Table S1Hardy-Weinberg Equilibrium (HWE) results for nine microsatellite loci applied in six populations of Geophagus brasiliensis. Test for deviation from expected HWE proportions (P-value = 0.05) and adjustment after Sequential Bonferroni correction α/(n - i + 1), α = 0.05.*Significant deviations in the Hardy-Weinberg Equilibrium – P-value <α/(n - i + 1).

Hardy-Weinberg Equilibrium
Significant P-value after Sequential Bonferroni correction
Gbra16 / Gbra17 / Gbra21 / Gbra25 / Gbra47 / Gbra55 / Gbra62 / Gbra63 / Gbra96
S1 / 0.5032 / 0.0021* / 0.0114 / 0.5415 / 0.0354 / 0.1918 / 0.6711 / 0.1029 / 0.1673
S2 / 0.1596 / 0.0044* / 0.0447 / 1.0000 / 0.5769 / 0.0023* / 0.6988 / 0.4161 / 0.2358
S3 / 0.4867 / 0.6055 / 0.0936 / 0.1672 / 0.1017 / 0.0645 / 0.7699 / 0.6633 / 0.0985
S4 / 0.6009 / 1.0000 / 0.5813 / 1.0000 / 0.9556 / 0.9426 / 0.5834 / 0.6167 / 0.0040
S5 / 0.7513 / 0.4114 / 0.1863 / 1.0000 / 0.0903 / 0.6694 / 0.3399 / 1.0000 / 0.4036
S6 / 0.8281 / 0.1881 / 0.5418 / 0.0611 / 0.0632 / 0.7874 / 0.0063 / 0.7772 / 0.0035*
Sequential Bonferroni correction -α/(n - i + 1)
Gbra16 / Gbra17 / Gbra21 / Gbra25 / Gbra47 / Gbra55 / Gbra62 / Gbra63 / Gbra96
S1 / 0.0166 / 0.0055 / 0.0062 / 0.0250 / 0.0071 / 0.0125 / 0.0500 / 0.0083 / 0.0100
S2 / 0.0083 / 0.0062 / 0.0071 / 0.0500 / 0.0166 / 0.0055 / 0.0250 / 0.0125 / 0.0100
S3 / 0.0125 / 0.0166 / 0.0062 / 0.0100 / 0.0083 / 0.0055 / 0.0500 / 0.0250 / 0.0071
S4 / 0.0083 / 0.0250 / 0.0062 / 0.0500 / 0.0166 / 0.0125 / 0.0071 / 0.0100 / 0.0055
S5 / 0.0166 / 0.0100 / 0.0062 / 0.0250 / 0.0055 / 0.0125 / 0.0071 / 0.0500 / 0.0083
S6 / 0.0500 / 0.0100 / 0.0125 / 0.0071 / 0.0083 / 0.0250 / 0.0062 / 0.0166 / 0.0055