / - / - / W / - / - / - / + / + / W / - / - / - / - / - / - / +++ / - / -
SW089 / ++ / +++ / ++ / - / - / - / - / - / - / + / + / + / + / - / - / - / - / - / - / - / - / - / - / - / - / - / - / ++ / W / -
SW233 / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / +++ / - / -
XH117 / W / - / ++ / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / -
XH125 / - / - / - / - / - / - / - / - / - / + / ++ / ++ / + / + / +++ / - / - / - / - / - / - / - / - / - / - / - / - / +++ / +++ / ++
XH149 / + / - / ++++ / - / - / - / - / - / - / +++ / +++ / ++ / +++ / + / + / ++ / ++ / ++ / - / - / - / - / - / - / +++ / +++ / ++++ / +++ / ++++ / ++++
XH127 / - / - / - / - / - / - / - / - / - / - / ++ / ++ / + / + / ++ / ++ / - / ++ / - / - / - / - / - / - / - / - / - / ++ / ++ / +
XH147 / - / - / - / - / - / - / - / - / - / + / ++ / + / + / + / ++ / - / - / - / - / - / - / - / - / - / - / - / - / ++ / +++ / +
XH126 / - / - / - / - / - / - / - / - / - / ++ / + / ++ / + / + / ++ / - / - / - / - / - / - / - / - / - / - / - / - / ++ / ++ / ++
XH123 / - / - / - / - / ++ / ++ / - / - / - / ++ / ++ / ++ / + / + / +++ / ++ / + / + / - / - / - / - / - / - / - / - / - / +++ / +++ / ++
XH142 / - / - / - / - / ++ / ++ / - / - / - / + / ++ / ++ / + / ++ / ++ / +++ / + / + / - / - / ++ / - / - / - / ++ / - / - / +++ / +++ / +
XH124 / - / - / - / - / - / - / - / - / - / ++ / + / ++ / + / + / ++ / - / - / - / - / - / - / - / - / - / - / - / - / + / + / +
XH148 / - / - / - / - / - / - / - / - / - / ++ / ++ / ++ / + / + / ++ / ++ / + / +++ / - / - / - / - / - / - / - / - / - / +++ / +++ / ++
XH144 / - / - / - / - / - / - / - / - / - / + / + / + / + / + / ++ / - / - / - / - / - / - / - / - / - / - / - / - / +++ / +++ / ++
XH128 / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / + / ++ / +
XH115 / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / +++ / W / - / - / - / - / - / - / -
XH118 / - / ++ / +++ / - / - / - / - / - / - / + / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / +++ / ++ / ++
XH119 / - / - / - / - / - / - / - / - / - / + / + / + / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / ++ / +++ / ++
SW071 / ++ / ++ / ++++ / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / -
SW242 / ++ / + / ++ / - / - / - / - / - / - / ++ / + / ++ / + / ++ / + / - / - / - / - / - / - / + / ++ / + / - / - / - / +++ / - / -
SW072 / - / - / - / - / - / - / - / - / - / + / + / ++ / - / - / - / + / ++ / + / - / - / - / - / - / - / - / - / - / - / - / -
SW073 / - / - / - / - / - / - / - / - / - / ++ / ++ / +++ / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / -
SW113 / +++ / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / ++ / + / ++
SW132 / - / - / - / - / - / ++ / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / ++ / + / + / +++ / + / +
SW088 / ++ / ++ / ++ / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / -
SW229 / ++ / ++ / +++ / - / - / - / - / - / ++ / - / - / ++ / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / +++ / - / -
SW254 / ++ / + / ++ / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / -
SW054 / - / - / - / - / - / - / - / - / - / - / ++ / ++++ / - / + / ++ / W / - / ++ / - / - / - / - / - / - / - / - / - / - / - / -
SW057 / - / - / - / - / - / - / - / - / - / ++ / ++ / ++++ / + / ++ / ++ / - / - / - / - / - / - / - / - / - / - / - / - / - / - / -
SW245 / - / - / - / - / - / - / - / - / - / ++ / + / ++ / - / - / - / + / ++ / ++ / - / - / - / ++ / + / + / - / - / - / - / - / -
SW086 / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / ++ / + / - / - / - / - / - / - / - / - / -
SW066 / - / - / - / - / - / - / - / - / - / + / + / ++ / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / -
A:28°C; B: 16°C; C: 4°C.
