TABLE S2. Strains, Plasmids and Oligonucleotides Used in This Study

TABLE S2. Strains, plasmids and oligonucleotides used in this study

Strains / Characteristics / Source
E. coli
EC100λpir / supE44 ΔlacU169 (80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 (λpirR6K) / Epicenter
SM10λpir / thi-1 thr leu tonA lacY supE recA::RP4-2-Tc::Mu Kmr (λpirR6K) / Lab collection
V. cholerae
JB3 / V. cholerae O1 El Tor strain N16961, SmR / Lab collection
JB58 / V. cholerae O1 El Tor strain N16961, SmRlacZ / Lab collection
HK1 / V. cholerae O1 El Tor strain HK1, SmR / (1)
MO10 / V. cholerae O139 El Tor strain MO10, SmR / (1)
XBV222 / JB58 ΔleuO / This study
JB460 / JB3 ΔtoxT / (2)
JB461 / JB3 ΔtoxRS / (2)
JB462 / JB3 ΔtcpPH / (2)
DT730 / JB460 ΔlacZ / This study
DT733 / JB461 ΔlacZ / This study
DT736 / JB462 ΔlacZ / This study
Plasmids
pTL61T / lacZ transcriptional reporter plasmid, CbR / (3)
pXB266 / pTL61T containing the leuO promoter region / This study
pBAD18Km / Arabinose regulated expression plasmid, KmR / (4)
pBAD18 / Arabinose regulated expression plasmid, CbR / (4)
pXB269 / 1120 bp leuO and VC2486 ORF cloned at EcoRI/XbaI in pBAD18Km / This study
pXB286 / toxRmemS cloned at SacI/SphI sites in pBAD18 / This study
pXB289 / toxRS cloned at SacI/SphI sites in pBAD18 / This study
pXB297 / pBAD18Km containing VC2486 ORF / This study
pXB298 / pBAD18Km containing leuO ORF / This study
pWM91ΔlacZ / pWM91 containing the franking fragments of lacZ / (5)
pWM91ΔleuO / pWM91 containing a fragment of leuO harboring an internal deletion / (6)
pCM10 / luxCDABE reporter plasmid, KmR / (7)
pJB906 / pCM10 containing the leuO promoter / This study
PCR primers
PleuO-F-SmEc / CCCCCGGGAATTCCTGACTAACTCAATGAGTCCTTCGTTAAAAC
PleuO-R-BamH / GGGGATCCGCGTCTTTTTTATCTAACATTTGCATGCCT
pBAD-toxR-F / GGGAGCTCGGTCCTCAAAAGAGATATCGATGAGTCATATTGG
pBAD-toxR-R / AACCCGGGCATGCCGCTCAGTCAGGTTAAGAATTACTGAACAG
pBAD-leuO-F / AAGAATTCGTCAATGCTATTGCATAGCTTCGAATGATTAGCCG
pBAD-leuO-R / AATCTAGACAAAGGATCAGCGATTCGCGACCACTTCGCC
pBAD-toxRmem-F1 / GGGAGCTCGGTCCTCAAAAGAGATATCGATGAGTCATATTGG
pBAD-toxRmem-F2 / CTGCTCACTAACTAGGATCTTGCTATGCAAAATAGACACATCGCC
pBAD-toxRmem-R1 / AACCCGGGCATGCCGCTCAGTCAGGTTAAGAATTACTGAACAG
pBAD-toxRmem-R2 / AGCAAGATCCTAGTTAGTGAGCAGTAATACTGCGAGGGGAAG
qPCR primers
leuO-F / GACCACTTCGCCACAAATCACCA
leuO-R / CGTTGGATGGCGGAAAATGCG
aphA-F / GCAGAACCTTACCGTCTGCAA
aphA-R / GCGTAATAAGCGGCTTCGATT
aphB-F / ATCGGTGAAGTGAAAGACATTTTGG
aphB-R / TGATGCAACTCTTCAGCATGAGTC
tcpP-F / GGTGGAGTTATGGCCAATGG
tcpP-R / GTTATCCCCGGTAACCTTGCT
toxR-F / CGTCAAAACGGTTCCGAAACG
toxR-R / CGCGAGCCATCTCTTCTTCAA
16sRNA-F / CTTTTTGAATCGCAGCAGGT
16sRNA-R / GGTGGTCGAATCATGAGCTT

References.

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