Table S1 The primers of V-ATPase subunits used in the current study

Gene / Primer sequence / gene / Primer sequence
At1g78900 (A) / 5’- ATGGTATGGCCGGTGCTGCT-3’ / At2g28520 (a3) / 5’- CAGATATTGCACTTGGAG-3’
5’- GGATATTCTTGCAATAGTCT-3’ / 5’- ATCTCAATCCTGATTGAT-3’
At1g76030 ( B1) / 5’-TCTGCCAGAAGCATACCTTG-3’ / At2g21410 (a1) / 5’-GGAGTACAAGCTCGTTCTTG-3’
5’-CCATGATCCTCAAGTAGATC-3’ / 5’-CTGTCGTATGAAGATGTTGC-3’
At4g38510 ( B2) / 5’- TCTGGAAGTTCTATCAATC-3’ / At4g39080 ( a2) / 5’-TGACATTGACTTGGATGATG-3’
5’- CATTGCTGCGAACACAATG-3’ / 5’-GTCGAATCAATGGACTTCTC-3’
At1g20260 (B3) / 5’-CTGTGTCATTGGACATGCTT-3’ / At4g34720 (c1) / 5’-GGAGTTATGAGACCTGAGT-3’
5’-CCACGAGCAATGGAGTTCAT-3’ / 5’-AATGGCCATTCCAGCTGAG-3’
At1g12840 (C) / 5’-CGATGATCTGCTTAAGTCG-3’ / At1g19910 (c2) / 5’-GTGTGATGAGACCAGAGCT-3’
5’-GAACCTTGAGATCATCCTC-3’ / 5’-GTTGTGCATTCGCTCTAAC-3’
At3g58730 (D) / 5’-TACAGCTAAGGAGTCTATG-3’ / At4g38920 (c3) / 5’-CTCATGTATGGGAGCTGCGT-3’
5’-CTGTTGACCACCTCTAGCT-3’ / 5’-CATCGCCAACGATACCTATG-3’
At4g11150 (E1) / 5’-GATTGATTACTCTATGCAGC-3’ / At1g75630 (c4) / 5’-GAGTGATGAGACCTGAACT-3’
5’-CATCAAGTACAGCTTCCACT-3’ / 5’-AATCAGGATTACAATCAAC-3’
At3g08560 (E2) / 5’- CTCGAGTCTGCCAAGCGT-3’ / At4g26710 (e2) / 5’-ATGAGAGGGTTCATCTGCG-3’
5’- TCTCAATGATGAGACTCT-3’ / 5’-CGACGATCCTCCATCATCC-3’
At1g64200 (E3) / 5’-AGATTGATTACTCTATGCAG-3’ / At2g16510 (c5) / 5’-GGAGTGATGAGACCTGAGT-3’
5’-CATCAAGTACAGCTTCCACT-3’ / 5’-ATACCAGCTGAGAGTCCAG-3’
At3g42050 (H) / 5’- GTTAGCATCTTACGTGATAT-3’ / At4g32530 (c''1) / 5’-AATCTCATCAGTGTCATCT-3’
5’- CCAATAACAGCATTACCAGC-3’ / 5’-AACAACCCGAGAGCACTCC-3’
At4g25950 (G3) / 5’-GACGGATTGTTTCAGCTGCG-3’ / At2g25610 (c''2) / 5’-GAATCTCATCAGTGTAATCT-3’
5’-TACTTGATGAGCATCTTGAC-3’ / 5’-AGCGTCGAGTTCTGAGCATC-3’
At3g01390 (G1) / 5’-TGAGGTAGAAGCTCAACAC-3’ / At3g28715 (d2) / 5’-ATCTATGTCAGTGTGAGAAC-3’
5’-CTTCGTTCTTCAACTGCTCG-3’ / 5’-GACCATATCTGATGTACTCT-3’
At4g02620 (F) / 5’-TCATCGCAATGATCGCCGAT-3’ / At5g55290 (e1) / 5’-ATAACAACCCTAATCTTCG-3’
5’-ATCTCCAAGATTGCAGGAAC-3’ / 5’-CTACTCTGTTTCGCTTAGA-3’
At4g23710 (G2) / 5’-ATCCAGCAACTGCTTGCTGC-3’ / At3g28710 (d1) / 5’-ATCGTTAGAGGCCACCGTG-3’
5’-CAGTTGTTCACTGTGGTCAC-3’ / 5’-AGACATTGGCTCAGTAGCT-3’