AMY: amylase; CHI: chitinase; CEL:cellulose; T20: Tween20; T40: Tween40; T80: Tween80; DNA: DNase;
LEC: lecithinase; CAS: caseinase; GEL: gelatinase.
Hydrolytic circle was used in this test to detect enzymatic activities. Usually, the value of H/C is used to show them.
H: diameter of hydrolytic circle (cm);
C: diameter of colony (cm);
Size of hydrolytic circle = H/C
Weak(W): 0<H/C≤0.1; +: 0.1<H/C≤1.0; ++: 1<H/C≤2.0; +++: 2.0<H/C≤3.0; ++++: H/C>3.0;
Signs of “+”, “++” or “+++” etc was used to signify the strength of enzyme activities.
Table S3 Hydrolytic enzyme activities at 28, 16 and 4C of isolates from deep sea water
The number of strains with hydrolytic enzyme activitiesTween20 Tween40 Tween80 chitinase cellulase amylase DNase lecithinase caseinase gelatinase
The closest phylogenetic relative / Sequencesimilarity (%) / strains / 28C / 16 / 4 / 28 / 16 / 4 / 28 / 16 / 4 / 28 / 16 / 4 / 28 / 16 / 4 / 28 / 16 / 4 / 28 / 16 / 4 / 28 / 16 / 4 / 28 / 16 / 4 / 28 / 16 / 4
α-Proteobacteria
Loktanella tamlensis / 98.37 / XH117 / 1 / 0 / 0 / 1 / 0 / 0 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 0 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Seohaeicolasaemankumensis / 99.32 / SW044 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Sulfitobact
rlitoralis / 99.546-99.773 / A / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 0 / 0
Sulfitobacterpontiacus / 99.567 / SW063 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
β-Proteobacteria
Achromobacterspaiu
/ 99.093 / SW005 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Achromobacterinsolitus / 100 / SW239 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
γ-Proteobacteria
Acinetobacterjunii / 99.430-100 / B / 7 / 4 / 8 / 5 / 3 / 4 / 5 / 5 / 1 / 0 / 0 / 0 / 0 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 5 / 8 / 6 / 1 / 0 / 0 / 0 / 0 / 0
Acinetobacter johnsonii / 99.550 / SW074 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Acinetobacter haemolyticus / 99.093 / SW001 / 0 / 0 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Aestuariibacterhalophilus / 99.37 / SW062 / 1 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 0
Allomonasenterica / 100 / SW067 / 1 / 1 / 1 / 0 / 1 / 0 / 0 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 1 / 0 / 1 / 0 / 1 / 0 / 0 / 1 / 1 / 0
Alteromonas hispanica / 98.413-99.784 / C / 6 / 8 / 9 / 0 / 5 / 9 / 8 / 7 / 9 / 0 / 0 / 0 / 0 / 0 / 0 / 7 / 0 / 0 / 1 / 1 / 0 / 1 / 0 / 1 / 9 / 5 / 2 / 9 / 9 / 9
Alteromonas stellipolaris / 99.502-99.664 / D / 4 / 6 / 6 / 0 / 5 / 6 / 2 / 6 / 5 / 1 / 1 / 0 / 0 / 0 / 0 / 5 / 6 / 6 / 4 / 6 / 4 / 0 / 0 / 1 / 5 / 2 / 0 / 5 / 6 / 5
Alteromonas macleodii / 99.44-100 / E / 0 / 2 / 2 / 0 / 0 / 1 / 1 / 2 / 2 / 0 / 0 / 0 / 0 / 0 / 0 / 2 / 2 / 2 / 1 / 1 / 2 / 0 / 0 / 0 / 1 / 0 / 0 / 2 / 2 / 2
Alteromonas tagae / 99.565-99.573 / SW039,SW043 / 0 / 2 / 2 / 0 / 2 / 2 / 1 / 2 / 2 / 0 / 0 / 0 / 0 / 0 / 0 / 2 / 2 / 2 / 1 / 1 / 2 / 0 / 0 / 2 / 1 / 0 / 0 / 2 / 2 / 2
Alteromonas simiduii / 99.767 / SW078 / 1 / 1 / 1 / 0 / 1 / 1 / 1 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 1 / 1 / 1 / 1 / 0 / 0 / 0 / 1 / 1 / 0 / 1 / 1 / 1
