B. cereus strain / ORF name(s) / Predicted sequence specificity / ORF name(s) / Predicted sequence specificity
B. cereus 03BB102 / Bce03_0971 and Bce03_0972 / CCCGT or CCCGC (C5M) / Bce03_0978 (R1) and Bce03_0979 (R2) / GCNGC, GCSGC, or GCWGC
BCA_1903 (multi-specificity C5M) / GGCC, GCGC, CCGG, CCGC, CCNGG (C5M) / No companion R gene
B. cereus AH187 / BCAH187_A5299 / Type III Mod / BCAH187_A5300 / Type III Res
B. cereus AH603 / Bcere0026_58650 / YGGCCR (C5M) / No companion R gene
Bcere0026_8020 / GCWGC or GCSGC (C5M) / Bcere0026_8040 / GCSGC
Bcere0026_54340 / HNH endonuclease,
B. cereus AH820 / BCAH820_1008, / GCNGC (C5M)
(similar to M.BthCI) / BCAH820_1009 (R1) and BCAH820_1011 (R2) / GCNGC
BCAH820_4397 / GGCC or CCNGG (C5M) / No companion R gene
B. cereus B4264 / BCB4264_A0972 / Type III Mod / BCB4264_A0973 / Type III Res
B. cereus G9842 / BCG9842_B4338 / C5M / BCG9842_B4335 (R1) and
BCG9842_B4334 (R2) / GCNGC or GCSGC
BCG9842_B4337 / C5M
BCG9842_B5355
BCG9842_B5354 / GCNNGC or GCCGC (C5M) / BCG9842_B5356, HNH endonuclease (DHxNxK) / GCATGC (similar to SphI)
Plasmid pG9842_209 / BCG9842_0061 / Amino-MTase / No companion R gene
BCG9842_0102 / GGNCC (C5M) / BCG9842_0103 / Similar to Staphylococcal nuclease
C5M: BCG9842_0118, / TCGA (C5M) / No companion R gene
B. cereus Q1 / BCQ_1008 / GATC (similar to M.AvaVI, amino-MTase / No companion R gene
B. cereus subsp.
cytotoxis NVH 391-98 / Bcer98_0752 / GCWGC (similar to M.BbvI, (C5M)) / Bcer98_0753 / GCWGC (similar to BbvI)
Bcer98_3991 / GATC (N6mA MTase) / Bcer98_3990 / GATC (BspKT6I isosch.)
Table S1. Predicted Type II and III R and M genes in sequenced B. cereus genomesa
a. Go to REBASE/Genomes with listing of predicted R and M genes in Bacillus strains. Click “closest neighbors” to find the sequence similarity to all known R and M genes.
Table S2. Predicted R and M genes in sequenced genomes of Bacillus anthracis and Bacillus thuringiensis strainsa
Methylases (M) / Restriction (R)Bacillus strain (sp.) / ORF name(s) / Predicted sequence specificity / ORF name(s) / Predicted sequence specificity
B. anthracis A0248 / M.BanA0248ORF3836P
(prophage LambdaBa01) / YGGCCR (C5M) / No companion R
B. anthracis Ames / M.BatAORF3814P / YGGCCR (C5M) / No companion R
B. anthracis Ames Ancestor / M.BatA581ORF3814P / YGGCCR (C5M) / No companion R
B. anthracis CDC684 / M.Ban684ORF821P / YGGCCR (C5M) / No companion R
B. anthracis C1 / No predicted Type II M / No predicted Type II R
B. anthracis Sterne / M.BatSORF3531P / YGGCCR (C5M) / No companion R
B. thuringiensis Al Hakam / BthAHORF843P / Possible Type IIG N6A-methylase/endonuclease fusion (R-M-S), unknown specificity
B. thuringiensis BMB171 / M.Bth171ORF821P / GCWGC or GCSGC / BMB171_C0822, R1 / ATPase
BMB171_C0823, R2 / Similar to BceSIV R1/R2
GCWGC or GCSGC
No companion M / Bth171ORF4698P, R3 / GCWGC or GCSGC
M.Bth171ORF2330P / YGGCCR (C5M) / No companion R
B. thuringiensis serovar konkukian 97-27 / BT9727_0840P, M1
BT9727_0841P, M2 / CCGG (C5M)
CCGG (C5M) / BT9727_0842 / CCGG (HpaII isoschizomer)
B. thuringiensis serovar konkukian 97-27 plasmid pBT9727 / M.BthKORF57P
(frame shift in TRD, motifs ix and x) / GGCC (C5M) / No companion R
B. thuringiensis serovar chinensis CT-43 / M.BthCT43ORF1818P / CCCGGG (N4C methylase)
(M.Cfr9I isoschizomer) / No companion R
B. thuringiensis serovar finitimus YBT-020 / No Type II R-M system
B. thuringiensis serovar finitimus YBT-020 plasmid pBMB28 / M.Bth20ORF29066P
(frame shift in TRD, motifs ix and x) / GGCC
Possible multi-specificity C5M / No companion R
a. Go to REBASE/Genomes with listing of predicted R and M genes in Bacillus strains. Click “closest neighbors” to find the sequence similarity to all known R and M genes.