Table S1: Morphological Characteristics of Yeast Species Isolated from Nectar of Hibiscus

Table S1: Morphological characteristics of yeast species isolated from nectar of Hibiscus rosa-sinensis flowers

No. / Yeast species / Colony color / Shape of cell / Pseudomycelium / Septate hyphae / Ballistoconidia / Symmetric conidia / Ascospores
Round,oval,conical or reniform / Ascospores cap,hat-,Saturm or walnut shaped
1 / Candida lusitaniae / wh.cr / gl-ov / - / - / - / - / + / -
2 / Candida parapsilosis / wh.cr / ov / + / - / - / - / - / -
3 / Debaromyces hansenii / wh.cr / r-ov / - / - / - / - / + / -
4 / Candida lambica / wh.cr / sph.ov / - / - / - / - / - / +
5 / Candida dubliensis / wh.cr / wr. / - / + / - / + / - / -
6 / Candida rugosa / wh.cr / + / - / - / - / - / -
7 / Candida magnoliae / wh.cr / gl-ov / - / - / - / - / - / -
8 / Candida guillermondii / wh.cr / ov.el / + / - / - / - / - / +
9 / Candida famata / wh.cr / sph.ov / - / - / - / - / - / -
10 / Candida tropicalis / wh.cr / ov / v / v / - / - / - / -
11 / Candida pseudotropicalis / Wh.cr-pi / ov / + / - / - / - / + / -
12 / Candida curvatus / br.yl / ov / + / - / - / - / - / -
13 / Sporobolomyces roseus / cr.pi-rd / sph-ov / - / +/- / + / - / - / -
14 / Pichia jadinii / wh.cr / sph-ov / - / - / - / - / - / +
15 / Hansenula beijerinckii / wh.cr / ov / + / - / - / - / - / +
16 / Hansenula polymorpha / tan.wh / sph-el / - / - / - / - / - / +
17 / Saccharomyces cerevisae / wh.cr / sph.ov / +/- / - / - / - / + / -
18 / Saccharomyces kluyveri / wh.cr / sph.ov / + / - / - / - / + / -

Colony color: wh: white; cr: cream; pi: pink; rd: red; yl: yellow;tan: tannish; Shape of cell: sph: spherical; ov: oval; r: round; fl: globose; wr: wrinkled; el:elongated.