Alteromonas fuliginea / 100 / XH144 / 1 / 1 / 1 / 1 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 1
Glaciecola mesophila / 99.119 / SW205 / 1 / 0 / 1 / 1 / 0 / 1 / 0 / 0 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 1
Glaciecola agarilytica / 99.274 / SW210 / 0 / 0 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 0 / 0
Halomonas meridiana / 99.536-100 / F / 1 / 2 / 3 / 1 / 1 / 2 / 0 / 2 / 3 / 0 / 0 / 0 / 0 / 0 / 0 / 2 / 2 / 3 / 0 / 0 / 0 / 1 / 0 / 0 / 1 / 2 / 2 / 6 / 2 / 2
Halomonas axialensis / 100 / SW007,SW058,SW059 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 1
Halomonas titanicae / 99.093 / SW109 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 0 / 0 / 0 / 0 / 1 / 0 / 0 / 0 / 1 / 0
Halomonas stevensii / 99.773 / SW079 / 1 / 1 / 1 / 1 / 0 / 1 / 0 / 0 / 0 / 0 / 1 / 0 / 0 / 0 / 0 / 1 / 0 / 0 / 1 / 1 / 0 / 1 / 1 / 0 / 1 / 1 / 0 / 1 / 1 / 1
Halomonas johnsoniae / 99.82 / SW081 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Halomonas boliviensis / 99.032 / XH143 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Idiomarina tainanensis / 98.376 / SW077 / 1 / 0 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 0
Marinobacter lipolyticus / 96.288-97.566 / SW071,SW242 / 1 / 1 / 2 / 2 / 1 / 2 / 1 / 2 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 2 / 2 / 2 / 0 / 0 / 0 / 1 / 2 / 0 / 0 / 0 / 0 / 2 / 0 / 0
Marinobacter hydrocarbonoclasticus / 99.536-99.768 / SW072,SW073 / 1 / 1 / 2 / 2 / 1 / 2 / 1 / 2 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 0 / 0
Marinobacter bryozoorum / 97.50 / SW090 / 0 / 0 / 0 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Oceanospirillum linum / 96.643-96.804 / SW004,SW053 / 0 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 0 / 0
Pseudomonas xanthomarina / 98.82-100 / G / 5 / 1 / 3 / 3 / 0 / 3 / 5 / 5 / 4 / 0 / 0 / 0 / 0 / 0 / 0 / 2 / 2 / 2 / 0 / 0 / 0 / 2 / 5 / 1 / 1 / 0 / 0 / 2 / 1 / 0
Pseudomonas pelagia / 97.118-98.043 / SW054,SW057 / 2 / 2 / 2 / 1 / 2 / 2 / 1 / 0 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Pseudomonas stutzeri / 98.32-99.778 / SW229,SW254 / 1 / 0 / 1 / 0 / 0 / 0 / 2 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 2 / 2 / 2 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 0 / 0
Pseudomonas toyotomiensis / 99.50 / SW237 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Pseudomonas moorei / 99.526 / SW008 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Pseudomonas monteilii / 100 / SW132 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 1 / 1 / 1 / 1
Pseudomonas taiwanensis / 98.89 / SW133 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Pseudoalteromonas paragorgicola / 99.80-100 / H / 15 / 13 / 16 / 9 / 10 / 14 / 10 / 12 / 16 / 1 / 0 / 8 / 0 / 0 / 0 / 4 / 3 / 1 / 11 / 3 / 0 / 3 / 3 / 1 / 16 / 16 / 14 / 12 / 16 / 15
Pseudoalteromonas mariniglutinosa / 98.701-98.918 / SW060,SW069,
SW123,SW130 / 4 / 4 / 4 / 3 / 2 / 3 / 3 / 3 / 4 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 2 / 1 / 0 / 1 / 3 / 1 / 3 / 2 / 0 / 4 / 4 / 4
