Table S1. Characteristics of field site samples sampled adjacent to wells.

Well / Location / Depth
(m) / % Sand / % Silt / % Clay / Class / Cation Exchange Capacity (cmol/kg)a / TNT (mg/kg) / ORPb (mV) / pHb / Fe2+ (g/kg) / Bioavailable Fe3+ c (g/kg)
A / Source zone / 5.79 / 80.9 / 8.0 / 11.1 / Loamy sand / -- / 3406. / -- / -- / 0.87 / 19.0+/-2.3
B / Outside source zone / 4.27 / 79.9 / 7.5 / 12.6 / Sandy loam / 14.8 / 20.7 / 398 / 4.4 / 0.06 / 8.9+/-4.5
C / Upgradient of source zone / 4.88 / 81.2 / 7.0 / 11.9 / Sandy loam / 16.4 / b.d.d / -- / -- / 1.82 / 13.2+/-12.9

a =EPA Method 8091, b= Field measurement, cBioavailable Ferric Iron Assay (New Horizons, Baltimore, MD). , d= below detection.

Table S2. Comparison of zero, first, and second order model parameters and fit.

Zero Order1 / First Order2 / Second Order3
Test condition / Replicate ID / k0
(µM/hr) / R2 / k1
(hr-1) / R2 / k2
(µM-1hr-1) / R2
Lactate / a / -1.310-1 / 0.94 / -4.310-2 / 0.83 / 3.110-2 / 0.53
b / -1.510-1 / 0.95 / -3.410-2 / 0.78 / 1.110-2 / 0.58
c / -1.510-1 / 0.95 / -4.810-2 / 0.73 / 3.310-2 / 0.45
d / -1.210-1 / 0.91 / -3.610-2 / 0.91 / 1.910-2 / 0.72
Ethanol / a / -9.410-2 / 0.98 / -2.210-2 / 0.90 / 7.010-3 / 0.68
b / -9.010-2 / 0.98 / -2.310-2 / 0.88 / 9.110-3 / 0.64
c / -8.510-2 / 0.98 / -1.910-2 / 0.94 / 5.310-3 / 0.80
d / -8.410-2 / 0.99 / -2.110-2 / 0.90 / 6.910-3 / 0.69
e / -8.210-2 / 0.98 / -1.410-2 / 0.89 / 3.110-3 / 0.73
PBOC / a / -5.610-2 / 0.99 / -1.310-2 / 0.93 / 3.910-3 / 0.72
b / -5.110-2 / 0.98 / -1.410-2 / 0.91 / 5.910-3 / 0.64
c / -5.410-2 / 0.95 / -1.310-2 / 0.94 / 4.610-3 / 0.65
d / -2.610-2 / 0.89 / -1.010-1 / 0.75 / 4.810-3 / 0.66
e / -4.810-2 / 0.98 / -1.110-2 / 0.92 / 3.710-3 / 0.76

1 k0 is the slope found plotting Concentration of TNT versus time (hrs), 2 k1 is found plotting ln(TNT concentration) versus time (hrs), 3 k2 is the slope found by plotting 1/(TNT concentration) versus time.

Table S3. Highest sequence similarities found for DGGE bands

Band / Phylum / Class / Highest 16S rRNA Sequence Similarity from BLAST (accession number) / % Match
a / Proteobacteria / γ -proteobacteria / Pseudomonas sp. (FM161516) / 94%
b / Proteobacteria / γ -proteobacteria / Uncultured soil bacterium clone GO0VNXF07H8RDO, partial sequence. (JF383105) / 89%
c / Firmicutes / Negativicutes / Uncultured Pelosinus sp. clone PAGW1_64 (GQ257723) / 89%
d / Proteobacteria / γ -proteobacteria / Uncultured Pseudomonas sp. clone LJH D, partial sequence (HM192823) / 93%
e / Firmicutes / Clostridia / Desulfitobacterium sp. CR1 (AB299028) / 95%
f / Firmicutes / Clostridia / Eubacterium saburreum clone ATC_H74_12 (GU413453) / 98%
g / Proteobacteria / γ -proteobacteria / UnculturedPseudomonas sp. clone adm 34, partial sequence (JF812373) / 96%
h / Proteobacteria / β-proteobacteria / Ralstonia sp. LIG5GB, partial sequence. (HM587786) / 99%
i / Proteobacteria / β -proteobacteria / Uncultured beta proteobacterium clone THz2 30, partial sequence. (HM854322) / 96%
j / Firmicutes / Clostridia / Eubacterium saburreum clone ATC_H74_12, partial sequence (GU413453) / 98%
k / Proteobacteria / β -proteobacteria / Cupriavidus basilensis isolate TFD8 (EF177353) / 97%
l / Proteobacteria / β proteobacteria / Methylobacterium sp. T34 partial, strain T34 (FR869835) / 95%
m / Proteobacteria / β -proteobacteria / Cupriavidus sp. DE7, partial sequence (JN226398) / 97%
n / Proteobacteria / β -proteobacteria / Uncultured Betaproteobacteria bacterium from clone (CU920167) / 97%