Clade / ITS2 Type / Genbank number / Pseudonyms / Locations detected in
this study / Previously reported in
O. annularis / References
B / B1 / AF333511 / Symbiodinium pulchrorum;
S. bermudense / Bahamas; Barbados; Belize; Cayman Islands; Columbia; Cuba; Curacao; Dominican Rep.; Honduras; Jamaica; Nicaragua; Tobago; US Virgin Islands; Venezuela / Exuma Islands & Florida Keys; Barbados; Bahamas; US Virgin Islands; Bermuda / LaJeunesse 2002; Savage et al. 2002; Thornhill et al. 2006; Correa et al. 2009; Thornhill et al. 2009; Finney et al. 2010
B10 / AF499787 / Cayman Islands; Cuba / Florida Keys / Thornhill et al. 2009
B17 / AY074987 / Belize / Panama / Savage et al. 2002
B1j / GU907637 / Barbados; Belize; Curacao; Venezuela / Eastern Caribbean / LaJeunesse et al. 2009; Finney et al. 2010;
C / C1 / AF333515 / Symbiodinium goreaui / Bahamas; Cuba; Honduras; Tobago / US Virgin Islands / Correa et al. 2009
C3 / AF499789 / Belize; Columbia; Dominica / US Virgin Islands; Exuma Islands; Florida Keys / LaJeunesse 2002; Thornhill et al. 2006; Warner et al. 2006; Correa et al. 2009; Thornhill et al. 2009
C7 / AF499797 / Bahamas; Barbados; Cayman Islands; Cuba; Curacao; Dominica; Dominican Rep.; Jamaica; Nicaragua; US Virgin Islands; Tobago; Venezuela / US Virgin Islands; Barbados / Warner et al. 2006; Correa et al. 2009; LaJeunesse et al. 2009; Finney et al. 2010
C12 / AF499801 / C7a / Bahamas; Barbados; Cuba; Curacao; Dominica; Dominican Rep.; Nicaragua; US Virgin Islands; Tobago; Venezuela / Eastern Caribbean; Exhuma Islands / Thornhill et al. 2006; LaJeunesse et al. 2009; Thornhill et al. 2009; Finney et al. 2010;
D / D1 / AF334660 / Symbiodinium glynni / n/a / US Virgin Islands; South Yucatan; Exuma Islands; Florida Keys / LaJeunesse et al. 2001; LaJeunesse 2002; Thornhill et al. 2006; Correa et al. 2009; LaJeunesse et al. 2009; Finney et al. 2010
D1-4 / AF499802 / Symbiodinium trenchii, D1b, D5, D1a / Bahamas; Belize; Cuba; Dominica; Honduras; US Virgin Islands / Exuma Islands; US Virgin Islands; South Yucatan; Florida Keys / LaJeunesse 2002; Thornhill et al. 2006; Correa et al. 2009; Finney et al. 2010; LaJeunesse et al. 2009

Table S1. Dominant Symbiodinium ITS2 types identified by DGGE analysis in this study, along with GenBank accession numbers and details on sampling locations from this and previous studies.

References

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Finney J, Pettay D, Sampayo E, Warner M, Oxenford H, LaJeunesse T (2010) The relative significance of host–habitat, depth, and geography on the ecology, endemism, and speciation of coral endosymbionts in the genus Symbiodinium. Microbial Ecology 60:250-263

LaJeunesse TC (2001) Investigating the biodiversity, ecology, and phylogeny of endosymbiotic dinoflagellates in the genus Symbiodinium using the ITS region: In search of a "species" level marker. Journal of Phycology 37:866-880

LaJeunesse TC (2002) Diversity and community structure of symbiotic dinoflagellates from Caribbean coral reefs. Marine Biology 141:387-400

LaJeunesse TC, Smith RT, Finney J, Oxenford H (2009) Outbreak and persistence of opportunistic symbiotic dinoflagellates during the 2005 Caribbean mass coral 'bleaching' event. Proceedings Biological sciences: The Royal Society 276:4139-4148

Savage AM, Trapido-Rosenthal H, Douglas AE (2002) On the functional significance of molecular variation in Symbiodinium, the symbiotic algae of Cnidaria: photosynthetic response to irradiance. Marine Ecology Progress Series 244:27–37

Thornhill DJ, LaJeunesse TC, Kemp DW, Fitt WK, Schmidt GW (2006) Multi-year, seasonal genotypic surveys of coral-algal symbioses reveal prevalent stability or post-bleaching reversion. Marine Biology 148:711-722

Thornhill DJ, Xiang Y, Fitt WK, Santos SR (2009) Reef endemism, host specificity and temporal stability in populations of symbiotic dinoflagellates from two ecologically dominant Caribbean corals. PLoS ONE 4:e6262

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