Table S1. Strains and plasmids used in this work

Strain or plasmid / Description / Phenotype / Reference
Strains
E. coli
HB101 / F-Δ(gpt-proA)62 leuB6 supE44 ara-14 galK2 lacY 1 Δ (mcrC-mrr) rpsL20 (StrR) xyl-5 mtl-1recA13 / Strr / (1)

R. capsulatus

aMT1131 / crtD121Rifr / Wild Type (NADI+) / (2)
SB1003 / Wild Type (NADI+) / (3)
Y262 / GTA overproducer / (4)
B10S-T7 / Genr / (5)
SE8
SE8Ri, i=1-6 / Δ(ccoA::spe)
By-pass suppressors ofΔ(ccoA::spe) / Sper
Sper, NADI+, Cus / (6)
This work
SE8R1CuR / Δ(ccoA::spe) rev1 resistant to 100 M Cu2+ / Sper, NADI-, CuR / This work
SE8R2CuR / Δ(ccoA::spe) rev2 resistant to 100 M Cu2 / Sper, NADI-, CuR / This work
SE24 / Δ(copA::kan) / Kanr, NADI+, Cus / This work
SE25 / Δ(copA::kan) Δ(ccoA::spe) / Kanr, Sper, NADI+, Cus / This work
SE26 / Δ(RCC01042::gen) / Gmr, NADI+, CuR / This work
SE27 / Δ(RCC01042::gen) Δ(ccoA::spe) / Gmr, Sper, NADI+, CuR / This work
SE13 / Δ(ccoI::spe) Δ(ccoA::kan) / SpeR, KanR / This work
SE31 / ΔccoA ΔRCC02190 / SpeR, KanR / This work
Plasmids
pRK2013 / Conjugation helper / Kanr / (7)
pRK415 / Broad host-range vector / Tetr / (7)
pBluescript II KS+ / Cloning vector / Ampr / Stratagene
pKD4 / Δ(ccoNO::kan) / Kanr / (8)
pBS_CopA / copA cloned into the SacI and KpnI sites of pBluescript / Ampr / This work
pRK415_CopA / copA cloned into the SacI and KpnI sites of pRK415 / Tetr / This work
pBS-ΔcopA / 2.4 kb SacI-KpnI fragment carrying the Δ(copA::kan) allele on pBluescript II KS+ / Ampr ,Kanr / This work
pRK415- ΔcopA / 2.4 kb SacI-KpnI fragment carrying the Δ(copA::kan) allele on pRK415 / Tetr,Kanr / This work
pRK_CopA9C / pRK415 carrying the copA9Cframeshift allele of copA / Tetr / This work
pBS_RCC01042 / RCC01042 cloned in SacI and KpnI sites of pBluescript / Ampr / This work
pRK415_RCC01042 / RCC01042 cloned in SacI and KpnI sites of pRK415 / Tetr / This work
pBS-ΔRCC01042 / SacI-KpnI fragment carrying the Δ(RCC01042::gen) alleleon pBluescript II KS+ / Ampr ,Gmr / This work
pRK415- ΔRCC01042 / SacI-KpnI fragment carrying the Δ(RCC01042::gen) allele on pRK415 / Tetr,Gm / This work

aR.capsulatus strain MT1131 is derived from SB1003 in multiple steps, as described in Zannoniet al. (1980) (2) ; first a Ps-deficient mutant (TL1) was obtained using tetracycline suicide, then its crtD derivative was constructed by a GTA cross to yield MT113, which then was used to yield its Ps-proficient derivative via a second GTA cross.

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REFERENCES

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2.Zannoni D, Prince RC, Dutton PL, Marrs BL. 1980. Isolation and characterization of a cytochrome c2-deficient mutant of Rhodopseudomonas capsulata. FEBS Letters 113:289-293.

3.Yen HC, Marrs B. 1976. Map of genes for carotenoid and bacteriochlorophyll biosynthesis in Rhodopseudomonas capsulata. Journal of bacteriology 126:619-629.

4.Yen HC, Hu NT, Marrs BL. 1979. Characterization of the gene transfer agent made by an overproducer mutant of Rhodopseudomonas capsulata. Journal of molecular biology 131:157-168.

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8.Myllykallio H, Zannoni D, Daldal F. 1999. The membrane-attached electron carrier cytochrome cy from Rhodobacter sphaeroides is functional in respiratory but not in photosynthetic electron transfer. Proceedings of the National Academy of Sciences of the United States of America 96:4348-4353.

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