Table S2 The primers of NAC, ABF and DREB used in qRT-PCR

GeneBank No. / Gene Name / 5’-primer / 3’-primer
AT1G01010.1 / NAC001 / 5’-ACATCTGTAGCTACGATC-3’ / 5’-TATGACCAATCTTACCAC-3’
AT1G02250.1 / NAC005 / 5’-AGTAACACGAGCCATGTC-3’ / 5’-CGATCTTCTCACGATCTC-3’
AT1G32870.1 / NAC013 / 5’-GACTAGAGATCGTCAATG-3’ / 5’-TGTATACTCATGCATCAC-3’
AT1G34180.2 / NAC016 / 5’-CTACTGATGAAGAGCTTG-3’ / 5’-CTAGCTGCGTTAGGATAC-3’
AT1G34190.1 / NAC017 / 5’-TCATCCAACTGATGAAG-3’ / 5’-TGGATACTTCCTACTCCT-3’
AT1G77450.1 / NAC032 / 5’-GATGAGGAGCTAGTCCTC-3’ / 5’-ATATCCAGTACCAGCTGC-3’
AT3G04060.1 / NAC046 / 5’-AGGTCTTCAACATCCGAT-3’ / 5’-GGAGCTCTTCCTGTATAG-3’
AT3G10500.1 / NAC053 / 5’-GAGTGGTACTTCTTCAGT-3’ / 5’-CAACCGATACTCATGCAT-3’
AT3G15500.1 / NAC055 / 5’-GACTGACGAAGAGCTGAT-3’ / 5’-CTCCGTCGAGATAACTT-3’
AT5G04410.1 / NAC078 / 5’-GATGAGGAACTTGTTCGC-3’ / 5’-GTAGCACGATTCGTCTTG-3’
AT5G08790.1 / NAC081 / 5’-CGAGATTGATCTCTACAAG-3’ / 5’-GCGAGACGATACTCATGC-3’
AT5G39610.1 / NAC092 / 5’-CTCATAACTCACTACCTC-3’ / 5’-ACGATATTCATGCATAAC-3’
AT5G46590.1 / NAC096 / 5’-GAAGGTCTCGAGATTGAG-3’ / 5’-GATATTCATGCATGATCC-3’
AT5G63790.1 / NAC102 / 5’-GCAGAGATTGACTTGTAC-3’ / 5’-GCGTAGAAGACGAGTGCT-3’
AT1G49720.2 / ABF1 / 5’-TGATGAGCTTCAGAGCAC-3’ / 5’-CAGACTTCATCGACAGTC-3’
AT1G45249.3 / ABF2 / 5’-TCGATATACTCGTTGACG-3’ / 5’-TCTGACTAAGCGTTCGAG-3’
AT4G34000.1 / ABF3 / 5’-GTCTATGAACATGGATGA-3’ / 5’-AGTGAACCTTGTCTCTGC-3’
AT3G19290.3 / ABF4 / 5’-ATGGACTGCTGAGGAAGC-3’ / 5’-GAGGCACATTCGACTCAC-3’
NM_118680.1 / DREB1A / 5’-TCGTGAGACTCGTCACC-3’ / 5’-GATTCGGAGTCTCCAAGC-3’
AB013816.1 / DREB1B / 5’-TCGTGAGACTCGTCACC-3’ / 5’-AAGTTGAGACATGCTGAT-3’
NM_118679.1 / DREB1C / 5’-GCTGAGATGGCAGCTCGT-3’ / 5’-ATGAGCATCCGTCGTCAT-3’
NM_120623.2 / DREB2A / 5’-TACTACTGTGGCTGAGAG-3’ / 5’-CTGCTACCTCGATTAGG-3’
NM_111939.2 / DREB2B / 5’-AGAGTACAACGAGATTGT-3’ / 5’-ACCGTACATAGCGGTAGC-3’
NM_129594.2 / DREB2C / 5’-TGGAGAGAGTACAATGAG-3’ / 5’-GCTTCATATGAACTGGAG-3’
NM_121850.1 / DREB2G / 5’-ACTCTATGGACACGAGGC-3’ / 5’-CACTTGAAGACGAACATC-3’
AT2G38340.1 / DREB19 / 5’-AAGAGTGGTTGAGCCAGT-3’ / 5’-CACCACGGTGACTCACTG-3’
AT1G21910.1 / DREB26 / 5’-GAGCTTACGATGTTGCAC-3’ / 5’-AGACGGCTGATGAAGTAG-3’
NM_001084295.1 / DDF2 / 5’-TCAAGGAGACACGTCACC-3’ / 5’-AACCTCCAAGCAGAATCG-3’
AT2G23340.1 / DEAR3 / 5’-ATCAAGGATCTGGTTAGG-3’ / 5’-GCGCATCAACCTGAGCAC-3’
At3g18780 / Actin2 / 5’-TGCAGGAGATGATGCTCC-3’ / 5’-ATACGAAGCTCATTGTAG-3’


FigS1 Multiplesequencealignment (a) and phylogenetic tree (b) analysis the deduced amino acid sequence of JrVHAG1 protein with the VHA-G proteins from other species using the Cluster 3.0 and MEGA 4.1 (NJ method).

FigS2 GFP localization analysis of the JrVHAG1protein in onion epidermis cells. The full-length coding region of JrVHAG1 (without the stop codon) was fused with N-terminal of GFP protein to generate the fusion gene of JrVHAG1-GFP. The constructs of JrVHAG1-GFP was transformed by particle bombardment (Bio-Rad). (a), GFP fluorescence; (b),Onion peel cells imaged under bright field; (c),Merge of bright field and fluorescence. GFP-35S was used as a control


FigS3Confirmation of the JrVHAG1 transgenic plants by PCR and qRT-PCR.(a), 21 different lines of potential transgenic linesby PCR analysis. M, DL Marker2000, WT, wild type; P, postive control, PCR by the library plasmid of JrVHAG1; 1-21, 21 transgenic lines.(b), The expression of JrVHAG1 in these 21 potential transgenic lines by qRT-PCR.

Fig. S4 Biomass comparison of WT and transgenic plants under ABA treatment. a, the germination of WT, G2, G6, and G9 under ABA condition; b, the growth of WT, G2, G6, and G9 under ABA treatment; c, the germination rate according to a and figure 2a; d, primary root length according to b and figure 3a. The experiments were repeated at least three times. All data are displayed as the mean ± SD of three independent experiments, significant differences between transgenic plants and WT were marked as * (p<0.05)