hysiological /biochemical tests / Yeast species
1 / 2 / 3 / 4 / 5 / 6 / 7 / 8 / 9 / 10 / 11 / 12 / 13 / 14 / 15 / 16 / 17 / 18
Candida lusitaniae / Candida parapsilosis / Debaromyces hansenii / Candida lambica / Candida dubliensis / Candida rugosa / Candida magnoliae / Candida guillermondii / Candida famata / Candida tropicalis / Candida pseudotropicalis / Candida curvata / Sporobolomyces roseus / Pichia jadinii / Hansenula beijerinckii / Hansenula polymorpha / Saccharomyces cerevisae / Saccharomyces kluyveri
Fermentation
D-glucose fermentation / + / + / V
(+,-) / + / + / - / + / V
(+,D) / V
(+,-) / + / + / - / - / + / + / + / + / +
D-galactose fermentation / V
(+,-) / V
(-, D) / V
(-,D) / - / V
(+,-) / - / - / V
(-,D) / V
(-,D) / + / V
(+,D) / - / - / - / - / - / V
(+,-) / +
Maltose fermentation / V
(-,D) / V
(-, D) / V
(+,-) / - / + / - / - / - / V
(+,-) / + / - / - / - / - / - / V
(-,D) / V
(+,-) / V
(-,D)
Me α-D-glucoside fermentation / V
(-,D) / V
(-, D) / - / - / V
(-,D) / - / - / V
(+,-) / - / V
(-,D) / - / - / - / - / - / - / V
(+,-) / V
(+,-)
Sucrose fermentation / V
(-,D) / V
(-, D) / V
(+,-) / - / V
(-,D) / - / V
(+,D) / V
(+,D) / V
(+,-) / V
(+,-) / + / - / - / + / + / V
(-,D) / V
(+,-) / +
α - α-Trehalose fermentation / V
(+,D) / V
(-, D) / V
(-,D) / - / V
(+,-) / - / - / V
(-,D) / V
(-,D) / V
(+,D) / - / - / - / - / - / V
(+,-) / V
(+,-) / -
Melbiose fermentation / - / - / V
(-,D) / - / - / - / - / V
(-,D) / V
(-,D) / - / - / - / - / - / - / - / V
(+,-) / +
Lactose fermentation / - / - / - / - / - / - / - / - / - / - / V
(+,-) / - / - / - / - / - / - / -
Physiological /biochemical tests / Yeast species
1 / 2 / 3 / 4 / 5 / 6 / 7 / 8 / 9 / 10 / 11 / 12 / 13 / 14 / 15 / 16 / 17 / 18
Candida lusitaniae / Candida parapsilosis / Debaromyces hansenii / Candida lambica / Candida dubliensis / Candida rugosa / Candida magnoliae / Candida guillermondii / Candida famata / Candida tropicalis / Candida pseudotropicalis / Candida curvata / Sporobolomyces roseus / Pichia jadinii / Hansenula beijerinckii / Hansenula polymorpha / Saccharomyces cerevisae / Saccharomyces kluyveri
Cellobiose fermentation / V
(+,-) / - / V
(-,D) / - / - / - / - / V
(-,D) / V
(-,D) / - / - / - / - / - / D / V
(-,D) / - / -
Melezitose fermentation / V
(+,-) / V
(-, D) / V
(-,D) / - / - / - / - / - / V
(-,D) / V
(-,D) / - / - / - / - / - / - / V
(+,-) / -
Raffinose fermentation / - / - / V
(+,-) / - / - / - / - / V
(+,D) / V
(+,-) / - / V
(+,D) / - / - / V
(+,-) / + / - / V
(+,-) / +
Inulin fermentation / - / - / - / - / - / - / - / V
(-,D) / - / - / V
(+,-) / - / - / V
(-,D) / V
(+,-) / - / - / -
Starch fermentation / - / - / - / - / V
(-,D) / - / - / - / - / V
(+,-) / - / - / - / - / - / - / V
(+,-) / -
Assimilation of carbon compounds
D-glucose growth / + / + / + / + / + / + / + / + / + / + / + / + / + / N / + / + / + / +
D-galactose growth / V
(+,-) / + / + / - / + / V / V
(+,D) / + / + / + / + / + / V
(+,-) / - / - / V
(-,D) / V
(+,-) / +
L-sorbose growth / + / V
(+,D) / V
(+,D) / - / V
(+,-) / V / V
(+,D) / V
(+,D) / V / V
(+,-) / - / V
(-,D) / V
(+,-) / - / - / V
(-,D) / - / V
(+,-)
Physiological /biochemical tests / Yeast species
1 / 2 / 3 / 4 / 5 / 6 / 7 / 8 / 9 / 10 / 11 / 12 / 13 / 14 / 15 / 16 / 17 / 18