Pseudoalteromonas tetraodonis / 93.30-100 / I / 7 / 8 / 9 / 5 / 6 / 8 / 5 / 7 / 9 / 0 / 0 / 3 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 0 / 0 / 1 / 1 / 7 / 7 / 6 / 9 / 9 / 8
Pseudoalteromonas marina / 99.747-99.756 / SW137,SW147 / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 0 / 0 / 0 / 0 / 0 / 0 / 2 / 2 / 2 / 1 / 0 / 0 / 0 / 0 / 0 / 2 / 2 / 1 / 2 / 1 / 2
Pseudoalteromonas aliena / 99.772-99.79 / SW146,XH126 / 2 / 1 / 2 / 2 / 2 / 2 / 1 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 2 / 2 / 2
Pseudoalteromonas espejisana / 99.78-99.78 / XH123,XH142 / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 2 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 0 / 0 / 0 / 1 / 0 / 0 / 2 / 2 / 2
Pseudoalteromonas undina / 100 / XH124 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 1 / 1 / 1 / 1
Pseudoalteromonas issachenkonii / 97.72 / XH148 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 1
Pseudoxanthomonas kalamensis / 96.380 / SW010 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 1
Pseudoalteromonas agarivorans / 100 / SW203 / 1 / 1 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 1 / 1 / 1 / 1
Psychrobacter faecalis / 99.546 / SW006 / 0 / 0 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 0 / 0 / 0 / 1 / 1 / 0
Shewanella marisflavi / 99.32-99.763 / J / 3 / 3 / 4 / 4 / 4 / 4 / 4 / 4 / 4 / 1 / 0 / 2 / 1 / 1 / 1 / 0 / 4 / 2 / 4 / 2 / 0 / 3 / 4 / 1 / 3 / 2 / 1 / 4 / 4 / 4
Shewanella aquimarina / 100 / SW066 / 1 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1
Shewanella haliotis / 97.159 / SW086 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1
Shewanella pacifica / 98.886 / SW245 / 1 / 1 / 1 / 0 / 0 / 0 / 1 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 0 / 0 / 1 / 1 / 1 / 0 / 0 / 0 / 0 / 1 / 0
Vibrio campbellii / 97.964-99.773 / K / 2 / 9 / 9 / 1 / 1 / 1 / 1 / 3 / 2 / 1 / 1 / 2 / 0 / 0 / 0 / 0 / 1 / 8 / 0 / 0 / 3 / 1 / 2 / 0 / 1 / 2 / 1 / 2 / 1 / 5
Vibrio harveyi / 98.190-99.688 / SW075,SW080,SW082 / 0 / 0 / 1 / 0 / 0 / 0 / 0 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 3 / 2 / 0 / 1 / 0 / 0 / 0 / 0 / 1 / 0 / 0 / 1 / 1 / 1
Vibrio sagamiensis / 98.364-98.46 / SW008,SW118 / 1 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 2 / 1 / 1 / 0 / 0 / 0 / 0 / 1 / 1 / 1 / 2 / 2 / 0 / 1 / 0 / 0 / 0 / 0 / 1 / 1 / 0
Vibrio diazotrophicus / 99.546 / SW065 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Vibrio vulnificus / 99.688 / SW087 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 1 / 0 / 0 / 0 / 0 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Vibrio chagasii / 100 / SW199 / 0 / 0 / 1 / 0 / 1 / 0 / 1 / 1 / 0 / 1 / 1 / 1 / 0 / 0 / 0 / 1 / 1 / 1 / 1 / 1 / 1 / 0 / 1 / 1 / 1 / 1 / 0 / 1 / 1 / 1
Vibrio inhibens / 99.59 / SW231 / 1 / 0 / 1 / 0 / 0 / 0 / 1 / 1 / 0 / 1 / 1 / 0 / 0 / 0 / 0 / 1 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 0 / 0 / 1 / 1 / 0
