Table S1 Number of Segregating AFLP Fragments by Primer Combination for Each of Both Crosses

Table S1 Number of segregating AFLP fragments by primer combination for each of both crosses (F1-2856 and F1-2872) for the species Picea glauca.

“-”, not tested.

Table S2 DNA amplification conditions from 35 SSR primer pairs previously developed from various Picea species.

a PCR programs as follows: (1) 4 min at 95°C for initial denaturation, 37 cycles of 45 s

at 94°C, 45 s at annealing temperature and 45 s at 72°C, followed by 10 min at 72°C;

(2) 5 min at 95°C, then 12 cycles of 45 s at 94°C, 45 s at 58°C (temperature decreasing by 0.5°C per cycle until 52°C) and 45 s at 72°C, followed by a second round of 27 cycles of 30 s at 94°C, 30 s at 52°C and 45 s at 72°C, followed by 10 min at 72°C; (3) 5 min at 94°C, then 33 cycles of 30 s at 94°C, 30 s at 60°C and 30 s at 72°C, followed by 5 min at 72°C; (4) 3 min at 94°C, then 2 cycles of 30 s at 94°C, 30 s at 60°C and 30 s at 72°C, followed by 25 cycles of 15 s at 94°C, 15 s at 54°C and 15 s at 72°C, followed by 3 min at 72°C.

b 1= Pfeifferet al.(1997); 2= Scottiet al.(2000); 3= Hodgettset al. (2001); 4= Rajoraet al.(2001); 5= Scottiet al.(2002a); 6= Scottiet al. (2002b); 7= Besnardet al.(2003).

Table S3 Number of markers genotyped for each of two crosses in Picea glauca.

a % markers with distorted segregation, significant at P ≤ 0.01 / number of tests (Bonferroni correction).

bIncluding 15 dominant SSR markers.

cIncluding 14 dominant SSR markers.

d Including 1 dominant ESTP marker.

Table S4 Primer sequences of Picea glauca used to amplify orthologous markers mapped in both Pseudotsuga menziesii and Pinus taeda.

a DNA amplification was carried out by an initial denaturation of 4 min at 95 °C, then 40 cycles of 30 s at 95 °C, 30 s at 60 °C and 1 min at 72 °C, followed by 10 min at 72 °C.

b Including intronic regions.

c Not taking into account the primer pair length.