Candida lusitaniae / Candida parapsilosis / Debaromyces hansenii / Candida lambica / Candida dubliensis / Candida rugosa / Candida magnoliae / Candida guillermondii / Candida famata / Candida tropicalis / Candida pseudotropicalis / Candida curvata / Sporobolomyces roseus / Pichia jadinii / Hansenula beijerinckii / Hansenula polymorpha / Saccharomyces cerevisae / Saccharomyces kluyveri
D-glucosamine growth / V
(+,-) / V
(+, -) / V
(+,-) / V / V
(+,-) / - / - / + / V / V
(+,-) / - / V
(-,D) / - / - / - / - / - / -
D-ribose growth / V
(+,D) / V
(+, -) / V
(+,-) / - / V
(-,D) / - / V
(+,-) / V
(+,-) / V / V
(-,D) / V
(-,D) / + / V
(-,D) / - / - / + / - / V
D-xylose growth / + / + / + / + / + / + / V
(-,D) / + / + / + / V
(+,D) / + / V
(+,-) / + / + / V
(+,D) / - / V
(-,D)
L-arabinose growth / - / + / V
(+,-) / - / V
(+,-) / - / - / + / V
(+,-) / - / V
(+,-) / V
(-,D) / - / - / - / V
(-,D) / - / -
D-arabinose growth / V
(+,-) / - / V
(+,-) / - / V
(+,-) / - / - / + / V
(+,-) / - / - / V
(+,D) / V
(-,D) / - / - / V
(+,-) / - / -
L-rhamnose growth / + / - / V
(+,-) / - / - / - / - / V
(-,D) / V
(+,-) / - / - / V
(+,-) / - / - / - / V
(+,-) / - / -
Sucrose growth / + / + / + / - / V
(+,-) / - / V
(+,-) / + / + / V
(+,-) / + / + / + / + / + / + / V
(+,-) / +
Physiological /biochemical tests / Yeast species
1 / 2 / 3 / 4 / 5 / 6 / 7 / 8 / 9 / 10 / 11 / 12 / 13 / 14 / 15 / 16 / 17 / 18
Candida lusitaniae / Candida parapsilosis / Debaromyces hansenii / Candida lambica / Candida dubliensis / Candida rugosa / Candida magnoliae / Candida guillermondii / Candida famata / Candida tropicalis / Candida pseudotropicalis / Candida curvata / Sporobolomyces roseus / Pichia jadinii / Hansenula beijerinckii / Hansenula polymorpha / Saccharomyces cerevisae / Saccharomyces kluyveri
Maltose growth / + / + / + / - / + / - / - / + / + / + / - / V
(+,D) / + / + / + / + / V
(+,-) / +
α - α-Trehalose growth / + / + / + / - / V
(+,D) / - / V
(-,D) / + / + / + / - / D / V
(+,-) / + / + / + / V
(+,-) / V
(+,D)
Me α-D-glucoside growth / V
(+,D) / + / + / - / V
(+,-) / - / - / V
(+,D) / + / V / - / V
(+,-) / V
(+,-) / V
(+,D) / + / V
(+,-) / V
(+,-) / +
Cellobiose growth / V
(+,D) / - / V
(+,-) / - / - / - / V
(+,-) / + / V
(+,-) / V
(+,D) / V
(+,-) / + / V
(+,D) / + / + / V
(+,-) / - / V
(-,D)
Salicin growth / + / - / V
(+,-) / - / - / - / V
(+,-) / + / V
(+,-) / V
(+,-) / V
(+,D) / + / V
(+,D) / + / + / V
(+,-) / - / V
(-,D)
Arbutin growth / + / - / V
(+,-) / - / - / - / V
(+,-) / + / V
(+,-) / V
(+,D) / V
(+,-) / + / V
(+,D) / + / + / V
(+,-) / - / V
(-,D)
Melibiose growth / - / - / V
(+,-) / - / - / - / - / V
(+,-) / V
(+,-) / - / - / - / - / - / - / - / V
(+,-) / +
Lactose growth / - / - / V
(+,-) / - / - / - / - / - / V
(+,-) / - / V
(+,-) / + / - / - / - / - / - / -
Physiological /biochemical tests / Yeast species
1 / 2 / 3 / 4 / 5 / 6 / 7 / 8 / 9 / 10 / 11 / 12 / 13 / 14 / 15 / 16 / 17 / 18
Candida lusitaniae / Candida parapsilosis / Debaromyces hansenii / Candida lambica / Candida dubliensis / Candida rugosa / Candida magnoliae / Candida guillermondii / Candida famata / Candida tropicalis / Candida pseudotropicalis / Candida curvata / Sporobolomyces roseus / Pichia jadinii / Hansenula beijerinckii / Hansenula polymorpha / Saccharomyces cerevisae / Saccharomyces kluyveri
Raffinose growth / - / - / + / - / - / - / V
(+,-) / + / + / - / + / + / + / + / + / - / V
(+,-) / V
(+,D)
Melezitose growth / + / + / + / - / V