Vibrio splendidus / 99.83 / XH112 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Xanthomonas vesicatoria / 95.82 / SW003 / 1 / 0 / 0 / 0 / 0 / 0 / 1 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 1 / 0 / 0 / 0 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 0
Firmicutes
Bacillus anthracis / 99.779-99.779 / SW248,SW251 / 0 / 0 / 0 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 2 / 0 / 1 / 2 / 1 / 0 / 2 / 1 / 0 / 1 / 0 / 0 / 2 / 2 / 2
Bacillus oceanisediminis / 99.80 / SW228 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 0 / 0 / 0 / 0 / 1 / 0 / 0 / 1 / 1 / 1
Bacillus cereus / 99.778 / SW238 / 0 / 0 / 0 / 1 / 0 / 0 / 1 / 0 / 0 / 0 / 1 / 0 / 0 / 0 / 0 / 1 / 1 / 0 / 0 / 0 / 0 / 1 / 1 / 0 / 1 / 0 / 0 / 1 / 1 / 1
Bacillus safensis / 100 / SW247 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 0 / 0 / 1 / 1 / 1
Bacillus humi / 97.822 / XH114 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 0
Marinilactibacillus piezotolerans / 100 / SW083 / 0 / 0 / 0 / 0 / 0 / 1 / 0 / 0 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 0 / 0 / 1 / 0 / 0 / 0 / 0 / 1 / 1 / 1
Staphylococcus / 99.769-100 / L / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 0 / 0
Actinobacteria
Microbacterium / 99.186-100 / M / 0 / 0 / 1 / 0 / 0 / 1 / 0 / 0 / 0 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 3 / 2 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 4 / 2 / 2
Micrococcus antarcticus / 99.80 / XH130 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Streptomyces flocculus / 99.44 / XH115 / 1 / 0 / 0 / 1 / 1 / 0 / 1 / 0 / 0 / 0 / 0 / 0 / 1 / 0 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Bacteroidetes
Algibacter mikhailovii / 96.342 / XH116 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Aquimarina latercula / 99.819 / XH118 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 1
Aquimarina macrocephali / 96.76 / XH119 / 1 / 1 / 1 / 0 / 1 / 1 / 0 / 0 / 0 / 0 / 1 / 0 / 0 / 0 / 0 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 1
Tenacibaculum aiptasiae / 97.619 / XH113 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
Zunongwangia profunda / 100 / SW089 / 1 / 1 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 1 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 1 / 1 / 0
Totle / 174 / 86 / 86 / 112 / 56 / 60 / 81 / 69 / 79 / 77 / 10 / 10 / 19 / 6 / 5 / 6 / 44 / 41 / 44 / 47 / 36 / 22 / 25 / 42 / 18 / 65 / 47 / 31 / 104 / 97 / 87
A-M: All isolates in this species
Name of strains:
A: SW040, SW042, SW047, SW049; B: SW001-1, SW011, SW226, SW232, SW235, SW243, SW250, SW253; C: SW041, SW046, SW051, SW056, SW061, SW070, SW201, SW244, SW255;
D: SW045, SW048, SW212, SW217, SW218, SW219; E: SW068, SW085, SW091; F: SW009, SW052, SW084, SW128, SW129, SW141, SW144, SW202, SW204, XH131, SW142;
G: SW064, SW076, SW207, SW208, SW209; H: SW050, SW055, SW127, SW131, SW135, SW136, SW139, SW140, SW143, SW145, SW200, SW213, SW234, SW249, SW125, SW149;
I: SW121, SW124, SW206, SW211, SW215, SW216, SW252, XH127, XH147; J: SW119, SW129, SW126, SW166; K: SW108, SW092, SW110, SW110-1, SW111, SW112, SW122, SW125, SW134, SW138;
L: SW214, SW240, SW241, SW246; M: SW002, SW227, XH128, XH129.
