Comparative characterization, expression pattern and function analysis of the 12-oxo-phytodienoic acid reductase gene family in rice

Wenyan Li1, Feng Zhou2, Bing Liu1, Dongru Feng1, Yanming He1, Kangbiao Qi1,

Hongbin Wang1* and Jinfa Wang1

1, State Key Laboratory for Biocontrol and Key Laboratory of Gene Engineering of Ministry of Education, School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, P. R. China

2, College of Life Science, South China Agricultural University, 510642, Guangzhou, P. R. China

*Corresponding author

Hongbin Wang, Tel/Fax: +862084039179; E-mail:


Table S1 OsOPR family genes and their biochemical properties.

Subfamily / Locus Namea / Chromosome / Gene Name / FL_cDNAc / A.Ad / MWe / pIe
Ⅰ / Not assigned / 04 / OsOPR04-1
(OsOPR11) / AK102440 / 367 / 40642.88 / 6.26
Ⅱ / LOC_Os08g35740
(Os08g0459600) / 08 / OsOPR08-1
(OsOPR7b) / AK071203 / 394 / 43677.45 / 8.72
Ⅲ / LOC_Os06g11200
(Os06g0215500) / 06 / OsOPR06-1
(OsOPR6b) / AK108079 / 391 / 43513.56 / 5.34
LOC_Os06g11210
(Os06g0215600) / 06 / OsOPR06-2
(OsOPR5b) / AK061212, AK104039, AK104193, AK104256, EU146301 / 374 / 41684.87 / 5.25
LOC_Os06g11240
(Os06g0215900) / 06 / OsOPR06-3
(OsOPR4b) / AK105590, AK072596 / 380 / 42431.89 / 5.91
LOC_Os06g11260 (Os06g0216000) / 06 / OsOPR06-4
(OsOPR3b) / AK287507 / 382 / 42408.80 / 6.12
LOC_Os06g11280
(Os06g0216200) / 06 / OsOPR06-5
(OsOPR2b) / EST / 378 / 42068.40 / 6.11
LOC_Os06g11290
(Os06g0216300) / 06 / OsOPR06-6
(OsOPR1b) / AK103067, AB040743, AJ557138, AJ557139 / 380 / 42465.02 / 5.79
Ⅳ / LOC_Os01g27230
(Os01g0369900) / 01 / OsOPR01-1
(OsOPR10b) / AK100034 / 372 / 41077.35 / 5.89
LOC_Os01g27240
(Os01g0370000) / 01 / OsOPR01-2
(OsOPR9b) / AK121554 / 367 / 40748.76 / 5.58
Ⅴ / LOC_Os02g35310
(Os02g0559400) / 02 / OsOPR02-1
(OsOPR8b) / AK069898,
AK059887 / 365 / 41061.59 / 6.01
Not assigned / 04 / OsOPR04-2
(OsOPR12) / AK106409 / 390 / 41881.92 / 5.02
Not assigned / 04 / OsOPR04-3
(OsOPR13) / AK073836 / 376 / 41951.27 / 5.18

a, Locus of OPR genes were assigned in TIGR(RAP-DB); Three sequences located on the gap of chromosome 04 and not assigned in the databases (i.e. AK102440, AK106409, AK0676218) were also listed and named as OsOPR11, OsOPR12 and OsOPR13, respectively.

b, Gene numbering was based on the report by Tani et al.(2008).

c, FL_cDNA (full-length cDNA) clones used in this study were obtained from The National Institute of Agrobiological Sciences (NIAS) of Japan; Only LOC_Os06g11280(Os06g0216200) was reported with EST, but without full-length cDNA.

d, A.A, the number of amino acid;

e, MW and pI were computed by pI/Mw tool (http://www.expasy.ch/tools/pi_tool.html).

References

Tani T, Sobajima H, Okada K, Chujo T, Arimura S, Tsutsumi N, Nishimura M, Seto H, Nojiri H, Yamane H. 2008. Identification of the OsOPR7 gene encoding 12-oxophytodienoate reductase involved in the biosynthesis of jasmonic acid in rice. Planta 227, 517-526.