(+,-) / - / - / + / + / V
(+,-) / - / V
(+,-) / + / + / + / V
(+,-) / V
(+,-) / -
Inulin growth / - / - / V
(+,-) / - / - / - / - / + / V
(+,-) / - / V
(+,D) / - / - / V
(-,D) / V
(+,D) / - / - / -
Starch growth / - / - / V
(+,-) / - / + / - / - / - / V
(+,-) / + / - / V
(-,D) / V
(+,D) / - / - / - / V
(+,-) / -
Glycerol growth / + / + / + / + / V
(+,-) / + / + / V
(+,D) / + / V
(+,-) / V
(+,D) / + / V
(+,-) / + / + / + / V
(+,-) / V
(-,D)
Erythritol growth / - / - / V
(+,-) / - / - / - / - / - / V
(+,-) / - / - / V
(+,D) / - / - / - / + / - / -
Ribitol growth / + / V
(+,D) / + / - / V
(+,-) / V
(-,D) / V
(-,D) / + / + / V
(+,D) / V
(+,-) / V
(+,D) / V
(+,-) / - / - / + / - / -
Xylitol growth / + / V
(+,D) / + / - / + / V
(-,D) / V
(+,-) / + / + / V
(+,D) / V
(+,-) / V
(+,D) / V
(+,-) / V
(-,D) / + / + / - / V
(+,-)
Physiological /biochemical tests / Yeast species
1 / 2 / 3 / 4 / 5 / 6 / 7 / 8 / 9 / 10 / 11 / 12 / 13 / 14 / 15 / 16 / 17 / 18
Candida lusitaniae / Candida parapsilosis / Debaromyces hansenii / Candida lambica / Candida dubliensis / Candida rugosa / Candida magnoliae / Candida guillermondii / Candida famata / Candida tropicalis / Candida pseudotropicalis / Candida curvata / Sporobolomyces roseus / Pichia jadinii / Hansenula beijerinckii / Hansenula polymorpha / Saccharomyces cerevisae / Saccharomyces kluyveri-
L-arabinitol growth / - / V
(-,D) / V
(+,-) / - / - / - / - / + / V
(+,-) / - / - / V
(-,D) / - / - / - / + / - / -
D-glucitol growth / + / + / + / - / V
(+,D) / V
(+,D) / + / V
(+,D) / + / + / V
(+,D) / + / V
(+,-) / V
(+,-) / V
(+,D) / + / V
(-,D) / V
(+,D)
D-mannitol growth / + / + / + / - / + / V
(+,D) / + / V
(+,D) / + / + / V
(+,D) / V
(+,D) / V
(+,D) / V
(+,D) / V
(+,D) / + / V
(-,D) / V
(+,-)
Galactitol growth / - / - / V
(+,-) / - / - / - / - / V
(+,-) / V
(+,-) / - / - / D / - / - / - / V
(+,-) / - / -
Myo-Inositol growth / - / - / - / - / - / - / - / - / - / - / - / V
(+,-) / - / - / - / - / - / -
D-Glucono-1,5,-lactone growth / V
(+,D) / + / V
(+,-) / V / V
(+,-) / V
(+,-) / + / V
(+,-) / V
(+,-) / + / V
(-,D) / V
(+,-) / V
(+,-) / + / + / V
(+,-) / V
(-,D) / V
(-,D)
2-keto-D-gluconate growth / + / + / + / - / + / - / V
(+,D) / + / + / + / - / + / - / V
(-,D) / - / - / - / V
(-,D)
Physiological /biochemical tests / Yeast species
1 / 2 / 3 / 4 / 5 / 6 / 7 / 8 / 9 / 10 / 11 / 12 / 13 / 14 / 15 / 16 / 17 / 18
Candida lusitaniae / Candida parapsilosis / Debaromyces hansenii / Candida lambica / Candida dubliensis / Candida rugosa / Candida magnoliae / Candida guillermondii / Candida famata / Candida tropicalis / Candida pseudotropicalis / Candida curvata / Sporobolomyces roseus / Pichia jadinii / Hansenula beijerinckii / Hansenula polymorpha / Saccharomyces cerevisae / Saccharomyces kluyveri-
D-gluconate growth / V
(+,-) / V
(+,D) / V
(+,-) / - / V
(-,D) / V
(+,-) / + / V
(-,D) / V
(+,-) / V
(+,-) / - / + / V
(+,-) / V
(+,D) / + / - / - / V
(+,-)
D-glucuronate growth / - / - / V
(+,-) / - / - / - / - / - / V
(+,-) / - / - / + / - / - / - / - / - / -
D-galacturonate growth / - / - / - / - / - / - / - / - / - / - / - / - / - / - / N / V
(-,D) / - / -
DL-Lactate growth / V
(+,-) / - / V
(+,-) / + / + / V / - / - / V
(+,-) / V
(+,-) / + / V
(+,D) / V
(+,-) / + / + / - / V
(+,-) / V
(+,-)
Succinate growth / + / + / + / + / + / V
(+,-) / V