Table S4 Comparison of relative activity at low temperature of Amy2235 with other organisms from different sources
Amylase / Special feature / Relative activity / Optimum temperature / ReferenceAmy2235 / 0℃, 18.7%; 10℃, 36% / 50℃ / Thisstudy
AHA / Cold active / 0℃, ~ 20%; 18℃, 13% / Around 40℃ / (FellerG et al, 1992)
LAMY / Mesophilic / 20℃, 15% / 55℃ / (Igarashi K et al, 1998)
Amy121 / Thermophilic / 70℃,<90% / 75℃ / (Li L et al, 2015)
BLA / Thermophilic / 20℃, <5% / 90℃ / (Li L et al, 2015)
References
FellerG, Lonhienne T, Deroanne C, Libioulle C, Van BJ, Gerday C (1992)Purification, characterization, and nucleotide sequence of the thermolabile alpha-amylase from the antarctic psychrotroph Alteromonas haloplanctis A23. J Biol Chem267:5217–5221
Igarashi K, Hatada Y, Ikawa K, Araki H, Ozawa T, Kobayashi T,Ozaki K, Ito S (1998) Improved thermostability of a Bacillusalpha-amylase by deletion of an arginine-glycine residue iscaused by enhanced calcium binding. Biochem Biophys ResCommun 248:372–377
LiLZ, Yang J, Li J, Long LJ, Xiao YZ, Tian XP, Wang FZ,Zhang S (2015)Role of two amino acid residues’ insertion on thermal stability of thermophilic α-amylase AMY121 from a deep sea bacterium Bacillus sp. SCSIO 15121. Bioprocess Biosyst Eng 38:871–879
Figure S1. Transparent circles or hydrolytic circles produced by bacteria on different media at 4C (Transparent circles or hydrolytic circles are indicated by black arrows.)
A The starch medium B The casein seawater medium C The seawater lecithin medium DThe seawater chitin medium E DNase agar mediumF The seawater of cellulose medium G Tween 80 medium H Tween 40 medium I Tween 20 mediumJ Gelatinase medium
Fig.S2Multiple sequence alignment of the amino acid sequences of Amy2235 with α-glucosidase of Xanthomonas sp.Mitacek01 (WP_055249184.1),Luteimonas sp. FCS-9 (WP_047136483.1), and Pseudoxanthomonas suwonensis (WP_024868235.1)
Sample7GL002235, Amy2235; Luteimonas, Luteimonas sp. FCS-9 (WP_047136483.1); Pseudoxanthomonas, Pseudoxanthomonas suwonensis (WP_024868235.1); Xanthomonas, Xanthomonas sp.Mitacek01 (WP_055249184.1)
Symbols: *, identical residues in all sequences; :, conserved substitutions; ., semiconserved substitutions; -, gaps introduced during the alignment process. Numbers on the right refer to the amino acid residue at the end of each line.
Shaded residues (black), identical residues in all sequences; Shaded residues (gray), conserved substitutions