(+,D) / + / + / + / + / + / + / + / + / - / V
(-,D) / V
(+,D)
Citrate growth / V
(+,-) / + / + / + / + / V
(+,-) / V
(+,D) / + / + / V
(+,D) / V
(+,-) / + / V
(-,D) / + / - / V
(-,D) / - / -
Methanol growth / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / V
(+,-) / - / -
Ethanol growth / + / + / + / + / + / + / V
(+,-) / + / + / + / + / V
(+,D) / V
(+,-) / + / + / V
(+,-) / V
(+,-) / +
Physiological /biochemical tests / Yeast species
1 / 2 / 3 / 4 / 5 / 6 / 7 / 8 / 9 / 10 / 11 / 12 / 13 / 14 / 15 / 16 / 17 / 18
Candida lusitaniae / Candida parapsilosis / Debaromyces hansenii / Candida lambica / Candida dubliensis / Candida rugosa / Candida magnoliae / Candida guillermondii / Candida famata / Candida tropicalis / Candida pseudotropicalis / Candida curvata / Sporobolomyces roseus / Pichia jadinii / Hansenula beijerinckii / Hansenula polymorpha / Saccharomyces cerevisae / Saccharomyces kluyveri-
Propane 1,2 diol growth / V
(+,D) / V
(-,D) / - / - / V
(+,-) / V
(+,D) / V
(+,-) / V
(-,D) / - / V
(-,D) / - / V
(+,-) / V
(-,D) / + / N / + / - / -
Butane 2,3 diol growth / - / - / - / - / V
(+,-) / + / V
(+,-) / - / - / - / + / + / - / + / N / + / - / +
Assimilation of nitrogen compounds
Nitrate growth / - / - / - / - / - / - / + / - / - / - / - / - / V
(+,-) / + / + / + / - / -
Nitrite growth / - / - / V
(+,-) / - / - / - / + / V
(+,-) / V
(+,-) / - / - / V
(+,-) / V
(+,-) / + / + / + / - / -
Ethylamine growth / + / + / + / + / + / + / + / + / + / + / + / + / V
(+,-) / + / + / + / - / +
L-lysine growth / V
(+,-) / + / + / + / + / + / + / + / + / + / + / + / V
(+,-) / + / + / + / - / +
Cadaverine growth / + / + / + / + / + / + / + / + / + / + / + / + / V
(+,-) / + / + / + / - / +
Creatine growth / - / - / V
(+,-) / - / - / - / - / - / V
(+,-) / - / - / - / - / - / - / - / - / -
Physiological /biochemical tests / Yeast species
1 / 2 / 3 / 4 / 5 / 6 / 7 / 8 / 9 / 10 / 11 / 12 / 13 / 14 / 15 / 16 / 17 / 18
Candida lusitaniae / Candida parapsilosis / Debaromyces hansenii / Candida lambica / Candida dubliensis / Candida rugosa / Candida magnoliae / Candida guillermondii / Candida famata / Candida tropicalis / Candida pseudotropicalis / Candida curvata / Sporobolomyces roseus / Pichia jadinii / Hansenula beijerinckii / Hansenula polymorpha / Saccharomyces cerevisae / Saccharomyces kluyveri
Creatinine growth / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / -
Glucosamine growth / - / - / - / + / - / - / - / V
(+,-) / - / - / - / - / - / - / - / - / - / -
Imidazole growth / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / -
Growth without vitamins
Growth w/o vitamins / - / - / V
(+,-) / - / V
(-,D) / - / - / V
(-,D) / V
(+,-) / V
(+,-) / - / - / V
(+,-) / + / + / - / V
(+,-) / V
(+,D)
Growth w/o myo-Inositol / + / + / + / + / + / + / + / + / + / + / + / + / + / + / + / + / V
(+,-) / V
(+,D)
Growth w/o pantothenate / + / + / + / + / + / + / + / + / + / + / V
(+,-) / + / + / + / + / + / V
(+,-) / +
Growth w/o biotin / - / - / V (+,-) / + / V
(-,D) / V
(+,-) / V
(+,-) / V
(-,D) / V
(+,-) / V
(+,-) / V
(-,D) / V
(+,-) / + / + / + / - / V
(+,-) / V
(+,D)
Growth w/o thiamin / - / + / + / - / + / - / - / + / + / + / + / + / V
(+,-) / + / + / + / V
(+,-) / +
Physiological /biochemical tests / Yeast species
1 / 2 / 3 / 4 / 5 / 6 / 7 / 8 / 9 / 10 / 11 / 12 / 13 / 14 / 15 / 16 / 17 / 18
Candida lusitaniae / Candida parapsilosis / Debaromyces hansenii / Candida lambica / Candida dubliensis / Candida rugosa / Candida magnoliae / Candida guillermondii / Candida famata / Candida tropicalis / Candida pseudotropicalis / Candida curvata / Sporobolomyces roseus / Pichia jadinii / Hansenula beijerinckii / Hansenula polymorpha / Saccharomyces cerevisae / Saccharomyces kluyveri-
Growth w/o biotin & thiamin / - / - / V
(+,-) / - / V
(-,D) / - / - / V
(-,D) / V
(+,-) / V
(+,-) / V
(+,-) / - / V
(+,-) / + / + / - / V
(+,-) / V
(+,D)
Growth w/o pyridoxine / - / + / + / + / + / + / + / + / + / + / + / + / + / + / + / + / V
(+,-) / +
Growth w/o niacin / + / + / + / + / + / + / + / + / + / + / - / + / + / + / + / + / + / +
Growth w/o PABA / + / + / + / + / + / + / + / + / + / + / + / + / + / + / + / + / V
(+,-) / +
Growth at different temperatures
growth at 25°C / + / + / + / + / + / + / + / + / + / + / + / + / + / + / + / + / + / +
growth at 30°C / + / + / + / + / + / + / + / + / + / + / + / + / V
(+,-) / + / + / + / + / +
Physiological /biochemical tests / Yeast species
1 / 2 / 3 / 4 / 5 / 6 / 7 / 8 / 9 / 10 / 11 / 12 / 13 / 14 / 15 / 16 / 17 / 18
Candida lusitaniae / Candida parapsilosis / Debaromyces hansenii / Candida lambica / Candida dubliensis / Candida rugosa / Candida magnoliae / Candida guillermondii / Candida famata / Candida tropicalis / Candida pseudotropicalis / Candida curvata / Sporobolomyces roseus / Pichia jadinii / Hansenula beijerinckii / Hansenula polymorpha / Saccharomyces cerevisae / Saccharomyces kluyveri-
growth at 35°C / + / + / V
(+,-) / + / + / + / + / + / V
(+,-) / + / + / V
(+,-) / - / + / V / + / V
(+,-) / +
growth at 37°C / + / + / V
(+,-) / + / + / + / + / + / V
(+,-) / + / + / - / - / + / - / + / V
(+,-) / +
growth at 40°C / V
(+,W) / V
(+,-) / - / - / + / V
(+,-) / - / V
(+,-) / - / + / + / - / - / V
(+,-) / - / + / V
(+,-) / -
Additional tests
0.01% Cyclohex. Growth / - / V
(+,-) / V
(+,-) / - / + / - / V
(-,D) / + / V
(+,-) / + / + / V
(+,-) / V
(-,D) / - / - / + / - / -
0.1% Cyclohex.growth / - / - / V
(+,-) / - / + / - / - / + / V
(+,-) / + / + / V
(-,D) / - / - / - / + / - / -
1.0% acetic acid growth / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / -
50% D-glucose growth / V
(+,D) / + / + / V / V
(+,-) / D / + / V
(+,D) / + / V
(+,D) / V
(-,D) / - / - / V
(+,-) / - / V
(+,D) / V
(+,-) / V
(+,-)
Physiological /biochemical tests / Yeast species
1 / 2 / 3 / 4 / 5 / 6 / 7 / 8 / 9 / 10 / 11 / 12 / 13 / 14 / 15 / 16 / 17 / 18
Candida lusitaniae / Candida parapsilosis / Debaromyces hansenii / Candida lambica / Candida dubliensis / Candida rugosa / Candida magnoliae / Candida guillermondii / Candida famata / Candida tropicalis / Candida pseudotropicalis / Candida curvata / Sporobolomyces roseus / Pichia jadinii / Hansenula beijerinckii / Hansenula polymorpha / Saccharomyces cerevisae / Saccharomyces kluyveri-
60% D-glucose growth / - / - / V
(+,-) / - / - / - / + / - / V
(+,-) / - / - / - / - / - / - / - / - / -
Starch formation / - / - / - / - / - / - / - / - / - / - / - / V
(-,W) / - / - / - / - / - / -
Acetic acid production / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / - / -
Urea hydrolysis / - / - / - / - / - / - / - / - / - / - / - / + / + / - / - / - / - / -
Diazonium Blue B reaction / - / - / - / - / - / - / - / - / - / - / - / + / + / - / - / - / - / -

+ Positive; - Negative; V Variation; D Delayed growth after seven days; WWeak utilization

* The results presented here are the average values of the experiment performed therefore in certain biochemical tests variation was observed by certain yeasts species.

Table S3: Prevalence of yeasts in the nectary glands of flowers (ten samples examined from Hibiscus rosa sinensis)

No. / Yeast species / Positive samples
Number (n)
1 / Candida lusitaniae / 7.0
2 / Candida parapsilosis / 7.0
3 / Debaromyces hansenii / 5.0
4 / Candida lambica / 4.0
5 / Candida dubliensis / 4.0
6 / Candida rugosa / 5.0
7 / Candida magnoliae / 6.0
8 / Candida guillermondii / 7.0
9 / Candida famata / 7.0
10 / Candida tropicalis / 6.0
11 / Candida pseudotropicalis / 6.0
12 / Candida curvata / 9.0
13 / Sporobolomyces roseus / 8.0
14 / Pichia jadinii / 6.0
15 / Hansensula beijerinckii / 7.0
16 / Hansensula polymorpha / 8.0
17 / Saccharomyces cerevisae / 6.0
18 / Saccharomyces kluyveri / 6.0

Table S4: The colony characteristics of Candida sp. on Sabouraud medium and on BCG CHROM Candida agar base

No. / Yeast species / Colony characteristics
Sabouraud Dextrose agar base / BCG CHROM agar base
1 / Candida lusitaniae / White to cream, glistening, and smooth. Colonies are fringed with pseudohyphae. / Pink, mucoid
2 / Candida parapsilosis / Colonies are white, creamy, shiny, and smooth / White, sometimes mucoid
3 / Debaromyces hansenii / Yellow,dull,smooth / Bluish purple
4 / Candida lambica / White to cream in color, smooth / Pink colored colonies
5 / Candida dubliensis / White, smooth and glistening / Dark green colored colonies
6 / Candida rugosa / White to cream in color, wrinkled / Light blue-green, flat, rough
7 / Candida magnoliae / White to cream in color / ----
8 / Candida guillermondii / Colonies are flat, moist, smooth, and creamish yellow in color / Pink-lavender, mucoid
9 / Candida famata / Grayish white to yellowish dull wrinkled / White pink
10 / Candida tropicalis / Cream colored with slightly mycelial border / Steel blue purple
11 / Candida pseudotropicalis / Cream colored / Steel blue purple
12 / Candida curvata / Brown to yellow in color / ---

Table S5: Physiological characterization of yeast species as identified through KB006 Hi CandidaTM Identification Kit

Tests / Yeasts isolates
1 / 2 / 3 / 4 / 5 / 6 / 7 / 8 / 9 / 10 / 11 / 12
Urease / - / - / - / - / - / - / - / - / - / - / - / +
Melibiose / - / - / V
(+,-) / - / - / - / - / V
(+,-) / V
(+,-) / - / - / -
Lactose / - / - / V
(+,-) / - / - / - / - / - / V
(+,-) / - / V
(+,-) / +
Maltose / + / + / + / - / + / - / - / + / + / + / - / +
Sucrose / + / + / + / - / V
(+,-) / - / V
(+,-) / + / + / V
(+,-) / + / +
Galactose / + / + / + / - / + / - / + / + / + / + / + / +
Cellobiose / + / - / V
(+,-) / - / - / - / V
(+,-) / + / V
(+,-) / + / V
(+,-) / +
Inositol / - / - / - / - / - / - / - / - / - / - / - / +
Xylose / + / + / + / + / + / + / -,D / + / + / + / + / +
Dulcitol / + / + / + / - / + / + / + / + / + / + / + / +/-
Raffinose / - / - / + / - / - / - / V
(+,-) / + / + / - / + / +
Trehalose / + / + / + / - / + / - / V
(-,D) / + / + / + / - / D

1. Candida lusitaniae; 2. Candida parapsilosis; 3. Debaromyces hansenii; 4. Candida lambica; 5. Candida dubliensis;6. C.rugosa; 7. Candida magnoliae; 8. Candida guillermondii 9. Candida famata ; 10. Candida tropicalis; 11. Candida pseudotropicalis; 12. Candida curvata

Note: Based on the % strains showing reactions following symbols have been assigned from laboratory result and standard references.

+ = Positive reaction (more than 90%) ; - = Negative reaction; +/- = variable; n= not determined; D = Delayed growth after seven days.